Objective To analyze and evaluate the early warning efficacy of D-dimer/albumin ratio(DAR)combined with interleukin-6(IL-6)and fibrin degradation products(FDP)in the postoperative treatment of acute Stanford type A aortic dissection(ATAAD).Methods A retrospective cohort study was conducted on 284 ATAAD patients who underwent the Sun's procedure at our hospital from July 2024 to March 2025.Patients were divided into a non-adverse outcome group(n=196)and an adverse outcome group(n=88)based on the occurrence of postop-erative complications within 30 days,including acute renal failure requiring dialysis,secondary thoracotomy for hemostasis,severe neurological complications,multiple organ failure,or all-cause mortality.Preoperative baseline data,perioperative parameters,and laboratory indicators were collected via the electronic medical record system.The Mann-Whitney U test was used to compare the differences between groups for continuous variables that did not conform to the normal distribution,and Chi-square test or Fisher's exact test was selected for statistical difference analysis according to the frequency distribution characteristics of categorical variables.On the basis of univariate analysis,multivariate logistic regression analysis was used to screen independent risk factors.Results Statistically significant differences were observed between the non-adverse and adverse outcome group in age,cardiopulmonary bypass time,lactate dehydrogenase(LDH),IL-6,D-dimer(D-D),FDP,and DAR levels(P<0.05).Multivariate analysis revealed that DAR,IL-6,D-D,FDP,and prolonged cardiopulmonary bypass time were independent risk factors for adverse postoperative outcomes(P<0.05).Combined detection analysis demonstrated that the combination of DAR,IL-6,FDP,and cardiopulmonary bypass time yielded the highest predictive efficacy,with an area under the ROC curve of 0.886(95％CI:0.846～0.927).Conclusion The combination of DAR,IL-6,FDP,and cardio-pulmonary bypass time effectively predicts adverse postoperative outcomes in ATAAD patients.This biomarker panel may serve as a robust predictive tool for postoperative risk stratification.

Objective To analyze and evaluate the early warning efficacy of D-dimer/albumin ratio(DAR)combined with interleukin-6(IL-6)and fibrin degradation products(FDP)in the postoperative treatment of acute Stanford type A aortic dissection(ATAAD).Methods A retrospective cohort study was conducted on 284 ATAAD patients who underwent the Sun's procedure at our hospital from July 2024 to March 2025.Patients were divided into a non-adverse outcome group(n=196)and an adverse outcome group(n=88)based on the occurrence of postop-erative complications within 30 days,including acute renal failure requiring dialysis,secondary thoracotomy for hemostasis,severe neurological complications,multiple organ failure,or all-cause mortality.Preoperative baseline data,perioperative parameters,and laboratory indicators were collected via the electronic medical record system.The Mann-Whitney U test was used to compare the differences between groups for continuous variables that did not conform to the normal distribution,and Chi-square test or Fisher's exact test was selected for statistical difference analysis according to the frequency distribution characteristics of categorical variables.On the basis of univariate analysis,multivariate logistic regression analysis was used to screen independent risk factors.Results Statistically significant differences were observed between the non-adverse and adverse outcome group in age,cardiopulmonary bypass time,lactate dehydrogenase(LDH),IL-6,D-dimer(D-D),FDP,and DAR levels(P<0.05).Multivariate analysis revealed that DAR,IL-6,D-D,FDP,and prolonged cardiopulmonary bypass time were independent risk factors for adverse postoperative outcomes(P<0.05).Combined detection analysis demonstrated that the combination of DAR,IL-6,FDP,and cardiopulmonary bypass time yielded the highest predictive efficacy,with an area under the ROC curve of 0.886(95％CI:0.846～0.927).Conclusion The combination of DAR,IL-6,FDP,and cardio-pulmonary bypass time effectively predicts adverse postoperative outcomes in ATAAD patients.This biomarker panel may serve as a robust predictive tool for postoperative risk stratification.

