Objective To evaluate the efficacy of vitamin AD combined with step-up therapy in children with asthma and its impact on humoral immunity,pulmonary function and recurrence.Meth-ods A total of 94 children with asthma were enrolled,and randomly divided into study group(n=47)and control group(n=47)using random number table method.The control group adopted the step-up treatment plan on the basis of conventional treatment,while the study group was treated with vitamin AD on the basis of the control group.The clinical efficacy,pulmonary function indicators[forced vital capacity(FVC),forced expiratory volume in one second(FEV,),FEV1/FVC and peak expiratory flow rate(PEF)]and humoral immunefunction[immunoglobulin G(IgG),immuno-globulin A(IgA),immunoglobulin M(IgM)and immunoglobulin E(IgE)]after treatment were compared between the two groups.The recurrence rateand safety of the two groups after treatment were compared.Results The total effective rate of clinical efficacy in the study group was 93.62％,which was significantly higher than 78.72％in the control group(P＜0.05).After treatment,FVC,FEV1,FEV1/FVC and PEF levels were significantly higher in both groups compared to pre-treatment values,and the study group showed significantly better improvement than the control group(P＜0.05).After treatment,serum IgG and IgA levels in the study group were significantly higher than those in the control group,whereas IgM and IgE levels were significantly lower(P＜0.05).At 12 months after treatment,the recurrence rate in the study group was 4.26％(2/47),which was sig-nificantly lower than 19.15％(9/47)in the control group(P＜0.05).There was no statistically significant difference in the incidence of adverse reactions between the two groups(P＞0.05).Conclusion Vitamin AD combined with step-up therapy effectively alleviates clinical symptoms,modulates humoral immune balance,improves pulmonary function,and reduces recurrence rates in children with asthma,and has high safety.

Objective:To study the effect of Shenqi Fuzheng injection combined with interferon-α (IFN-α) on the expression of STAT1 gene in hepatocellular carcinoma cells,and to elucidate its mechanism of IFN-α synergistic effect.Methords:The effect of IFN-α injection alone or combined with Shenqi Fuzheng injection on the proliferation of MHCC97-L cells was detected by MTT assay.IFN-α was detected by Real-time PCR and Western-blot respectively.The expression of STAT1 mRNA and protein in the experimental group,the negative control group (NaCl) and the blank control group were determined,and the effect of Shenqi Fuzheng injection on the transcription and expression of STAT1 was determined.The expression of STAT1 in lentiviral vector MTT assay was used to detect the effect of IFN-α alone or in combination with Shenqi Fuzheng injection on the STAT1 gene silencing cells.The expression of STAT1 was detected by RT-PCR and Western-Blot in MHCC97L cells.Strain,on cell proliferation.Results:Compared with IFN-α alone,Shenqi Fuzheng injection combined with IFN-α enhanced the inhibitory effect of IFN-α on MHCC97-L (P＜0.01) and up-regulated the expression of STAT1mRNA and protein (P＜0.05).Successfully constructed the STAT1 gene silent in the MHCC97-L cell line.There was no significant difference in the inhibition rate of MHCC97-L between Shenqi Fuzheng injection and IFN-α (P＞0.05).Conclusion Shenqi Fuzheng Injection can up-regulate the expression of STAT1 in human hepatocellular carcinoma cell line MHCC97-L,so as to increase the effect of IFN-α.