To develop and validate a model for predicting intensive care unit (ICU) mortality risk in patients with malignant tumors complicated by acute respiratory failure (ARF) based on an explainable machine learning framework.

ObjectiveTo explore the current situation， causes， and countermeasures of medical disputes in a tertiary A comprehensive hospital in Xi’an from 2018 to 2023. MethodsA total of 804 medical dispute cases that had been processed in a tertiary A hospital in Xi’an from January 2018 to December 2023 were collected. The occurrence， current characteristics， and cause distribution of medical disputes were retrospectively analyzed. ResultsThe cumulative incidence rate of medical disputes was 4.408/100，000， and the compensation rate was 31.59% （254/804）. The incidence and compensation rate of medical disputes showed an overall increasing trend year by year from 2018 to 2023 （P<0.05）. The proportion of deceased patients in dispute cases in the past 6 years showed an increasing trend annually （χ²=30.396， P=0.011）. Medical dispute cases mainly came from hospitalized patients （65.17%） and patients undergoing surgical treatment （55.35%）， and the top five departments with medical dispute cases were the hepatobiliary surgery department， cardiovascular medicine department， obstetrics and gynecology department， emergency department， and gastroenterology department. The number of in-hospital mediations showed a decreasing trend annually， while the number of people’s mediations showed an increasing trend annually from 2018 to 2023 （χ²=34.523， P<0.001）. The top five causes of medical disputes in this hospital were surgical/operational complications （29.10%）， disease condition assessment/disease diagnosis （10.45%）， diagnosis and treatment plan （9.45%）， diagnosis and treatment effectiveness （8.83%）， and medical insurance reimbursement （7.59%）. ConclusionThe number of medical disputes and compensation cases in comprehensive hospitals is increasing annually， and high-risk departments such as the surgical department and emergency department need to be given high attention. The handling of medical disputes has shown diversification， with people’s mediation gradually taking the dominant position. The causes of medical disputes mainly concentrated on diagnosis and treatment technology， doctor-patient communication， medical insurance， and other aspects. It is necessary to continuously improve the level of clinical diagnosis and treatment technology， strengthen doctor-patient communication， improve the medical insurance system， strengthen hospital connotation management， alleviate doctor-patient conflicts， and builds a harmonious doctor-patient relationship.

Metachromatic leukodystrophy (MLD) is an inherited disease caused by a deficiency of the enzyme arylsulfatase A (ARSA). Lentivirus-modified autologous hematopoietic stem cell gene therapy (HSCGT) has recently been approved for clinical use in pre and early symptomatic children with MLD to increase ARSA activity. Unfortunately, this advanced therapy is not available for most patients with MLD who have progressed to more advanced symptomatic stages at diagnosis. Patients with late-onset juvenile MLD typically present with a slower neurological progression of symptoms and represent a significant burden to the economy and healthcare system, whereas those with early onset infantile MLD die within a few years of symptom onset. We conducted a pilot study to determine the safety and benefit of HSCGT in patients with postsymptomatic juvenile MLD and report preliminary results. The safety profile of HSCGT was favorable in this long-term follow-up over 9 years. The most common adverse events (AEs) within 2 months of HSCGT were related to busulfan conditioning, and all AEs resolved. No HSCGT-related AEs and no evidence of distorted hematopoietic differentiation during long-term follow-up for up to 9.6 years. Importantly, to date, patients have maintained remarkably improved ARSA activity with a stable disease state, including increased Functional Independence Measure (FIM) score and decreased magnetic resonance imaging (MRI) lesion score. This long-term follow-up pilot study suggests that HSCGT is safe and provides clinical benefit to patients with postsymptomatic juvenile MLD.
Humans ; Leukodystrophy, Metachromatic/genetics* ; Pilot Projects ; Genetic Therapy/methods* ; Hematopoietic Stem Cell Transplantation ; Male ; Follow-Up Studies ; Female ; Lentivirus/genetics* ; Child ; Child, Preschool ; Hematopoietic Stem Cells/metabolism* ; Cerebroside-Sulfatase/metabolism* ; Adolescent

Marine work environment is associated with unique risk factors,such as high salinity,high humidity,noise and vibration,and chemical pollution.Males make up the majority of marine workers.Prolonged exposure to these environmental factors may have adverse effects on male fertility,resulting in sperm quality reduction,endocrine disorder,and reproductive organ damage.Therefore,the potential harm of marine work environment to male reproductive health deserves attention.This review focused on the key exposure factors in marine work environment,and systematically explored the factors affecting male fertility,mechanisms,and physiological pathways.The aim is to provide scientific evidences to improve marine work environment,develop protective measures,and safeguard the reproductive health of workers,while also offering guidance for future research in this field.

