Objective To explore a nomogram model based on clinical characteristics and immune indicators for predicting the efficacy of tyrosine kinase inhibitor(TKI)against chronic myeloid leukemia(CML).Methods Clinical data was retrospectively collected from 100 patients with CML treated with TKI between January 2021 and January 2023 in Tangshan Gongren Hospital.Patients were divided into the best response and warning/treatment failure groups according to therapeutic efficacy.Factors affecting therapeutic efficacy were analyzed using logistic regression analysis,and a nomogram model was constructed.Results The best response and warning/treatment failure groups showed significant differences in red blood cell distribution width(RDW),platelet count(PLT),ELTS score,Th 1/CD4+,Treg/CD4+ratio,white blood cell count,and absolute value of natural killer cells(P＜0.05).Logistic regression confirmed that the above indicators were influencing factors(P＜0.05),indicating that the model was meaningful,and had a high goodness of fit as well as high predictive value.Conclusion The nomogram model constructed based on RDW,PLT,and other factors can effectively predict the thera-peutic efficacy of AKI in treating CML.

With the widespread application of information technology in the field of Traditional Chinese Medicine(TCM),the data in the TCM domain is becoming increasingly rich.The emergence of data-driven research in TCM has utilized knowledge graphs for the management,analysis,and presentation of TCM data,becoming a crucial method for the inheritance and innovation of Traditional Chinese Medicine.Therefore,reasoning based on TCM knowledge graphs has become a hot topic in contemporary TCM research.In order to better utilize knowledge graph reasoning technology to promote innovation and development in TCM,this article,after providing an overview of TCM knowledge graph reasoning,focuses on the development of knowledge graph reasoning in TCM and its applications from three perspectives:logic rule-based,distributed representation,and neural network-based methods.Finally,the article summarizes the advantages and disadvantages of knowledge reasoning methods and provides prospects for research and applications in TCM knowledge graph reasoning,including studies on interpretability,temporal knowledge graphs,and the fusion of multimodal information.

To analyze the prevalence, drug resistance and whole genome characteristics of Mycoplasma pneumoniae (MP) in respiratory throat swab samples of hospitalized children with pneumonia in Suzhou City from 2023 to 2024. Throat swab samples of hospitalized children aged 0-14 years old with pneumonia in Suzhou were collected from September 2023 to September 2024. Real-time fluorenscence quantitative PCR technology was used to detect MP nucleic acid. The results showed that the positive rate of MP in 3 235 samples was 22.44% (726/3 235), with a rate of 55.00% in week 47 of 2023. The positive rate of MP increased with age ( χ2=45.842, P<0.001). The study selected MP nucleic acid test positive samples from week 20 (5.13-5.19) to week 23 (6.3-6.9) of 2024 for isolation, culture and resistance phenotype detection. About 31 MP strains were successfully isolated and cultured, all of which were resistant to macrolides. The next-generation sequencing technology and nanopore sequencing technology were used for genome sequencing. All 31 strains carried the A2063G mutation, with the main prevalent genotype being the P1-1, and the main mlST type being the ST3. Despite the overall genomic similarity between strains being over 99%, there were significant differences between the P1-1 and P1-2 strains in the P1 gene region. In summary, from 2023 to 2024, the main MP type prevalent in Suzhou City is the P1-1 genotype. All isolated MP strains carry an A2063G resistance site mutation and are resistant to macrolides, requiring continuous monitoring and further research.

With the widespread application of information technology in the field of Traditional Chinese Medicine(TCM),the data in the TCM domain is becoming increasingly rich.The emergence of data-driven research in TCM has utilized knowledge graphs for the management,analysis,and presentation of TCM data,becoming a crucial method for the inheritance and innovation of Traditional Chinese Medicine.Therefore,reasoning based on TCM knowledge graphs has become a hot topic in contemporary TCM research.In order to better utilize knowledge graph reasoning technology to promote innovation and development in TCM,this article,after providing an overview of TCM knowledge graph reasoning,focuses on the development of knowledge graph reasoning in TCM and its applications from three perspectives:logic rule-based,distributed representation,and neural network-based methods.Finally,the article summarizes the advantages and disadvantages of knowledge reasoning methods and provides prospects for research and applications in TCM knowledge graph reasoning,including studies on interpretability,temporal knowledge graphs,and the fusion of multimodal information.

