Based on the thinking of programmatization and proceduralization， this study integrated traditional Chinese medicine （TCM） classic theories with modern knowledge expression technologies to construct a "formula-symptom" syndrome differentiation thinking model centered on "symptom clustering-main syndrome screening-formula adaptation"， and explored the standardization and intelligentization path of TCM syndrome differentiation and treatment. By establishing the mapping relationship model between formulas and syndromes including quantitative weight analysis of chief， deputy， assistant and envoy medicines， designing the logical hierarchical structure of formula-syndrome decision tree （application of three-level decision tree and fuzzy logic）， and formulating the procedural design of four diagnostic methods （structured collection， correlation model， and dynamic correction mechanism）， the standardization and visualization of the syndrome differentiation process are realized. This model can be transformed into the core data set for artificial intelligence training. Through ternary knowledge graph and machine learning algorithms， it can improve the repeatability of syndrome differentiation and the efficiency of diagnosis and treatment， and implement the strategy of "group model + individual modification" to balance the conflict between quantification and individualization. The core value of this model lies in promoting the objectification and precision development of TCM syndrome differentiation and treatment through the integration of traditional syndrome differentiation thinking and modern system science.

Objective: To analyze the nucleic acid load of human parvovirus B19 in major commercially available blood products in China, including human albumin, human intravenous immunoglobulin, human rabies immunoglobulin and various coagulation factor products, aiming to provide evidence for improving blood product manufacturing processes and quality control of source plasma. Methods: A total of 98 batches of coagulation factor products were tested for human parvovirus B19 nucleic acid using real-time fluorescent quantitative PCR, including 42 batches of human prothrombin complex, 35 batches of human coagulation factor Ⅷ, and 21 batches of human fibrinogen. Additionally, 6 batches of human albumin, 6 batches of human intravenous immunoglobulin, and 38 batches of human rabies immunoglobulin were tested for human parvovirus B19 nucleic acid. Results: Human parvovirus B19 nucleic acid were undetectable in human albumin, human intravenous immunoglobulin and human rabies immunoglobulin. Among the 98 batches of coagulation factor products tested for human parvovirus B19 nucleic acid, B19 nucleic acid reactivity rate was 69.0% (29/42) for human prothrombin complex batches, but nucleic acid concentration were all significantly lower than 10 IU/mL. The reactivity rate of B19 nucleic acid in 35 batches of human coagulation factor Ⅷ was 48.6% (17/35), with nucleic acid concentration all below 10 IU/mL. The reactivity rate of B19 nucleic acid in 21 batches of human fibrinogen was 61.9% (13/21), with nucleic acid concentration all below 10 IU/mL. Conclusion: No human parvovirus B19 has been detected in human albumin, human intravenous immunoglobulin, or human rabies immunoglobulin. Human parvovirus B19 nucleic acid may exist in commercially available coagulation factor products, highlighting the need for enhanced screening of human parvovirus B19 nucleic acid in these products. It is also recommended that B19 viral nucleic acid testing be conducted on source plasma, particularly for coagulation factor products.

Amide proton transfer-weighted (APTw) imaging is a kind of new magnetic resonance molecular imaging that rapidly developed in recent years. APTw imaging reflects the protein concentration and acid-base changes in tissues by detecting amide protons in free proteins and peptides in tissues. APTw imaging is a complementary technique to the existing magnetic resonance techniques, which can provide new molecular biological information for central nervous system diseases. In this article, the basic principle of APTw imaging and its application in common central nervous system diseases such as brain tumor, ischemic stroke, and neurodegenerative diseases are reviewed to provide new methods for promoting the transformation of APTw imaging from the research stage to routine clinical application, and facilitating the precise diagnosis and treatment of central nervous system diseases.

Objective:To revise the quality standards of purified siliceous earth in the Chinese Pharmacopoeia 2020 Vol Ⅳ.Methods:Referring to USP2024 and GB 14936-2012 food additive purified siliceous earth and related ref-erences,the current quality standards for pharmaceutical excipient purified siliceous earth were revised,including description,dissolved substances in water,dissolved substances in acid,and content determination.The soluble a-luminum content was also investigated.Results:Revised the description of characteristic items,methods for deter-mining dissolved substances in water and acid,and limits for content determination;Through the investigation of soluble aluminum before and after filtration of human serum albumin(HSA)samples using purified siliceous earth as a filter aid,it is shown that purified siliceous earth has a certain degree of adsorption effect on aluminum ele-ment.At present,the use of low-temperature ethanol ultrafiltration process does not increase the residual aluminum content in biological products,so an aluminum element determination item is not added to the standard.Conclusion:The comprehensive quality standards for purified siliceous earth will be included in the Chinese Phar-macopoeia 2025 edition,providing strong technical support for the quality and safety supervision of this product.

