Growth arrest DNA damage-inducible protein 45 β (GADD45B) has been reported to be a regulatory factor for active DNA demethylation and is implicated in the modulation of synaptic plasticity and chronic stress-related psychopathological processes. However, its precise role and mechanism of action in stress susceptibility remain elusive. In this study, we found a significant reduction in GADD45B expression specifically in the ventral, but not the dorsal hippocampal CA1 (dCA1) of stress-susceptible mice. Furthermore, we demonstrated that GADD45B negatively regulates susceptibility to social stress and NMDA receptor-dependent long-term potentiation (LTP) in the ventral hippocampal CA1 (vCA1). Importantly, through pharmacological inhibition using the NMDA receptor antagonist MK801, we provided further evidence supporting the hypothesis that GADD45B potentially modulates susceptibility to social stress by influencing NMDA receptor-mediated LTP. Collectively, these results suggested that modulation of NMDA receptor-mediated synaptic plasticity is a pivotal mechanism underlying the regulation of susceptibility to social stress by GADD45B.
Animals ; Receptors, N-Methyl-D-Aspartate/antagonists & inhibitors* ; CA1 Region, Hippocampal/drug effects* ; Male ; Stress, Psychological/physiopathology* ; Mice ; Neuronal Plasticity/drug effects* ; Long-Term Potentiation/drug effects* ; Mice, Inbred C57BL ; Antigens, Differentiation/metabolism* ; Dizocilpine Maleate/pharmacology* ; Excitatory Amino Acid Antagonists/pharmacology* ; GADD45 Proteins

Objective:To analyze the correlation between serum inflammatory factor level and renal function injury in patients with sepsis.Methods:A total of 102 patients with sepsis diagnosed and treated in Shanghai Sixth People′s Hospital from May 2021 to May 2022 were retrospectively selected as the study objects. According to whether renal function injury occurred, they were divided into renal function injury group (37 cases) and no renal function injury group (65 cases). Multivariate Logistic regression was used to analyze the risk factors of renal function injury in patients with sepsis, and Pearson test was used to analyze the correlation between inflammatory factor and renal function injury in patients with sepsis. Receiver operating characteristic (ROC) curve was used to analyze the diagnostic value of procalcitonin (PCT), C-reactive protein (CRP) and interleukin 6 (IL-6) in patients with sepsis.Results:The results of multivariate Logistic regression analysis showed that Acute Physiological and Chronic Health Score System Ⅱ (APACHE Ⅱ) score, Sequential Organ Failure Assessment (SOFA) score, PCT, CRP, IL-6, blood urea nitrogen (BUN), 24 h urinary protein and serum creatinine (Scr) were risk factors for renal function injury in patients with sepsis ( P<0.05). The results of Pearson test showed that PCT, CRP and IL-6 were positively correlated with BUN, 24 h urinary protein and Scr of patients with sepsis ( r = 0.614, 0.622, 0.597, 0.672, 0.634, 0.611, 0.628, 0.589, 0.602, P<0.05). The results of ROC curve analysis showed that the area under the curve of PCT, CRP and IL-6 combined detection in patients diagnosed with sepsis was 0.879, and was higher than any single index ( P<0.05). Conclusions:The levels of PCT, CRP and IL-6 are increased in patients with sepsis, which has a certain impact on renal function, and has an important reference value for the evaluation of renal function injury in patients with sepsis.

Objective:To analyze the correlation between serum inflammatory factor level and renal function injury in patients with sepsis.Methods:A total of 102 patients with sepsis diagnosed and treated in Shanghai Sixth People′s Hospital from May 2021 to May 2022 were retrospectively selected as the study objects. According to whether renal function injury occurred, they were divided into renal function injury group (37 cases) and no renal function injury group (65 cases). Multivariate Logistic regression was used to analyze the risk factors of renal function injury in patients with sepsis, and Pearson test was used to analyze the correlation between inflammatory factor and renal function injury in patients with sepsis. Receiver operating characteristic (ROC) curve was used to analyze the diagnostic value of procalcitonin (PCT), C-reactive protein (CRP) and interleukin 6 (IL-6) in patients with sepsis.Results:The results of multivariate Logistic regression analysis showed that Acute Physiological and Chronic Health Score System Ⅱ (APACHE Ⅱ) score, Sequential Organ Failure Assessment (SOFA) score, PCT, CRP, IL-6, blood urea nitrogen (BUN), 24 h urinary protein and serum creatinine (Scr) were risk factors for renal function injury in patients with sepsis ( P<0.05). The results of Pearson test showed that PCT, CRP and IL-6 were positively correlated with BUN, 24 h urinary protein and Scr of patients with sepsis ( r = 0.614, 0.622, 0.597, 0.672, 0.634, 0.611, 0.628, 0.589, 0.602, P<0.05). The results of ROC curve analysis showed that the area under the curve of PCT, CRP and IL-6 combined detection in patients diagnosed with sepsis was 0.879, and was higher than any single index ( P<0.05). Conclusions:The levels of PCT, CRP and IL-6 are increased in patients with sepsis, which has a certain impact on renal function, and has an important reference value for the evaluation of renal function injury in patients with sepsis.

