Objective To investigate the effects of the rhein on the mitochondria fission and epithelial-mesenchymal transition(EMT)of breast cancer cells MDA-MB-231.Methods Human breast cancer cells were intervened with rhein,and the cells were divided into control group(0 μmol·L-1),low dose rhein group(100 μmol·L-1),and high dose rhein group(200 μmol·L-1).The proliferation activity of the cells was detected by CCK-8,and migrations was detected by Scratch-healing migration assay.The morphology and distribution of mitochondria were detected by transmission electron microscope,and the expression levels of Dynamin-related protein 1(Drp1),mitofusin2(Mfn2),E-cadherin,Vimentin proteins were detected by Western blot.Results Compared with control group,Rhein significantly reduced the protein expression of Drp1、Vimentin(P<0.05),and increased E-cadherin and Mfn2,thus down-regulating mitochondria fission,inhibiting cell proliferation and migration.High dose Rhein was better than low dose.Conclusion Rhein can inhibit the proliferation and migration of breast cancer cells by reducing the expression of Drp1,Vimentin and up-regulating Mfn2,E-cadherin proteins.

As one of the hallmarks of cancer, metabolic reprogramming leads to cancer progression, and targeting glycolytic enzymes could be useful strategies for cancer therapy. By screening a small molecule library consisting of 1320 FDA-approved drugs, we found that penfluridol, an antipsychotic drug used to treat schizophrenia, could inhibit glycolysis and induce apoptosis in esophageal squamous cell carcinoma (ESCC). Gene profiling and Ingenuity Pathway Analysis suggested the important role of AMPK in action mechanism of penfluridol. By using drug affinity responsive target stability (DARTS) technology and proteomics, we identified phosphofructokinase, liver type (PFKL), a key enzyme in glycolysis, as a direct target of penfluridol. Penfluridol could not exhibit its anticancer property in PFKL-deficient cancer cells, illustrating that PFKL is essential for the bioactivity of penfluridol. High PFKL expression is correlated with advanced stages and poor survival of ESCC patients, and silencing of PFKL significantly suppressed tumor growth. Mechanistically, direct binding of penfluridol and PFKL inhibits glucose consumption, lactate and ATP production, leads to nuclear translocation of FOXO3a and subsequent transcriptional activation of BIM in an AMPK-dependent manner. Taken together, PFKL is a potential prognostic biomarker and therapeutic target in ESCC, and penfluridol may be a new therapeutic option for management of this lethal disease.

At present, there are some problems in doctor-patient communication in standardized residency training in department of burns, such as insufficient understanding, lack of communication skills and short of teachers' guidance. Therefore, during the rotation of burns department, we should strengthen the cultivation of communication ability by improving humanistic care, professional level and communication training. At the same time, clinical teachers should strengthen their own quality and the assessment system should be improved. Through comprehensive efforts in many aspects, we will improve doctor-patient communication ability and build a harmonious doctor-patient relationship.

Metabolic disorder is one of the most obvious pathophysiological characteristics of patients with severe burn or trauma, which leads to high mortality of patients with severe burn or trauma. Metabonomics is a newly developed subject which provides new research concepts and ideas for studying the changes of metabolism in a disease condition. Based on the analysis of group indicators, metabonomic technique not only can systematically study the change rules of metabolites, which helps to further clarify the pathophysiological mechanism of burn or trauma, but also is helpful to find some significant biomarkers with important clinical value so as to provide new insight for the therapy of burn or trauma. This paper reviews the research progress of application of metabonomics in the treatment of severe burn or trauma in recent years.

Objective To investigate the incidence of motor system disorders or symptoms that occurred among naval afloat personnel onboard a certain naval surface ship after a prolonged distant voyage.Methods In the year of 2015,a randomized questionnaire survey was carried out among the naval afloat personnel,who experienced a prolonged ocean-going voyage greater than 5 months with a total distance as far as 32 500 nautical miles,covering 4 continents and crossing 2 great oceans.All the subjects finished the questionnaire survey independently and all the questionnaire survey forms were recovered on site and statistical analyses were made by Excel and SPSS 13.0 software.Results Totally,109 copies of questionnaire were issued and 95 copies were recovered,and 90 copies were confirmed valid,with a total valid rate of 94.7％.The average age of the subjects (11 females and 79 males) was (25.12 ± 4.63) years.There were 22 naval afloat personnel with past motor system chronic diseases,accounting for 24.4％.Before prolong distant voyage,52 people complained of motor system disorders (52/90),accounting for 58％,but after prolonged distant voyage,58 people complained of motor system disorders (58/90),accounting for 65.2％.Before prolonged distant voyage,in the ship crew who lived in the relatively dry cabins,22 people complained of motor system disorders (22/33),accounting for 66.7％,but after prolonged distant voyage,21 people complained of motor system disorders (21/33),accounting for 63.6％.However,Before prolonged distant voyage,in the ship crew who lived in the relatively humid cabins,25 people complained of motor system disorders (25/46),accounting for 54.3％,but after prolonged distant voyage,34 people complained of motor system disorders (34/46),accounting for 73.9％.Conclusions Various environmental factors during prolonged distant voyage,particularly humidity in living cabins,exerted negative effects on musculoskeletal system.More strict prophylactic countermeasures should be applied.Due to high incidence of musculoskeletal system disorders,poor knowledge of the naval ship crew about the prevention and treatment of the disorders and their urgent desire to obtain required necessary consultation on the disorders,corresponding medical service should be provided in accordance with the above-mentioned features.

