Objective To explore the effects of miR-373 and Janus kinase 2/signal transducer and activator of transcription 6 (JAK2/STAT6) signaling pathways on the M2 polarization of tumor associated macrophages (TAM) in rectal cancer. Methods THP-1 cells were induced into M0/M1/M2 macrophages, M0 macrophages were cocultured with Caco-2 cells to obtain TAM, Flow cytometry was used to detect the expression of CD86 and CD206, Real-time quantitative qPCR and Western blot were used to detect miR-373, inducible nitric oxide synthase (iNOS), toll-like receptor 4 (TLR-4), interleukin 1β (IL-1β), tumor necrosis factor α (TNF-α), arginase 1 (Arg1), chitinase 3-like 1 (Ym1), resistin like α (Fizz1), IL-10 mRNA and protein levels. TAM were transfected and divided into overexpressing miR-373 group (miR-373-TAM) and control group (miR-NC-TAM), overexpressing miR-373+JAK2-TAM group (miR-373 combined with JAK2-TAM) and control group (miR-373 combined with NC-TAM), and then cocultured with Caco-2 cells. Flow cytometry was used to detect the expression of CD206 in TAM; Real-time quantitative PCR and Western blot were used to detect miR-373, Arg1, Ym1, Fizz1, IL-10, JAK2, STAT6 mRNA and protein levels in TAM; CCK-8 assay, colony formation assay, and Transwell assay were used to detect the proliferation, migration, and invasion ability of Caco-2 cells. Thirty nude mice were randomly divided into Caco-2 cells group, Caco-2 cells combined with miR-NC-TAM group, and Caco-2 cells combined with miR-373-TAM group, with 10 mice in each group. Rats in each group were subcutaneously injected with pure Caco-2 cells, Caco-2 cells combined with TAM, and Caco-2 cells combined with TAM overexpressing miR-373. After 4 weeks of cell inoculation, immunofluorescence staining was used to detect F4/80⁺CD206⁺cells level in tumor tissue; Real-time quantitative PCR and Western blot were used to detect miR-373, JAK2, STAT6, Arg1, Ym1, Fizz1, IL-10 mRNA and protein levels in tumor tissues. Results TAM tended to M2 polarization. After overexpression of miR-373, miR-373 level in TAM was increased, while Arg1, Ym1, Fizz1, IL-10, JAK2, STAT6 mRNA and protein levels were decreased, proliferation, migration, invasion ability of Caco-2 cells were decreased; Overexpression of JAK2 could partially reverse the effect of overexpression of miR-373 on the M2 polarization of TAM and proliferation, migration, invasion ability of Caco-2 cells. TAM could promote tumor growth; Overexpression of miR-373 could inhibit tumor growth and inhibit M2 polarization of TAM. Conclusion miR-373 could inhibit M2 polarization of TAM in rectal cancer, and miR-373 might inhibit proliferation and metastasis of rectal cancer cells by regulating the JAK2/STAT6 pathway.
MicroRNAs/metabolism* ; Humans ; STAT6 Transcription Factor/genetics* ; Signal Transduction/genetics* ; Animals ; Janus Kinase 2/genetics* ; Mice ; Tumor-Associated Macrophages/metabolism* ; Rectal Neoplasms/pathology* ; Caco-2 Cells ; Mice, Nude ; THP-1 Cells ; Mice, Inbred BALB C ; Cell Polarity ; Male

