Objective:To evaluate corrective effect of cone beam computed tomography(CBCT)in children with malocclusion during mixed dentition period.Methods:A total of 90 children with malocclusion during mixed dentition period who admitted to 3201th hospital between January and December 2023 were enrolled,and all of them underwent routine orthodontics.Before and after treatment,CBCT scan was performed to obtain oral and maxillofacial imaging data.According to measurement and analysis for imaging data,the corrective effect,the relation indicators between sagittal and vertical directions of jawbone,which included the constructed angle among the center point of sella turcica-nasion-superior alveolar seat(SNA),the constructed angle among the center point of sella turcica-nasion-inferior alveolar seat(SNB),the constructed angle among superior alveolar seat-nasion-inferior alveolar seat(ANB),the constructed angle between occlusal plane and sella-nasion plane(OP-SN),the angle between mandibular plane and extended line of occlusal plane(SN-MP),were assessed.In addition,the other related mandibular indicators included the angle(Y axis angle)between Y axis and orbitoauricular plane,the angle of nasion A point line-orbitale-pterygomaxillary fissure line(NA-Apo),gonion-pogonion length(Go-Pg),the distance values(Wits)between anterior nasal crest and orbitomeatal plane,and between posterior nasal crest and orbitomeatal plane also were assessed.Third,the soft tissue-related indicators,which included the distance(UL-EP)between upper lip protrusion point,nasal protrusion point and soft tissue line,and the distance(LL-EP)between lower lip protrusion point,nasal protrusion point and soft tissue line,and the nasal labial angle(NLA),the lower intersection angle between the line connecting the nasal columella point and the infranasal point,and the lower intersection angle between the line connecting the nasal columella point and upper lip protrusion point,and the angle(H)between H line and soft tissue surface angle,were assessed.Finally,the dentally relative indicators,which included incisor mandibular plane angle(IMPA angle),upper central incisor-sella nasion plane angle(U1-SN),upper central incisor-nasion appoint angle(U1-NA),lower central incisor-nasion B point angle(L1-NB),overbite and coverage,were also assessed.Results:In the 90 children with malocclusion during mixed dentition period after treatment,25 cases(27.78%)were cured,and 56 cases obtained effective treatment(62.22%)and 9 cases(10.00%)did not obtain effective treatment,and effective rate of correction was 90.00%.There was not significant change in SNB angle between before and after treatment,and the difference was not statistically significant(P>0.05).Compared with the indicators before treatment,OP-SN was decreased,while SN-MP,SNA and ANB were all increased after treatment,and the differences were statistically significant(t=6.402,6.135,3.753,50.965,P<0.05).There were not significant changes in Y-axis angle,Wits value,S-Go/N-Me and L1-NB angle between before and after treatment,and the differences were not statistically significant(P>0.05).Compared with the indicators before treatment,the NA-Apo and Go-Pg were increased after treatment,and the differences were statistically significant(t=46.885,7.575,P<0.05).Compared with the indicators before treatment,the NLA,H angle and UL-EP were increased,while LL-EP was decreased after treatment,and the differences were statistically significant(t=2.707,4.163,28.622,3.276,P<0.05).There was not significant change in L1-NB between before and after treatment,and the difference was not statistically significant(P>0.05).Compared with the indicators before treatment,the coverage,U1-SN and U1-NA were increased,while overbite and IMPA were decreased,and the differences were statistically significant(t=16.154,4.723,6.432,4.802,4.106,P<0.05).Conclusion:CBCT can clearly and accurately display three-dimensional morphology and changes of maxillofacial structure of oral cavity,which can provides comprehensive and reliable basis for the assessment for correction effect.Moreover,orthodontic treatment has positive significance in improving dentition occlusion relationship,guiding normal development of jawbone,and optimizing morphology of facial soft tissues,which can provide important reference for the formulation and optimization of the treatment scheme of clinical orthodontics.