Objectives:To explore the effects of guttering on the lateral mass technique in enlarging the cross-sectional area of the spinal canal after unilateral open-door cervical laminoplasty.Methods:A retro-spective analysis was conducted on the clinical data of patients with cervical ossification of posterior longitudi-nal ligament treated with unilateral open-door cervical laminoplasty between February 2021 and February 2024.37 patients who underwent the conventional technique were included in the conventional group;31 pa-tients undergone guttering on the lateral mass technique were included in the lateral mass group.The opera-tive time,intraoperative blood loss,postoperative drainage volume,and incidence of complications were compared between the two groups.At preoperation,immediately after surgery,2 months after surgery,and the final follow-up,the neck pain was evaluated using the visual analog scale(VAS),the cervical function was assessed with the neck disability index(NDI),and the neurological function was appraised with the Japanese Orthopaedic Association(JOA)score.C2-7 Cobb angle,C2-7 sagittal vertical axis(SVA),and T1 slope were measured on the cervical lateral X-rays to eliminate the possibilities of guttering on the lateral mass affecting cervical stability and cervical curvature;The spinal canal area was measured on the CT cross-sectional image and the percentage increase in spinal canal area was compared between the two groups.Results:All the pa-tients underwent the surgery successfully.No serious complications such as spinal cord injury or infection oc-curred.There were no significant differences in operation time,intraoperative blood loss,postoperative drainage volume between the two groups(P＞0.05).The neck pain VAS scores,NDI and JOA scores in both groups showed significant improvement immediately after surgery,2 months after surgery,and at final follow-up(P＜0.05).There were no differences in neck pain VAS scores and NDI between the two groups at preoperation,immediately after surgery,2 months after surgery and final follow-up(P＞0.05).There was no statistical differ-ence in preoperative JOA scores between the two groups(P＞0.05),but there were statistically significant differ-ences in JOA scores between the two groups immediately after surgery,2 months after surgery,and at final follow-up(P＜0.05).There were no statistical differences in C2-7 Cobb angle,C2-7 SVA,and T1 slope in both groups immediately after surgery,at 2 months after surgery,and at final follow-up,comparing with those before operation(P＞0.05).No significant differences were found between the two groups in C2-7 Cobb angle,C2-7 SVA,and T1 slope at preoperation,immediately after surgery,at 2-month after surgery,and at the fi-nal follow-up(P＞0.05).The average cross-sectional area of the spinal canal in both groups showed significant improvement immediately after surgery,at 2-month after surgery,and at final follow-up(P＜0.05).There was no statistical difference in the preoperative average cross-sectional area of the spinal canal between the two groups(P＞0.05).However,the average cross-sectional area of the spinal canal in the lateral mass group imme-diately after surgery,at 2-month after surgery,and at the final follow-up was larger than that in the conven-tional group,and the differences were statistically significant(P＜0.05).Conclusions:Compared with the con-ventional unilateral open-door cervical laminoplasty,guttering on the lateral mass technique can significantly expand the cross-sectional area of the spinal canal and improve postoperative neurological function recovery.

To analyze the prevalence, drug resistance and whole genome characteristics of Mycoplasma pneumoniae (MP) in respiratory throat swab samples of hospitalized children with pneumonia in Suzhou City from 2023 to 2024. Throat swab samples of hospitalized children aged 0-14 years old with pneumonia in Suzhou were collected from September 2023 to September 2024. Real-time fluorenscence quantitative PCR technology was used to detect MP nucleic acid. The results showed that the positive rate of MP in 3 235 samples was 22.44% (726/3 235), with a rate of 55.00% in week 47 of 2023. The positive rate of MP increased with age ( χ2=45.842, P<0.001). The study selected MP nucleic acid test positive samples from week 20 (5.13-5.19) to week 23 (6.3-6.9) of 2024 for isolation, culture and resistance phenotype detection. About 31 MP strains were successfully isolated and cultured, all of which were resistant to macrolides. The next-generation sequencing technology and nanopore sequencing technology were used for genome sequencing. All 31 strains carried the A2063G mutation, with the main prevalent genotype being the P1-1, and the main mlST type being the ST3. Despite the overall genomic similarity between strains being over 99%, there were significant differences between the P1-1 and P1-2 strains in the P1 gene region. In summary, from 2023 to 2024, the main MP type prevalent in Suzhou City is the P1-1 genotype. All isolated MP strains carry an A2063G resistance site mutation and are resistant to macrolides, requiring continuous monitoring and further research.