Objective To evaluate the types and imaging features of early and late complications after liver transplantation.Methods A retro-spective analysis was conducted on the complications imaging data from 124 patients after liver transplantation.The CT and MRI characteristics of these complications was analyzed,categorized based on their occurrence time and type.Results The postoperative complications in the total 124 patients included vascular complication in 85 patients,biliary complication in 78 patients,and parenchy-mal complication in 21 patients.Additionally,the simple and complex complications were exhibited in 43 and 81 patients.Early vas-cular complication accounted for 69.8％,especially 21 cases(91.3％)were with early hepatic artery thrombosis.Bile leakage in 9 cases occurred in the early postoperative period,whereas biliary stenosis in 43 cases and biliary stones in 23 cases were observed in the late postoperative period.All ischemic infarction in 13 cases occurred in the early postoperative period.Among the 10 cases of liver abscess,8 cases were secondary to ischemic necrosis of liver parenchyma,and other 2 cases were secondary to suppurative cholangitis.Addi-tionally,6 cases of liver tumor recurrence were all detected in the late postoperative period.Conclusion The types of complications occurring after liver transplantation vary over time,and CT and MRI can be used to detect and evaluate postoperative complications.

Objective To explore the epidemiological characteristics and cost structure of hospitalized patients with acute exacerbation of chronic obstructive pulmonary disease(AECOPD)in a tertiary comprehensive hospital in Xi'an from 2014 to 2023.Methods The first page information of AECOPD inpatients in a hospital from January 2014 to December 2023 was col-lected(n=6 748),and the distribution of their demographic characteristics and clinical characteristics as well as the composition of hospitalization expenses were retrospectively analyzed,and the structural change degree was used to analyze the structural change of hospitalization expenses in different years.Results AECOPD patients diagnosed and hospitalized accounted for 4.81 ‰ of all hospitalized patients in this institution from 2014-2023,the proportion of AECOPD inpatients showed a continuous downward trend during 10 years(x2=1 083.351,P＜0.001);The distribution of gender,occupation,medical payment meth-ods,and sources of AECOPD inpatients in different years showed statistical differences(P＜0.05);There is a statistical differ-ence in the seasonal distribution of AECOPD inpatients in different years(x2=157.328,P＜0.001);There is a statistical difference in the average length of hospital stay among AECOPD inpatients in different years(x2=83.463,P＜0.001);There were statistically significant differences in the proportion of hospitalizations,admission conditions,comorbidities or complications,history of drug allergies,and readmission plans among AECOPD patients in different years(P＜0.05);There is a statistical difference in the outcomes of hospitalized AECOPD patients in different years(x2=153.367,P＜0.001);The average hospital-ization cost of AECOPD patients gradually increased from 2014-2016,and gradually decreased from 2016-2023(P＜0.01);The hospitalization expenses of AECOPD patients in different years are mainly based on drug expenses and diagnostic expenses;The overall degree of structural change(DSV)of average hospitalization expenses for AECOPD patients from 2014-2022 was 41.62％,with the highest DSV of hospitalization expenses in 2012-2013(22.69％)and the lowest DSV in 2017-2018(4.23％);The proportion of drug expenses in the average hospitalization cost of AECOPD patients continued to decline,while diagnostic expenses,hygiene material expenses,medical service expenses,and nursing expenses showed an overall increasing trend from 2014-2023.Conclusion The proportion and mortality rate of hospitalized AECOPD patients have shown an overall downward trend in the past 10 years;The proportion of hospitalized AECOPD patients in the age group of 70-79 is the highest,mainly distributed in winter;The overall average hospitalization cost over the past 10 years has shown a downward trend,with drug and diagnostic expenses as the main components.Drug expenses have been decreasing year by year,while diagnostic expen-ses have been increasing year by year.This indicates that medical policy reform plays a significant role in optimizing the hospitali-zation cost structure and controlling unreasonable cost growth.

Glioma is a common primary intracra-nial tumor.Malignant glioma has a high mortality rate and an inferior prognosis.Despite various ther-apeutic interventions,the overall survival rate is still meager.Sesquiterpene lactone is a kind of natu-ral product containing α-methylene-γ-lactone,which has strong anti-tumor activity.In recent years,there have been many reports on the anti-gli-oma effect of sesquiterpene lactone compounds,such as ACT001,which is a structural modification of sesquiterpene lactone(Parthenolide)and has en-tered the clinical trial stage as a potential antican-cer drug.This paper reviews the activity and mecha-nism of sesquiterpene lactones with anti-glioma ef-fects,which have been studied in recent.years.

