Objective:To investigate the risk factors associated with the development of venous thromboembolism with abdominal infection following pancreatic cancer surgery.Methods:A retrospective analysis was conducted on the clinical data of 70 patients with pancreatic cancer who underwent pancreaticoduodenectomy at the Department of Hepatobiliary Surgery of the First Affiliated Hospital of Air Force Medical University from March 2020 to August 2024, of which 31 were male and 39 were female, aged (58.5±6.8) years. Two groups were divided into two groups according to the occurrence of venous thromboembolism with abdominal infection in the postoperative period: the complication group ( n=28) and the non-complication group ( n=42). The general data, laboratory indexes and surgery-related indexes of the two groups were recorded, and logistic regression model was used to analyse the risk factors for the occurrence of venous thromboembolism with abdominal cavity infection after pancreaticoduodenectomy, and receiver operating characteristic (ROC) curve was used to assess the predictive value of the factors for the occurrence of venous thromboembolism with abdominal cavity infection after pancreatic cancer surgery. Results:Multifactorial analysis showed that the combination of diabetes mellitus ( OR=1.216, 95% CI: 1.225-1.657, P=0.035), occurrence of postoperative pancreatic fistula ( OR=1.292, 95% CI: 1.095-1.829, P=0.022), occurrence of postoperative biliary fistula ( OR=1.239, 95% CI: 1.171-2.618, P=0.005), high D-dimer ( OR=137.030, 95% CI: 3.214-5 843.128, P=0.010), prolonged operation time ( OR=1.058, 95% CI: 1.002-1.117, P=0.042), and low serum albumin ( OR=0.711, 95% CI: 0.508-0.995, P=0.046) in pancreatic cancer patients with a high risk of postoperative venous thromboembolism with the development of abdominal infection. ROC curve analysis showed that diabetes mellitus, pancreatic fistula, biliary fistula, D-dimer, duration of surgery, serum albumin, and co-prediction had certain predictive value for the occurrence of postoperative venous thromboembolism with abdominal infection after pancreatic cancer surgery, in which, the area under the curve for co-prediction in assessing the occurrence of postoperative venous thromboembolism with abdominal infection after pancreatic cancer surgery was 0.993 (95% CI: 0.982-1.000), with a sensitivity of 1.000 and a specificity of 0.929. Conclusion:Comorbid diabetes mellitus, pancreatic fistula, biliary fistula, D-dimer, prolonged duration of surgery, and serum albumin are independent risk factors for the development of postoperative venous thromboembolism with abdominal infection in patients with pancreatic cancer, all of which have diagnostic value and a high joint predictive value. The predictive value of co-prediction is relatively high.

Objective To explore the clinical effect of quality control circle(QCC)activities on the prevention of postoperative deep vein thrombosis(DVT)in inpatients in military hospitals.Methods A total of 318 patients who were diagnosed and treated in The First Affiliated Hospital of Air Force Medical University from January to December 2021 and 40 medical staff were enrolled in this study.Routine care was performed in 158 patients from January to June 2021,and QCC care was implemented in 160 patients from July to December 2021.The awareness of DVT prevention in medical staff and patients(or their famiy members)before and after QCC activities,lower limb DVT preventive measures taken by medical staff,and the occurrence of DVT were compared.Results The scores of the cause,clinical manifestations,nursing measures and preventive measures of DVT after QCC activities were significantly higher than those before QCC activities in both medical staff and patients(or their families)(P<0.05).The overall implementation rate of preventing lower limb DVT after QCC activities was significantly higher than that before QCC activities(94.14%vs.46.20%,P<0.05).The incidence of DVT after QCC activities was significantly lower than that before QCC activities(0.62%vs.5.06%,P<0.05).Conclusion Implementing QCC activities can improve the cognitive ability of military medical staff and patients(or family members)in DVT prevention,increase the implementation rate of DVT prevention measures,and reduce the incidence of DVT.