Objective To explore the clinical efficacy of Liuwei Dihuang Capsules for type 2 diabetes mellitus(T2DM)with yin deficiency syndrome and the effects on intestinal flora and inflammatory factors.Methods Totally 60 patients of T2DM with yin deficiency syndrome in Dongzhimen Hospital of Beijing University of Chinese Medicine from September 2022 to June 2023 were selected as the study objects,and were divided into control group and observation group according to the method of block randomization,with 30 cases in each group.Both groups received basic treatment.The control group was given a simulated agent of Liuwei Dihuang Capsules,while the observation group was given Liuwei Dihuang Capsules.The treatment course for both groups was 4 weeks.Clinical efficacy,blood glucose levels[fasting plasma glucose(FPG),2-hour postprandial plasma glucose(2 hPG),glycated albumin(GA)],serum insulin levels[fasting insulin(FINS)and insulin resistance index(HOMA-IR)],changes in gut microbiota,and serum inflammatory cytokine levels[interleukin(IL)-6,tumor necrosis factor(TNF)-α]of both groups were compared.Results The total effective rate of the observation group(76.67%)was better than that of the control group(50.00%)(P<0.05).Compared with before treatment,the FPG,2 hPG,GA,FINS and HOMA-IR decreased in the observation group,while the FPG,2 hPG and FINS decreased in the control group(P<0.05);after treatment,the Shannon index of the observation group increased after treatment(P<0.05),and the diversity of the microbiota increased;the abundance of the microbial communities such as Coprococcus 3,Cutibacterium,Pseudomonas,Faecalibaculum,Dubosiella and Mucispirillum significantly increased(P<0.05);the abundance of Sphingomonas,Corynebacterium 1,Ileibacterium,Ruminiclostridium and other microbiota communities significantly decreased(P<0.05).Compared with before treatment,the levels of IL-6 and TNF-α in both groups were significantly reduced after treatment(P<0.01,P<0.05).After treatment,the levels of IL-6 and TNF-α in the observation group were significantly lower than those in the control group(P<0.05).Conclusion Liuwei Dihuang Capsules can effectively reduce blood glucose levels in patients of T2DM with yin deficiency syndrome,improve insulin resistance,increase gut microbiota diversity,increase beneficial bacterial abundance,reduce harmful bacterial abundance,and alleviate inflammatory cytokine levels.

Objective:To examine the clinical effect of the enlarged anterior cervical intervertebral cone-shape decompression and fusion(EACDF) for treating degenerative cervical kyphosis (DCK).Methods:This study is a retrospective case series research. From September 2018 to September 2023, the data of 51 patients with DCK who underwent EACDF at Department of Orthopaedics, the Second Affiliated Hospital, Naval Medical University were analyzed retrospectively. Among the 51 patients, there were 28 males and 23 females, with an age of (61.6±9.8) years old (range:39 to 74 years), and an body mass index of (25.9±2.7) kg/m 2 (range:20.7 to 31.7 kg/m 2). Patients underwent EACDF with expanded decompression by distracted intervertebral space, partial resections of posterior edge of vertebral body and uncinate vertebral joint. The operation duration, blood loss and length of hospital stay were recorded. The visual analog scale (VAS) of neck pain and arm pain, cervical disability index (NDI), and modified Japanese Orthopaedic Association (mJOA) score were recorded in patients before and immediately after surgery, as well as at follow-up. Imaging parameters such as C 2-7 Cobb angle of cervical global curvature, Cobb angle at the operative segment, C 2-7 sagittal vertical axis (C 2-7 SVA), T 1 slope and the height of operative segment were measured. The fusion rate and surgical complications of two groups were recorded. Fourty-five patients who underwent anterior cervical corpectomy with fusion (ACCF) during the same period were included to compare the effectiveness of deformity correction between the two groups. Repeated measures ANOVA was used for intra group data comparison and Dunnett- t test was used for pairwise comparison, and mixed design ANOVA was used for inter group data comparison. Results:All patients were successfully completed the operation. The follow-up period after surgery was (40.4±13.4) months (range:12 to 72 months). The neck pain and arm pain VAS, NDI and JOA in the two groups immediately after surgery, at 2 months, 12 months after surgery, and the final follow-up were significantly improved compared with those before operation (all P<0.05). In both groups, postoperative the C 2-7 Cobb angle, Cobb angle at the operative segment, C 2-7 SVA, T 1 slope, and height of operative segment were significantly improved immediately after surgery, at 2 months, 12 months, and the final follow-up (all P<0.05). The C 2-7 Cobb angle, Cobb angle at the operative segment, and height of operative segment immediately after surgery, at 2 months, 12 months, and the final follow-up in the EACDF group were significantly higher than those in the ACCF group (all P<0.05). There were no significant differences in C 2-7 SVA and T 1 slope between the two groups(all P>0.05). At the final follow-up, the angle of every intervertebral space correction in the EACDF group was (9.3±1.6) °(range:6.5° to 12.3°), while in the ACCF group was (3.1±1.8) °(range:1.2° to 5.6°), with a significant difference between the two groups ( P<0.05). Patients at the both groups got bone graft fusion at the final follow-up. Conclusions:The clinical effect of EACDF for treating DCK is satisfactory. EACDF maybe superior to ACCF in restoring intervertebral height, correcting and maintaining cervical curvature.

