Objective To investigate the expression of N-acetyltransferase 10(NAT10),serine hydroxymethyltransferase 2(SHMT2)and YTH domain family protein 1(YTHDF1)in lung cancer tissues and their correlation with clinicopathological characteristics and prognosis.Methods A total of 98 lung cancer patients admitted to our hospital from April 2020 to April 2021 were regarded as the observation subjects.The cancerous tissues and adjacent tissues of the patient during surgery were collected.Immunohistochemistry was used to detect the expression levels of NAT10,SHMT2 and YTHDF1.The patients were followed up for 3 years and divided into the survival group and the death group according to their prognosis.The data of general clinicopathological characteristics were collected and analyzed.The relationship between NAT10,SHMT2 and YTHDF1 with the prognosis of patients were analyzed.Multivariate Cox regression was used to analyze the influencing factors of lung cancer patients.Results The high expression ratios of NAT10,SHMT2 and YTHDF1 in cancer tissues were obviously higher than those in adjacent tissues(P＜0.05).The expression of NAT10 and YTHDF1 was related to clinical stage,degree of differentiation and lymph node metastasis(P＜0.05),and the expression of SHMT2 was correlated with clinical stage,degree of differentiation,tumor diameter,and lymph node metastasis(P＜0.05).There were statistically significant differences in tumor diameter,clinical stage,degree of differentiation,lymph node metastasis,and expressions of NAT10,SHMT2,and YTHDF1 between the survival group and the death group(P＜0.05).NAT10,SHMT2,YTHDF1 patient survival rates significantly below the low of high expression patients(x2=6.354,P=0.012,x2=8.512,P=0.004,x2=4.791,P=0.029).Lymph node metastasis,high expression of NAT10,SHMT2 and YTHDF1 are all risk factors affecting the prognosis of patients(P＜0.05).Conclusion NAT10,SHMT2,and YTHDF1 are all highly expressed in the tissues of lung cancer,and have a certain correlation with clinical pathological characteristics and prognosis.They may serve as relevant evaluation indicators for the prognosis of lung cancer patients.

Objective To investigate the expression of N-acetyltransferase 10(NAT10),serine hydroxymethyltransferase 2(SHMT2)and YTH domain family protein 1(YTHDF1)in lung cancer tissues and their correlation with clinicopathological characteristics and prognosis.Methods A total of 98 lung cancer patients admitted to our hospital from April 2020 to April 2021 were regarded as the observation subjects.The cancerous tissues and adjacent tissues of the patient during surgery were collected.Immunohistochemistry was used to detect the expression levels of NAT10,SHMT2 and YTHDF1.The patients were followed up for 3 years and divided into the survival group and the death group according to their prognosis.The data of general clinicopathological characteristics were collected and analyzed.The relationship between NAT10,SHMT2 and YTHDF1 with the prognosis of patients were analyzed.Multivariate Cox regression was used to analyze the influencing factors of lung cancer patients.Results The high expression ratios of NAT10,SHMT2 and YTHDF1 in cancer tissues were obviously higher than those in adjacent tissues(P＜0.05).The expression of NAT10 and YTHDF1 was related to clinical stage,degree of differentiation and lymph node metastasis(P＜0.05),and the expression of SHMT2 was correlated with clinical stage,degree of differentiation,tumor diameter,and lymph node metastasis(P＜0.05).There were statistically significant differences in tumor diameter,clinical stage,degree of differentiation,lymph node metastasis,and expressions of NAT10,SHMT2,and YTHDF1 between the survival group and the death group(P＜0.05).NAT10,SHMT2,YTHDF1 patient survival rates significantly below the low of high expression patients(x2=6.354,P=0.012,x2=8.512,P=0.004,x2=4.791,P=0.029).Lymph node metastasis,high expression of NAT10,SHMT2 and YTHDF1 are all risk factors affecting the prognosis of patients(P＜0.05).Conclusion NAT10,SHMT2,and YTHDF1 are all highly expressed in the tissues of lung cancer,and have a certain correlation with clinical pathological characteristics and prognosis.They may serve as relevant evaluation indicators for the prognosis of lung cancer patients.