Objective:To explore the predictive value of MR diffusion kurtosis imaging(DKI)-derived quantitative parameters combined with serum breast cancer susceptibility gene 1(BRCA1)level for postoperative recurrence and metastasis in breast cancer patients.Methods:A total of 73 patients with breast cancer who were treated in our hospital from Mar 2023 to Jan 2024 were selected as the study group,and another 73 patients with benign breast nodules who were treated in the same period were selected as the control group.The quantitative parameters of MR DKI[mean diffusion rate(MD)and diffusion kurtosis(MK)]and serum BRCA1 level were compared between the two groups and among subgroups of the study group with different clinical pathological characteristics,and the correlation was further analyzed.According to the recurrence status within 6 months after surgery,the patients were divided into recurrence/metastasis group(n=24)and non-recurrence/metastasis group(n=49).The MD,MK and serum BRCA1 level were compared between the two groups,and the predictive value was analyzed using the receiver operating characteristic(ROC)curve.Results:MD,MK,and serum BRCA1 level in the study group were higher than those in the control group(P＜0.05).Significant differences in MD,MK,and BRCA1 levels were observed among patients with different differentiation grades,clinical stages,and lymph node metastasis(P＜0.05).MD,MK,and serum BRCA1 level were positively correlated with clinical stage and lymph node metastasis,and negatively correlated with differentiation grades(P＜0.05).The recurrence/metastasis group exhibited higher MD,MK,and BRCA1 levels than the non-recurrence/metastasis group(P＜0.05).The area under the curve of the combined detection of MD,MK,and serum BRCA1 level for the recurrence of breast cancer patients was 0.856,with sensitivity of 91.67%and specificity of 79.59%.Conclusions:The quantitative parameters of MR DKI and serum BRCA1 level are closely related to clinical stages,differentiation degree,lymph node metastasis,and postoperative recurrence and metastasis,demonstrating significant value in early diagnosis,treatment,and prognosis of breast cancer.

Objective To investigate the expression of microRNA-1205(miR-1205)and tripartite motif containing 44(TRIM44)mRNA in colorectal cancer(CRC)tissues,and their correlation with pathological characteristics and prognosis.Methods A total of 140 CRC patients who underwent surgery in the hospital from January 2016 to June 2021 were enrolled as the CRC group,while 140 patients with colorectal adenomas who underwent pathological biopsies during the same period were included as the control group.Real-time flu-orescence quantitative polymerase chain reaction was used to detect the expression of miR-1205 and TRIM44 in CRC and colorectal adenoma tissues.The correlation between miR-1205 and TRIM44 expression and patho-logical parameters was analyzed.Binding sites between miR-1205 and TRIM44 were predicted using an online database,and Pearson correlation was used to analyze the correlation between miR-1205 and TRIM44 expres-sion.Based on miR-1205 and TRIM44 expression in CRC tissues,CRC patients were divided into high-expres-sion and low-expression groups.Kaplan-Meier method was used to plot survival curves of CRC patients in dif-ferent miR-1205 and TRIM44 expression groups.Cox regression analysis was used to investigate the influen-cing factors of mortality in CRC patients.Results The expression level of miR-1205 in the CRC group was lower,while the expression level of TRIM44 was higher compared to the control group(P＜0.05).There was a binding site at the 11583-11590 position in the 3'-untranslated region of TRIM44 and miR-1205.miR-1205 expression was negatively correlated with TRIM44 expression in CRC tissues(P＜0.05).There were statisti-cally significant differences in the expression levels of miR-1205 and TRIM44 among CRC patients with differ-ent differentiation levels,TNM stages,and lymph node metastasis(P＜0.05).The 3-year overall survival rate of the 140 CRC patients was 82.14％(115/140).The 3-year overall survival rate in the high miR-1205 ex-pression group was higher than in the low miR-1205 expression group,while the 3-year overall survival rate was lower in the high TRIM44 expression group compared to the low TRIM44 expression group(P＜0.05).After adjusting for confounding factors,miR-1205≥0.63 was an independent protective factor for mortality in CRC patients(P＜0.05),while TRIM44≥2.84 was an independent risk factor for mortality in CRC patients(P＜0.05).Conclusion miR-1205 is lowly expressed and TRIM44 is highly expressed in CRC tissues,both of which are associated with adverse pathological features and prognosis,and may become prognostic markers for CRC patients.