Objective:To investigate the association between congenital hypothyroidism (CH) and the adverse outcomes during hospitalization in very low birth weight infants (VLBWI).Methods:This prospective, multicenter observational cohort study was conducted based on the data from the Sino-northern Neonatal Network (SNN). Data of 5 818 VLBWI with birth weight <1 500 g and gestational age between 24-<37 weeks that were admitted to the 37 neonatal intensive care units from January 1 st, 2019 to December 31 st, 2022 were collected and analyzed. Thyroid function was first screened at 7 to 10 days after birth, followed by weekly tests within the first 4 weeks, and retested at 36 weeks of corrected gestational age or before discharge. The VLBWI were assigned to the CH group or non-CH group. Chi-square test, Fisher exact probability method, Wilcoxon rank sum test, univariate and multivariate Logistic regression were used to analyze the relationship between CH and poor prognosis during hospitalization in VLBWI. Results:A total of 5 818 eligible VLBWI were enrolled, with 2 982 (51.3%) males and the gestational age of 30 (29, 31) weeks. The incidence of CH was 5.5% (319 VLBWI). Among the CH group, only 121 VLBWI (37.9%) were diagnosed at the first screening. Univariate Logistic regression analysis showed that CH was associated with increased incidence of extrauterine growth retardation (EUGR) ( OR=1.31(1.04-1.64), P<0.05) and retinopathy of prematurity (ROP) of stage Ⅲ and above ( OR=1.74(1.11-2.75), P<0.05). However, multivariate Logistic regression analysis showed no significant correlation between CH and EUGR, moderate to severe bronchopulmonary dysplasia, grade Ⅲ to Ⅳ intraventricular hemorrhage, neonatal necrotizing enterocolitis in stage Ⅱ or above, and ROP in stage Ⅲ or above ( OR=1.04 (0.81-1.33), 0.79 (0.54-1.15), 1.15 (0.58-2.26), 1.43 (0.81-2.53), 1.12 (0.70-1.80), all P>0.05). Conclusion:There is no significant correlation between CH and in-hospital adverse outcomes, possibly due to timely diagnosis and active replacement therapy.

Objective To explore the safty and feasibility of transrectal ultrasound guided transperineal seminal vesicle biopsy in the evaluation of clinical staging of prostate cancer.Methods Retrospectively study 57 suspected prostate cancer patients with seminal abnormality during 2010.7-2015.1,age ranged from 50 to 78 years,average 65 ±7 years,serum total prostate specific antigen (tPSA) 3.2-131.1 μg/L, average (23.7 ± 11.3) μg/L.Twenty-two cases had palpable prostate nodules through rectal examination.All the 57 patients underwent ultrasound and template guided transperineal prostate and seminal vesicle puncture biopsies.Results Forty-four cases out of 57 found prostate cancer cells in biopsies, and 32 cases had seminal vesicle invasion (positive group) while the other 12 were negative.Twenty cases had been performed prostatectomy in the positive group and their post-operative pathological examination all showed prostate cancer with seminal vesicle invasion.Eleven cases in the negative group had been performed prostatectomy ,and 2 cases showed seminal vesicle invasion.The clinical stages of all cases in the positive group were considered as T3b both pre-operatively and post-operatively.In the negative group however, 11 cases were considered as T2 stage pre-operatively,while 2 cases were increased to T3b stage post-operatively.The sensitivity of puncturing seminal vesicle was 91％ (20/22) ,specificity was 100.0％ (9/9).Positive predictive value was 100.0％ (20/20),while negative predictive value was 82％ (9/11).All the 57 cases did not present fever after puncture biopsies, while 23 cases presented hematuria (40％) ,20 cases presented hemospermia (35％) and 1 case presented urinary retention (2％).Conclusions Transrectal ultrasound-guided transperinealseminal vesicle puncture is safe and reliable, it helps to improve the accuracy of pre-operative staging.

Objective To summarize the complication of clinical characteristics, factors causing misdiagnosis, and diagnosis of large intestine tuberculosis (LIT). Methods The data of twenty cases of LIT misdiagnosed preoperatively in our hospital were analyzed retrospectively. The difficulties of LIT diagnosis and management were studied and analyzed. Results Abdominal pain, mass, and the alteration of stool habit were the most common symptoms. LIT was most commonly confused with malignant tumor, Crohn's disease, and periappendicular abscess. Ten of 17 patients, who were performed exploratory laparotomy, were misdiagnosed as tumor or Crohn's disease. Most of our cases were not definitively diagnosed until the histopathologic examination after surgery. Three cases were diagnosed by other methods. Gastrointestinal X ray series, colonoscopy, endoscopic fine needle aspiration cytology(FNAC) and laparoscopic exploration may improve the diagnosis. Exploratory laparotomy with biopsy was the final procedure for diagnosis. Final diagnosis mainly depends on histology. Conclusions LIT lacks special clinical manifestations and has a high misdiagnosis rate. But if correct diagnosis is established, most patients can be cured and unnecessary exploratory laparotomy can be avoided. Six months antituberculosis treatment is effective for LIT whether the lesion is excised or not.