Objective To investigate the effect of long non-coding RNA(LncRNA)NNT-AS1 on proliferation,migration and invasion of oral squamous cell carcinoma(OSCC)cells by regulating microRNA(miR)-199a-5p.Methods CAL-27 cells were assigned into the control group,the sh-NC group,the sh-NNT-AS1 group,the sh-NNT-AS1+miR-In-NC group and the sh-NNT-AS1+miR-199a-5p-In group.The proliferation,colony formation,migration and invasion abilities of CAL-27 cells were detected by EdU method,clone formation,scratch healing and Transwell experiments.The expressions of LncRNA NNT-AS1 and miR-199a-5p in human keratinocyte cell line HaCaT,human OSCC cell line CAL-27 and each group of cells were detected by qRT-PCR experiment.Western blot experiments were performed to detect the expressions of epithelial-mesenchymal transition(EMT)-related proteins[E-cadherin,N-cadherin,Vimentin,and epidermal growth factor receptor(EGFR)].The targeting relationship between LncRNA NNT-AS1 and miR-199a-5p was verified by dual luciferase reporter gene assay.Results Compared with HaCaT cells,the level of LncRNA NNT-AS1 was elevated in CAL-27 cells,while the level of miR-199a-5p was decreased(P＜0.05).Compared with the control group and the sh-NC group,the EdU positive rate,colony formation rate,scratch healing rate,cell invasion number,expression levels of LncRNA NNT-AS1,N-cadherin,Vimentin and EGFR proteins were decreased in the sh-NNT-AS1 group,while the expression levels of miR-199a-5p and E-cadherin protein were increased(P＜0.05).Compared with the sh-NNT-AS1 group and the sh-NNT-AS1+miR-In-NC group,the EdU positive rate,colony formation rate,scratch healing rate,cell invasion number,N-cadherin,Vimentin and EGFR protein expression levels were increased in the sh-NNT-AS1+miR-199a-5p-In group,while the expression levels of miR-199a-5p and E-cadherin protein were decreased(P＜0.05).Compared with the miR-NC group,the relative luciferase activity of NNT-AS1-WT cells transfected with miR-199a-5p mimic was decreased(P＜0.05).Conclusion LncRNA NNT-AS1 is highly expressed in OSCC.It can promote the proliferation,migration,invasion and EMT process of OSCC cells by negatively regulating miR-199a-5p.

Objective To investigate the effect of long non-coding RNA(LncRNA)NNT-AS1 on proliferation,migration and invasion of oral squamous cell carcinoma(OSCC)cells by regulating microRNA(miR)-199a-5p.Methods CAL-27 cells were assigned into the control group,the sh-NC group,the sh-NNT-AS1 group,the sh-NNT-AS1+miR-In-NC group and the sh-NNT-AS1+miR-199a-5p-In group.The proliferation,colony formation,migration and invasion abilities of CAL-27 cells were detected by EdU method,clone formation,scratch healing and Transwell experiments.The expressions of LncRNA NNT-AS1 and miR-199a-5p in human keratinocyte cell line HaCaT,human OSCC cell line CAL-27 and each group of cells were detected by qRT-PCR experiment.Western blot experiments were performed to detect the expressions of epithelial-mesenchymal transition(EMT)-related proteins[E-cadherin,N-cadherin,Vimentin,and epidermal growth factor receptor(EGFR)].The targeting relationship between LncRNA NNT-AS1 and miR-199a-5p was verified by dual luciferase reporter gene assay.Results Compared with HaCaT cells,the level of LncRNA NNT-AS1 was elevated in CAL-27 cells,while the level of miR-199a-5p was decreased(P＜0.05).Compared with the control group and the sh-NC group,the EdU positive rate,colony formation rate,scratch healing rate,cell invasion number,expression levels of LncRNA NNT-AS1,N-cadherin,Vimentin and EGFR proteins were decreased in the sh-NNT-AS1 group,while the expression levels of miR-199a-5p and E-cadherin protein were increased(P＜0.05).Compared with the sh-NNT-AS1 group and the sh-NNT-AS1+miR-In-NC group,the EdU positive rate,colony formation rate,scratch healing rate,cell invasion number,N-cadherin,Vimentin and EGFR protein expression levels were increased in the sh-NNT-AS1+miR-199a-5p-In group,while the expression levels of miR-199a-5p and E-cadherin protein were decreased(P＜0.05).Compared with the miR-NC group,the relative luciferase activity of NNT-AS1-WT cells transfected with miR-199a-5p mimic was decreased(P＜0.05).Conclusion LncRNA NNT-AS1 is highly expressed in OSCC.It can promote the proliferation,migration,invasion and EMT process of OSCC cells by negatively regulating miR-199a-5p.