Objective To investigate the incidence of motor system disorders or symptoms that occurred among naval afloat personnel onboard a certain naval surface ship after a prolonged distant voyage.Methods In the year of 2015,a randomized questionnaire survey was carried out among the naval afloat personnel,who experienced a prolonged ocean-going voyage greater than 5 months with a total distance as far as 32 500 nautical miles,covering 4 continents and crossing 2 great oceans.All the subjects finished the questionnaire survey independently and all the questionnaire survey forms were recovered on site and statistical analyses were made by Excel and SPSS 13.0 software.Results Totally,109 copies of questionnaire were issued and 95 copies were recovered,and 90 copies were confirmed valid,with a total valid rate of 94.7％.The average age of the subjects (11 females and 79 males) was (25.12 ± 4.63) years.There were 22 naval afloat personnel with past motor system chronic diseases,accounting for 24.4％.Before prolong distant voyage,52 people complained of motor system disorders (52/90),accounting for 58％,but after prolonged distant voyage,58 people complained of motor system disorders (58/90),accounting for 65.2％.Before prolonged distant voyage,in the ship crew who lived in the relatively dry cabins,22 people complained of motor system disorders (22/33),accounting for 66.7％,but after prolonged distant voyage,21 people complained of motor system disorders (21/33),accounting for 63.6％.However,Before prolonged distant voyage,in the ship crew who lived in the relatively humid cabins,25 people complained of motor system disorders (25/46),accounting for 54.3％,but after prolonged distant voyage,34 people complained of motor system disorders (34/46),accounting for 73.9％.Conclusions Various environmental factors during prolonged distant voyage,particularly humidity in living cabins,exerted negative effects on musculoskeletal system.More strict prophylactic countermeasures should be applied.Due to high incidence of musculoskeletal system disorders,poor knowledge of the naval ship crew about the prevention and treatment of the disorders and their urgent desire to obtain required necessary consultation on the disorders,corresponding medical service should be provided in accordance with the above-mentioned features.

Objective To discuss the benefits of extracorporeal membrane oxygenation (ECMO) applied in the patients with idiopathic pulmonary arterial hypertension during the operation of bilateral sequential single lung transplantation.Methods Fifteen patients with idiopathic pulmonary arterial hypertension received lung transplantation supported with ECMO between Nov.2009 and Sep.2014.ECMO was removed after the transplantation if the oxygenation and hemodynamic were stable,otherwise,ECMO was applied continuously until the situation improved.Results All the operations of 15 patients were accomplished successfully and the ECMO was removed in 3 patients after the operation immediately,while in the other 12 patients,ECMO was applied continuously until the situation improved in ICU.ECMO was applied again in 3 patients when it was necessary.Deep venous thrombosis occurred in one patient.Three patients died after operation and the other 12 patients are alive till now.Conclusion ECMO can be applied safely and effectively in lung transplantations for idiopathic pulmonary arterial hypertension patients.

Pulse contour cardiac output (PiCCO) monitoring is a new type of invasive hemodynamic monitoring technology, which is more and more often applied in perioperative period and the patients suffering from multiple injuries, septic shock, and extensive burn. With PiCCO one is able to monitor patients' hemodynamic indexes safely, timely, accurately, and continuously to provide reference for judgment of patients' condition and proper quality and quantity of fluid administration. This technique has a good prospect in clinical application.
Cardiac Output ; physiology ; Fluid Therapy ; methods ; Hemodynamics ; Humans ; Monitoring, Physiologic ; instrumentation

Objective To observe the effects of extracorporeal membrane oxygenation (ECMO) on coagulation during lung trangplantation.Method Forty cases of end stage lung diseases received bilateral sequential single lung transplantation during July 2007 and Mar.2012.The patients were divided into two groups in terms of ECMO.ECMO was applied before lung transplantation if needed.The amount of bleeding during surgery was recorded.The venous blood samples were collected during and after operation for the measurements of the following parameters:activated whole blood clot time (ACT),prothrombin time (PT),activated partial thromboplastin time (APTT),thrombin time (TT) and plasma fibrinogen (Fg).Result The coagulation and the amount of bleeding showed no significant difference between the two groups.Wound infection occurred in 2 patients where the ECMO tube was inserted and femoral arterial thrombosis in one patient.All of the three patients were cured and discharged.Conclusion ECMO didn't cause excessive bleeding or coagulation dysfunction during lung transplantation,yet it maybe increase the occurrence of local thrombosis.

Objective To evaluate the effects of ambroxol on prevention of bronchopulmonary complications after thoracotomy in patients with chronic obstructive pulmonary disease(COPD).MethodsTotal 161 elderly patients aged ≥70 years with COPD undergoing thoracotomy were randomly allocated to two groups:ambroxol group (300 mg/d,6 d) and placebo group as control.Pulmonary complications were evaluated by clinical symptoms,radiographic changes,and blood gas analysis. Results The incidence rates of atelectasis were 8.6％ in ambroxol group and 28.8％ in placebo group,respectively.The PaO2 values after surgery in ambroxol group decreased more than in placebo group (P＜0.05) compared with the preoperative values.Side effects were not found in all patients.Conclusions Ambroxol should be considered as an alternative pharmacologic approach for the prevention of post-thoracotomy pulmonary complications in the elderly patients with COPD.