Objective To investigate the dynamic changes of serum regulatory T cell(Treg cell)-related factors in patients with diabetic peripheral neuropathy(DPN)and their relationships with pain mediators.Methods A total of 140 patients with DPN were enrolled and divided into painful DPN group(36 patients)and painless DPN group(104 patients)based on the presence of neuropathic pain.The serum levels of Treg cell-related factors[interleukin-10(IL-10),transforming growth fac-tor-β1(TGF-β1),interleukin-35(IL-35)]and pain mediators[prostaglandin E2(PGE2),hista-mine(His),and 5-hydroxytryptamine(5-HT)]between the two groups were compared.Pearson cor-relation analysis was used to explore the correlations of the serum levels of IL-10,TGF-β1,and IL-35 with the levels of PGE2,His,and 5-HT in patients with DPN.Multiple linear regression analysis was conducted to investigate the influencing factors of pain intensity in patients with DPN.Results The serum levels of TGF-β1,PGE2,His,and 5-HT were higher,while the levels of IL-10 and IL-35 were lower in the painful DPN group than those in the painless DPN group(P＜0.05).Pearson correlation a-nalysis showed that IL-10 was negatively correlated with PGE2,His,and 5-HT(r=-0.781,-0.794,-0.714,P＜0.05);TGF-β1 was positively correlated with PGE2,His,and 5-HT(r=0.605,0.590,0.515,P＜0.05);IL-35 was negatively correlated with PGE2,His,and 5-HT(r=-0.727,-0.847,-0.727,P＜0.05).Multiple linear regression analysis revealed that IL-10,TGF-β1,and IL-35 were closely related to the pain intensity in patients with DPN and were influencing factors of painin-tensity(P＜0.05).Conclusion The serum levels of IL-10 and IL-35 in patients with DPN are negatively correlated with the levels of PGE2,His,and 5-HT,while the level of TGF-β1 is positive-ly correlated with these pain mediators.Furthermore,IL-10,TGF-β1,and IL-35 are closely related to the degree of neuropathic pain in patients with DPN.

Objective:To investigate the levels of chemokine 2 (CCL2), chemokine (C-X-C motif) ligand 1 (CXCL1), chemokine (C-X3-C motif) ligand 1 (CX3CL1), and chemokine (C-X-C motif) ligand 10 (CXCL10) in the cerebrospinal fluid of patients with acute and chronic herpes zoster (HZ), and their correlation with the severity of neuropathic pain.Methods:A total of 60 patients with acute herpes zoster and postherpetic neuralgia (PHN) treated at the First Affiliated Hospital of Zhengzhou University from November 2022 to November 2023 were selected for a case-control study. These patients were divided into a HZ group ( n = 25) and a PHN group ( n = 35). The pressure pain threshold in the affected area was measured on the day of admission for all patients. The Visual Analogue Scale scores were evaluated in both groups. After lumbar puncture, 2 mL of cerebrospinal fluid was collected, and the levels of related chemokines in the cerebrospinal fluid were detected using the enzyme-linked immunosorbent assay. Results:In the HZ group, the levels of chemokines CXCL1 and CXCL10 were (24.84 ± 6.97) ng/L and (107.46 ± 28.29) μg/L, respectively, while in the PHN group, they were (20.51 ± 3.16) ng/L and (132.98 ± 30.92) μg/L. The differences in the levels of chemokines CXCL1 and CXCL10 between the two groups were statistically significant ( t = 2.91, -3.26, both P < 0.05). There were no statistically significant differences in the levels of chemokines CCL2 and CX3CL1 between the two groups ( t = 1.62, -0.23, both P > 0.05). The levels of CCL2, CXCL1, CX3CL1, and CXCL10 in both groups were negatively correlated with the pressure pain threshold (HZ group: r = -0.84, -0.78, -0.93, -0.86; PHN group: r = -0.71, -0.78, -0.94, -0.76, all P < 0.01). Conclusion:Higher levels of chemokines in the cerebrospinal fluid of patients with acute and chronic HZ are associated with more pronounced neuropathic pain. The neuro-immune inflammation involving chemokines may be correlated with the severity of neuropathic pain in acute and chronic HZ.

Objective This study aims to evaluate the changes of Cdk5 expression at the time of 3 hours to 10 days after moderate brain injury by blunt force impact in a rat model,and to demonstrate its forensic significance.Methods To establish a rat model of blunt focal brain contusion,and to observe the changes of Cdk5 expression in brain tissue at different timepoints after brain injury by immunohistochemistry and Western blot.Results A low expression level of Cdk5 was observed in the brain tissue of both normal and sham control groups.The expression of Cdk5 increased after 3 and 6 hours,remarkably increased at 12 hours,and reached the maximal level at 24 hours after focal brain injury.The Cdk5 level gradually decreased 3 days,5 days,7 days,and 10 days and reached the normal level 7 and 10 days after the injury,with no statistical difference (P＞0.05) compared with the normal and sham control groups.Conclusion The expression of Cdk5 increased in the peripheral area of contusion tissue after blunt brain injury in rats,showing single peak change,and dropped to normal level with the time extension.The change of Cdk5 expression may provide a new reference index for the prediction of early brain contusion.

OBJECTIVETo study the dynamic changes of dry material accumulation and platycodin D content in Platycodon grandiflorum in different planting densities.

METHODFive different planting densities M1 (4 cm x 25 cm), M2 (6 cm x 25 cm), M3 (8 cm x 25 cm), M4 (10 cm x 25 cm) and M5 (12 cm x 25 cm) were designed in the plot experiment. The individual and colony biomass accumulation, dry material distribution, root yield and platycodin D content were measured in different stage.

RESULTIn a certain density range the individual biomass in P. grandiflorum obviously declined with increasing density with the exception of biomass M2 > biomass M3. On the contrary, the colony biomass increased with the increasing density. Dry material accumulation in each organ in P. grandiflorum in different planting densities showed significance (P<0.05). The dry material distribution in organs in the different planting densities showed significance (P<0.05), and the dry material distribution in flower and fruit reached the minimal level in M2, in the same planting density the distribution in root reached the maximal; The dry material in stem, flower and fruit obviously declined with the increasing density, while the dry material in leaf increased. The individual root output increased with the increasing density, and it reached the highest in M2. The colony root yield increased with the increasing density. The platycodin D content in P. grandiflorum reached the highest in M2.

CONCLUSIONThe result showed that a suitable planting density is very important to P. grandiflorum dry material accumulation and distribution, root yield, platycodin D content and colony yield.

Plant Structures ; growth & development ; metabolism ; Platycodon ; growth & development ; metabolism ; Saponins ; metabolism ; Seasons ; Triterpenes ; metabolism

0.05).Conclusion:Different polishing and glazing methods have significant effects on the surface roughness of ceramics,while the surface gloss of ceramics is not affected.