OBJECTIVE To evaluate the efficacy and safety of amoxicillin combined with proton pump inhibitor （PPI） or potassium-competitive acid blocker （P-CAB） for Helicobacter pylori （Hp） eradication. METHODS Randomized controlled trial （RCTs） on amoxicillin combined with PPI or P-CAB for Hp eradication were retrieved from PubMed， Embase， the Cochrane Library， Web of Science， CNKI， Wanfang， and VIP data. The search time frame was from database inception to September 5， 2025. After literature screening， data extraction， and quality assessment， a network meta-analysis was performed using Stata 17.0 software. RESULTS A total of 12 RCTs involving 5 515 patients were included， encompassing 8 therapeutic regimens： PPI combined with high-dose amoxicillin for 14 days （TR1）， PPI combined with low-dose amoxicillin for 14 days （TR2）， P-CAB combined with high-dose amoxicillin for 7 days （TR3）， P-CAB combined with high-dose amoxicillin for 14 days （TR4）， P-CAB combined with high-dose amoxicillin for 10 days （TR5）， P-CAB combined with low-dose amoxicillin for 7 days （TR6）， P-CAB combined with low-dose amoxicillin for 14 days （TR7）， and P-CAB combined with low-dose amoxicillin for 10 days （TR8）. The network meta-analysis results showed that， in terms of intention-to-treat Hp eradication rates， the eradication rates of TR5 and TR4 were significantly higher than those of TR3， TR8， TR6 and TR1 （ P ＜0.05）. The surface under the cumulative ranking curve （SUCRA） values from highest to lowest were： TR4 （89.7%）＞TR5 （82.3%）＞TR7 （71.5%）＞ TR2 （48.6%）＞TR1 （43.9%）＞TR8 （28.7%）＞TR3 （22.7%）＞TR6 （12.6%）. Regarding safety， the incidence of adverse reactions in TR3 and TR5 was significantly lower than that in TR1 （ P ＜0.05）. The SUCRA values from highest to lowest were： TR1 （91.3%）＞TR4 （79.8%）＞TR5 （55.0%）＞TR7 （50.9%）＞TR8 （41.3%）＞TR2 （36.4%）＞TR3 （27.6%） ＞TR6 （17.7%）. CONCLUSIONS Although the regimen of P-CAB combined with high-dose amoxicillin for 14 days demonstrates the best efficacy， the combination of P-CAB with high-dose amoxicillin for 10 days exhibits a better balanced profile in terms of both efficacy and safety.

Neurofibromatosis type 1 (NF1) presents with a diverse range of symptoms that can affect the skin, bones, eyes, central nervous system, and other organs. This article reports the diagnosis and treatment process of a patient with NF1 complicated by bilateral severe-to-profound sensorineural hearing loss. Genetic testing revealed a heterozygous variant of NF1 NM_ 000267.3: c.4054_ 4058del(p.Ser1352LeufsTer20, supporting the diagnosis of NF1. After thorough evaluation, a right cochlear implantation was performed, yielding satisfactory postoperative results. This case suggests that NF1 patients may exhibit phenotypic heterogeneity and atypicality, providing a reference for clinicians in the diagnosis and treatment of such patients.

Sucrose non-fermenting 1-related protein kinase 1 (SnRK1) is one of the highly conserved Ca²⁺ non-dependent serine/threonine protein kinases, playing a crucial role in regulating the stress responses, growth, and development of plants. SnRK1 is a three-subunit complex, and it is involved in responding to the signaling transduction induced by low-energy/low-sugar conditions. SnRK1 responds biotic and abiotic stress conditions (such as salt, drought, low/high temperatures, and diseases) through phosphorylation of key metabolic enzymes and regulatory proteins, regulation of transcription, and interactions with other proteins. Furthermore, SnRK1 is not only involved in hormone signaling pathways mediated by abscisic acid (ABA), jasmonic acid (JA) and salicylic acid (SA), but also regulates plant autophagy by inhibiting the activity of target of rapamycin (TOR). In this review, we summarized the current results of research on the discovery, structure, and classification of plant SnRK1 and its roles in the stress responses, growth, and development of plants. Furthermore, this article proposes the directions of future research. This review provides good genetic resources and a theoretical basis for the genetic improvement and biological breeding for enhancing the stress tolerance of crops.
Stress, Physiological/physiology* ; Protein Serine-Threonine Kinases/metabolism* ; Plant Development/genetics* ; Signal Transduction ; Gene Expression Regulation, Plant ; Plant Proteins/physiology* ; Plants/metabolism* ; Arabidopsis Proteins/physiology* ; Plant Growth Regulators/metabolism*

