Objective:To investigate the value of electrical cardiometry(EC),a noninvasive hemodynamic testing technique,in the di-agnosis of heart failure(HF)in adults.Methods:A prospective study was conducted among the adult patients who were hospitalized in Department of Cardiology,The First Affiliated Hospital of Chongqing Medical University,from September 2022 to May 2024,and the patients who were diagnosed with HF were enrolled as HF group,while those who were excluded from the clinical diagnosis of HF were enrolled as control group.The two groups were compared in terms of general clinical data and related parameters measured by EC,in-cluding stroke volume variation(SVV),systolic time ratio(STR),pre-ejection period(PEP),index of contractility(ICON),ICON varia-tion(VIC),left ventricular ejection time(LVET),and left ventricular ejection fraction(LVEF).A multivariate logistic regression model established by the forward method was used to investigate the factors associated with HF.The receiver operating characteristic(ROC)curve was plotted for the hemodynamic parameters measured by EC in the diagnosis of HF,and the area under the ROC curve(AUC)was calculated.Results:A total of 239 patients were enrolled,with 126 in the HF group and 113 in the control group.Compared with the control group,the HF group had significantly higher SVV,STR,PEP,VIC,and age and significantly lower ICON,LVET,and LVEF(all P<0.001).The multivariate logistic regression model estab-lished by the forward method showed that STR(odds ratio[OR]=1.199,95%CI=1.110-1.294,P<0.001),VIC(OR=1.176,95%CI=1.090-1.269,P<0.001),and age(OR=1.068,95%CI=1.010-1.128,P<0.05)were positively correlated with HF,and ICON(OR=0.968,95%CI=0.941-0.996,P<0.05)and LVEF(OR=0.854,95%CI=0.798-0.913,P<0.001)were negatively correlated with HF.The ROC curve analysis showed that STR,VIC,and ICON had an AUC of 0.887(95%CI=0.848-0.927),0.891(95%CI=0.851-0.932),and 0.718(95%CI=0.654-0.782),respectively,in the diagnosis of HF,with an optimal cut-off value of 37.5%,19.5%,and 49.3,respec-tively,a sensitivity of 91.3%,73.8%,and 50.0%,respectively,and a specificity of 61.4%,93.8%,and 86.7%,respectively.Among these indicators,STR combined with VIC had an AUC of 0.940(95%CI=0.912-0.967)in the diagnosis of HF,with a sensitivity of 83.3%and a specificity of 91.2%.Conclusion:The EC method can effectively assess the cardiac functional status of adult patients,and STR combined with VIC has some clinical value for the diagnosis of heart failure.

Humanistic caring for patients in the operating room refers to providing the whole process of caring medical services for patients in the operating room. In order to standardize humanistic caring services for patients in the operating room of medical institutions, improve the comprehensive service level of the operating room, and enhance the surgical experience of patients, the Chinese Association for Life Care released the group standard " Humanistic Caring Management Standards for Patients in the Operating Room" in December 2023. This article interpreted the basic requirements for humanistic caring of patients in the operating room, the environment and facilities for humanistic caring, the procedures and measures for humanistic caring, and the quality management framework, aiming to assist administrators and clinical practitioners across various levels of medical institutions in accurately understanding and effectively implementing the standard, and to provide essential textual reference and practical guidance for promoting the application of the standard.