Objective:To evaluate the value of contrast-enhanced ultrasound parameters, systemic immune-inflammation index (SII) and neutrophil to lymphocyte ratio (NLR) in the prognosis of prostate cancer after radical resection.Methods:The clinical data of 94 patients with radical resection of prostate cancer admitted to the Ankang Hospital of Traditional Chinese Medicine from February 2018 to February 2023 were retrospectively analyzed. The patients were followed up for 2 years after surgery. According to the follow-up results, the patients were divided into a recurrence group (37 cases) and a non-recurrence group (57 cases). Basic data [including age, body mass index (BMI), diabetes, hypertension, clinical stage, course of disease, Gleason score, surgical method, and American Society of Anesthesiologists (ASA) grade], preoperative SII and NLR, and postoperative 1 d contrast-enhanced ultrasound data of the two groups were collected, and compared between groups. The risk factors of postoperative recurrence in patients with prostate cancer were screened by unconditional logistic step-by-step regression analysis. The effectiveness of contrast ultrasound parameters, SII and NLR in evaluating postoperative recurrence of prostate cancer was analyzed by receiver operating characteristic (ROC) curve. At the same time, the prediction model of postoperative recurrence of prostate cancer patients was constructed and verified by ROC curve.Results:There were no significant differences in age, BMI, diabetes, hypertension, course of disease, surgical method and ASA classification between the two groups (all P>0.05). The proportion of clinical stage Ⅲ to Ⅳ, Gleason score, peak intensity (PI), mean time of passage (MTT), area under the curve (AUC), half time of intensity decline (HT), ascending branch slope (WIS), platelet count, SII and NLR in the non-recurrent group were lower than those in the recurrent group, and the differences were statistically significant (all P<0.05). Multivariate logistic regression analysis confirmed that clinical stage Ⅲ to Ⅳ, Gleason score, PI, MTT, AUC, HT, WIS, platelet count, SII and NLR were risk factors for postoperative recurrence of prostate cancer patients (all P<0.05). ROC curve analysis results showed that the areas under the curve of PI, MTT, AUC, HT, WIS, SII and NLR for postoperative recurrence of prostate cancer patients were 0.850, 0.923, 0.867, 0.856, 0.788, 0.746 and 0.932, respectively. The sensitivity was 0.811, 0.838, 0.784, 0.811, 0.703, 0.676, 0.892, respectively. According to the results of multivariate logistic regression analysis, the prediction model of postoperative recurrence of prostate cancer patients was fitted as Logit( P)=-3.661+ 0.464×clinical stage+ 0.611×Gleason score+ 0.617×PI+ 0.901×MTT+ 1.071×AUC+ 0.952×HT+ 0. 728×WIS+ 0.568×platelet count+ 0.628×SII+ 0.750×NLR. ROC curve results showed that the area under the curve of the model for predicting postoperative recurrence of prostate cancer patients was 0.953, standard error was 0.035, 95% CI was 0.884-1.000, sensitivity was 0.958, and specificity was 0.911. Conclusions:Contrast-enhanced ultrasound parameters, SII and NLR have certain value in the prognosis evaluation of prostate cancer after radical resection, and can be used as clinical indicators to evaluate whether the recurrence is possible.