Objective:To evaluate corrective effect of cone beam computed tomography(CBCT)in children with malocclusion during mixed dentition period.Methods:A total of 90 children with malocclusion during mixed dentition period who admitted to 3201th hospital between January and December 2023 were enrolled,and all of them underwent routine orthodontics.Before and after treatment,CBCT scan was performed to obtain oral and maxillofacial imaging data.According to measurement and analysis for imaging data,the corrective effect,the relation indicators between sagittal and vertical directions of jawbone,which included the constructed angle among the center point of sella turcica-nasion-superior alveolar seat(SNA),the constructed angle among the center point of sella turcica-nasion-inferior alveolar seat(SNB),the constructed angle among superior alveolar seat-nasion-inferior alveolar seat(ANB),the constructed angle between occlusal plane and sella-nasion plane(OP-SN),the angle between mandibular plane and extended line of occlusal plane(SN-MP),were assessed.In addition,the other related mandibular indicators included the angle(Y axis angle)between Y axis and orbitoauricular plane,the angle of nasion A point line-orbitale-pterygomaxillary fissure line(NA-Apo),gonion-pogonion length(Go-Pg),the distance values(Wits)between anterior nasal crest and orbitomeatal plane,and between posterior nasal crest and orbitomeatal plane also were assessed.Third,the soft tissue-related indicators,which included the distance(UL-EP)between upper lip protrusion point,nasal protrusion point and soft tissue line,and the distance(LL-EP)between lower lip protrusion point,nasal protrusion point and soft tissue line,and the nasal labial angle(NLA),the lower intersection angle between the line connecting the nasal columella point and the infranasal point,and the lower intersection angle between the line connecting the nasal columella point and upper lip protrusion point,and the angle(H)between H line and soft tissue surface angle,were assessed.Finally,the dentally relative indicators,which included incisor mandibular plane angle(IMPA angle),upper central incisor-sella nasion plane angle(U1-SN),upper central incisor-nasion appoint angle(U1-NA),lower central incisor-nasion B point angle(L1-NB),overbite and coverage,were also assessed.Results:In the 90 children with malocclusion during mixed dentition period after treatment,25 cases(27.78%)were cured,and 56 cases obtained effective treatment(62.22%)and 9 cases(10.00%)did not obtain effective treatment,and effective rate of correction was 90.00%.There was not significant change in SNB angle between before and after treatment,and the difference was not statistically significant(P>0.05).Compared with the indicators before treatment,OP-SN was decreased,while SN-MP,SNA and ANB were all increased after treatment,and the differences were statistically significant(t=6.402,6.135,3.753,50.965,P<0.05).There were not significant changes in Y-axis angle,Wits value,S-Go/N-Me and L1-NB angle between before and after treatment,and the differences were not statistically significant(P>0.05).Compared with the indicators before treatment,the NA-Apo and Go-Pg were increased after treatment,and the differences were statistically significant(t=46.885,7.575,P<0.05).Compared with the indicators before treatment,the NLA,H angle and UL-EP were increased,while LL-EP was decreased after treatment,and the differences were statistically significant(t=2.707,4.163,28.622,3.276,P<0.05).There was not significant change in L1-NB between before and after treatment,and the difference was not statistically significant(P>0.05).Compared with the indicators before treatment,the coverage,U1-SN and U1-NA were increased,while overbite and IMPA were decreased,and the differences were statistically significant(t=16.154,4.723,6.432,4.802,4.106,P<0.05).Conclusion:CBCT can clearly and accurately display three-dimensional morphology and changes of maxillofacial structure of oral cavity,which can provides comprehensive and reliable basis for the assessment for correction effect.Moreover,orthodontic treatment has positive significance in improving dentition occlusion relationship,guiding normal development of jawbone,and optimizing morphology of facial soft tissues,which can provide important reference for the formulation and optimization of the treatment scheme of clinical orthodontics.