Astragali Radix (AR), a traditional Chinese medicine (TCM), has demonstrated therapeutic efficacy against various diseases, including cardiovascular conditions, over centuries of use. While doxorubicin serves as an effective chemotherapeutic agent against multiple cancers, its clinical application remains constrained by significant cardiotoxicity. Research has indicated that AR exhibits protective properties against doxorubicin-induced cardiomyopathy (DIC); however, the specific bioactive components and underlying mechanisms responsible for this therapeutic effect remain incompletely understood. This investigation seeks to identify the protective bioactive components in AR against DIC and elucidate their mechanisms of action. Through network medicine analysis, astragaloside IV (AsIV) and formononetin (FMT) were identified as potential cardioprotective agents from 129 AR components. In vitro experiments using H9c2 rat cardiomyocytes revealed that the AsIV-FMT combination (AFC) effectively reduced doxorubicin-induced cell death in a dose-dependent manner, with optimal efficacy at a 1∶2 ratio. In vivo, AFC enhanced survival rates and improved cardiac function in both acute and chronic DIC mouse models. Additionally, AFC demonstrated cardiac protection while maintaining doxorubicin's anti-cancer efficacy in a breast cancer mouse model. Lipidomic and metabolomics analyses revealed that AFC normalized doxorubicin-induced lipid profile alterations, particularly by reducing fatty acid accumulation. Gene knockdown studies and inhibitor experiments in H9c2 cells demonstrated that AsIV and FMT upregulated peroxisome proliferator activated receptor γ coactivator 1α (PGC-1α) and PPARα, respectively, two key proteins involved in fatty acid metabolism. This research establishes AFC as a promising therapeutic approach for DIC, highlighting the significance of multi-target therapies derived from natural herbals in contemporary medicine.
Animals ; Doxorubicin/adverse effects* ; Saponins/administration & dosage* ; Isoflavones/pharmacology* ; Rats ; Cardiomyopathies/prevention & control* ; Mice ; Fatty Acids/metabolism* ; Myocytes, Cardiac/metabolism* ; Triterpenes/administration & dosage* ; Male ; Drugs, Chinese Herbal/administration & dosage* ; Humans ; Cardiotonic Agents/administration & dosage* ; Mice, Inbred C57BL ; Cell Line ; Astragalus Plant/chemistry* ; Astragalus propinquus

Objective To investigate the epidemiological trends,temporal and spatial distribution characteristics of pulmonary tuberculosis in Lanping County.Methods Based on tuberculosis management data and basic information systems from the"China Disease Prevention and Control Information System,"pulmonary tuberculosis data from Lanping County for 2018-2023 were obtained.Descriptive epidemiology,concentration method,circular distribution method,and spatial autocorrelation analysis were used to conduct epidemiological and spatial analyses of the pulmonary tuberculosis data.Results A total of 2836 TB cases were reported in Lanping County from 2018 to 2023,with an average annual incidence rate of 233.26 per 100000,showing a declining trend.The male-to-female ratio was 1.95∶1,with the highest incidence among individuals aged 60 and above(932 cases,32.86%).Cases were predominantly among farmers(91.01%)and the Lisu ethnic group(52.68%).TB incidence showed weak seasonality with a bimodal distribution,with primary peak occurring from October to March and secondary peak from June to August.Tu'e Township(324.74 per 100,000),Shideng Township(307.42 per 100000),and Jinding Town(260.98 per 100,000)had the highest incidence rates,accounting for 1,284 cases or 45.28%of the county's total cases.In 2020,the incidence of pulmonary tuberculosis in Lanping County showed a spatial clustering distribution(global Morans's I value<0,P value<0.05),with Shideng Township consistently showing high-low aggregation characteristics.Conclusion Between 2018-2023,while the tuberculosis incidence rate in Lanping County has declined,it still falls short of Yunnan Province's tuberculosis prevention and control targets,and the prevention and control work continues to face significant challenges.Strengthening screening of high-risk populations and providing medical support to remote areas will be key measures for future prevention and treatment.

