AIM:To investigate the impact of platycodin D(PD)on the viability,migration,invasion,apop-tosis and cell cycle of osteosarcoma cells in vitro,along with its underlying mechanisms.METHODS:Human osteosarco-ma cells MG63 and U2OS were divided into control group(0 μmol/L)and PD treatment group(6.25,12.5,25,50 and 100 μmol/L,respectively).Human osteosarcoma cells MG63 and U2OS were categorized into control groups(0 μmol/L PD)and PD treatment groups(6.25,12.5,25,50 and 100 μmol/L).The CCK-8 assay determined cell viability and identified effective treatment concentrations.MG63(15 μmol/L PD)and U2OS(25 μmol/L PD)were specifically ana-lyzed.Cell scratch and Transwell assays assessed migration and invasion.Hoechst 33342 staining examined nuclear mor-phological changes.Flow cytometry analyzed cell cycle distribution and apoptosis rate.Western blot measured protein ex-pression levels:cleaved caspase-3,cleaved PARP,c-Jun N-terminal kinase(JNK),p-JNK,B-cell lymphoma-2(Bcl-2),Bcl-2-ssociated X protein(BAX),matrix metalloproteinase 2(MMP-2),MMP-9,cyclin-dependent kinase 4(CDK4),cyclin D1,CDK1,cyclin B1,extracellular signal-regulated kinase(ERK)and p-ERK.Proteome sequencing of MG63 cells was performed.RESULTS:PD treatment significantly decreased cell survival,scratch healing rate,and invasive cell numbers,while increasing apoptosis rates(P<0.05).Morphological changes such as nuclear hyperchroma-tism and fragmentation were observed in PD-treated cells.PD induced G2/M phase arrest in MG63 and G0/G1 phase arrest in U2OS cells.PD treatment upregulated BAX,cleaved caspase-3,cleaved PARP,and p-JNK/JNK,while downregulat-ing Bcl-2,MMP-2,MMP-9,CDK4,cyclin D1,CDK1,cyclin B1,and p-ERK/ERK(P<0.05).Proteome sequencing re-vealed PD's involvement in cell division,cell cycle regulation,focal adhesion,apoptosis,and the MAPK signaling path-way.CONCLUSION:PD inhibits cell viability,migration,and invasion of osteosarcoma cells in vitro,while promoting apoptosis and inducing cell cycle arrest.These effects are likely mediated through modulation of the MAPK signaling path-way.

Objective:To explore influencing factors of the occurrence of fear of disease progression (FoP) in stroke patients and to construct a risk prediction nomogram model.Methods:A total of 201 stroke patients of the First Affiliated Hospital of Qiqihar Medical University and the Third Affiliated Hospital of Qiqihar Medical University from July to December 2023 were selected as the survey objects by the convenient sampling method. Baseline data questionnaire, Fear of Progression Questionnaire-Short Form (FoP-Q-SF), Chinese version of Perceived Stress Scale (CPSS), Social Support Rating Scale (SSRS) and Fatigue Severity Scale (FSS) were used to investigate of patients FoP occurrence, stress perception, social support level and fatigue level of stroke patients. The influencing factors of FoP occurrence in stroke patients were explored and R 4.3.2 was used to construct a risk prediction nomogram model for FoP occurrence in stroke patients. The performance of the model was evaluated using receiver operating characteristic curve, calibration curve, and clinical decision curve from the perspectives of discrimination, calibration and clinical practicality.Results:In this study, a total of 201 questionnaires were collected, 199 were valid, and the effective rate was 99%. The FOP-Q-SF score of 199 patients was (29.64±9.50), of which 71 patients (35.7%) developed FoP. Educational level, complications, self-care ability, social support, stress perception and fatigue were the influencing factors of FoP in stroke patients ( P<0.05). A risk prediction nomogram model for FoP in stroke patients was constructed based on the results of binary Logistic regression analysis. The Hosmer-Lemeshow results showed that the nomogram model fitted well (χ 2=10.466, P=0.234). The area under ROC curve was 0.912 (95% CI: 0.871-0.952). The clinical decision curve showed that when the threshold probability ranged from 4% to 99%, choosing this model to predict the risk of FoP could benefit stroke clinically. Conclusions:Educational level, complications, self-care ability, social support, stress perception and fatigue are the influencing factors for fear of disease progression in stroke patients. The risk nomogram model based on multiple evaluation indexes has certain clinical value.

