A 78-year-old man received intravenous infusion of ceftizoxime sodium 2. 25 g once daily,oral carbamazepine 0. 2 g twice daily,oral nimesulide 100 mg twice daily,intravenous infusion of dihydroxypropyl theophylline 0. 5 g once daily,oral mecobalamin 0. 5 mg thrice daily,intravenous infusion of dexamethasone 5 mg for one time,intramuscular injection of meperidine hydrochloride 25 mg for one time, and intramuscular injection of bucinnazine hydrochloride 100 mg for three times for postherpetic neuralgia and chronic obstructive pulmonary diseases with infection. On day 7,ceftizoxime sodium was stopped and changed to intravenous infusion of fosfomycin sodium 8 g once daily. On day 11, his blood routine examination showed the following levels:white blood cell count 1. 6 × 109/L with neutrophils 0. 03,neutrophil absolute count 0. 1 × 109/L,and lymphocyte absolute count 0. 9 × 109/L. All the drugs were discontinued and symptomatic and supportive treatment was given. On day 15,the level of white blood cell became 0. 9 × 109/L with neutrophils 0. 02,a absolute value of neutrophil and lymphocyte were respectively 0. 1 × 109/L and 0. 7 × 109/L. According to bone marrow smear histology,agranulocytosis was diagnosed. On day 17, the patient developed pneumothorax and atelectasis in the right lung. On day 20,acute respiratory failure and multiple organ failure occurred with infection and the resuscitation was unsuccessful.

A 78-year-old man received intravenous infusion of ceftizoxime sodium 2. 25 g once daily,oral carbamazepine 0. 2 g twice daily,oral nimesulide 100 mg twice daily,intravenous infusion of dihydroxypropyl theophylline 0. 5 g once daily,oral mecobalamin 0. 5 mg thrice daily,intravenous infusion of dexamethasone 5 mg for one time,intramuscular injection of meperidine hydrochloride 25 mg for one time, and intramuscular injection of bucinnazine hydrochloride 100 mg for three times for postherpetic neuralgia and chronic obstructive pulmonary diseases with infection. On day 7,ceftizoxime sodium was stopped and changed to intravenous infusion of fosfomycin sodium 8 g once daily. On day 11, his blood routine examination showed the following levels:white blood cell count 1. 6 × 109/L with neutrophils 0. 03,neutrophil absolute count 0. 1 × 109/L,and lymphocyte absolute count 0. 9 × 109/L. All the drugs were discontinued and symptomatic and supportive treatment was given. On day 15,the level of white blood cell became 0. 9 × 109/L with neutrophils 0. 02,a absolute value of neutrophil and lymphocyte were respectively 0. 1 × 109/L and 0. 7 × 109/L. According to bone marrow smear histology,agranulocytosis was diagnosed. On day 17, the patient developed pneumothorax and atelectasis in the right lung. On day 20,acute respiratory failure and multiple organ failure occurred with infection and the resuscitation was unsuccessful.

OBJECTIVETo explore the feasibility of genetic algorithm (GA) on multiple objective blending technology for extractions of Cortex Fraxini.

METHODAccording to that the optimization objective was the combination of fingerprint similarity and the root-mean-square error of multiple key constituents, a new multiple objective optimization model of 10 batches extractions of Cortex Fraxini was built. The blending coefficient was obtained by genetic algorithm. The quality of 10 batches extractions of Cortex Fraxini that after blending was evaluated with the finger print similarity and root-mean-square error as indexes.

RESULTThe quality of 10 batches extractions of Cortex Fraxini that after blending was well improved. Comparing with the fingerprint of the control sample, the similarity was up, but the degree of variation is down. The relative deviation of the key constituents was less than 10%.

CONCLUSIONIt is proved that genetic algorithm works well on multiple objective blending technology for extractions of Cortex Fraxini. This method can be a reference to control the quality of extractions of Cortex Fraxini. Genetic algorithm in blending technology for extractions of Chinese medicines is advisable.

Algorithms ; Chromatography, High Pressure Liquid ; Drugs, Chinese Herbal ; analysis ; Medicine, Chinese Traditional ; standards ; Plants, Medicinal ; chemistry ; genetics ; Quality Control