Objective To investigate the effect of Danshou decoction on autophagy,proliferation and apoptosis of human villous trophoblast cell HTR-8/Svneo by regulating the mTOR/S6K pathway.Methods HTR-8/Svneo cells were divided into control,Danshou decoction low-dose(Danshou decoction-L,100 μmol/mL),medium-dose(Danshou decoction-M,150 μmol/mL),high-dose(Danshou decoction-H,200 μmol/mL),the mTOR pathway activator MHY1485(100 nmol/mL),and Danshou decoction-H+M HY1485 groups.After 24 hours of treatment,cell proliferation,apoptosis,invasion,and migration were detected by using CCK-8,flow cytometry,Transwell,and wound healing assays.Autophagosome changes were observed,and the expres-sion levels of LC3,p62,Beclin1 mRNA and mTOR/S6K pathway related proteins were detected.Results Compared with the control group,the proliferation rate,invasion rate,migration rate,the proportions of autophagosomes/total cytoplasmic area,LC3,Beclin1 mRNA,p-mTOR/mTOR,and S6K protein expression reduced,and the apoptosis rate and p62 mRNA expression increased in Danshou decoction group at different dosages.The proliferation rate,invasion rate,migration rate,the proportion of autophagosomes/total cytoplasmic area,LC3,Beclin1 mRNA,p-mTOR/mTOR,and S6K protein expression increased,and the apoptosis rate and p62 mRNA expression decreased in the MHY1485 group(all P＜0.05).Compared with the Danshou decoc-tion-H group,the proliferation rate,invasion rate,migration rate,the proportion of autophagosomes/total cytoplasmic area,LC3,Beclin1 mRNA,p-mTOR/mTOR,and S6K protein expression increased,and the apoptosis rate and p62 mRNA expression decreased in the Danshou decoction-H+M HY1485 group(all P＜0.05).Conclusion Danshou decoction inhibits autophagy and proliferation,and induces apoptosis of human villous trophoblast cell HTR-8/Svneo by regulating the mTOR/S6K pathway.

Objective To investigate the effect of Danshou decoction on autophagy,proliferation and apoptosis of human villous trophoblast cell HTR-8/Svneo by regulating the mTOR/S6K pathway.Methods HTR-8/Svneo cells were divided into control,Danshou decoction low-dose(Danshou decoction-L,100 μmol/mL),medium-dose(Danshou decoction-M,150 μmol/mL),high-dose(Danshou decoction-H,200 μmol/mL),the mTOR pathway activator MHY1485(100 nmol/mL),and Danshou decoction-H+M HY1485 groups.After 24 hours of treatment,cell proliferation,apoptosis,invasion,and migration were detected by using CCK-8,flow cytometry,Transwell,and wound healing assays.Autophagosome changes were observed,and the expres-sion levels of LC3,p62,Beclin1 mRNA and mTOR/S6K pathway related proteins were detected.Results Compared with the control group,the proliferation rate,invasion rate,migration rate,the proportions of autophagosomes/total cytoplasmic area,LC3,Beclin1 mRNA,p-mTOR/mTOR,and S6K protein expression reduced,and the apoptosis rate and p62 mRNA expression increased in Danshou decoction group at different dosages.The proliferation rate,invasion rate,migration rate,the proportion of autophagosomes/total cytoplasmic area,LC3,Beclin1 mRNA,p-mTOR/mTOR,and S6K protein expression increased,and the apoptosis rate and p62 mRNA expression decreased in the MHY1485 group(all P＜0.05).Compared with the Danshou decoc-tion-H group,the proliferation rate,invasion rate,migration rate,the proportion of autophagosomes/total cytoplasmic area,LC3,Beclin1 mRNA,p-mTOR/mTOR,and S6K protein expression increased,and the apoptosis rate and p62 mRNA expression decreased in the Danshou decoction-H+M HY1485 group(all P＜0.05).Conclusion Danshou decoction inhibits autophagy and proliferation,and induces apoptosis of human villous trophoblast cell HTR-8/Svneo by regulating the mTOR/S6K pathway.