Objective A comparison of two method of establishing pressure ulcer rat models to determine which is the most suitable for experimental use.Methods 18 male SD rats were randomly divided into control(n=6),model A(n=6)and model B(n=6)groups.In the control group,iodophor treatment was given after hair removal at the simulated modeling site.In model group A,longitudinal compression was performed by simple deep-tissue foreign body implantation.In model group B,transverse compression was performed via the magnet compression method.The times required to complete the process and for each stage of pressure ulcer model establishment in each group were recorded.The general condition of the rats was observed,and the modeling rate,mortality rate,and infection rate were compared.Results By naked eye,we observed that the model A and model B groups gradually developed redness and swelling,ulceration,bleeding,exudation,and necrosis.Comparison of the whole time to produce pressure uler between model A and model B groups:the difference between the two groups was statitically significant(P＜0.05).Comparison of the time to produce pressure injury between Model A and Model B:The difference between the two groups at stage Ⅰ was not statistically significant(P＞0.05);the difference between the two groups at stage Ⅱ was statistically significant(P＜0 05);the difference between the two groups at stage Ⅲ was statistically significant(P＜0 05);the difference between the two groups at stage Ⅳ was statistically significant(P＜0 05).The mental and sports scores of the rats in the control group were significantly different from those in the model A and model B groups(P＜0.05).The general state of rats in the model group A was significantly different from that in the model B group,and coat color was dimer and activity decreased in the model group A.The modelling rate of rats in both model A and model B groups was 100％.The mortality and infection rates of the model group A were higher than those of the model group B,which were 33.34％and 16.70％,respectively.Conclusions Successful preparation of a four-stage model of pressure ulers in both modalities.The two method have both commonalities and distinct characteristics.The magnet compression method required less time,the rats were generally in good condition,and the mortality and infection rates were low;thus it is suitable for short-term intervention research.The simple deep-tissue foreign body implantation method took longer,required rats to have a certain level of tolerance,had high infection and mortality rates,and is more suitable for use for long-term observations of pressure ulcers.

Objective:To investigate the incidence characteristics and influencing factors of non-alcoholic fatty liver disease (NAFLD) in rural Uyghur ethnic group residents in Xinjiang Production and Construction Corps and to provide scientific evidence for early identification and prevention of NAFLD for residents.Methods:A total of 10 158 participants were included from the Xinjiang Uygur ethnic group population cohort. A prospective cohort study and Cox proportional hazards regression model analysis were used to explore the influencing factors and clustering of NAFLD, and the dose-response relationship between related biochemical indicators and the risk of NAFLD was studied using a restricted cubic spline.Results:The cumulative incidence rate of NAFLD was 6.9%, and the incidence density of NAFLD was 12.06/1 000 person-years. The incidence density of NAFLD in females was higher than in males (14.72/1 000 person-years vs. 9.17/1 000 person-years, P<0.001). The incidence density of NAFLD gradually increased with age in the total population, both men and women (all P<0.001). In the general population, an education level of junior high school or above was a protective factor for NAFLD, while older age, divorce, widowhood, overweight, obesity, hypertension, increased glomerular filtration rate, decreased HDL-C, increased LDL-C, and increased ALT were risk factors for NAFLD. Estimated glomerular filtration rate (eGFR), HDL-C, LDL-C, and ALT were non-linearly correlated with the incidence of NAFLD, and there was a significant dose-response relationship between them. Only 19.1% of residents had no NAFLD risk factors; over 80.9% had ≥1 NAFLD risk factors. The risk of NAFLD increased with the number of risk factors. Conclusions:The incidence of NAFLD in rural Uygur ethnic group residents in Xinjiang Production and Construction Corps was relatively low, but most residents had one or more risk factors for NAFLD. Prevention and control of NAFLD in this population cannot be ignored. In addition, people of older age, divorced or widowed, low education level, overweight or obese, hypertension, and abnormal eGFR, HDL-C, LDL-C, and ALT were the high-risk groups of NAFLD that need to be paid attention to in this population.

