Objective To explore adhering to 24-hour movement guidelines of disabled athletes,and its correlation to psychological resilience and subjective well-being.Methods A convenience sampling method was used to survey 192 disabled athletes from 17 provinces in China,to collect their demo-graphic information,compliance with 24-hour movement guidelines,psychological resilience and subjec-tive well-being.Ultimately,180 valid samples were obtained.Generalized linear models were used to analyze the relationship of meeting 24-hour movement guidelines to psychological resilience and subjec-tive well-being,and the roles of gender and type of disability were explored.Results On the average,disabled athletes spent 3.68 h on physical activities every week,but 4.89 h and 8 h on sedentary be-havior and sleep every day.The proportions of meeting 3 guidelines,2,and 1 were 26.1%,47.8%and 22.2%,respectively.Moreover,there was a dose-response relationship of the number of guide-lines met to psychological resilience(B=5.184,P<0.001)and subjective well-being(B=2.806,P<0.01).In other words,disabled athletes meeting more guidelines exhibited higher levels of psychological resil-ience(B=8.407,P<0.001)and subjective well-being(B=6.122,P<0.001).Conclusion Meeting 24-hour movement guidelines is of positive relation to psychological resilience and subjective well-being.There-fore,it is recommended to promote adherence to 24-hour movement guidelines among disabled ath-letes to promote their psychological health.

Objective To explore adhering to 24-hour movement guidelines of disabled athletes,and its correlation to psychological resilience and subjective well-being.Methods A convenience sampling method was used to survey 192 disabled athletes from 17 provinces in China,to collect their demo-graphic information,compliance with 24-hour movement guidelines,psychological resilience and subjec-tive well-being.Ultimately,180 valid samples were obtained.Generalized linear models were used to analyze the relationship of meeting 24-hour movement guidelines to psychological resilience and subjec-tive well-being,and the roles of gender and type of disability were explored.Results On the average,disabled athletes spent 3.68 h on physical activities every week,but 4.89 h and 8 h on sedentary be-havior and sleep every day.The proportions of meeting 3 guidelines,2,and 1 were 26.1%,47.8%and 22.2%,respectively.Moreover,there was a dose-response relationship of the number of guide-lines met to psychological resilience(B=5.184,P<0.001)and subjective well-being(B=2.806,P<0.01).In other words,disabled athletes meeting more guidelines exhibited higher levels of psychological resil-ience(B=8.407,P<0.001)and subjective well-being(B=6.122,P<0.001).Conclusion Meeting 24-hour movement guidelines is of positive relation to psychological resilience and subjective well-being.There-fore,it is recommended to promote adherence to 24-hour movement guidelines among disabled ath-letes to promote their psychological health.

Objective To construct and evaluate a novel PAMAM dendrimer vector-DNA vaccine for schistosomiasis japonica.Methods Lysine was used to modify 4.0G PAMAM.and the modified product PAMAM-Lys was synthesized.Agarose gel electrophoresis was used to confirm the composite ratio of plasmid DNA and dendrimer.Micrestructure of the compound was observed by using transmission electronic microscopy,and the stability was analyzed by using electrophoresis.The viability of the cells transfected with dendrimers was evaluated by using a MTT technique in vitro.Fiftyty mice were immunized with purified plasmid pJW4303,pJW4303-Sj23 dendrimer PAMAM-Lys and compound PAMAM-Lys/pJW4303-Sj23,respectively.The specific antibodies of the mice in each group were detected to access the immunoreactivity.Results The agarese gel electrophoresis showed that when the charge ratio of the dendrimer vector and DNA was between 2 and 4,the positive and negative charges could be counteraeted completely,and the compound was blocked completely by DNA electrophoresis.The obscrvation results with transmission electronic microscopy showed that the composition of dendrimer vector and DNA caused shrink of DNA structure.Dendrimer-DNA compound had a good stability.MTT showed the modified dendrimer vector and DNA compound system produced a lower cell toxicity on 293T cell than the unmodified Ones.Thk levels of specific antibodies of the mice immunized with PAMAM-Lys/pJW4303Sj23 were significantly higher than those of the mice immunized with naked DNA vaccine(P<0.05).Conclusions The lysinemodified PAMAM-lys is an excellent vector,and has an appropriate biocompatibility.Lysine-modification can reduce the cell toxicity of PAMAM dendrimer significantly.PAMAM-Lys can enhance the immunoreactivity of DNA vacmine which merits further application in schistosomiasis DNA vaccine.