Objective:To evaluate the value of contrast-enhanced ultrasound parameters, systemic immune-inflammation index (SII) and neutrophil to lymphocyte ratio (NLR) in the prognosis of prostate cancer after radical resection.Methods:The clinical data of 94 patients with radical resection of prostate cancer admitted to the Ankang Hospital of Traditional Chinese Medicine from February 2018 to February 2023 were retrospectively analyzed. The patients were followed up for 2 years after surgery. According to the follow-up results, the patients were divided into a recurrence group (37 cases) and a non-recurrence group (57 cases). Basic data [including age, body mass index (BMI), diabetes, hypertension, clinical stage, course of disease, Gleason score, surgical method, and American Society of Anesthesiologists (ASA) grade], preoperative SII and NLR, and postoperative 1 d contrast-enhanced ultrasound data of the two groups were collected, and compared between groups. The risk factors of postoperative recurrence in patients with prostate cancer were screened by unconditional logistic step-by-step regression analysis. The effectiveness of contrast ultrasound parameters, SII and NLR in evaluating postoperative recurrence of prostate cancer was analyzed by receiver operating characteristic (ROC) curve. At the same time, the prediction model of postoperative recurrence of prostate cancer patients was constructed and verified by ROC curve.Results:There were no significant differences in age, BMI, diabetes, hypertension, course of disease, surgical method and ASA classification between the two groups (all P>0.05). The proportion of clinical stage Ⅲ to Ⅳ, Gleason score, peak intensity (PI), mean time of passage (MTT), area under the curve (AUC), half time of intensity decline (HT), ascending branch slope (WIS), platelet count, SII and NLR in the non-recurrent group were lower than those in the recurrent group, and the differences were statistically significant (all P<0.05). Multivariate logistic regression analysis confirmed that clinical stage Ⅲ to Ⅳ, Gleason score, PI, MTT, AUC, HT, WIS, platelet count, SII and NLR were risk factors for postoperative recurrence of prostate cancer patients (all P<0.05). ROC curve analysis results showed that the areas under the curve of PI, MTT, AUC, HT, WIS, SII and NLR for postoperative recurrence of prostate cancer patients were 0.850, 0.923, 0.867, 0.856, 0.788, 0.746 and 0.932, respectively. The sensitivity was 0.811, 0.838, 0.784, 0.811, 0.703, 0.676, 0.892, respectively. According to the results of multivariate logistic regression analysis, the prediction model of postoperative recurrence of prostate cancer patients was fitted as Logit( P)=-3.661+ 0.464×clinical stage+ 0.611×Gleason score+ 0.617×PI+ 0.901×MTT+ 1.071×AUC+ 0.952×HT+ 0. 728×WIS+ 0.568×platelet count+ 0.628×SII+ 0.750×NLR. ROC curve results showed that the area under the curve of the model for predicting postoperative recurrence of prostate cancer patients was 0.953, standard error was 0.035, 95% CI was 0.884-1.000, sensitivity was 0.958, and specificity was 0.911. Conclusions:Contrast-enhanced ultrasound parameters, SII and NLR have certain value in the prognosis evaluation of prostate cancer after radical resection, and can be used as clinical indicators to evaluate whether the recurrence is possible.

OBJECTIVE：To investigate the situ ation and characteristics of ADR reports in the elderly patients （≥65 years old ） of Sichuan province ，and to provide reference for guaranteeing the safety of drug use. METHODS ：ADR reporting data of elderly patients collected by spontaneous reporting system of Sichuan ADR monitoring center from 2013 to 2019 were collected ，and then analyzed in respects of report types ，reproting institution ，relationship，patienst’age，gender，nationality，drug type ，organ/system invovled. RESULTS & CONCLUSIONS ：In the past seven years ，175 431 cases spontaneous ADR were collected by Sichuan ADR monitoring center ，and the annual proportion of “general”ADR reports decreased from 78.93％ in 2013 to 60.50％ year by year ； the annual proportion of “new general ”and“serious”ADRs increased year by year ，among which that of “new general ”ADR increased more obviously. The main reporting institutions were medical institutions ，accounting for 92.79％-98.87％ of the total annual reports. The enthusiasm of enterprises and individuals to report ADR needed to be improved ；8 031 cases（4.58％）were “positive”relationship，21 283 cases（12.13％）were“likely”，146 117 cases（83.29％）were“possible”. Except that the gender of 272 cases were not reported ，there were 88 176 male cases and 86 983 female cases ；most of them were 65-74 years old （104 962 cases，59.83％）. The reports covered 19 nationalities，among which the re were 166 752 cases（98.72％）of Han nationality. The distribution of ADR related drug categories in elderly patients of Yi ， Tibetan and Qiang nationalities was significantly different from that of Han nationality （P＜0.01）. The top three drug categories in the total frequency were anti-infective drugs （31.10％），traditional Chinese medicine com （18.27％），and central nervous system drugs （9.99％）. The injection route was the most likely to cause ADR （72.12％）. ADR mainly involved s kin and its appendants （21.47％）， gastrointestinal system （19.61％）and central and peripheral nervous system （18.55％）. Aminophylline injection was the only dr ug in the top 10 drugs leading to “new general ”ADR for consecutive 7 years. Cefotaxime sodium for injection ，Ceftriaxone sodium for injection ，Cefoperazone sodium and sulbactam sodium for injection were the top 10 drugs causing “severe”ADR in consecutive 7 years，which should be paid more attention in clinic.