Objective To investigate and analyze the impact of three personalized surgical design schemes for SPT trans PRK on postoperative visual quality and higher-order aberrations in individuals with myopic astigma-tism,aiming to provide a foundation for more rational selection of personalized design schemes.Methods The 96 cases(96 eyes)with myopic astigmatism were divided into three groups based on three personalized design schemes and a conventional mode.Specifically,24 eyes were assigned to the personalized group 1,which focused on coma elimination;another 24 eyes belonged to personalized group 2,where the aim was to minimize spherical aberration elimination;and the remaining 24 eyes were further categorized into personalized group 3 based on a model that aimed at minimizing spherical aberration.Additionally,there were also 24 eyes in the control group treated using the conventional mode.The study compared and analyzed various parameters including best corrected visual acuity,spherical aberration,coma,total higher-order aberration of the anterior corneal surface,as well as differences in corneal ablation thickness between personalized and conventional schemes within the surgical design software.Results(1)The postoperative visual acuity of the personalized group was significantly superior to that of the control group(P＜0.05);(2)Among the personalized groups,Group 2 exhibited a reduced amount of cor-neal tissue ablation compared to other groups(P＜0.01);(3)Group 2 demonstrated lower values than the other groups after surgery(P＜0.05).(4)Coma:The control group showed a significantly higher level of coma com-pared to preoperative measurements(P＜0.01).No significant differences were observed between Groups 1,2,and 3 after surgery(P＞0.05).(5)Total higher-order aberrations:All groups experienced a significant increase in total higher-order aberrations following surgery(P＜0.01).Group 2 exhibited lower values than the other groups postoperatively(P＜0.05).Conclusion For myopic astigmatism,SPT trans PRK incorporates the personalized surgical scheme with a focus on minimizing spherical aberration elimination mode,resulting in enhanced optimiza-tion of postoperative high-order aberration and improved visual quality,while preserving corneal tissue.

Objective To investigate and analyze the impact of three personalized surgical design schemes for SPT trans PRK on postoperative visual quality and higher-order aberrations in individuals with myopic astigma-tism,aiming to provide a foundation for more rational selection of personalized design schemes.Methods The 96 cases(96 eyes)with myopic astigmatism were divided into three groups based on three personalized design schemes and a conventional mode.Specifically,24 eyes were assigned to the personalized group 1,which focused on coma elimination;another 24 eyes belonged to personalized group 2,where the aim was to minimize spherical aberration elimination;and the remaining 24 eyes were further categorized into personalized group 3 based on a model that aimed at minimizing spherical aberration.Additionally,there were also 24 eyes in the control group treated using the conventional mode.The study compared and analyzed various parameters including best corrected visual acuity,spherical aberration,coma,total higher-order aberration of the anterior corneal surface,as well as differences in corneal ablation thickness between personalized and conventional schemes within the surgical design software.Results(1)The postoperative visual acuity of the personalized group was significantly superior to that of the control group(P＜0.05);(2)Among the personalized groups,Group 2 exhibited a reduced amount of cor-neal tissue ablation compared to other groups(P＜0.01);(3)Group 2 demonstrated lower values than the other groups after surgery(P＜0.05).(4)Coma:The control group showed a significantly higher level of coma com-pared to preoperative measurements(P＜0.01).No significant differences were observed between Groups 1,2,and 3 after surgery(P＞0.05).(5)Total higher-order aberrations:All groups experienced a significant increase in total higher-order aberrations following surgery(P＜0.01).Group 2 exhibited lower values than the other groups postoperatively(P＜0.05).Conclusion For myopic astigmatism,SPT trans PRK incorporates the personalized surgical scheme with a focus on minimizing spherical aberration elimination mode,resulting in enhanced optimiza-tion of postoperative high-order aberration and improved visual quality,while preserving corneal tissue.

Objective:To investigate the association of cardiovascular diseases (CVD) with triglyceride glucose index (TyG) and TyG-related indicators in Uyghur populations of The Xinjiang Production and Construction Corps.Methods:Based on the cohort of the Uygur population of The Xinjiang Production and Construction Corps, 11 833 study subjects were included. The Kaplan-Meier method was used to estimate the cumulative incidence of CVD in each quartile of TyG and TyG-related indicators. Cox proportional hazards regression model was used to evaluate the relationship between TyG and CVD, TyG-related indicators and CVD. Framingham CVD risk score model (Framingham model) was used to evaluate whether the addition of TyG and TyG-related indicators could improve the predictive ability of the model. The potential mediating role of the TyG in the association between obesity and CVD was examined through mediation effect analysis.Results:The average age of the subjects was (37.00±13.67) years-old, and 51.0% were male. The median follow-up time was 5.67 years, with 1 288 CVD events. The cumulative incidence of CVD increased with the increase of TyG and TyG-related indicators quartiles, and compared with the Q1 group, the risk of CVD in the Q4 group of TyG, TyG-BMI, and TyG-WHtR increased by 20% ( HR=1.20, 95% CI: 1.01-1.42), 77% ( HR=1.77, 95% CI: 1.46-2.16) and 68% ( HR=1.68, 95% CI: 1.36-2.09), respectively. After adding TyG, TyG-BMI, and TyG-WHtR to the Framingham model, respectively, the model's area under the curve, net reclassification improvement, and integrated discrimination improvement were improved. In the association between BMI, WHtR, and CVD, the proportion of mediating effects mediated by the TyG index was 10.55% and 11.50%. Conclusions:Elevated levels of TyG and TyG-related indicators were strongly associated with the risk of CVD in the Uyghur population of The Xinjiang Production and Construction Corps, with TyG-BMI being the most closely correlated with CVD. Early monitoring of TyG-BMI helps identify high-risk groups of CVD.

