To explore the interaction between ASFV capsid protein M1249L and host from the host cellular perspective,M1249L was selected for constructing the bait plasmid(pGBKT7-M1249L)to screen the bone marrow-derived macrophages(BMDMs)cDNA library.After again co-transform and sequence alignment,20 candidate interacting host proteins were screened,such as IL-1β,CTSB and DNAJA3.And then,co-immunoprecipitation assay was performed to verify the interaction be-tween M1249L and host proteins.GO ontology(GO)and KEGG pathway enrichment analyses re-vealed that biological regulation,cellular communication and response to stimulus and others were enriched in biological processes.And these host proteins could share some pathways,including toll-like receptor signaling pathway and Nod-like receptor signaling pathway.Therefore,the results provides the theoretical basis for further research on the mechanism of ASFV M1249L in viral in-fection and immune regulation.

To explore the interaction between ASFV capsid protein M1249L and host from the host cellular perspective,M1249L was selected for constructing the bait plasmid(pGBKT7-M1249L)to screen the bone marrow-derived macrophages(BMDMs)cDNA library.After again co-transform and sequence alignment,20 candidate interacting host proteins were screened,such as IL-1β,CTSB and DNAJA3.And then,co-immunoprecipitation assay was performed to verify the interaction be-tween M1249L and host proteins.GO ontology(GO)and KEGG pathway enrichment analyses re-vealed that biological regulation,cellular communication and response to stimulus and others were enriched in biological processes.And these host proteins could share some pathways,including toll-like receptor signaling pathway and Nod-like receptor signaling pathway.Therefore,the results provides the theoretical basis for further research on the mechanism of ASFV M1249L in viral in-fection and immune regulation.

Objective To design a mobile personnel radiation protection equipment for operation in environments with high radiation such as spent fuel reprocessing plants, to achieve simultaneous protection against γ radiation, neutron radiation, and radioactive aerosol, to reduce the internal and external exposure dose of radioactive workers, and to meet the requirement of operation for two hours. Methods The core parts of the mobile personnel radiation protection equipment included a shielding chamber and a respiratory maintenance system. An automated chassis was used for the movement and lifting of the shielding chamber. MCNP software was used to simulate and calculate the protective effects of shielding chamber made of different materials and material thicknesses. Experimental verification of the shielding chamber design was conducted. Mathematical models were established to describe the variations in the content of various gases in the chamber with personnel operation time. A respiratory maintenance system, a harmful gas absorption device, and an automated mobile chassis were designed. Results The shielding chamber made of polyethylene with a thickness of 80 mm achieved an 80% neutron shielding rate. The respiratory maintenance system could support workers for 2 hours of operation inside the equipment. The mobile chassis allowed operation of the equipment with one person. Conclusion This mobile personnel radiation protection equipment can solve the problem in simultaneous protection against γ radiation, neutron radiation, and radioactive aerosol. The equipment can provide radiation protection for radioactive workers, reduce exposure dose, and reduce personnel burden. This system provides technical means for the operation and maintenance of equipment in high-radiation sites such as spent fuel reprocessing plants.

Improving the reproducibility of biomedical research results is a major challenge. Transparent and accurate reporting of the research process enables readers to evaluate the reliability of the research results and further explore the experiment by repeating it or building upon its findings. The ARRIVE 2.0 guidelines, released in 2019 by the UK National Centre for the Replacement, Refinement and Reduction of Animals in Research (NC3Rs), provide a checklist that is applicable to any in vivo animal research report. These guidelines aim to improve the standardization of experimental design, implementation, and reporting, as well as enhance the reliability, repeatability, and clinical translation of animal experimental results. The use of the ARRIVE 2.0 guidelines not only enriches the details of animal experimental research reports, ensuring that information on animal experimental results is fully evaluated and utilized, but also enables readers to understand the content expressed by the author accurately and clearly, promoting the transparency and completeness of the fundamental research review process. At present, the ARRIVE 2.0 guidelines have been widely adopted by international biomedical journals. This article is based on the best practices following the ARRIVE 2.0 guidelines in international journals, and it interprets, explains, and elaborates in Chinese the fifth part of the comprehensive version of the ARRIVE 2.0 guidelines published in PLoS Biology in 2020 (the original text can be found at https://arriveguidelines.org). This section includes the items 6-11 of Recommended 11 section, covering "Animal Care and Monitoring", "Interpretation/Scientific Implications", "Generalisability/Translation", "Protocol Registration", "Data Access" and "Declaration of Interests". Its aim is to promote a comprehensive understanding and use of the ARRIVE 2.0 guidelines among domestic researchers, to enhance the standardization of experimental animal research and reporting, and to promote high-quality development of experimental animal sciences and comparative medicine research in China.

