Hantavirus infection primarily causes hemorrhagic fever with renal syndrome (HFRS), leading to renal failure and systemic hemorrhage, while central nervous system (CNS) involvement is rare. In this case, a kidney transplant recipient was admitted due to memory loss and cognitive impairment without obvious precipitating factors. Metagenomic next-generation sequencing (mNGS) identified Hantavirus as the causative agent of CNS infection and its related complications. The patient achieved favorable outcomes through a combination of treatments, including ribavirin-based antiviral therapy, adjustment of immunosuppressive drug dosages, and immunomodulatory therapy with intravenous immunoglobulin (IVIG).

Recipients of organ transplants are prone to infection due to immunosuppressive agents. Compared to respiratory, urinary, and digestive system infection, central nervous system (CNS) infections exhibit lower incidence rates but carry poorer prognoses. Current epidemiological data remain limited, predominantly derived from case reports and single-center studies, with a notable absence of reliable diagnostic and therapeutic guidelines. This article comprehensively reviews domestic and international research and case reports on post-transplant CNS infection. From an etiological perspective, it elucidates the epidemiological and clinical characteristics of viral, bacterial, and fungal CNS infections, while summarizing diagnostic approaches and treatment strategies. The findings aim to provide clinical references for the management of central nervous system infection following organ transplantation.

Hantavirus infection primarily causes hemorrhagic fever with renal syndrome (HFRS), leading to renal failure and systemic hemorrhage, while central nervous system (CNS) involvement is rare. In this case, a kidney transplant recipient was admitted due to memory loss and cognitive impairment without obvious precipitating factors. Metagenomic next-generation sequencing (mNGS) identified Hantavirus as the causative agent of CNS infection and its related complications. The patient achieved favorable outcomes through a combination of treatments, including ribavirin-based antiviral therapy, adjustment of immunosuppressive drug dosages, and immunomodulatory therapy with intravenous immunoglobulin (IVIG).

Recipients of organ transplants are prone to infection due to immunosuppressive agents. Compared to respiratory, urinary, and digestive system infection, central nervous system (CNS) infections exhibit lower incidence rates but carry poorer prognoses. Current epidemiological data remain limited, predominantly derived from case reports and single-center studies, with a notable absence of reliable diagnostic and therapeutic guidelines. This article comprehensively reviews domestic and international research and case reports on post-transplant CNS infection. From an etiological perspective, it elucidates the epidemiological and clinical characteristics of viral, bacterial, and fungal CNS infections, while summarizing diagnostic approaches and treatment strategies. The findings aim to provide clinical references for the management of central nervous system infection following organ transplantation.

Objective:To summarize the experience of brain death determination under veno-arterial extracorporeal membrane oxygenation (VA-ECMO) assisted circulatory support, especially apnea test (AT) precautions, and to provide references for brain death determination in this scenario.Methods:In 78 patients who had VA-ECMO at Organ Transplant Center, Zhengzhou People's Hospital from October 2019 to December 2023, 8 organ donors had brain death determination under VA-ECMO assisted circulatory support. Baseline data, clinical data, and VA-ECMO data during AT trial were collected from these 8 patients to summarize the process of brain death determination.Results:Six of the 8 donors met the criteria of brain death; 10 EEG, 12 evoked potentials and 15 ATs were performed. Complications in ATs, including hypotension, decreased oxygenation and arrhythmia, were alleviated after timely improved VA-ECMO flow and applied cardiotonic and pressor drugs.Conclusion:AT is key for successful brain death determination in organ donors under VA-ECMO assisted circulatory support; therefore, complications should be closely monitored and managed.

