Objective This study aimed to analyze the current status of depressive symptoms among middle school students in Jingzhou City in 2023 and the influence of daily lifestyle on these symptoms.Methods A convenience cluster sam-pling method was used to conduct a questionnaire survey among students in 25 middle schools in Jingzhou City from September 2023 to November 2023.The questionnaire included demographic information and standardized questionnaires.Chi-square test was used for univariate analysis,and binary logistic regression model was used for analysis of influencing factors.Results A-mong the 17,499 valid questionnaires,the prevalence of depressive symptoms among middle school students in Jingzhou City was 32.60％.Univariate analysis revealed that students with unhealthy lifestyles(including internet addiction,insomnia,lack of si-esta,frequent late-night activities,less participation in physical exercise,passive participation in group activities,and unbal-anced dietary habits)had a higher prevalence of depressive symptoms.Moreover,high school students(40.95％)had a higher prevalence than middle school students(27.57％),and females(37.43％)had a higher prevalence than males(27.96％);all differences were statistically significant(P＜0.001).Binary logistic regression analysis showed that being female,being a high school student,internet addiction,insomnia,lack of siesta,frequent late-night activities,less participation in physical exercise,passive participation in group activities,and unbalanced dietary habits were risk factors for depressive symptoms among middle school students in Jingzhou City.Conclusion This study revealed the association between depressive symptoms and daily lifes-tyle among middle school students in Jingzhou City.The prevalence of depressive symptoms was related to factors including being female,being a high school student,internet addiction,insomnia,lack of siesta,unbalanced dietary habits,less participation in physical exercise,and passive participation in group activities.It is recommended to focus on cultivating good daily behavioral habits among students in both school and family environments to improve their mental health.

Objective This study aimed to analyze the current status of depressive symptoms among middle school students in Jingzhou City in 2023 and the influence of daily lifestyle on these symptoms.Methods A convenience cluster sam-pling method was used to conduct a questionnaire survey among students in 25 middle schools in Jingzhou City from September 2023 to November 2023.The questionnaire included demographic information and standardized questionnaires.Chi-square test was used for univariate analysis,and binary logistic regression model was used for analysis of influencing factors.Results A-mong the 17,499 valid questionnaires,the prevalence of depressive symptoms among middle school students in Jingzhou City was 32.60％.Univariate analysis revealed that students with unhealthy lifestyles(including internet addiction,insomnia,lack of si-esta,frequent late-night activities,less participation in physical exercise,passive participation in group activities,and unbal-anced dietary habits)had a higher prevalence of depressive symptoms.Moreover,high school students(40.95％)had a higher prevalence than middle school students(27.57％),and females(37.43％)had a higher prevalence than males(27.96％);all differences were statistically significant(P＜0.001).Binary logistic regression analysis showed that being female,being a high school student,internet addiction,insomnia,lack of siesta,frequent late-night activities,less participation in physical exercise,passive participation in group activities,and unbalanced dietary habits were risk factors for depressive symptoms among middle school students in Jingzhou City.Conclusion This study revealed the association between depressive symptoms and daily lifes-tyle among middle school students in Jingzhou City.The prevalence of depressive symptoms was related to factors including being female,being a high school student,internet addiction,insomnia,lack of siesta,unbalanced dietary habits,less participation in physical exercise,and passive participation in group activities.It is recommended to focus on cultivating good daily behavioral habits among students in both school and family environments to improve their mental health.

Objective To investigate the activation of local renin-angiotensin system induced by TLR4 signaling pathway in the adipose tissues in diet-induced obesity(DIO)mice. Methods C57BL/6 mice,six-week-old,male,were divided randomly into normal-diet group and high-fat diet group. High-fat diet group was fed for 14 weeks. Mice,which weighted up 20%,were divided into 3 groups:obesity-group(OB,n=5),fenofibrate group (FF,n = 5)and TAK242 group(TAK,n = 5). The fenofibrate group was given fenofibrate(100 mg/kg/day)by oral gavage for a further 2 weeks and the TAK242 group TAK-242(3 mg/kg/d)by intraperitoneal injection for 2 weeks. The 23-week-aged mice were sacrificed and the triglyceride(TG),nonesterified fatty acid(NEFA),glutamic-pyruvic transaminase(ALT),aspartate aminotransferase(AST)were measured. NEFA in liver tissues were measured with ELISA and the expression levels of AT1R,AGT and TLR4 were measured by RT-PCR. Results TAK-242(Resatorvid)inhibited the expressions of AT1R,AGT and TLR4 in adipose tissues. The effects of fenofi-brate were different in adipose tissues and liver tissues. Conclusion Activation of TLR4 signaling path way ac-tives the local RAS in adipose tissues. The expressions of AT1R and AGT in adipose tissue are significantly inhibit-ed by TAK-242.