OBJEVTIVETo investigate the expression of transient receptor potential lvanilloidreceptor 4 (TRPV4) protein in pemphigus vulgaris (PV), bullous pemphigoid (BP), dermatitis herpetiformis (DH), and epidermolysis bullosa acquisita (EBA), and explore the role of TRPV4 in the pathogenesis of these diseases.

METHODSTRPV4 protein in normal skin tissues and lesions of PV, BP, DH, and EBA were detected with immunohistochemistry.

RESULTSThe positivity rate of TRPV4 protein expression was 61.90% in PV, 81.81% in BP, 72.22% in DH, and 68.42% in EBA. TRPV4-positive rates in these lesions were significantly lower than the rate in normal skin tissues (93.33%) and also differed significantly among these lesions (PV

CONCLUSINLow TRPV4 expressions may affect the formation and reconstitution of skin connection. TRPV4 may play an role in the occurrence and development of autoimmune bullous skin disorders.

Dermatitis Herpetiformis ; metabolism ; Diagnosis, Differential ; Epidermolysis Bullosa Acquisita ; metabolism ; Humans ; Pemphigoid, Bullous ; metabolism ; Pemphigus ; metabolism ; Skin ; pathology ; TRPV Cation Channels ; metabolism

Objective To investigate the expression of transient receptor potential lvanilloidreceptor 4 (TRPV4) protein in pemphigus vulgaris (PV), bullous pemphigoid (BP), dermatitis herpetiformis (DH), and epidermolysis bullosa acquisita (EBA), and explore the role of TRPV4 in the pathogenesis of these diseases. Methods TRPV4 protein in normal skin tissues and lesions of PV, BP, DH, and EBA were detected with immunohistochemistry. Results The positivity rate of TRPV4 protein expression was 61.90% in PV, 81.81% in BP, 72.22% in DH, and 68.42% in EBA. TRPV4-positive rates in these lesions were significantly lower than the rate in normal skin tissues (93.33%) and also differed significantly among these lesions (PV

Objective To investigate the expression of transient receptor potential lvanilloidreceptor 4 (TRPV4) protein in pemphigus vulgaris (PV), bullous pemphigoid (BP), dermatitis herpetiformis (DH), and epidermolysis bullosa acquisita (EBA), and explore the role of TRPV4 in the pathogenesis of these diseases. Methods TRPV4 protein in normal skin tissues and lesions of PV, BP, DH, and EBA were detected with immunohistochemistry. Results The positivity rate of TRPV4 protein expression was 61.90% in PV, 81.81% in BP, 72.22% in DH, and 68.42% in EBA. TRPV4-positive rates in these lesions were significantly lower than the rate in normal skin tissues (93.33%) and also differed significantly among these lesions (PV

Objective To obtain the 3D shape data and features of 50th percentile posterior neck length in Chinese pilots.Methods The posterior neck length was measured in 150 fighter pilots by developed probe array and then 32 fighter pilots who were in accordance with 50th percentile posterior neck length were selected based on these 3D digitized posterior neck measurements.Results The method of digitized measurement was established.The range of posterior neck length was from 81 mm to 168 mm,the mean length was 127.3 mm in 150 pilots.In 32 fighter pilots with 50th percentile posterior neck length,the length range was from 120 mm to 123 mm,the mean length was 121.4 mm.The least square fitting curve was got then and 3D model was established.Conclusions The developed equipment and method are practical and effective.The results can be applied to the 3D analysis of posterior neck shape features and to the design of the neck support and head rest of ejection seat.

Objective To obtain the 3D shape data and features of 50th percentile posterior neck length in Chinese pilots.Methods The posterior neck length was measured in 150 fighter pilots by developed probe array and then 32 fighter pilots who were in accordance with 50th percentile posterior neck length were selected based on these 3D digitized posterior neck measurements.Results The method of digitized measurement was established.The range of posterior neck length was from 81 mm to 168 mm,the mean length was 127.3 mm in 150 pilots.In 32 fighter pilots with 50th percentile posterior neck length,the length range was from 120 mm to 123 mm,the mean length was 121.4 mm.The least square fitting curve was got then and 3D model was established.Conclusions The developed equipment and method are practical and effective.The results can be applied to the 3D analysis of posterior neck shape features and to the design of the neck support and head rest of ejection seat.