This document targets digital human metabolic chamber platforms and specifies construction standards and testing protocols covering the full lifecycle of " build-test-operate." It encompasses chamber engineering and environmental control, digital platform and cybersecurity architecture, metabolic measurement and multimodal data acquisition, as well as quantitative system performance and data quality indicators with verifiable acceptance tests. By standardizing architecture, interfaces, and quality control, the specification enables multicenter data interoperability and harmonized quality management, providing high-quality, verifiable, and traceable infrastructure to support precision metabolism research and clinical translation in China.

Objective To explore the impact of nursing intervention based on the information-motivation-behavioral skill(IMB)model on the acceptance of disability in stroke patients.Methods A total of 92 patients with first-onset stroke who were hospitalized at the Affiliated Hospital of Hangzhou Normal University from July 2022 to December 2023 were selected and divided into experimental group(47 cases)and control group(45 cases)according to the random number table method.The control group received routine care,while the experimental group received nursing intervention based on the IMB model in addition to routine care.The acceptance of disability and depression levels of two groups were compared.Results After the intervention,the acceptance of disability in experimental group was significantly higher than that in control group and increased over time(P＜0.05).The degree of depression in experimental group was significantly lower than that in control group and decreased over time(P＜0.05).Conclusion Nursing intervention based on the IMB model can enhance the acceptance of disability among stroke patients,reduce the degree of depression,and improve disease prognosis,demonstrating good clinical practical value.

This document targets digital human metabolic chamber platforms and specifies construction standards and testing protocols covering the full lifecycle of " build-test-operate." It encompasses chamber engineering and environmental control, digital platform and cybersecurity architecture, metabolic measurement and multimodal data acquisition, as well as quantitative system performance and data quality indicators with verifiable acceptance tests. By standardizing architecture, interfaces, and quality control, the specification enables multicenter data interoperability and harmonized quality management, providing high-quality, verifiable, and traceable infrastructure to support precision metabolism research and clinical translation in China.

Objective To explore the impact of nursing intervention based on the information-motivation-behavioral skill(IMB)model on the acceptance of disability in stroke patients.Methods A total of 92 patients with first-onset stroke who were hospitalized at the Affiliated Hospital of Hangzhou Normal University from July 2022 to December 2023 were selected and divided into experimental group(47 cases)and control group(45 cases)according to the random number table method.The control group received routine care,while the experimental group received nursing intervention based on the IMB model in addition to routine care.The acceptance of disability and depression levels of two groups were compared.Results After the intervention,the acceptance of disability in experimental group was significantly higher than that in control group and increased over time(P＜0.05).The degree of depression in experimental group was significantly lower than that in control group and decreased over time(P＜0.05).Conclusion Nursing intervention based on the IMB model can enhance the acceptance of disability among stroke patients,reduce the degree of depression,and improve disease prognosis,demonstrating good clinical practical value.

Objective:To evaluate the clinical value of multi-track specialized nursing based on the transtheoretical model (TTM) in the care of patients with myasthenia gravis (MG) .Methods:A total of 108 patients with MG admitted to Shanxi Provincial People's Hospital from September 2022 to September 2023 were selected by convenience sampling and randomly divided into a control group and an observation group ( n=54 each) using a random number table. The control group received routine nursing care, while the observation group received multi-track specialized nursing based on the TTM in addition to routine care. The intervention lasted for six months. Barthel Index, Connor-Davidson Resilience Scale (CD-RISC), General Self-Efficacy Scale (GSES), 36-item Short Form Health Survey (SF-36), and Fatigue Severity Scale (FSS) were measured before intervention, and at 3 and 6 months after intervention. Results:At both 3 and 6 months post-intervention, the observation group showed higher scores in Barthel Index, CD-RISC, GSES, and SF-36, and lower FSS scores compared to the control group. The differences were all statistically significant ( P<0.05) . Conclusions:The multi-track specialized nursing model based on the TTM can improve rehabilitation outcomes in patients with MG, enhance psychological resilience and self-efficacy, improve quality of life, and alleviate fatigue.

Objective:To evaluate the clinical value of multi-track specialized nursing based on the transtheoretical model (TTM) in the care of patients with myasthenia gravis (MG) .Methods:A total of 108 patients with MG admitted to Shanxi Provincial People's Hospital from September 2022 to September 2023 were selected by convenience sampling and randomly divided into a control group and an observation group ( n=54 each) using a random number table. The control group received routine nursing care, while the observation group received multi-track specialized nursing based on the TTM in addition to routine care. The intervention lasted for six months. Barthel Index, Connor-Davidson Resilience Scale (CD-RISC), General Self-Efficacy Scale (GSES), 36-item Short Form Health Survey (SF-36), and Fatigue Severity Scale (FSS) were measured before intervention, and at 3 and 6 months after intervention. Results:At both 3 and 6 months post-intervention, the observation group showed higher scores in Barthel Index, CD-RISC, GSES, and SF-36, and lower FSS scores compared to the control group. The differences were all statistically significant ( P<0.05) . Conclusions:The multi-track specialized nursing model based on the TTM can improve rehabilitation outcomes in patients with MG, enhance psychological resilience and self-efficacy, improve quality of life, and alleviate fatigue.