Objective Through the combined detection method,we tried to find more sensitive biomarkers for the diagnosis of acute TBI,which might provide the exact diagnosis,treatment and monitoring indicators for the clinic.Methods A total of 156 patients with acute TBI admitted to the Emergency Traumatic Center of Yan'an Hospital of Kunming from October 2022 to June 2023 were collected and included the normal and fracture control groups.Peripheral blood samples of the enrolled patients were collected and monitored,S100β and IL-6 were tested and clinical data such as hs-CRP was collected immediately upon admission,then statistical analysisanalysis was conducted.Results Peripheral blood in patients with acute TBI,The levels of S100β,IL-6 and hs-CRP significantly increased,and the area under the ROC curve for diagnosing acute TBI was 0.944,0.915,and 0.897.The combined detection of the three indicators showed an area under the ROC curve of up to 0.975.Person correlation analysis found a positive correlation between the three indicators,especially S100β with IL-6,The correlation coefficient between them is 0.715.Binary logistic regression analysis found that S100β,IL-6 and hs-CRP are independent risk factors for acute TBI.Conclusions The combined detection of S100β,IL-6 and hs-CRP can effectively improve the sensitivity of acute TBI diagnosis.The mutual promotion of S100β and IL-6 may aggravate the secondary craniocerebral injury caused by inflammatory mechanism,and early targeted treatment may improve the prognosis.

Objective: To investigate the clinical features of Hashimoto′s thyroiditis(HT) with elevated serum IgG4 levels and to guide the clinical practice. Methods: Serum IgG4 concentrations were detected in patients with HT by nephelometric immunoassay. These patients were classified into two groups according to the IgG4 levels: positive group(IgG4≥1.35 g/L), and negative group(IgG4<1.35 g/L). The clinical characteristics of these two groups, including age, gender, medical history, serological features, and ultrasound were compared. Results: Nine out of 111 patients with HT were positive with IgG4(8.11%), all of them were women. There was no significant difference in gender, age, BMI, dose of levothyroxine administration, FT₃, FT₄, and TSH levels between the two groups. The volume of thyroid in positive group was larger than that in negative group [57.81(38.36, 74.93) ml vs 25.07(18.48, 42.14) ml, P=0.015], and the level of thyroglobulin antibodies(TgAb; P=0.011) and thyroid peroxidase antibodies(TPOAb; P=0.025) in positive group were also significantly higher than those in negative group. Moreover, the positive group had a slightly higher risk of PTC than that of negative group(11.1% vs 2.94%, P=0.290), though the difference was not significant. Correlation analysis showed that IgG4 was positively associated with TgAb, TPOAb, and thyroid volume. Conclusions The levels of TgAb and TPOAb are higher, the volume of thyroid gland is greater, and the risk of PTC with lymph node metastasis is more frequent in HT patients with elevated serum IgG4. For HT patient with elevated serum IgG4, their thyroid function and morphology should be more closely monitored.

Objective:To investigate the clinical outcomes of in vitro fertilization-embryo transfer (IVF-ET) between donor and recipient. Methods:Pregnancy outcomes of 31 donors of infertility patients of ovarian hyperresponsiveness and 30 recipients receiving by IVF-ET from January 2011 to December 2016 in the Reproductive Center of Henan Provincial People's Hospital were retrospectively analyzed.Results:The implantation rate of frozen-thawed embryo transfer (FET) cycle (52.05% vs. 58.70%), the clinical pregnancy rate (69.23% vs.73.91%), the live birth rate per transplant cycle (61.54% vs.65.22%), the cumulative pregnancy rate per oocyte pick-up cycle (87.10% vs. 100.00%), the twin rate (40.74% vs. 48.39%), and the live birth rate (77.42% vs. 96.77%) were slightly higher in donor group than in recipient group, while there were no significant differences ( P>0.05). There were no significant differences in implantation rate (58.70% vs. 40.74%), clinical pregnancy rate (76.00% vs. 57.14%), early abortion rate (5.26% vs. 12.50%) and live birth rate per transplant cycle (72.00% vs. 42.86%) between group of <38 years old and group of ≥38 years old ( P>0.05). There were significant differences in cumulative pregnancy rate (100.00% vs. 66.67%) and live birth rate per oocyte pick-up cycle (94.74% vs. 50.00%) ( P=0.037, P=0.007). Conclusion:Oocyte donation IVF-ET is an effective method to assist infertility patients such as premature ovarian failure, perimenopausal period, chromosomal abnormalities, and repeated failure of IVF-ET. The success rate of pregnancy in the recipients of oocyte is similar to that in the donors. All different age groups of recipients can obtain good pregnancy outcomes, and the young recipients have a higher live birth rate.