Objective To compare and analyze the difference of cardiopulmonary reserve function of phlegm-dampness and peaceful constitutions. Methods A total of 102 cases were selected for the physi-cal examination in Air Service Department of Northern Theater Air Force Hospital,and divided into a phlegm-dampness constitution group of 50 and a peaceful constitution group of 52 according to their traditional Chinese medicine constitution. Both groups underwent the cardiopulmonary exercise testing (CPET),and the cardiopulmonary reserve function parameters such as the peak exercise load,peak metabolic equivalent,peak kilogram oxygen uptake,and peak oxygen pulse rate were compared be-tween the 2 groups. Results There was no significant difference between the 2 groups in age,as well as the history of smoking and drinking(P>0.05). Moreover,the average body mass index,neck circum-ference,heart rate reserve,and peak ventilation of the phlegm-dampness constitution group were signif-icantly higher than the peaceful constitution group,while the average peak exercise load,peak meta-bolic equivalent,peak heart rate,peak oxygen pulse,and peak kilogram oxygen uptake were signifi-cantly lower than the latter group(P<0.05). Conclusions Compared with the people of peaceful constitu-tion,those of phlegm-dampness have lower cardiopulmonary reserve function. Therefore,early physical intervention should be conducted to help them to get into a healthy lifestyle.