BACKGROUND: The mechanisms distinguishing metabolically healthy from unhealthy phenotypes within the same BMI categories remain unclear. This study aimed to investigate the associations between regional fat distribution and metabolically unhealthy phenotypes in Chinese adults across different BMI categories. METHODS: This cross-sectional study involving 11833 Chinese adults aged 20 years and older. Covariance analysis, adjusted for age, compared the percentage of regional fat (trunk, leg, or arm fat divided by whole-body fat) between metabolically healthy and unhealthy participants. Trends in regional fat percentage with the number of metabolic abnormalities were assessed by the Jonckheere-Terpstra test. Odds ratios (ORs) and their 95% confidence intervals (CIs) were estimated by logistic regression models. All analyses were performed separately by sex. RESULTS: In non-obese individuals, metabolically unhealthy participants exhibited higher percent trunk fat and lower percent leg fat compared to healthy participants. Additionally, percent trunk fat increased and percent leg fat decreased with the number of metabolic abnormalities. After adjustment for demographic and lifestyle factors, as well as BMI, higher percent trunk fat was associated with increased odds of being metabolically unhealthy [highest vs. lowest quartile: ORs (95%CI) of 1.64 (1.35, 2.00) for men and 2.00 (1.63, 2.46) for women]. Conversely, compared with the lowest quartile, the ORs (95%CI) of metabolically unhealthy phenotype in the highest quartile for percent arm and leg fat were 0.64 (0.53, 0.78) and 0.60 (0.49, 0.74) for men, and 0.72 (0.56, 0.93) and 0.46 (0.36, 0.59) for women, respectively. Significant interactions between BMI and percentage of trunk and leg fat were observed in both sexes, with stronger associations found in individuals with normal weight and overweight. CONCLUSIONS Trunk fat is associated with a higher risk of metabolically unhealthy phenotype, while leg and arm fat are protective factors. Regional fat distribution assessments are crucial for identifying metabolically unhealthy phenotypes, particularly in non-obese individuals.
Adult ; Aged ; Female ; Humans ; Male ; Middle Aged ; Young Adult ; Adipose Tissue ; Body Fat Distribution ; Body Mass Index ; China/epidemiology* ; Cross-Sectional Studies ; Health Surveys ; Phenotype

Objective:To investigate the interventional and protective effect of low-dose L-carnitine(LC)against reproductive system damage in male Wistar rats during acute exposure to simulated high-altitude environment.Methods:A total of 24 specific pathogen-free male Wistar rats,aged 12 weeks,were randomly divided into control group,high-altitude model group,and LC intervention group[intraperitoneal injection of LC at a dose of 50 mg/(kg·d)],with 8 rats in each group.The rats in the control group were fed under normal conditions(at an altitude of approximately 1 500 m),those in the high-altitude model group,and those in the LC intervention group were fed in a hypobaric oxygen chamber(at a simulated alti-tude of 6 000 m).The rats were sacrificed after 3 days.The testis was collected to calculate testicular index;the semen was collected from the epididymis,and the Weili sperm quality analysis system was used to assess sperm quality;blood samples were collected from the abdominal aorta,and ELISA kits were used to measure the serum levels of testosterone(T),luteinizing hormone(LH),and follicle-stimulating hormone(FSH);testicular tissue samples were collected,and biochemical kits were used to measure the activity of reactive oxygen species(ROS),malondialdehyde(MDA),and superoxide dismutase(SOD);testicular tissue was collected to prepare HE and electron microscopy sections,and a light microscope and a transmission electron microscope were used for observation.Results:Com-pared with the blank control group,the high-altitude model group had significant increases in the levels of T,LH,and FSH(P<0.01),testicular tissue damage under the light microscope,and changes in the morphology of spermatogenic cells,including mitochondrial al-terations,membrane edema,loss of cristae,swelling of the matrix,and local dissolution,as well as significant increases in the levels of ROS and MDA(P<0.01)and a significant reduction in SOD activity(P<0.01).Compared with the high-altitude model group,the LC intervention group had a significant increase in the level of T(P<0.01),significant reductions in the levels of FSH and LH(P<0.01),and significant improvements in the pathological changes of testicular tissue,with no marked mitochondrial injury,and there were sig-nificant reductions in the levels of ROS and MDA(P<0.01)and a significant increase in SOD activity(P<0.01).There was no signifi-cant difference in testicular index between groups(P>0.05).The high-altitude model group had a significantly lower sperm count than the blank control group(P<0.05),while there was no significant difference in sperm count between the LC intervention group and the blank control group(P>0.05);there was no significant difference in sperm motility between groups(P>0.05).Conclusion:Low-dose LC can improve reproductive system damage in rats during acute exposure to simulated high-altitude environment,possibly by alleviat-ing oxidative stress response.