Objective:To investigate the mechanism of trigger point deactivation induced by pressing manipulation in a rat model and to explore its potential regulation of the inflammatory response through the extracellular signal-regulated kinase(ERK)/nuclear factor-κB(NF-κB)pathway. Methods:Fifty male Sprague-Dawley rats were randomly divided into a blank group,a model group,a pressing manipulation group,an ERK agonist group,and a pressing manipulation+ERK agonist group,with 10 rats in each group.Except for the blank group,rats in other groups were used to establish the trigger point rat model using the blunt blow combined with the eccentric exercise method.The pressing manipulation group underwent pressing manipulation intervention at the trigger points.The ERK agonist group received an injection of recombinant human epidermal growth factor via the tail vein.The pressing manipulation+ERK agonist group received interventions from both the pressing manipulation and ERK agonist groups.The pressure pain threshold(PPT)was measured by a mechanical pain threshold detector before and after the intervention.The histological changes were evaluated by hematoxylin-eosin staining after the intervention;the expression levels of ERK,phosphorylated ERK(p-ERK),NF-κB p65(p65),phosphorylated NF-κB p65(p-p65),and phosphorylated NF-κB inhibitor(p-IκB)were detected by Western blotting;the levels of interleukin(IL)-1β,IL-6,and tumor necrosis factor(TNF)-α were detected by enzyme-linked immunosorbent assay. Results:The PPT increased(P<0.05);the inflammatory cells disappeared;the ratios of p-ERK/ERK,p-p65/p65,and p-IκB/β-actin,also the levels of IL-1β,IL-6,and TNF-α all decreased in the pressing manipulation group after the intervention compared with the model group(P<0.05).The PPT decreased significantly(P<0.05),the inflammatory cell presence increased,and the ratios of p-ERK/ERK and p-p65/p65 were elevated(P<0.05);additionally,the levels of IL-6 and TNF-α were significantly higher in the pressing manipulation+ERK agonist group compared with the pressing manipulation group(P<0.05).The PPT was significantly lower(P<0.05),the inflammatory cell count was higher,the ratios of p-ERK/ERK and p-IκB/β-actin and the levels of IL-1β and TNF-α were significantly higher in the ERK agonist group compared with the pressing manipulation+ERK agonist group(P<0.05). Conclusion:Pressing manipulation can effectively alleviate inflammation and pain in trigger point model rats,potentially by inhibiting the ERK/NF-κB signaling pathway.

Background::Genetic variants of dopaminergic transcription factor-encoding genes are suggested to be Parkinson’s disease (PD) risk factors; however, no comprehensive analyses of these genes in patients with PD have been undertaken. Therefore, we aimed to genetically analyze 16 dopaminergic transcription factor genes in Chinese patients with PD.Methods::Whole-exome sequencing (WES) was performed using a Chinese cohort comprising 1917 unrelated patients with familial or sporadic early-onset PD and 1652 controls. Additionally, whole-genome sequencing (WGS) was performed using another Chinese cohort comprising 1962 unrelated patients with sporadic late-onset PD and 1279 controls.Results::We detected 308 rare and 208 rare protein-altering variants in the WES and WGS cohorts, respectively. Gene-based association analyses of rare variants suggested that MSX1 is enriched in sporadic late-onset PD. However, the significance did not pass the Bonferroni correction. Meanwhile, 72 and 1730 common variants were found in the WES and WGS cohorts, respectively. Unfortunately, single-variant logistic association analyses did not identify significant associations between common variants and PD. Conclusions::Variants of 16 typical dopaminergic transcription factors might not be major genetic risk factors for PD in Chinese patients. However, we highlight the complexity of PD and the need for extensive research elucidating its etiology.

Objective This paper summarizes and evaluates the existing animal models of Post-infectious irritable bowel syndrome(PI-IBS)based on the clinical diagnostic criteria and symptom characteristics of PI-IBS in Chinese and western medicine.The goal of this study is to seek out animal models with a high degree of clinical agreement between Chinese and western medicine,and to provide an accurate animal model for the prevention and treatment of PI-IBS in traditional Chinese medicine.Methods The animal strains,modelling methods,and mechanisms of the existing PI-IBS animal models were summarized based on the clinical diagnostic features and symptomatology of PI-IBS,as well as the degree of match was evaluated and the strengths and weaknesses of the preparation of animal models were analyzed,by reviewing the articles related to PI-IBS animal experiments and the preparation of animal models.Results A thorough analysis discovered that Campylobacter jejuni infection model,Trichinella infection model,and liver depression and spleen deficiency syndrome in models of disease and symptom combination showed a comparatively high level of clinical agreement between Chinese and western medicine.Conclusion The existing PI-IBS models had the high degree of coincidence with western medicine,Chinese medicine four diagnoses and syndrome are generally less embodied and collected in the modeling process.The primary symptoms in Chinese medicine diagnosis are primarily based on the observation of animal apparent behaviors,while the observation of the sub-syndrome manifestations is relatively less,which results in failure of judgment of TCM syndrome types.Therefore,it is still necessary to further standardize the criteria for evaluating symptoms and the techniques for identifying disease-syndrome combination animal models.PI-IBS model with the syndrome-clinical characteristics in Chinese and western medicine has significant application value and prospects in the future.The multifactorial composite method of western medicine pathological injury+Chinese medicine etiological stimulation can establish a PI-IBS model with a higher degree of coincidence,which can provide theoretical support for the study of the pathogenesis of PI-IBS,the difference of syndrome,and the prevention and treatment with integrative Chinese and western medicine.It is crucial to guide the clinical diagnosis and treatment of PI-IBS.

Using the liquid chromatography-tandem mass spectrometry (LC-MS/MS) to determine the plasma level of Lyso-GL3 in patients with Fabry disease and to analyze the clinical application of the method.