Objective:To systematically evaluate and integrate qualitative studies on the disease experience and demands of psoriasis, and to provide reference for improving patients′ disease experience and formulating targeted nursing intervention strategies.Methods:Web of science, PubMed, Embase, Cochrane Library, Medline, China National Knowledge Infrastructure, Wanfang database, Weipu database and China Biology Medicine Disc were searched by computer from their inception to May 31, 2024 for the qualitative studies on experience of psoriasis patient experience. The literature quality was appraised by the evaluation criteria of the Evidence Based Healthcare Center at the Joanna Briggs Institute for qualitative research. Findings of included studies were synthesized using Meta-aggregation.Results:A total of 13 articles were included, and 48 research results were extracted, which were further formed into 9 categories, and finally summarized into the following 3 integrated results: disease infects life, and physical and mental difficulties were intertwined; response strategies varied, affecting disease outcome; multiple needs were not yet met, and multi-level support systems were urgently neededConclusions:Psoriasis patients suffer from the burden of symptoms and psychological pressure, medical personnel should pay attention to patients′ inner experience and nursing needs, provide multi-dimensional support to reduce disease distress, improve patients′ disease management level, and improve quality of life.

Objective:To systematically review the qualitative studies on the experience of self-empowerment in cancer patients, and provide evidence for better cancer care centered on patients.Methods:Computer searches were conducted in PubMed, Web of Science, Embase, Cochrane Library, Medline, China National Knowledge Infrastructure, Wanfang, and VIP, China Biomedical Literature Database for qualitative studies on the experience of self-empowerment in cancer patients from database inception to April 2, 2024. The quality of the literature was evaluated according to the Joanna Briggs Institute Evidence-Based Practice Unit's quality assessment criteria for qualitative studies. Meta-synthesis was conducted using a centralized integration method.Results:Five studies were included, yielding 17 research findings, which were categorized into 16 categories and 4 synthesis results: cancer patients′ cognition of self-empowerment; barriers to self-empowerment; diversity of self-empowerment strategies; and the role of external support.Conclusions:Cancer patients recognize the value of self-empowerment, but there are multiple factors that hinder patients from self-empowering. Healthcare providers should understand the self-empowerment needs of patients and provide evidence-based care centered on patients to promote better health outcomes for patients.

Objective:To systematically evaluate the qualitative studies on nurses' knowledge and practice experience of grief counseling, providing a reference for the implementation and development of grief counseling.Methods:Computer-based searches were conducted in PubMed, Web of Science, Embase, Cochrane Library, Medline, China National Knowledge Infrastructure, WanFang Data, VIP, and China Biology Medicine disc for qualitative studies on nurses' knowledge and practice experience of grief counseling, with the search limited to articles published until January 5, 2024. The quality of the literature was assessed using the quality evaluation criteria of the Evidence-Based Health Care Center of the Joanna Briggs Institute, Australia. A pooled integration approach was used to integrate the literature.Results:A total of seven studies were included, yielding 26 research findings, which were categorized into 10 categories, further integrated into four synthesis results, namely, nurses' perceptions of grief counseling, barriers to the implementation of grief counseling by nurses, strategies to promote nurses' implementation of grief counseling, and nurses' emotional experiences.Conclusions:The implementation of grief counseling faces challenges. Healthcare managers should understand nurses' knowledge and practice experience of grief counseling, provide relevant training, and strengthen organizational support to promote the practice and development of grief counseling.