Objective　To analyze the clinical features, etiological examination, laboratory examination, imaging examination, and treatment of patients with diabetes combined with Klebsiella pneumoniae liver abscess (KPLA), and to provide a reference for clinical work. Methods　The clinical data of patients with diabetes combined with KPLA in the First People's Hospital of Neijiang City from 2021 to 2022 were retrospectively analyzed. Results　Among the 12 patients with diabetes combined with KPLA, there were 8 males and 4 females, aged 47-74 years old. Five cases were combined with biliary tract diseases, 3 cases were combined with syphilis, and 2 cases were combined with hepatitis B.The main clinical manifestations included fever (12 cases), anorexia (10 cases), stomachache (6 cases), dizziness (2 cases), weakness (3 cases), cough and sputum (2 cases), disturbance of consciousness (2 cases), and visual impairment (1 case). Laboratory test results showed an increase in the percentage of neutrophils, glycosylated hemoglobin, transaminase, C-reactive protein, and procalcitonin, as well as a decrease in platelets and albumin. Abdominal CT findings were predominantly unilateral lesions, mostly found in the right liver, mainly manifested as mass, flaky, and nodular shadows. Two patients presented with invasive syndromes, 2 cases had lung abscesses, and 1 case had concomitant endophthalmitis and meningitis. The etiological examination of 12 patients with Klebsiella pneumoniae showed that they were all sensitive bacteria. After actively controlling blood glucose, effectively using antibiotics, and performing liver puncture and drainage, 11 cases improved, while 1 case was left with permanent blindness. Conclusions　The clinical features of diabetes combined with KPLA are atypical and easy to misdiagnose, thus early imaging and etiological examination should be performed. Aggressive glucose control, and selection of sensitive antimicrobial drugs based on drug sensitivity tests combined with liver abscess puncture and drainage can help to improve the prognosis.

Objective To quantitatively analyze Chinese Healthy China initiative policies and provide a basis for the formulation and improvement of relevant policies.Methods The Healthy China initiative policies released at the national and provincial levels from 2016 to 2023 was selected as the research object.A PMC index model containing 9 primary variables and 42 secondary variables was constructed using text mining methods to evaluate the quality of the Healthy China initiative policies.Results Using 32 selected Healthy China initiative policies as sample policies,the average PMC index is 7.81,and the policy quality is good or above,at a moderate depression level.The PMC index average values of policy nature,policy content,and policy evaluation are relatively high in the primary variables,and the shortcomings of policy incentives and effectiveness levels are more obvious.Conclusion The overall coverage of the Healthy China initiative policies is relatively comprehensive,but there are still problems such as weak effectiveness levels,insufficient use of diverse incentives and corresponding policy tools,and uneven regional development.In the future,improvements should be made in terms of enriching effectiveness levels,balancing the use of policy tools,and strengthening inter regional exchanges and learning.

Objective To quantitatively analyze Chinese Healthy China initiative policies and provide a basis for the formulation and improvement of relevant policies.Methods The Healthy China initiative policies released at the national and provincial levels from 2016 to 2023 was selected as the research object.A PMC index model containing 9 primary variables and 42 secondary variables was constructed using text mining methods to evaluate the quality of the Healthy China initiative policies.Results Using 32 selected Healthy China initiative policies as sample policies,the average PMC index is 7.81,and the policy quality is good or above,at a moderate depression level.The PMC index average values of policy nature,policy content,and policy evaluation are relatively high in the primary variables,and the shortcomings of policy incentives and effectiveness levels are more obvious.Conclusion The overall coverage of the Healthy China initiative policies is relatively comprehensive,but there are still problems such as weak effectiveness levels,insufficient use of diverse incentives and corresponding policy tools,and uneven regional development.In the future,improvements should be made in terms of enriching effectiveness levels,balancing the use of policy tools,and strengthening inter regional exchanges and learning.