Objective To evaluate the clinical outcome of kidney transplantation from donation after brain death(DBD)donors complicated with acute kidney injury(AKI).Methods Clinical data of 216 DBD donors were retrospectively analyzed,and they were divided into the AKI group(n=69)and control group(n=147)according to the Kidney Disease:Improving Global Outcomes(KDIGO)guidelines.Donors in the AKI group were further divided into the KDIGO stage 1 and stage 2-3 subgroups.One hundred and thirty-five recipients were assigned into the AKI group and 288 recipients in the control group.Postoperative recovery of renal function and clinical outcomes of the recipients were recorded.The risk factors of delayed graft function(DGF)were identified.Results The highest serum creatinine(Scr)level,Scr level before procurement,the highest blood sodium level and blood sodium level before procurement in the AKI group were higher than those in the control group.The application duration of vasopressors in the AKI group was longer than that in the control group.In the AKI group,the amount of fluid resuscitation within 48 h was higher,the HCO3-level at admission was lower,and the incidence of diabetes insipidus and hypotension was higher than those in the control group.The highest Scr level and the Scr level before procurement in KDIGO stage 2-3 donors were significantly higher than those in KDIGO stage 1 counterparts(all P＜0.05).Compared with the control group,the incidence of DGF and acute rejection was higher,the proportion of continuous renal replacement therapy was higher,the Scr level within postoperative 90 d was higher,and the urine amount within postoperative 3 d was less than those of recipients in the AKI group.Compared with KDIGO stage 1 recipients,KDIGO stage 2-3 recipients had higher Scr levels at postoperative 3,4,5 and 15 d,and less urine amount at postoperative 2 d(all P＜0.05).Univariate analysis showed that donor age,the highest Scr level,the highest blood sodium level and the amount of fluid resuscitation within 48 h were the risk factors for DGF in recipients after kidney transplantation.Multivariate analysis showed that donor age was the independent risk factor for DGF in recipients after kidney transplantation(all P＜0.05).Conclusions For the application of DBD donors complicated with AKI,active organ maintenance should be performed to alleviate AKI.It exerts no effect upon graft function and survival rate at postoperative 6 months,which may achieve equivalent efficacy as non-AKI donors and may be used as a source of extended criteria donor kidneys.

PURPOSE: The combination of indocyanine green and methylene blue (ICG + MB) was reported to be an efficient tracer method in sentinel lymph node biopsy (SLNB). However, whether this method is superior to MB only or carbon nanoparticles (CN) is controversial. This study was to evaluate the efficacy of the three methods in SLNB for breast cancer, and to analyze its influencing factors. METHODS: One hundred eighty patients with early breast cancer were recruited and randomly divided into 3 groups. Each group comprising of 60 patients with SLNB using ICG + MB, MB, and CN, respectively. Then the 3 groups were compared in detection rate, mean number of SLNs, and the detection rates and number of metastatic sentinel lymph nodes (SLNs). RESULTS: The detection rate of SLNs was 100% (60 of 60) in ICG + MB group, 96.7% (58 of 60), and 98.3% (59 of 60) in MB and CN group, respectively, with no significant difference (P = 0.362). Totally, 204 SLNs (mean ± standard deviation [SD] [range], 3.4 ± 1.4 [2–8]) were detected in ICG + MB group, 102 (1.7 ± 0.7 [0–3]) and 145 (2.4 ± 0.7 [0–6]) in MB and CN group, indicating significant difference (P < 0.001). The detection rate of metastatic SLN was 23.3% (14 of 60) in ICG + MB group, which was higher than 18.3% (11 of 60) and 20% (11 of 60) in MB and CN group, respectively, but showed no statistical significance (P = 0.788). CONCLUSION: ICG + MB method was superior to MB only and CN only methods in the mean number of SLNs, thus predicting axillary lymph node metastasis more accurately. Therefore, in areas where the standard method is not available, ICG + MB may be more suitable as an alternative tracer for SLNB.
Breast Neoplasms ; Breast ; Carbon ; Humans ; Indocyanine Green ; Lymph Nodes ; Methods ; Methylene Blue ; Nanoparticles ; Neoplasm Metastasis ; Sentinel Lymph Node Biopsy