Objective:To systematically evaluate and integrate qualitative studies on the disease experience and demands of psoriasis, and to provide reference for improving patients′ disease experience and formulating targeted nursing intervention strategies.Methods:Web of science, PubMed, Embase, Cochrane Library, Medline, China National Knowledge Infrastructure, Wanfang database, Weipu database and China Biology Medicine Disc were searched by computer from their inception to May 31, 2024 for the qualitative studies on experience of psoriasis patient experience. The literature quality was appraised by the evaluation criteria of the Evidence Based Healthcare Center at the Joanna Briggs Institute for qualitative research. Findings of included studies were synthesized using Meta-aggregation.Results:A total of 13 articles were included, and 48 research results were extracted, which were further formed into 9 categories, and finally summarized into the following 3 integrated results: disease infects life, and physical and mental difficulties were intertwined; response strategies varied, affecting disease outcome; multiple needs were not yet met, and multi-level support systems were urgently neededConclusions:Psoriasis patients suffer from the burden of symptoms and psychological pressure, medical personnel should pay attention to patients′ inner experience and nursing needs, provide multi-dimensional support to reduce disease distress, improve patients′ disease management level, and improve quality of life.

Objective:To systematically review the qualitative studies on the experience of self-empowerment in cancer patients, and provide evidence for better cancer care centered on patients.Methods:Computer searches were conducted in PubMed, Web of Science, Embase, Cochrane Library, Medline, China National Knowledge Infrastructure, Wanfang, and VIP, China Biomedical Literature Database for qualitative studies on the experience of self-empowerment in cancer patients from database inception to April 2, 2024. The quality of the literature was evaluated according to the Joanna Briggs Institute Evidence-Based Practice Unit's quality assessment criteria for qualitative studies. Meta-synthesis was conducted using a centralized integration method.Results:Five studies were included, yielding 17 research findings, which were categorized into 16 categories and 4 synthesis results: cancer patients′ cognition of self-empowerment; barriers to self-empowerment; diversity of self-empowerment strategies; and the role of external support.Conclusions:Cancer patients recognize the value of self-empowerment, but there are multiple factors that hinder patients from self-empowering. Healthcare providers should understand the self-empowerment needs of patients and provide evidence-based care centered on patients to promote better health outcomes for patients.

Objective:To systematically evaluate the qualitative studies on nurses' knowledge and practice experience of grief counseling, providing a reference for the implementation and development of grief counseling.Methods:Computer-based searches were conducted in PubMed, Web of Science, Embase, Cochrane Library, Medline, China National Knowledge Infrastructure, WanFang Data, VIP, and China Biology Medicine disc for qualitative studies on nurses' knowledge and practice experience of grief counseling, with the search limited to articles published until January 5, 2024. The quality of the literature was assessed using the quality evaluation criteria of the Evidence-Based Health Care Center of the Joanna Briggs Institute, Australia. A pooled integration approach was used to integrate the literature.Results:A total of seven studies were included, yielding 26 research findings, which were categorized into 10 categories, further integrated into four synthesis results, namely, nurses' perceptions of grief counseling, barriers to the implementation of grief counseling by nurses, strategies to promote nurses' implementation of grief counseling, and nurses' emotional experiences.Conclusions:The implementation of grief counseling faces challenges. Healthcare managers should understand nurses' knowledge and practice experience of grief counseling, provide relevant training, and strengthen organizational support to promote the practice and development of grief counseling.

Respiratory syncytial virus(RSV)is a major cause of severe lower respiratory disease and can infect all populations,posing a significant health threat to infants and the elderly.After more than 60 years of research,there are only 2 RSV vaccines and 2 preventive monoclonal antibodies be licensed,but the use of the population is severely limited,expensive and difficult to large-scale popularization.RSV vaccine development faces a number of obstacles,such as the lack of animal models,avoidance of RSV immunity,and short duration of immunity.Since the failure of formalin inactivated RSV vaccine trials,RSV vaccine development has become more cautious and difficult.This article briefly reviews the research status of RSV vaccines,and introduces several representative vaccines currently under development,in order to facilitate researchers to review the latest progress and contribute to promoting vaccine research.