Objective To quantitatively analyze Chinese Healthy China initiative policies and provide a basis for the formulation and improvement of relevant policies.Methods The Healthy China initiative policies released at the national and provincial levels from 2016 to 2023 was selected as the research object.A PMC index model containing 9 primary variables and 42 secondary variables was constructed using text mining methods to evaluate the quality of the Healthy China initiative policies.Results Using 32 selected Healthy China initiative policies as sample policies,the average PMC index is 7.81,and the policy quality is good or above,at a moderate depression level.The PMC index average values of policy nature,policy content,and policy evaluation are relatively high in the primary variables,and the shortcomings of policy incentives and effectiveness levels are more obvious.Conclusion The overall coverage of the Healthy China initiative policies is relatively comprehensive,but there are still problems such as weak effectiveness levels,insufficient use of diverse incentives and corresponding policy tools,and uneven regional development.In the future,improvements should be made in terms of enriching effectiveness levels,balancing the use of policy tools,and strengthening inter regional exchanges and learning.

Objective To quantitatively analyze Chinese Healthy China initiative policies and provide a basis for the formulation and improvement of relevant policies.Methods The Healthy China initiative policies released at the national and provincial levels from 2016 to 2023 was selected as the research object.A PMC index model containing 9 primary variables and 42 secondary variables was constructed using text mining methods to evaluate the quality of the Healthy China initiative policies.Results Using 32 selected Healthy China initiative policies as sample policies,the average PMC index is 7.81,and the policy quality is good or above,at a moderate depression level.The PMC index average values of policy nature,policy content,and policy evaluation are relatively high in the primary variables,and the shortcomings of policy incentives and effectiveness levels are more obvious.Conclusion The overall coverage of the Healthy China initiative policies is relatively comprehensive,but there are still problems such as weak effectiveness levels,insufficient use of diverse incentives and corresponding policy tools,and uneven regional development.In the future,improvements should be made in terms of enriching effectiveness levels,balancing the use of policy tools,and strengthening inter regional exchanges and learning.

Objective To quantitatively analyze Chinese Healthy China initiative policies and provide a basis for the formulation and improvement of relevant policies.Methods The Healthy China initiative policies released at the national and provincial levels from 2016 to 2023 was selected as the research object.A PMC index model containing 9 primary variables and 42 secondary variables was constructed using text mining methods to evaluate the quality of the Healthy China initiative policies.Results Using 32 selected Healthy China initiative policies as sample policies,the average PMC index is 7.81,and the policy quality is good or above,at a moderate depression level.The PMC index average values of policy nature,policy content,and policy evaluation are relatively high in the primary variables,and the shortcomings of policy incentives and effectiveness levels are more obvious.Conclusion The overall coverage of the Healthy China initiative policies is relatively comprehensive,but there are still problems such as weak effectiveness levels,insufficient use of diverse incentives and corresponding policy tools,and uneven regional development.In the future,improvements should be made in terms of enriching effectiveness levels,balancing the use of policy tools,and strengthening inter regional exchanges and learning.

Objective To quantitatively analyze Chinese Healthy China initiative policies and provide a basis for the formulation and improvement of relevant policies.Methods The Healthy China initiative policies released at the national and provincial levels from 2016 to 2023 was selected as the research object.A PMC index model containing 9 primary variables and 42 secondary variables was constructed using text mining methods to evaluate the quality of the Healthy China initiative policies.Results Using 32 selected Healthy China initiative policies as sample policies,the average PMC index is 7.81,and the policy quality is good or above,at a moderate depression level.The PMC index average values of policy nature,policy content,and policy evaluation are relatively high in the primary variables,and the shortcomings of policy incentives and effectiveness levels are more obvious.Conclusion The overall coverage of the Healthy China initiative policies is relatively comprehensive,but there are still problems such as weak effectiveness levels,insufficient use of diverse incentives and corresponding policy tools,and uneven regional development.In the future,improvements should be made in terms of enriching effectiveness levels,balancing the use of policy tools,and strengthening inter regional exchanges and learning.