Objective Exploring the relationship between the cognitive function level of elderly patients with Parkinson's disease (PD) and malnutrition. Methods A retrospective analysis was conducted on 456 patients with PD admitted to the hospital between April 2021 and April 2025. The nutritional status and cognitive function of patients were evaluated by Mini Nutrition Assessment Short Form (MNA-SF) and Montreal Cognitive Assessment (MoCA). Pearson correlation analysis explored the correlation between MNA-SF score and MoCA total score. Based on the occurrence status of cognitive dysfunction, the patients were classified into cognitive dysfunction group (264 cases) and normal cognitive function group (192 cases). Multivariate logistic regression was utilized to analyze the independent relevant factors of cognitive dysfunction. Results Among the 413 PD patients in this study, the MNA-SF score and total MoCA score were (9.35±2.16) points and (25.26±2.34) points. Pearson correlation analysis indicated that MNA-SF score was positively associated with MoCA total score (r=0.615, P<0.001). Compared with patients in the normal cognitive function group, the patients in the cognitive dysfunction group were older, had shorter years of education, longer PD course, higher proportion of cerebrovascular lesion and lower MNA-SF score (P<0.05). Logistic regression analysis revealed that age, PD course, cerebrovascular lesion and decreased MNA-SF score were independent relevant factors of cognitive dysfunction in elderly PD patients (P<0.05). Conclusion Malnutrition in elderly patients with Parkinson's disease is significantly associated with cognitive impairment, and as an independent factor, it can increase the risk of cognitive dysfunction in these patients.

Objective:To explore the application effect of Internet+ stratified teaching based on ClassIn platform in standardized training for neurology nurses.Methods:A total of 80 nurses who underwent standardized training between July 2022 and January 2024 were randomly divided into control group (traditional teaching) and observation group (Internet+ stratified teaching), with 40 participants in each group. In the observation group, 3D stratification was performed using the ClassIn platform. Specifically, layer A (extension group) received interdisciplinary PBL and VR advanced training (e.g., cerebrovascular intervention simulation), layer B (standard group) received personalized training package generated by an intelligent diagnosis system, and layer C (basic group) received stepped coding micro-class teaching. The control group received centralized lectures plus simulated man exercise. The differences in theoretical assessments, operational skills, clinical practice abilities, and comprehensive quality between the two groups were compared. SPSS 27.0 was used for t test and χ2 test. Results:The total theoretical assessment score of nurses in the observation group was higher than that in the control group, particularly in case analysis [neurology case analysis: (8.42±0.24) vs. (5.80±1.20)]. In operational skill assessment, the scores of GCS [(91.83±5.01) vs. (72.42±9.31)] and CPR [(93.41±4.90) vs. (73.60±9.12)] in the observation group were higher than those in the control group. In terms of clinical practice, nurses in the observation group demonstrated superior performance in condition observation ability [(8.50±1.10) vs. (5.80±1.70)] and health education [(9.20±0.70) vs. (6.50±1.30)]. In terms of the quality of humanistic care, the observation group outperformed the control group in complete rate of interpretation before operation [(8.61±0.54) vs. (6.67±1.12)], response rate of non-verbal demand [(8.43±0.37) vs. (6.15±1.02)], and rate of family members mastering nursing skills [(8.35±0.41) vs. (6.31±1.27)]. The comprehensive quality evaluation showed that team cooperation [(4.90±0.30) vs. (3.50±1.10)] and emergency response ability [(4.50±0.60) vs. (3.20±1.20)] were significantly improved in the observation group.Conclusions:The model of Internet+ stratified teaching based on ClassIn platform can effectively improve theoretical knowledge, operational standardization, and clinical comprehensive ability of neurology nurses. This approach emphasizes humanistic care in nursing. The intelligent stratification and dynamic regulation mechanism provide new ideas for nursing training.