Objective:To explore the effect of identifying pelvic lymph nodes by different injection of indocyanine green (ICG) during fluorescent laparoscopic radical cystectomy.Methods:The data of 54 male bladder cancer patients admitted to the First Hospital of Shanxi Medical University from September 2021 to September 2022 were analyzed. Preoperative cystoscopic biopsy all revealed a pathological diagnosis of urothelial carcinoma. All patients underwent fluorescent laparoscopic radical cystectomy plus pelvic lymph node dissection.They were divided into 3 groups according to the annotated route: 6 in the medial malleolus injection group, 68(61, 73) years; 4 in the perineal injection group, 67(59, 74) years; 44 in the medial malleolus and perineum, 64(45, 78) in the combined injection group. Under general anesthesia, patients were placed in the supine position with 0.3 to 0.5 ml of subcutaneous ICG solution (2.5 mg/ml) injected from 1.0 to 1.5 h before surgery. In the medial malleolus injection group, 0.3 to 0.5 ml (0.75 mg/place) was injected at 2 cm above the medial malleolus and 0.3 to 0.5 ml (0.75 mg/place) at two symmetrical sites above the anus. Intraoperatively, 4K fluorescent laparoscopy was used to observe the color development of the injection site and lymphatic vessels in vitro, and then to observe the development of pelvic lymph nodes in vivo.Results:In 3 groups, inguinal lymph nodes were developed about 30 min after ICG injection, and 1 h later, the longest (4.5±0.3) h. The external iliac and common iliac lymph nodes, the obturatorius lymph nodes in the perineal injection group, and the inguinal, obturatorius, external iliac, internal, anterior sacral, and common iliac lymph nodes in the medial malleolar and perineal injection group. In this study, in 54 cases, the postoperative pathological examination confirmed that the removed fluorescently labeled tissue was lymph node tissue, and the lymph node detection rate was 100%. The postoperative stage was pT 2, 14, 32 pT 3 and 8 pT 4; 4 pNx, 44 pN 0, 5 pN 1and 1 pN 2. In the medial malleolar injection group, 2 patients staged pT 2, 3 stage pT 3, 1 stage pT 4; 1stage pNx stage, 4 stage pN 0 and 1 stage pN 1 stage. In the perineal injection group, 4 patients had postoperative pathological stage pT 3N 0; in the medial malleolar and perineal injection group, 12 pT 2, 25 and 7 pT 3; 3 pNx, 36 pN 0, 4 pN 1 and 1pN 2. Conclusions:ICG injection through the perineum and subcutaneous labeling of pelvic lymph nodes, and the simple malleolus or perineal injection can not completely develop the pelvic lymph nodes. The combined injection could fully develop the pelvic lymph nodes, which may accurately guide the operator to locate, identify and remove pelvic lymph nodes.

Osteoporosis (OP) is a systemic skeletal disease that primarily affects the elderly population, which greatly increases the risk of fractures. Here we report that Kindlin-2 expression in adipose tissue increases during aging and high-fat diet fed and is accompanied by decreased bone mass. Kindlin-2 specific deletion (K2KO) controlled by Adipoq-Cre mice or adipose tissue-targeting AAV (AAV-Rec2-CasRx-sgK2) significantly increases bone mass. Mechanistically, Kindlin-2 promotes peroxisome proliferator-activated receptor gamma (PPARγ) activation and downstream fatty acid binding protein 4 (FABP4) expression through stabilizing fatty acid synthase (FAS), and increased FABP4 inhibits insulin expression and decreases bone mass. Kindlin-2 inhibition results in accelerated FAS degradation, decreased PPARγ activation and FABP4 expression, and therefore increased insulin expression and bone mass. Interestingly, we find that FABP4 is increased while insulin is decreased in serum of OP patients. Increased FABP4 expression through PPARγ activation by rosiglitazone reverses the high bone mass phenotype of K2KO mice. Inhibition of FAS by C75 phenocopies the high bone mass phenotype of K2KO mice. Collectively, our study establishes a novel Kindlin-2/FAS/PPARγ/FABP4/insulin axis in adipose tissue modulating bone mass and strongly indicates that FAS and Kindlin-2 are new potential targets and C75 or AAV-Rec2-CasRx-sgK2 treatment are potential strategies for OP treatment.

The research on rare diseases in China started relatively late, with scattered research resources and weak data foundation in epidemiology, diagnosis and treatment, and medication, which hinders its research progress. The rare disease data system is the foundation of rare disease research, and the ethical constraint on rare disease data collection is not only the protection of rare disease population, but also the need for the safety and quality of rare disease data. By analyzing and prospecting the current status of the construction of rare disease data systems, including the data of epidemiology, clinical diagnosis and treatment, drug trials, and follow-up to provide reference for the improvement of rare disease data systems. This paper explored the ethical issues to be followed in the process of rare disease data collection from the perspectives of justice, no harm, respect, sharing, and legalization, so as to improve the standardization of rare disease data collection and the understanding of data ethical review.