Objective: To investigate the value of metagene next⁃generation sequencing ( mNGS) in the detection of pathogens in patients with pulmonary infection. Methods: A retrospective analysis was performed on clinical data from 434 patients with pulmonary infections admitted over the past four years. Based on the presence of underlying comorbidities , patients were divided into underlying disease group (n = 262) and non⁃underlying disease group (n = 172) . Pathogen detection was conducted using both mNGS and conventional tests. Clinical and laboratory parameters , radiographic findings , and pathogen detection results were systematically analyzed. The diagnostic performance of the two methods in identifying causative pathogens of pulmonary infections was compared. Results: The positive rate of mNGS in 434 patients was higher than that of conventional tests , and the difference was statisti⁃cally significant (P < 0. 05) . The efficacy of mNGS in detecting bacteria and viruses was significantly higher than that of conventional tests , and the difference was statistically significant (P < 0. 05) . Although the fungal detection rate of mNGS was higher than that of conventional tests , the difference was not statistically significant. Among them , the detection rates of Mycobacterium tuberculosis , Mycoplasma pneumoniae , Haemophilus influenzae , Strepto⁃ coccus pneumoniae , Streptococcus constellation , Staphylococcus aureus and Aspergillus fumigatus were significantly higher than those of conventional tests , and the difference was statistically significant (P < 0. 05) . Subgroup analy- sis showed that the proportion of males , hospital stay , smoking prevalence and average age in the underlying dis- ease group were higher than those in the non-underlying disease group , and the difference was statistically signifi- cant (P < 0. 05) , while there were no significant differences in antibiotic use and endotracheal intubation rate be- tween the two groups. The most common pathogens detected by mNGS in the underlying disease group were Myco⁃ bacterium tuberculosis , Haemophilus influenzae , Streptococcus pneumoniae , Pseudomonas aeruginosa , human herpes⁃ virus type 4 and Aspergillus fumigatus , while the most common pathogens in the non-underlying disease group were Mycobacterium tuberculosis , Haemophilus influenzae , Streptococcus pneumoniae , Mycoplasma pneumoniae and Kleb⁃ siella pneumoniae. The positive rate of mNGS in the two groups was significantly higher than that of conventional tests , and the difference was statistically significant (P < 0. 05) , while the difference in the positive rate of mNGS between the two groups was not statistically significant. Conclusion mNGS has significant advantages over con- ventional tests of pathogen in lung infection , and is less affected by underlying diseases , which can provide an etio- logical basis for lung infection.

Objective:To compare the efficacy of transumbilical single-incision laparoscopic ovarian cystectomy versus multiport laparoscopic ovarian cystectomy in patients with ovarian cysts.Methods:Clinical data of 132 patients treated at Chengdu Third People's Hospital (January-December 2021) were retrospectively analyzed. Patients were divided into transumbilical single-incision laparoscopic ovarian cystectomy group ( n=70) and multiport laparoscopic ovarian cystectomy group ( n=62). Surgical parameters (operative time, blood loss, bowel recovery time, ambulation time, hospital stay), ovarian reserve function (LH, estradiol, FSH, AMH, inhibin B) both preoperatively and postoperatively, stress response (norepinephrine, cortisol), inflammatory markers (hs-CRP, IL-6, TNF-α), VAS pain scores at 24 h postoperative, scar satisfaction at 1 month and complication rates were compared between both group. Continuous data were expressed as xˉ± s, independent t-test was used on comparison between groups, while paired t-test was used on comparison before and after treatment, categorical data were expressed as n(%), χ2 test was used on comparison between groups. Results:The transumbilical single-incision laparoscopic ovarian cystectomy group had a shorter time on bowel recovery [(16.3±2.2) h vs. (20.8±3.0) h, t=9.87, P<0.001], ambulation [(12.9±2.6) h vs. (15.1±2.9) h, t=4.59, P<0.001] and hospitalization [(3.6±0.8) days vs. (5.3±1.1) days, t=9.65, P<0.001]. Both groups showed postoperative increases in LH, FSH, inhibin B, AMH [single-incision group: (13.2±3.4) U/L vs. (10.7±2.5) U/L, (9.4±1.6) U/L vs. (7.4±2.0) U/L, (66±13) μg/L vs. (44±8) μg/L, (5.2±1.2) μg/L vs. (1.7±0.4) μg/L, t values were 7.00、9.30、17.53、36.60, respectively, all P<0.001, multiport group: (14.1±3.6) U/L vs. (11.4±2.2) U/L, (9.8±1.8) U/L vs. (7.8±2.2) U/L, (62±15) μg/L vs. (43±8) μg/L, (4.9±1.1) μg/L vs. (1.7±0.4) μg/L, t values were 6.60、7.87、13.01、35.70, respectively, all P<0.001] and decreased estradiol [single-incision group: (304±29) pmol/L vs. (327±36) pmol/L, multiport group: (295±30) pmol/L vs. (329±37) pmol/L, t values were 5.92 and 7.99, respectively, both P<0.001], with no intergroup differences ( P>0.05). On postoperative day 1, the levels of serum NE, cortisol, hsCRP, IL-6, and TNF-α in both groups of patients were higher than those before surgery [single-incision group: (315±25) μg/L vs. (261±38) μg/L, (373±31) μg/L vs. (285±41) μg/L, (17.5±3.0) mg/L vs. (5.9±1.4) mg/L, (131±14) ng/L vs. (84±9) ng/L, (69±6) ng/L vs. (48±4) ng/L, t=14.34,20.45,44.11,34.19,35.14, respectively, all P<0.001; multiport group: (348±28) μg/L vs. (264±36) μg/L, (401±35) μg/L vs. (281±43) μg/L, (26.8±3.4) mg/L vs. (6.0±1.4) mg/L, (151±17) ng/L vs. (82±10) ng/L, (84±8) ng/L vs. (47±4) ng/L, t values were 20.67,25.74,68.24,40.24,48.56, respectively, all P<0.001], and the multiport group was higher than the single-incision group( t values were 7.12、4.94、16.89、7.60、11.54, respectively, all P<0.001). At 24 h postoperative, VAS scores was lower in transumbilical single-incision laparoscopic ovarian cystectomy group compared to multiport laparoscopic ovarian cystectomy group [(3.1±0.6) points vs. (4.3±0.9) points, t=4.41, P<0.001]. One month postoperative, scar satisfaction was higher in transumbilical single-incision laparoscopic ovarian cystectomy group compared to multiport laparoscopic ovarian cystectomy group [(21.0±1.5) points vs. (19.7±2.0) points, t=4.41, P<0.001].Difference on complications rate between groups was not statistically significant [14.3% (10/70) vs 21.0% (13/62), χ2=1.02, P=0.312]. Conclusion:Compared to multiport laparoscopy, transumbilical transumbilical single-incision laparoscopic ovarian cystectomy accelerates recovery, reduces pain, improves cosmetic outcomes, and minimizes surgical stress/inflammatory responses without compromising ovarian reserve or increasing complications.

