The rapid screening of bioactive constituents within traditional Chinese medicine (TCM) presents a significant challenge to researchers. Prevailing strategies for the screening of active components in TCM often overlook trace components owing to their concealment by more abundant constituents. To address this limitation, a fishing strategy based on offline two-dimensional liquid chromatography (2D-LC) combined with surface plasmon resonance (SPR) was utilized to screen bioactive trace components targeting peroxiredoxin 3 (PRDX3), using Uncaria alkaloids (UAs) as a case study. Initially, an orthogonal preparative offline 2D-LC system combining a positively charged C₁₈ column and a conventional C₁₈ column under disparate mobile phase conditions was constructed. To fully reveal the trace alkaloids, 13 2D fractions of UAs were prepared, and their components were characterized using mass spectrometry (MS). Subsequently, employing PRDX3 as the targeting protein, a SPR-based screening approach was established and rigorously validated with geissoschizine methyl ether (GSM) serving as a positive control for binding. Employing this refined strategy, 29 candidate binding alkaloids were fished from the 13 2D fractions. Notably, combining offline 2D-LC with SPR increased the yield of candidate binding components from 10 to 29 when compared to SPR-based screening alone. Subsequent binding affinity assays confirmed that PRDX3 was a direct binding target for the 12 fished alkaloids, with isovallesiachotamine (IV), corynoxeine N-oxide (CO-N), and cadambine (CAD) demonstrating the highest affinity for PRDX3. Their interactions were further validated through molecular docking analysis. Subsequent intracellular H₂O₂ measurement assays and transfection experiments confirmed that these three trace alkaloids enhanced PRDX3-mediated H₂O₂ clearance. In conclusion, this study introduced an innovative strategy for the identification of active trace components in TCM. This approach holds promise for accelerating research on medicinal components within this field.

Objective:To carry out a scoping review on the current status and influencing factors of self-advocacy among cancer patients, so as to provide a basis for future intervention research.Methods:Research on cancer patients' self-advocacy was electronically retrieved on PubMed, Embase, CINAHL, Web of Science, Scopus, China National Knowledge Infrastructure, WanFang data, VIP, and China Biology Medicine disc. The search period was from database establishment to October 31, 2023. The included literature was summarized and analyzed.Results:A total of 17 articles were included. The influencing factors of cancer patients' self-advocacy mainly involved four aspects of general demographic factors, disease factors, social factors, and psychological factors.Conclusions:The influencing factors of cancer patients' self-advocacy are multifaceted. We should continue to explore the controversial influencing factors, conduct research on the self-advocacy of male cancer patients, and develop reasonable and effective intervention measures to enhance the self-advocacy level of cancer patients.