Objective To investigate the work capability need of offshore workers,and then to construct the evaluation indicator system of work capability for different positions of offshore workers.Methods Literature search,cluster analysis,key events,weight calculation and expert assessment were applied in this study.Results After the indicator screening,weight calculation and expert verification,the work capability evaluation indicator system was established,which contained 5 dimensions and 24 items.The indicators were sequenced according to the weight calculation,and the coefficient of variation of each indicator was less than 20%.Conclusion The construction of the work capability evaluation indicator system for different positions of offshore workers not only improves the content of the offshore job performance evaluation system,but also meets the demand for enhancing offshore operation support.It has greatly practical significance and promotion value.

OBJECTIVE: To review the research progress and challenges of poly (L-lactic acid) (PLLA) membrane in preventing tendon adhesion. METHODS: The relevant literature at home and abroad in recent years was extensively searched, covering the mechanism of tendon adhesion formation, the adaptation challenge and balancing strategy of PLLA, the physicochemical modification of PLLA anti-adhesion membrane and its application in tendon anti-adhesion. In this paper, the research progress and modification strategies of PLLA membranes were systematically reviewed from the three dimensions of tissue adaptation, mechanical adaptation, and degradation adaptation. RESULTS: The three-dimensional adaptation of PLLA membrane is optimized by combining materials (such as hydroxyapatite, polycaprolactone), structural design (multilayer/gradient membrane), and drug loading (anti-inflammatory drug). The balance between anti-adhesion and pro-healing is achieved, the mechanical adaptation significantly improve, and degradation is achieved (targeting the degradation cycle to 2-4 weeks to cover the tendon repair period). CONCLUSION In the future, it is necessary to identify the optimal balance point of three-dimensional fitness, unify the evaluation criteria and solve the degradation side effects through the co-design of physicochemical modification and drug loading system to break through the bottleneck of clinical translation.
Tissue Adhesions/prevention & control* ; Polyesters/chemistry* ; Humans ; Biocompatible Materials/chemistry* ; Tendons/surgery* ; Membranes, Artificial ; Tendon Injuries/surgery* ; Wound Healing ; Animals ; Durapatite/chemistry*

Diabetic nephropathy(DN)is a significant causative factor of end-stage renal disease globally,and its pathogenesis involves dysregulation of multiple cellular and hormonal pathways.Podocytes play crucial roles in the process of DN,with the extent of podocyte injury closely associated with key pathological manifestations of renal damage,such as proteinuria,glomerular filtration rate,and glomerulosclerosis.However,due to the complexity and interplay of mechanisms contributing to podocyte injury,such as oxidative stress,abnormal lipid metabolism,and mitochondrial damage,the precise mechanisms underlying podocyte injury remain incompletely understood.This review integrated the latest research findings from both domestic and international studies on the core mechanisms of podocyte injury in DN.Furthermore,this article summarized the implications of these mechanisms for DN treatment,particularly focusing on potential therapeutic targets and the development of related pharmacological interventions derived from targeting podocyte injury pathways,so as to provide a theoretical foundation for the development of clinical therapeutic strategies for DN.

Dent disease is a rare X-linked recessive renal tubular disease characterized by low molecular weight proteinuria，hypercalcemia and nephrocalcinosis. It is also a major cause of tubular proteinuria in children. According to different causative genes，Dent disease can be divided into three types：type 1 is caused by mutations in the CLCN5 gene，accounting for about 60%-70%；type 2 is caused by mutations in the OCRL gene，accounting for about 15%-20%；type 3 has a similar clinical phenotype but no known pathogenic gene mutations. CLCN5 encodes the voltage-dependent 2Cl -/1H +exchange channel CIC-5，which is involved in proximal renal tubule endocytosis. Its mutations can cause a variety of proximal tubular dysfunction symptoms，mainly including low molecular weight proteinuria. The use of gene detection technology has resulted in an increase in reports on Dent disease year after year. At present，the specific mechanism underlying Dent disease remains unknown. This article reviews the research progress of CLCN5，hoping to provide new insight for the mechanism research of CLCN5 and the specific treatment of Dent disease type 1.