Plant-derived bioactive peptides have become a research hotspot in the fields of food and medicine due to their high source safety,easy absorption and utilization by the human body,and potential edible and medicinal value.Bioactive peptides can be classified into antihypertensive,hypoglycemic,hypolipidemic,anticancer,antioxidant,antimicrobial,and anti-inflammatory peptides according to their functions.Among these,antihypertensive,hypoglycemic,and hypolipidemic peptides are collectively referred to as cardiovascular active peptides,which can be used for the treatment and prevention of cardiovascular diseases and have an important role in the development of modem biomedicine.This review focused on the preparation methods,separation,purification,and identification techniques of bioactive peptides,as well as their mechanisms of action and applications in regulating cardiovascular diseases,aiming to provide a reference for further development and application of plant-derived cardiovascular peptides.

Plant-derived bioactive peptides have become a research hotspot in the fields of food and medicine due to their high source safety,easy absorption and utilization by the human body,and potential edible and medicinal value.Bioactive peptides can be classified into antihypertensive,hypoglycemic,hypolipidemic,anticancer,antioxidant,antimicrobial,and anti-inflammatory peptides according to their functions.Among these,antihypertensive,hypoglycemic,and hypolipidemic peptides are collectively referred to as cardiovascular active peptides,which can be used for the treatment and prevention of cardiovascular diseases and have an important role in the development of modem biomedicine.This review focused on the preparation methods,separation,purification,and identification techniques of bioactive peptides,as well as their mechanisms of action and applications in regulating cardiovascular diseases,aiming to provide a reference for further development and application of plant-derived cardiovascular peptides.