Objective:To explore the effect and correlation of long non-coding RNA (lncRNA) IDI2-AS1/microRNA-33b-5p (miR-33b-5p)/nuclear receptor-associated protein NR4A2 competitive endogenous RNA (ceRNA) regulatory network on acute myocardial infarction (AMI), and to verify whether IDI2-AS1 regulates NR4A2 through miR-33b-5p to affect the occurrence and development of myocardial infarction.Methods:The miRNA and mRNA expression chips related to myocardial infarction were obtained from gene expression omnibus (GEO), and the differential expression was analyzed. The upstream regulatory mechanism of NR4A2 was predicted using TargetScan database. Thirty-two male C57/BL6 mice were divided into Sham group, AMI model group, miR-33b-5p mimic group [miR-33b-5p mimic lentivirus (5×10 7 TU) was injected locally into the heart tissue during ligation] and miR-33b-5p inhibitor group [miR-33b-5p inhibitor lentivirus (5×10 7 TU) was injected locally into the heart tissue during ligation] according to random number table method, with 8 mice per group. Left ventricular end-diastolic diameter (LVEDD) and left ventricular end-systolic diameter (LVESD) were asseessed by echocardiography, left ventricular fractional shortening (LVFS) and left ventricular ejection fraction (LVEF) were calculated. After the last weighing, the anesthetized mice were sacrificed and the heart tissues were taken. Masson staining of the heart tissues was observed under light microscope, myocardial collagen volume fraction (CVF) and infarct size were calculated. Cardiomyocytes of SPF grade SD rats were collected. They were divided into normal control group (control group), ischemia-hypoxia model group, miR-33b-5p mimic transfection group (miR-33b-5p mimic transfection group before ischemia and hypoxia treatment) and miR-33b-5p inhibitor transfection group (miR-33b-5p inhibitor transfection group before ischemia and hypoxia treatment). The activity of caspase-3/7 in cardiomyocytes was measured. The levels of interleukins (IL-1β, IL-6) and tumor necrosis factor-α (TNF-α) were detected by enzyme-linked immunosorbent assay (ELISA). The levels of malondialdehyde (MDA), superoxide dismutase (SOD), creatine kinase (CK), MB isoenzyme of creatine kinase (CK-MB) and lactate dehydrogenase (LDH) were detected by colorimetry. Real-time quantitative polymerase chain reaction (RT-qPCR) was used to detect the expression of apoptosis-related proteins Bax and Bcl-2, cytochrome C (Cyt C) and IDI2-AS1/miR-33b-5p/NR4A2 regulatory axis genes. Results:The myocardial infarction microarray analysis showed that NR4A2 expression was significantly up-regulated in myocardial infarction, with predicted upstream regulatory mechanisms indicating its possible influence through the IDI2-AS1/miR-33b-5p/NR4A2 regulatory axis. Echocardiographic detection showed that compared with AMI model group and miR-33b-5p inhibitor group, LVEF and LVFS in the heart tissue of mice in miR-33b-5p mimic group were significantly increased, while the levels of LVEDD, LVESD, CK, CK-MB and LDH were significantly decreased, with statistical significance. Light microscope showed myocardial fibrosis and myocardial infarction in AMI model group and miR-33b-5p inhibitor group. In the miR-33b-5p mimic group, the degree of myocardial fibrosis was decreased and the myocardial infarction size was significantly reduced. Compared with AMI model group and miR-33b-5p inhibitor group, the levels of MDA, IL-1β, IL-6, TNF-α and the expressions of Bax and Cyt C in the heart tissue of mice in miR-33b-5p mimic group were significantly decreased, while the levels of SOD and Bcl-2 expression were significantly increased, and the differences were statistically significant. The expressions of IDI2-AS1 and NR4A2 in the heart tissue of mice in miR-33b-5p mimic group were significantly lower than those in AMI model group and miR-33b-5p inhibitor group [IDI2-AS1 (2 -ΔΔCt): 1.96±0.08 vs. 2.73±0.08, 3.10±0.05, NR4A2 (2 -ΔΔCt): 2.36±0.07 vs. 3.16±0.08, 3.80±0.08, all P < 0.01]. The expression of miR-33b-5p was significantly higher than that of AMI model group and miR-33b-5p inhibitor group (2 -ΔΔCt: 0.88±0.07 vs. 0.57±0.07, 0.23±0.01, both P < 0.01). The cell experiment results showed that the caspase-3/7 activity of rat neonatal cardiomyocytes in the miR-33b-5p mimic transfection group was significantly lower than that in the ischemia-hypoxia model group and the miR-33b-5p inhibitor transfection group, suggesting that miR-33b-5p can significantly reduce the apoptosis level of the ischemia-hypoxia model. The levels of peroxidation and inflammation indexes, important genes of apoptosis pathway and the expression of IDI2-AS1/miR-33b-5p/NR4A2 regulatory axis of rat neonatal cardiomyocytes in all groups were consistent with the above. Conclusion:IDI2-AS1 can regulate NR4A2 through miR-33b-5p, thus affecting the occurrence and development of AMI.

Antibody drug conjugates(ADCs)are a new class of anti-tumor drugs in which linkers in the structure link cytotoxic drugs to monoclonal an-tibodies and release cytotoxic drugs to tumors.Disi-tamab vedotin(RC48)is a new antibody-drug con-jugate independently developed in China.It targets the HER2 protein on the surface of tumors,it has both antibody targeting and small molecule drug killing,and can accurately recognize and kill tumor cells.Compared with traditional HER2-targeted drugs,disitamab vedotin has a wider therapeutic window and less toxicity to normal tissues.Current-ly,disitamab vedotin for injection has been ap-proved by the National Medical Products Adminis-tration(NMPA)for use in patients with HER2 over-expression(HER2 immunohistochemical results of 2+or 3+)who have locally advanced or metastatic gastric cancer(including gastroesophageal junction adenocarcinoma)and have received at least two types of systemic chemotherapy.Additionally,it is indicated for patients with locally advanced or met-astatic urothelial carcinoma who have previously undergone platinum-containing chemotherapy and exhibit HER2 overexpression,specifically 2+or 3+immunohistochemical results.In this paper,we will review the structural characteristics,mechanism of action and clinical trials of disitamab vedotin and look forward to the clinical application prospects of this drug.

