Respiratory syncytial virus(RSV)and the acute respiratory infections it induced have become a global key public health issue.However,the existing antiviral drugs are insufficient and have limitations such as drug resistance,limited applicability,and high toxic side effects,which urgently require the development of new therapeutic methods and drugs.As an important part of traditional medicine,traditional Chinese medicine(TCM)and its compound preparations have rich clinical accumulation and potential advantages in the field of antiviral therapy.Their unique targets and multi-link synergistic effects are highly compatible with the complex patho-genic network of RSV.The article aims to describe the infection pathway and key targets of RSV,summarize the progress and shortcom-ings of existing therapeutic methods,and systematically analyze the regulatory mechanism and drug potential of TCM and its compound preparations on different stages of viral infection,so as to provide reference for improving the prevention and control capabilities and de-veloping new antiviral drugs in China.

Respiratory syncytial virus(RSV)and the acute respiratory infections it induced have become a global key public health issue.However,the existing antiviral drugs are insufficient and have limitations such as drug resistance,limited applicability,and high toxic side effects,which urgently require the development of new therapeutic methods and drugs.As an important part of traditional medicine,traditional Chinese medicine(TCM)and its compound preparations have rich clinical accumulation and potential advantages in the field of antiviral therapy.Their unique targets and multi-link synergistic effects are highly compatible with the complex patho-genic network of RSV.The article aims to describe the infection pathway and key targets of RSV,summarize the progress and shortcom-ings of existing therapeutic methods,and systematically analyze the regulatory mechanism and drug potential of TCM and its compound preparations on different stages of viral infection,so as to provide reference for improving the prevention and control capabilities and de-veloping new antiviral drugs in China.

Objective To explore the effect of L-ascorbic acid(AA) for reducing the fibroblast cells induced by cobalt(co)cytotoxicity.Methods The experiment was divided into the blank control group,Co2+ group,Co2++AA group,cobalt nanoparticles(CoNPs) group,CoNPs+AA group and AA group.The AA pretreated for 1 h was performed in advance.The CCK8 assay was used to detect the CoNPs and Co2+ induced and AA-treated cytotoxicity.The fluorescence staining was used to measure the production of ROS in mitochondria.The real-time polymerase chain reaction(PCR) and Western blot were used to measure the expression of related protein and related molecular mRNA respectively.Moreover the cytochrome-C level in cytoplasml was detected.Results After treating the cells by CoNPs and Co2+,the cells appeared apoptosis.CoNPs could significantly induce ROS generation;the proapoptotic factors(caspases-3,and Bax) were significantly increased,while the anti-apoptotic factor Bcl-2 expression was decreased;cytochrome C and AIF expressions were up-regulated and released from mitochondria into the cytoplasm,after AA pretreatment,these changes caused by CoNPs were decreased.Conclusion AA can reduce the CoNPs caused cytotoxicity by reducing ROS generation and release,but can not weaken the Co2+ toxic effect.