Objective:To compare the efficacy of transumbilical single-incision laparoscopic ovarian cystectomy versus multiport laparoscopic ovarian cystectomy in patients with ovarian cysts.Methods:Clinical data of 132 patients treated at Chengdu Third People's Hospital (January-December 2021) were retrospectively analyzed. Patients were divided into transumbilical single-incision laparoscopic ovarian cystectomy group ( n=70) and multiport laparoscopic ovarian cystectomy group ( n=62). Surgical parameters (operative time, blood loss, bowel recovery time, ambulation time, hospital stay), ovarian reserve function (LH, estradiol, FSH, AMH, inhibin B) both preoperatively and postoperatively, stress response (norepinephrine, cortisol), inflammatory markers (hs-CRP, IL-6, TNF-α), VAS pain scores at 24 h postoperative, scar satisfaction at 1 month and complication rates were compared between both group. Continuous data were expressed as xˉ± s, independent t-test was used on comparison between groups, while paired t-test was used on comparison before and after treatment, categorical data were expressed as n(%), χ2 test was used on comparison between groups. Results:The transumbilical single-incision laparoscopic ovarian cystectomy group had a shorter time on bowel recovery [(16.3±2.2) h vs. (20.8±3.0) h, t=9.87, P<0.001], ambulation [(12.9±2.6) h vs. (15.1±2.9) h, t=4.59, P<0.001] and hospitalization [(3.6±0.8) days vs. (5.3±1.1) days, t=9.65, P<0.001]. Both groups showed postoperative increases in LH, FSH, inhibin B, AMH [single-incision group: (13.2±3.4) U/L vs. (10.7±2.5) U/L, (9.4±1.6) U/L vs. (7.4±2.0) U/L, (66±13) μg/L vs. (44±8) μg/L, (5.2±1.2) μg/L vs. (1.7±0.4) μg/L, t values were 7.00、9.30、17.53、36.60, respectively, all P<0.001, multiport group: (14.1±3.6) U/L vs. (11.4±2.2) U/L, (9.8±1.8) U/L vs. (7.8±2.2) U/L, (62±15) μg/L vs. (43±8) μg/L, (4.9±1.1) μg/L vs. (1.7±0.4) μg/L, t values were 6.60、7.87、13.01、35.70, respectively, all P<0.001] and decreased estradiol [single-incision group: (304±29) pmol/L vs. (327±36) pmol/L, multiport group: (295±30) pmol/L vs. (329±37) pmol/L, t values were 5.92 and 7.99, respectively, both P<0.001], with no intergroup differences ( P>0.05). On postoperative day 1, the levels of serum NE, cortisol, hsCRP, IL-6, and TNF-α in both groups of patients were higher than those before surgery [single-incision group: (315±25) μg/L vs. (261±38) μg/L, (373±31) μg/L vs. (285±41) μg/L, (17.5±3.0) mg/L vs. (5.9±1.4) mg/L, (131±14) ng/L vs. (84±9) ng/L, (69±6) ng/L vs. (48±4) ng/L, t=14.34,20.45,44.11,34.19,35.14, respectively, all P<0.001; multiport group: (348±28) μg/L vs. (264±36) μg/L, (401±35) μg/L vs. (281±43) μg/L, (26.8±3.4) mg/L vs. (6.0±1.4) mg/L, (151±17) ng/L vs. (82±10) ng/L, (84±8) ng/L vs. (47±4) ng/L, t values were 20.67,25.74,68.24,40.24,48.56, respectively, all P<0.001], and the multiport group was higher than the single-incision group( t values were 7.12、4.94、16.89、7.60、11.54, respectively, all P<0.001). At 24 h postoperative, VAS scores was lower in transumbilical single-incision laparoscopic ovarian cystectomy group compared to multiport laparoscopic ovarian cystectomy group [(3.1±0.6) points vs. (4.3±0.9) points, t=4.41, P<0.001]. One month postoperative, scar satisfaction was higher in transumbilical single-incision laparoscopic ovarian cystectomy group compared to multiport laparoscopic ovarian cystectomy group [(21.0±1.5) points vs. (19.7±2.0) points, t=4.41, P<0.001].Difference on complications rate between groups was not statistically significant [14.3% (10/70) vs 21.0% (13/62), χ2=1.02, P=0.312]. Conclusion:Compared to multiport laparoscopy, transumbilical transumbilical single-incision laparoscopic ovarian cystectomy accelerates recovery, reduces pain, improves cosmetic outcomes, and minimizes surgical stress/inflammatory responses without compromising ovarian reserve or increasing complications.

Humanistic caring for patients in the operating room refers to providing the whole process of caring medical services for patients in the operating room. In order to standardize humanistic caring services for patients in the operating room of medical institutions, improve the comprehensive service level of the operating room, and enhance the surgical experience of patients, the Chinese Association for Life Care released the group standard " Humanistic Caring Management Standards for Patients in the Operating Room" in December 2023. This article interpreted the basic requirements for humanistic caring of patients in the operating room, the environment and facilities for humanistic caring, the procedures and measures for humanistic caring, and the quality management framework, aiming to assist administrators and clinical practitioners across various levels of medical institutions in accurately understanding and effectively implementing the standard, and to provide essential textual reference and practical guidance for promoting the application of the standard.