Antibody drug conjugates(ADCs)are a new class of anti-tumor drugs in which linkers in the structure link cytotoxic drugs to monoclonal an-tibodies and release cytotoxic drugs to tumors.Disi-tamab vedotin(RC48)is a new antibody-drug con-jugate independently developed in China.It targets the HER2 protein on the surface of tumors,it has both antibody targeting and small molecule drug killing,and can accurately recognize and kill tumor cells.Compared with traditional HER2-targeted drugs,disitamab vedotin has a wider therapeutic window and less toxicity to normal tissues.Current-ly,disitamab vedotin for injection has been ap-proved by the National Medical Products Adminis-tration(NMPA)for use in patients with HER2 over-expression(HER2 immunohistochemical results of 2+or 3+)who have locally advanced or metastatic gastric cancer(including gastroesophageal junction adenocarcinoma)and have received at least two types of systemic chemotherapy.Additionally,it is indicated for patients with locally advanced or met-astatic urothelial carcinoma who have previously undergone platinum-containing chemotherapy and exhibit HER2 overexpression,specifically 2+or 3+immunohistochemical results.In this paper,we will review the structural characteristics,mechanism of action and clinical trials of disitamab vedotin and look forward to the clinical application prospects of this drug.

Objective To compare and analyze the difference of cardiopulmonary reserve function of phlegm-dampness and peaceful constitutions. Methods A total of 102 cases were selected for the physi-cal examination in Air Service Department of Northern Theater Air Force Hospital,and divided into a phlegm-dampness constitution group of 50 and a peaceful constitution group of 52 according to their traditional Chinese medicine constitution. Both groups underwent the cardiopulmonary exercise testing (CPET),and the cardiopulmonary reserve function parameters such as the peak exercise load,peak metabolic equivalent,peak kilogram oxygen uptake,and peak oxygen pulse rate were compared be-tween the 2 groups. Results There was no significant difference between the 2 groups in age,as well as the history of smoking and drinking(P>0.05). Moreover,the average body mass index,neck circum-ference,heart rate reserve,and peak ventilation of the phlegm-dampness constitution group were signif-icantly higher than the peaceful constitution group,while the average peak exercise load,peak meta-bolic equivalent,peak heart rate,peak oxygen pulse,and peak kilogram oxygen uptake were signifi-cantly lower than the latter group(P<0.05). Conclusions Compared with the people of peaceful constitu-tion,those of phlegm-dampness have lower cardiopulmonary reserve function. Therefore,early physical intervention should be conducted to help them to get into a healthy lifestyle.

In atherosclerosis, chronic inflammatory processes in local diseased areas may lead to the accumulation of reactive oxygen species (ROS). In this study, we devised a highly sensitive H₂O₂-scavenging nano-bionic system loaded with probucol (RPP-PU), to treat atherosclerosis more effectively. The RPP material had high sensitivity to H₂O₂, and the response sensitivity could be reduced from 40 to 10 μmol/L which was close to the lowest concentration of H₂O₂ levels of the pathological environment. RPP-PU delayed the release and prolonged the duration of PU in vivo. In Apolipoprotein E deficient (ApoE^‒/‒) mice, RPP-PU effectively eliminated pathological ROS, reduced the level of lipids and related metabolic enzymes, and significantly decreased the area of vascular plaques and fibers. Our study demonstrated that the H₂O₂-scavenging nano-bionic system could scavenge the abundant ROS in the atherosclerosis lesion, thereby reducing the oxidative stress for treating atherosclerosis and thus achieve the therapeutic goals with atherosclerosis more desirably.