Objective:To analyze whether triglyceride-glucose (TyG) index is associated with increased risk of cardiovascular diseases (CVD) and the value of TyG index in predicting CVD risk among Kazakh and Uighur population of Xinjiang.Methods:In this study, 5 375 Kazakh and Uygur people of Xinyuan county and Jiashi county were selected as the research objects. Subjects were divided into four groups based on the quartile of the TyG index level. Cox regression model was used to analyze the association between TyG index with the risk of CVD. The dose-response relationship between TyG index and CVD risk was described by restricted cubic splines. The area under the receiver operating characteristic curve, net reclassification improvement (NRI), and integrated discrimination improvement (IDI) were used to estimate the value of TyG index for predicting CVD. Mediating effect analysis was conducted to analyze the mediating effect of TyG index in the association between body mass index and CVD.Results:The age of subjects was 41.06（30.11，53.00）years old, with 46.30%（2 489/5 375）was male. After multivariate adjustment, there was an increasing trend between the risk of CVD and the higher TyG index Ptrend<0.001, compared with subjects of TyG index in Q1, the HR (95% CI) of Q2, Q3, and Q4 groups was 1.53, 1.23 and 1.73, respectively. Restricted cubic splines showed that TyG index was the linearly associated with the risk of CVD. TyG index could improve the prediction ability of Framingham model for the risk of CVD (NRI=0.106, P=0.010; IDI=0.003, P=0.030). The mediating effect analysis showed that in the relationship between body mass index and CVD, the TyG index had a mediating effect ( P<0.001), and the ratio of mediating effect was 12.69%. Conclusion:TyG index is an independent predictor of CVD risk among kazakh and Uygur population in Xinjiang and has a good predictive value for the risk of CVD.

Objective:To analyze whether triglyceride-glucose (TyG) index is associated with increased risk of cardiovascular diseases (CVD) and the value of TyG index in predicting CVD risk among Kazakh and Uighur population of Xinjiang.Methods:In this study, 5 375 Kazakh and Uygur people of Xinyuan county and Jiashi county were selected as the research objects. Subjects were divided into four groups based on the quartile of the TyG index level. Cox regression model was used to analyze the association between TyG index with the risk of CVD. The dose-response relationship between TyG index and CVD risk was described by restricted cubic splines. The area under the receiver operating characteristic curve, net reclassification improvement (NRI), and integrated discrimination improvement (IDI) were used to estimate the value of TyG index for predicting CVD. Mediating effect analysis was conducted to analyze the mediating effect of TyG index in the association between body mass index and CVD.Results:The age of subjects was 41.06（30.11，53.00）years old, with 46.30%（2 489/5 375）was male. After multivariate adjustment, there was an increasing trend between the risk of CVD and the higher TyG index Ptrend<0.001, compared with subjects of TyG index in Q1, the HR (95% CI) of Q2, Q3, and Q4 groups was 1.53, 1.23 and 1.73, respectively. Restricted cubic splines showed that TyG index was the linearly associated with the risk of CVD. TyG index could improve the prediction ability of Framingham model for the risk of CVD (NRI=0.106, P=0.010; IDI=0.003, P=0.030). The mediating effect analysis showed that in the relationship between body mass index and CVD, the TyG index had a mediating effect ( P<0.001), and the ratio of mediating effect was 12.69%. Conclusion:TyG index is an independent predictor of CVD risk among kazakh and Uygur population in Xinjiang and has a good predictive value for the risk of CVD.

The integration of artificial intelligence into the medical field is developing rapidly and has achieved ground-breaking advances in the diagnosis, treatment, and efficacy evaluation of imaging medicine. This article reviews the research advances in artificial intelligence in imaging diagnosis of hepatocellular carcinoma and its performance in evaluating treatment outcome and predicting prognosis in combination with clinical features and looks forward to how artificial intelligence can be better used in the practice of hepatocellular carcinoma imaging in the era of growing clinical needs and rapid advances in diagnosis and treatment techniques.