Objective To explore a nomogram model based on clinical characteristics and immune indicators for predicting the efficacy of tyrosine kinase inhibitor(TKI)against chronic myeloid leukemia(CML).Methods Clinical data was retrospectively collected from 100 patients with CML treated with TKI between January 2021 and January 2023 in Tangshan Gongren Hospital.Patients were divided into the best response and warning/treatment failure groups according to therapeutic efficacy.Factors affecting therapeutic efficacy were analyzed using logistic regression analysis,and a nomogram model was constructed.Results The best response and warning/treatment failure groups showed significant differences in red blood cell distribution width(RDW),platelet count(PLT),ELTS score,Th 1/CD4+,Treg/CD4+ratio,white blood cell count,and absolute value of natural killer cells(P＜0.05).Logistic regression confirmed that the above indicators were influencing factors(P＜0.05),indicating that the model was meaningful,and had a high goodness of fit as well as high predictive value.Conclusion The nomogram model constructed based on RDW,PLT,and other factors can effectively predict the thera-peutic efficacy of AKI in treating CML.

Objectives:To explore the effects of guttering on the lateral mass technique in enlarging the cross-sectional area of the spinal canal after unilateral open-door cervical laminoplasty.Methods:A retro-spective analysis was conducted on the clinical data of patients with cervical ossification of posterior longitudi-nal ligament treated with unilateral open-door cervical laminoplasty between February 2021 and February 2024.37 patients who underwent the conventional technique were included in the conventional group;31 pa-tients undergone guttering on the lateral mass technique were included in the lateral mass group.The opera-tive time,intraoperative blood loss,postoperative drainage volume,and incidence of complications were compared between the two groups.At preoperation,immediately after surgery,2 months after surgery,and the final follow-up,the neck pain was evaluated using the visual analog scale(VAS),the cervical function was assessed with the neck disability index(NDI),and the neurological function was appraised with the Japanese Orthopaedic Association(JOA)score.C2-7 Cobb angle,C2-7 sagittal vertical axis(SVA),and T1 slope were measured on the cervical lateral X-rays to eliminate the possibilities of guttering on the lateral mass affecting cervical stability and cervical curvature;The spinal canal area was measured on the CT cross-sectional image and the percentage increase in spinal canal area was compared between the two groups.Results:All the pa-tients underwent the surgery successfully.No serious complications such as spinal cord injury or infection oc-curred.There were no significant differences in operation time,intraoperative blood loss,postoperative drainage volume between the two groups(P＞0.05).The neck pain VAS scores,NDI and JOA scores in both groups showed significant improvement immediately after surgery,2 months after surgery,and at final follow-up(P＜0.05).There were no differences in neck pain VAS scores and NDI between the two groups at preoperation,immediately after surgery,2 months after surgery and final follow-up(P＞0.05).There was no statistical differ-ence in preoperative JOA scores between the two groups(P＞0.05),but there were statistically significant differ-ences in JOA scores between the two groups immediately after surgery,2 months after surgery,and at final follow-up(P＜0.05).There were no statistical differences in C2-7 Cobb angle,C2-7 SVA,and T1 slope in both groups immediately after surgery,at 2 months after surgery,and at final follow-up,comparing with those before operation(P＞0.05).No significant differences were found between the two groups in C2-7 Cobb angle,C2-7 SVA,and T1 slope at preoperation,immediately after surgery,at 2-month after surgery,and at the fi-nal follow-up(P＞0.05).The average cross-sectional area of the spinal canal in both groups showed significant improvement immediately after surgery,at 2-month after surgery,and at final follow-up(P＜0.05).There was no statistical difference in the preoperative average cross-sectional area of the spinal canal between the two groups(P＞0.05).However,the average cross-sectional area of the spinal canal in the lateral mass group imme-diately after surgery,at 2-month after surgery,and at the final follow-up was larger than that in the conven-tional group,and the differences were statistically significant(P＜0.05).Conclusions:Compared with the con-ventional unilateral open-door cervical laminoplasty,guttering on the lateral mass technique can significantly expand the cross-sectional area of the spinal canal and improve postoperative neurological function recovery.