Objective:To investigate the clinical outcomes of in vitro fertilization-embryo transfer (IVF-ET) between donor and recipient. Methods:Pregnancy outcomes of 31 donors of infertility patients of ovarian hyperresponsiveness and 30 recipients receiving by IVF-ET from January 2011 to December 2016 in the Reproductive Center of Henan Provincial People's Hospital were retrospectively analyzed.Results:The implantation rate of frozen-thawed embryo transfer (FET) cycle (52.05% vs. 58.70%), the clinical pregnancy rate (69.23% vs.73.91%), the live birth rate per transplant cycle (61.54% vs.65.22%), the cumulative pregnancy rate per oocyte pick-up cycle (87.10% vs. 100.00%), the twin rate (40.74% vs. 48.39%), and the live birth rate (77.42% vs. 96.77%) were slightly higher in donor group than in recipient group, while there were no significant differences ( P>0.05). There were no significant differences in implantation rate (58.70% vs. 40.74%), clinical pregnancy rate (76.00% vs. 57.14%), early abortion rate (5.26% vs. 12.50%) and live birth rate per transplant cycle (72.00% vs. 42.86%) between group of <38 years old and group of ≥38 years old ( P>0.05). There were significant differences in cumulative pregnancy rate (100.00% vs. 66.67%) and live birth rate per oocyte pick-up cycle (94.74% vs. 50.00%) ( P=0.037, P=0.007). Conclusion:Oocyte donation IVF-ET is an effective method to assist infertility patients such as premature ovarian failure, perimenopausal period, chromosomal abnormalities, and repeated failure of IVF-ET. The success rate of pregnancy in the recipients of oocyte is similar to that in the donors. All different age groups of recipients can obtain good pregnancy outcomes, and the young recipients have a higher live birth rate.

Objective To investigate the effect of short-term very low calorie diet on the thyroid function in patients with metabolic syndrome.Method Fifty-nine patients [(23 males and 36 females,average age (38.17 ± 9.61) years] with metabolic syndrome were received very low calorie diet for 9 days in our hospital.Anthropometric and biochemical parameters were determined before and after very low calorie diet.The level of serum FT3,FT4,TSH was measured by chemiluminescent assay,and the level of serum reverse triiodothyronine (rT3) was measured by radioimmunoassay in 31 cases.Homeostasis model assessment for insulin resistance (HOMA-IR) was employed to evaluate the severity of insulin sensitivity.Results After short-term very low calorie diet,the average body weight decreased from (76.30± 19.61)kg to (71.97 ± 18.58)kg (P＜0.01);waist circumference reduced by 6.98 cm (P＜ 0.01).The blood pressure,total cholesterol,triglycerides,low density lipoprotein-cholesterol,fasting blood glucose,fasting insulin,and HOMA-IR were all reduced significantly after very low calorie diet.The serum FT3 level decreased from (5.09 ± 0.58) pmol/L to (4.52 ± 0.58) pmol/L (P＜0.01).The changes of FT4,TSH,and rT3 were without difference.The basal FT3 levels were positively correlated with basal body weight,body mass index,waist circumference (r =0.335,0.297,and 0.262 respectively,all P＜ 0.05),and negatively correlated with HbAIC,fasting blood glucose (r=-0.293,-0.268,both P＜0.05).The basal FT4 level was negatively correlated with basal fasting insulin,HOMA-IR (r =-0.284,-0.330,both P＜0.05),while the basal TSH level was positively correlated with fasting insulin and HOMA-IR (r=0.381,0.281,both P＜0.05).The basal body weight was positively correlated with waist circumference,fasting insulin,HOMA-IR,while negatively correlated with high density lipoproteincholesterol (HDL-C;r =0.899,0.382,0.305,-0.333,all P＜0.05).After very low calorie diet,there were no more significant correlations between FT3 changes and baseline body weights,waist circumferences,and fasting blood glucose levels.The variation amplitude of body weight was positively correlated with baseline weight,waist circumference,fasting insulin,HOMA-IR,and FT3 levels (r =0.564,0.494,0.307,0.245,0.384,all P＜ 0.05).Conclusions Short-term very low calorie diet decreases the serum FT3 level and improves metabolic markers in patients with metabolic syndrome.The serum FT3 level of patients with metabolic syndrome was positively associated with body weight,whereas negatively associated with HbAIC and fasting blood glucose.Moreover,FT4 level had negative correlation with HOMA-IR,but TSH level had positive correlation with HOMA-IR.Those who had higher body weights,FT3 and HOMA-IR levels,may have more profound decrease of body weight after very low calorie diet.