Objective:To explore the relationship between microsatellite instability (MSI) and Ki-67 expression level and the clinicopathological features of colorectal cancer, and investigate their impact for prognosis, so as to provide reference for prognostic judgment of colorectal cancer.Methods:The data of 183 patients who underwent radical colorectal cancer surgery and were diagnosed pathologically in the Department of General Surgery, the First Affiliated Hospital of Xinxiang Medical University from January 2017 to December 2019 were retrospectively analysed, including 101 males (55.2%)and 82 females(44.8%), ranged from 20 to 86 years and the mean age was(60.27±13.13)years. According to the results of mismatch repair protein immunohistochemical staining, the patients were divided into MSI-H group ( n=32) and MSI-L/MSS group ( n=151). According to the results of Ki-67 antigen immunohistochemical staining, the patients were divided into low Ki-67 expression group (<82.5%, n=136) and high Ki-67 expression group (≥82.5%, n=47) , among which 62 cases (78.5%) with low Ki-67 expression and 17 cases (21.5%) with high Ki-67 expression were in patients with Ⅲ+ Ⅳ stage colorectal cancer. The data of clinicopathological features, disease-free survival, and overall survival were collected and analyzed. The cotegorical variables were presented as n(%), and the comparisons between groups were performed using Chi-square test or Fisher′s exact test. The multivariate Logistic regression model was used to estimate the correlation between microsatellite instability and Ki-67 expression level and clinicopathologic characteristics of colorectal cancer. Kaplan-Meier survival curve and COX proportional hazards regression model were used to analyze the correlation between microsatellite instability and Ki-67 expression level and disease-free survival and overall survival. Results:Single factor analysis showed that the differences in gender ( χ2=4.37, P=0.037), tumor site ( χ2=26.40, P<0.001), tumor maximum diameter ( χ2=11.12, P=0.001) and nerve invasion ( χ2=5.53, P=0.019) between MSI-H group and MSI-L/MSS group were statistically significant. Multivariate Logistic regression model analysis showed that only gender ( OR=3.013, 95% CI: 1.183-7.672, P=0.021), tumor location ( OR=0.167, 95% CI: 0.067-0.419, P<0.001) and nerve invasion ( OR=0.202, 95% CI: 0.042-0.968, P=0.045) were independently correlative factors for MSI status. In Ⅲ+ Ⅳ stage colorectal cancers, the difference in tumor site between low Ki-67 expression group and high Ki-67 expression group was statistically significant( χ2=3.91, P=0.048). Multivariate Cox proportional hazards regression model analysis revealed that high Ki-67 expression ( HR=0.301, 95% CI: 0.118-0.768, P=0.012; HR=0.275, 95% CI: 0.083-0.912, P=0.035) and MSI-H ( HR=0.072, 95% CI: 0.010-0.525, P=0.009; HR=0.122, 95% CI: 0.017-0.900, P=0.039) were independently protective factors for disease-free survival and overall survival. Conclusions:MSI-H colorectal cancer is common in males, right-sided colonic cancers and non-neuroinvasive patients. In stage Ⅲ+ Ⅳ colorectal cancer, the expression level of Ki-67 in right-sided colonic cancer was lower than in left-sided colorectal cancer. Patients with MSI-H and high Ki-67 expressive colorectal cancer had longer disease-free survival, longer overall survival and better prognosis.

Objective To investigate the expression of TROP2 and MMP-9 protein expression in cholangiocarcinomas and their relationship between the pathological behavior and prognosis.Methods A total of 54 patients who were diagnosed with cholangiocarcinoma in the People's Hospital of Binzhou,were retrospectively reviewed.Immunohistochemical staining and Log rank test were used to detect the expression of TROP2 and MMP-9 protein in 54 cases of cholangiocarcinomas and 18 cases of normal bile duct tissues achieved by partial hepatectomy of hepatolithiasis.Results The positive expression rate of TROP2 in cholangiocarcinoma tissues (55.6％) was higher than that of normal bile duct tissues (5.6％).The positive expression rate of MMP-9 in cholangiocarcinoma tissues (51.9％) was higher than that of normal bile duct tissues (11.1％).The differences of the expression of TROP2 and MMP-9 in cholangiocarcinoma of TNM stage,lymph node metastasis and neural invasion were significant(all P ＜ 0.05).There was significant positive correlation between TROP-2 and MMP-9 expression by using spearman correlation analysis (r =0.555,P ＜ 0.001).Survival analysis showed that TROP2 expression was an independent prognostic factor in cholangiocarcinoma.Conclusions TROP2 plays a important role in the development and metastasis of cholangiocarcinoma.Thus,TROP2 may be a prognostic indicator for cholangiocarcinoma.