With the change of COVID-19 prevention and control strategy in China, the number of COVID-19 cases has increased significantly recently, which has also brought new challenges to the perioperative risk control of thoracic surgery. This paper puts forward several suggestions, aiming to standardize the preoperative screening and evaluation during the COVID-19 period, strictly grasp the indications and timing of surgery, optimize the medical management process, individualize surgical decision-making, and minimize the risk of COVID-19 infection to surgery.

With the change of coronavirus disease 2019 (COVID-19) prevention and control strategy in China, the number of COVID-19 cases has increased significantly recently, which has also brought new challenges to the perioperative risk control of thoracic surgery. This paper puts forward several suggestions, aiming to standardize the preoperative screening and evaluation during the COVID-19 period, strictly grasp the indications and timing of surgery, optimize the medical management process, individualize surgical decision-making, and minimize the risk of COVID-19 infection to surgery.

The role of insulin pump in the treatment of diabetes is becoming more and more significant. Based on the systematic literature review, collation and analysis, this article introduces the management of insulin pump outside the hospital from the development trend of insulin pump, application and common problems. In order to enhance the therapeutic satisfaction of patients and improve their quality of life, our aims are to put forward the strategy of standardized management and provide a reference for establishing and improving the standardized management system of insulin pump therapy outside hospital.

BACKGROUND:Open fracture of lower limb with severe soft tissue and bone defects also accompanies anterior tibial soft tissue defects and exposure of sclerotin and steel plate, which can be crucial y treated with strong fixation and use of skin flap to block the wound.
OBJECTIVE:To explore the clinical efficacy of a large area of soft tissue defects in the anterior tibia using vacuum sealing drainage combined with groin free flap.
METHODS:A total of 24 patients with a large area of soft tissue defects in the anterior tibia were included in this study and then divided into two groups, with 12 cases in each group. In vacuum sealing drainage group, the scope of soft tissue defects was ranged from 10 cm×15 cm to 15 cm×20 cm. After the debridement, the fracture was fixed with external fixation scaffold and the wound was covered with the vacuum sealing drainage dressing. The blood clot was rinsed with normal saline via T-tube, and 7-10 days later the vacuum sealing drainage was given. According to the growth of granulation tissue, the wound was secondarily sutured, fol owed by groin free skin flap of superficial iliac circumflex artery with medial knee arteriovenous anastomosis transplantation. In the non-vacuum sealing drainage group, the wound size was ranged from 10 cm×5 cm to 30 cm×20 cm, the period from injury to admission was 1-24 hours. They were given conventional debridement and secondary fixation or skin flap transplantation.
RESULTS AND CONCLUSION:The length of preoperative hospital stay and the skin flap are in vacuum sealing drainage group were significantly better than those in non-vacuum sealing drainage group (P<0.05). There was no significant difference in the length of postoperative stay and total length of hospital stay between the two groups (P>0.05). The wound infection rate was 0 in vacuum sealing drainage group and 75%in non-vacuum sealing drainage group at 8-14 days after treatment. The wound and donor area incision were healed at I stage, the skin grafts survived. Al the involved patients in two groups were fol owed up, for 6-36 months. During the fol ow-up process, the fracture healing time in non-vacuum sealing drainage group was significantly longer than that in vacuum sealing drainage group. The skin flap in two groups was similar to surrounding skin in color and texture, the flap exhibited no vessels, no ulceration, and no clumsy. The vacuum sealing drainage combined with groin free flap can timely control a large area of soft tissue defects post-trauma, improve wound blood supply, shorten preoperative preparation time, early close the wound, significantly promote the healing of wound and fracture. The skin flap is soft, flexible, wel-looking, and active functional, it significantly shortens the course of treatment and maximizes the recovery of limb function.