Objective:To investigate the risk factors associated with the development of venous thromboembolism with abdominal infection following pancreatic cancer surgery.Methods:A retrospective analysis was conducted on the clinical data of 70 patients with pancreatic cancer who underwent pancreaticoduodenectomy at the Department of Hepatobiliary Surgery of the First Affiliated Hospital of Air Force Medical University from March 2020 to August 2024, of which 31 were male and 39 were female, aged (58.5±6.8) years. Two groups were divided into two groups according to the occurrence of venous thromboembolism with abdominal infection in the postoperative period: the complication group ( n=28) and the non-complication group ( n=42). The general data, laboratory indexes and surgery-related indexes of the two groups were recorded, and logistic regression model was used to analyse the risk factors for the occurrence of venous thromboembolism with abdominal cavity infection after pancreaticoduodenectomy, and receiver operating characteristic (ROC) curve was used to assess the predictive value of the factors for the occurrence of venous thromboembolism with abdominal cavity infection after pancreatic cancer surgery. Results:Multifactorial analysis showed that the combination of diabetes mellitus ( OR=1.216, 95% CI: 1.225-1.657, P=0.035), occurrence of postoperative pancreatic fistula ( OR=1.292, 95% CI: 1.095-1.829, P=0.022), occurrence of postoperative biliary fistula ( OR=1.239, 95% CI: 1.171-2.618, P=0.005), high D-dimer ( OR=137.030, 95% CI: 3.214-5 843.128, P=0.010), prolonged operation time ( OR=1.058, 95% CI: 1.002-1.117, P=0.042), and low serum albumin ( OR=0.711, 95% CI: 0.508-0.995, P=0.046) in pancreatic cancer patients with a high risk of postoperative venous thromboembolism with the development of abdominal infection. ROC curve analysis showed that diabetes mellitus, pancreatic fistula, biliary fistula, D-dimer, duration of surgery, serum albumin, and co-prediction had certain predictive value for the occurrence of postoperative venous thromboembolism with abdominal infection after pancreatic cancer surgery, in which, the area under the curve for co-prediction in assessing the occurrence of postoperative venous thromboembolism with abdominal infection after pancreatic cancer surgery was 0.993 (95% CI: 0.982-1.000), with a sensitivity of 1.000 and a specificity of 0.929. Conclusion:Comorbid diabetes mellitus, pancreatic fistula, biliary fistula, D-dimer, prolonged duration of surgery, and serum albumin are independent risk factors for the development of postoperative venous thromboembolism with abdominal infection in patients with pancreatic cancer, all of which have diagnostic value and a high joint predictive value. The predictive value of co-prediction is relatively high.

Objective To analyze the qualified rate of the fiducial markers during 3D-printing co-planar template assisted CT-guided implantation in stereotactic body radiation therapy by CyberKnife,and to explore the cause of the unavailable markers in order to provide the reference for the fiducial implantation,treatment planning and radiotherapy delivery.Methods From March to December 2017,a total of 52 cases were planned to stereotactic body radiation therapy(SBRT) using CyberKnife by fiducial tracking,and the fiducial markers were implanted based on CT-guided 3D-printing co-planar template,including 22 in lung,12 in liver,5 in mediastinal lymph node,8 in retroperitoneal lymph node,3 in pancreas,each in celiac and pelvic lymph nodes,respectively.Except 7 cases not fit for CyberKnife treatment,45 cases finished the treatment of CyberKnife,but there were 3 cases changed to spine tracking due to unqualified fiducial markers.The number of fiducial markers used and the qualified rate of fiducial markers were analyzed,and the cause of unqualified fiducial markers was studied.Results A total of 131 fiducial markers were impanted into 42 cases who finally received the treatment of CyberKnife by fiducial tracking,including 85 fiducial markers qualified (64.89％) and 46 fiducial markers unqualified (35.11％).The main causes of the unqualified fiducial markers varied,including outrange of rigidity error(26.08％),fiducial markers unavailable(41.31％),and other (32.61％).Conclusions The 3D-printing co-planar template assisted CT-guided implantation could reduce the number of puncture needles used,help to decrease the risk of puncture and trauma and the incidence of complications after the fiducial markers implantation.However,the fiducial markers implanted by this way would be abandoned by a variety of causes and should be taken into account before the fiducial markers implantation.