Objective:To explore the application effect of Internet+ stratified teaching based on ClassIn platform in standardized training for neurology nurses.Methods:A total of 80 nurses who underwent standardized training between July 2022 and January 2024 were randomly divided into control group (traditional teaching) and observation group (Internet+ stratified teaching), with 40 participants in each group. In the observation group, 3D stratification was performed using the ClassIn platform. Specifically, layer A (extension group) received interdisciplinary PBL and VR advanced training (e.g., cerebrovascular intervention simulation), layer B (standard group) received personalized training package generated by an intelligent diagnosis system, and layer C (basic group) received stepped coding micro-class teaching. The control group received centralized lectures plus simulated man exercise. The differences in theoretical assessments, operational skills, clinical practice abilities, and comprehensive quality between the two groups were compared. SPSS 27.0 was used for t test and χ2 test. Results:The total theoretical assessment score of nurses in the observation group was higher than that in the control group, particularly in case analysis [neurology case analysis: (8.42±0.24) vs. (5.80±1.20)]. In operational skill assessment, the scores of GCS [(91.83±5.01) vs. (72.42±9.31)] and CPR [(93.41±4.90) vs. (73.60±9.12)] in the observation group were higher than those in the control group. In terms of clinical practice, nurses in the observation group demonstrated superior performance in condition observation ability [(8.50±1.10) vs. (5.80±1.70)] and health education [(9.20±0.70) vs. (6.50±1.30)]. In terms of the quality of humanistic care, the observation group outperformed the control group in complete rate of interpretation before operation [(8.61±0.54) vs. (6.67±1.12)], response rate of non-verbal demand [(8.43±0.37) vs. (6.15±1.02)], and rate of family members mastering nursing skills [(8.35±0.41) vs. (6.31±1.27)]. The comprehensive quality evaluation showed that team cooperation [(4.90±0.30) vs. (3.50±1.10)] and emergency response ability [(4.50±0.60) vs. (3.20±1.20)] were significantly improved in the observation group.Conclusions:The model of Internet+ stratified teaching based on ClassIn platform can effectively improve theoretical knowledge, operational standardization, and clinical comprehensive ability of neurology nurses. This approach emphasizes humanistic care in nursing. The intelligent stratification and dynamic regulation mechanism provide new ideas for nursing training.

Objective To explore the diagnosis,treatment and prognosis of neuroblastoma in children. Methods The clinical data of 36 children With neuroblastoma admitted to Department of Pediatric Surgery,Henan Pro﹣vincial People＇s Hospital betWeen August 2013 and August 2018,Were retrospectively analyzed. The patients included 22 males and 14 females With the age of 1 month－13 years old[( 20. 0 ± 18. 5)months]. TWenty－one cases of neu﹣roblastoma originated from the adrenal glands,the other 15 cases from the sympathetic ganglion( including 7 cases in retroperitoneum,6 cases in postmediastinum,and 2 cases in neck). Fifteen patients in loW risk and intermediate risk groups underWent primary surgery before multiple chemotherapy,While other 21 cases in high risk groups underWent chemotherapy until the mass could be removed completely,then continuely underWent multiple chemotherapy. Results Of the 36 patients,23 cases(63. 9%)had a radical resection,13 patients underWent palliative resection and 2 pa﹣tients Were treated With vascular repair during surgery(abdominal aorta in 1 patient,inferior vena cava in 1 patient). TWenty－seven patients had effective in formation and 16 patients survived,during folloW－up,12 patients belonged to stage Ⅰ－Ⅲ disease,2 patients stage Ⅳ disease and 2 patients stage Ⅳs disease,respectively. Four cases underWent re－operation due to disease recurrence,then they underWent chemothearopy,and 3 patients Were survival With tumor. Conclusions Neuroblastoma is a highly malignant pediatric cancer accompanied With a high rate of metastasis on ini﹣tial diagnosis. Radical resection is an effective therapeutic strategy for improving the survival rate. If radical resection is impractical,palliative resection combined With chemotherapy can significantly prolong patients＇ survival time. And a better prognosis is achieved if it can be early diagnosed and treated timely.