Objective To study the expression of tumor necrosis factor -α (TNF-a) and correlation with TNF-a in breast cancer. Methods We used SABC method to detect the expression of TNF-a in 112 cases of primary breast cancer, and analysis the relationship between TNF-α with histologic grades and ER. Results The positive expression rate of TNF-α in breast cancer was higher than those of normal tissues and lobular hyperplasia of the breast (X2 =8.573, P =0.014). There was no significant correlation between histologic grades and TNF-α (X2 =1.304, P =0.521). TNF-a had a positive correlation with ER in breast cancer (X2=11.949, P =0.001). Conclusion The positive expression rate of TNF-α in breast cancer was higher than those of normal tissues and lobular hyperplasia of the breast.TNF-α had a positive correlation with ER in breast cancer, but the exact mechanism is unclear.

Objective To determine the expression of Human Epidermal Growth Factor Receptor 2 (HER-2) in breast cancer and its correlation with Tumor Necrosis Factor-α (TNF-α). Methods We used SABC method to detect the expression of HER-2 and TNF-α in 112 cases of primary breast cancer, and analyzed the association of HER-2 expression with clinicopathological features and TNF-α expression. Results The positive expression rate of HER-2 in breast cancer was 35.71% (40/112), of which, the expression rate was 35.05% (34/97) in 97 invasive tubic breast cancer, and 33.33% (2/6) in invasive lobular breast cancer,25.00% (1/4) in mucinous carcinoma, and 50. 00% (2/4) in intraductal carcinoma. There was no significant correlation between HER-2 and any clinicopathological features of primary breast cancer ( P ＞ 0. 05 ). The expression of HER-2 in breast cancer had no signiciant association with TNM stage, age, age of menarche,menopause, gravidity, parity and family history of cancer ( Ps ＞ 0. 05 ), The positive rate of TNF-α was 23.61% (17/72) when HER-2 was negative, whereas the positive rate of TNF-α was 52. 50% (21/40) when HER-2 was positive. The expression of HER-2 was signiciantly positively correlated with the expression of TNF-α (r = 0. 881, P ＜ 0. 05 ). Conclusion HER-2 expression had a positive correlation with TNF-α in breast cancer,but the specific mechanism is still unclear.

OBJECTIVE: To establish Caco-2 (a human colon adenocarcinoma cell line) cell monolayer model and the standard operation procedure for studying and assessing intestinal absorption of chemical components of traditional Chinese medicine. METHODS: Caco-2 cell monolayer model was established and evaluated by morphology feature using scanning electron microscope, inverted microscope and transepithelial electrical resistance (TEER) assay. Additionally, the model was further tested for the activity of alkaline phosphatase and the apparent permeability (Papp) of standard compounds, i.e. propranolol and atenolol, which were the control substances for high and poor transcellular transport marker, respectively. RESULTS: The integrality of cell monolayer, cell differentiation (reflected by expression of alkaline phosphatase and cell monolayer morphology), and the Papp value of standard compounds in the established Caco-2 cell model were satisfactory. All parameters tested were in good agreement with those reported in the literature. CONCLUSION: The established Caco-2 cell model can be used to study the intestinal absorption of orally administrated chemical components of traditional Chinese medicine and their absorption mechanism.