Objective:To evaluate the effects of Sander Ⅱ appliance in the orthodontic treatment of adolescent ClassⅡmalocclusion. Methods:15 cases (6 male and 9 female)of adolescent ClassⅡ malocclusion with mandibular retrution were treated with Sander Ⅱappliance.Pre-treatment and post-treatment Lateral cephalogram measurements were traced and analyzed in terms of 28 indicators. SPSS 19.0 software package was used for paired t test.Results:After treatment,15 patients achieved remarkable improvement in the maxillofacial profile and normal overjet of teeth.The cephalometric analysis showed that ANB,OJ,H°and U1-E decreased(P <0. 05),SNB,B-OLP,Pg-OLP(mm),Ii-OLP,Ms-OLP(mm),Mi-OLP(mm),N-Me,ANS-Me,S-Go,Go-Gn,N'-Pg'/FH,Cm-Sn-Ls and Pg-Pg'increased(P <0.05).There was no significant change in SN-MP,SN-OL and Y-axis before and after treatment. Conclusion:Sander Ⅱ appliance is effective in the treatment of adolescent early Class Ⅱmalocclusion.

Objective To evaluate the characteristics of brachyfacial types in adolescents with class Ⅱ~1 division 1 malocclusions by comparing with normal patterns.Methods 32 parameters were evaluated in pretreatment lateral cephalograms of 60 brachyfacial type adolescents,including 30 boys and 30 girls,aged from 11 to 13 years,with class Ⅱ~1 division 1 malocclusions(distal moral relationship,ANB＞5°,Go-Gn-SN＞28°).Results There was a remarkable increase in SNA,ANB,upper incisor inclination (U1-NA),U1-L1/N-Me and Me-L1/N-Me,so was lip protrusion of soft tissue facial profile(NB-Pg'-Ls);the lower sulcus depth(Si-Pg'-Ls)and the lower lip thickness(Li-L1).Mandibular plane(Go-Gn-SN),OP-MP,OP-FH,U1-L1,anterior lower facial height(ANS-Me),anterior lower facial height/anterior facial height(ANS-Me/N-Me),anterior lower facial height/posterior lower facial height (ALFH/PLFH)and mandibular length(B'-J')were significantly decreased.Conslusions Brachyfacial type in adolescents with class Ⅱ'division 1 malocclusions is characterized by decreased anterior lower facial height,increased posterior lower facial height,clockwise rotation of the palatal plane,anticlockwise rotation of the occlusion plane and mandibular plane,deepen overbite,soft tissue protrusive profiles with upper lip protrusion,crimpled lower lip and retracted chin.

A recombinant strain Escherichia coli DH5alpha(pMD19-glnA) including Bacillus subtilis glnA gene was constructed. Capillary electrophoresis and nuclear magnetic resonance were used to determine qualitatively the product of transformation by recombinant strain, and the relative level of mRNA expression of glnA was also determined by fluorescence quantitative RT-PCR. Subsequently, SDS-PAGE (polyacrylamide gel electrophoresis) was used to analysis the relative level of protein. Surprisingly, there was no increase of glutamine production in this recombinant strain, but an obvious increase in the GABA (gamma-aminobutyric acid ) production. It was showed in the experiment that protein expression of the glutamine synthetase did not increase, although glnA gene can be transcribed normally in this recombined strain. The phenomenon of exogenous glnA gene interfering metabolism of Escherichia coli was worthy of further study.
Bacillus subtilis ; genetics ; Bacterial Proteins ; genetics ; metabolism ; Escherichia coli ; genetics ; metabolism ; Glutamate-Ammonia Ligase ; genetics ; metabolism ; RNA, Messenger ; genetics ; metabolism ; Recombination, Genetic ; gamma-Aminobutyric Acid ; biosynthesis