AIM To establish a UPLC-ESI-MS/MS method for analyzing the toxic material basis of 95％ethanol cold soaked ultrasonic extract(EC),95％ethanol heated reflux extract(EH)and water decoction extract(WD)from Dryopteris crassirhizoma Nakai.METHODS The analysis was performed on a 25 ℃ thermostatic agilent ZORBAX RRHD StableBond C18 column(2.1 mm×150 mm,1.8 μm),with the mobile phase comprising of methanol-0.2％formic acid flowing at 0.30 mL/min,and heated electrospray ion source was adopted in positive and negative ion scanning.Compounds were identified by Compound Discover 3.3 software combined with the database and related literature,and the main differential components were screened by Heatmap cluster analysis and partial least squares discriminant analysis.RESULTS 72 compounds were identified(22 phloroglucinols,19 flavonoids,8 phenylpropanoids,6 terpenoids and 17 other components).The main toxic differential components were phloroglucinols such as flavaspidic acid AB,didemethylpseudoaspidin AA and filixic acid PBP,flavonoids such as(-)-epicatechin,(-)-epigallocatechin,cianidanol,and other compounds such as indole-3-carboxaldehyde.CONCLUSION This method can rapidly,effectively and comprehensively characterize the main chemical composition of D.crassirhizoma,and provide a reference for the study of its pharmacological mechanism.

Bovine rotavirus(BRV)is an important pathogen causing diarrhea in calves.To understand the genomic charac-teristics and genetic variations in bovine rotavirus,and to further enrich data on the biological characteristics of rotavirus,we aimed to amplify 11 gene segments of the isolated and cultured G6P[1]bovine rotavirus BLL strain,perform whole genome se-quencing,and analyze the molecular characteristics.MEGA7.0 and DNAMAN software were used for homology and typing a-nalysis,and the whole genome phylogenetic tree was constructed to analyze genetic evolution relationships.The complete geno-type of the BLL strain was G6-P[1]-I2-R2-C2-M2-A3-N2-T6-E2-H3.Phylogenetic analysis of the VP7 and VP4 genes of the BLL strain showed that the VP7 gene had the highest homology with RVA/Cow-wt/HB01/China/2021,and the VP4 gene of the BLL strain was in the same branch as RVA/Human-tc/ISR/Ro8059/1995.From the sequence alignment of VP8*amino acids,the sialic acid domain of the BLL strain was found to be similar to that in other P[1]strains,but different from those in other types of strains,except for residue 189,which was the same as that in Ro8059 but different from that in other strains.The results suggested that the BLL strain might potentially infect humans.Therefore,continued monitoring and study of the biological characteristics of this strain are necessary to provide more information and evidence supporting further research on the cross-species transmission of group A rotavirus in China.

Accurate timing of myelination is crucial for the proper functioning of the central nervous system. Here, we identified a de novo heterozygous mutation in TMEM63A (c.1894G>A; p. Ala632Thr) in a 7-year-old boy exhibiting hypomyelination. A Ca²⁺ influx assay suggested that this is a loss-of-function mutation. To explore how TMEM63A deficiency causes hypomyelination, we generated Tmem63a knockout mice. Genetic deletion of TMEM63A resulted in hypomyelination at postnatal day 14 (P14) arising from impaired differentiation of oligodendrocyte precursor cells (OPCs). Notably, the myelin dysplasia was transient, returning to normal levels by P28. Primary cultures of Tmem63a^-/- OPCs presented delayed differentiation. Lentivirus-based expression of TMEM63A but not TMEM63A_A632T rescued the differentiation of Tmem63a^-/- OPCs in vitro and myelination in Tmem63a^-/- mice. These data thus support the conclusion that the mutation in TMEM63A is the pathogenesis of the hypomyelination in the patient. Our study further demonstrated that TMEM63A-mediated Ca²⁺ influx plays critical roles in the early development of myelin and oligodendrocyte differentiation.
Animals ; Cell Differentiation/physiology* ; Oligodendroglia/metabolism* ; Mice, Knockout ; Mice ; Male ; Myelin Sheath/metabolism* ; Humans ; Child ; Cells, Cultured ; Oligodendrocyte Precursor Cells/metabolism*

AIM To establish a UPLC-ESI-MS/MS method for analyzing the toxic material basis of 95％ethanol cold soaked ultrasonic extract(EC),95％ethanol heated reflux extract(EH)and water decoction extract(WD)from Dryopteris crassirhizoma Nakai.METHODS The analysis was performed on a 25 ℃ thermostatic agilent ZORBAX RRHD StableBond C18 column(2.1 mm×150 mm,1.8 μm),with the mobile phase comprising of methanol-0.2％formic acid flowing at 0.30 mL/min,and heated electrospray ion source was adopted in positive and negative ion scanning.Compounds were identified by Compound Discover 3.3 software combined with the database and related literature,and the main differential components were screened by Heatmap cluster analysis and partial least squares discriminant analysis.RESULTS 72 compounds were identified(22 phloroglucinols,19 flavonoids,8 phenylpropanoids,6 terpenoids and 17 other components).The main toxic differential components were phloroglucinols such as flavaspidic acid AB,didemethylpseudoaspidin AA and filixic acid PBP,flavonoids such as(-)-epicatechin,(-)-epigallocatechin,cianidanol,and other compounds such as indole-3-carboxaldehyde.CONCLUSION This method can rapidly,effectively and comprehensively characterize the main chemical composition of D.crassirhizoma,and provide a reference for the study of its pharmacological mechanism.

Bovine rotavirus(BRV)is an important pathogen causing diarrhea in calves.To understand the genomic charac-teristics and genetic variations in bovine rotavirus,and to further enrich data on the biological characteristics of rotavirus,we aimed to amplify 11 gene segments of the isolated and cultured G6P[1]bovine rotavirus BLL strain,perform whole genome se-quencing,and analyze the molecular characteristics.MEGA7.0 and DNAMAN software were used for homology and typing a-nalysis,and the whole genome phylogenetic tree was constructed to analyze genetic evolution relationships.The complete geno-type of the BLL strain was G6-P[1]-I2-R2-C2-M2-A3-N2-T6-E2-H3.Phylogenetic analysis of the VP7 and VP4 genes of the BLL strain showed that the VP7 gene had the highest homology with RVA/Cow-wt/HB01/China/2021,and the VP4 gene of the BLL strain was in the same branch as RVA/Human-tc/ISR/Ro8059/1995.From the sequence alignment of VP8*amino acids,the sialic acid domain of the BLL strain was found to be similar to that in other P[1]strains,but different from those in other types of strains,except for residue 189,which was the same as that in Ro8059 but different from that in other strains.The results suggested that the BLL strain might potentially infect humans.Therefore,continued monitoring and study of the biological characteristics of this strain are necessary to provide more information and evidence supporting further research on the cross-species transmission of group A rotavirus in China.

Objective:To investigate the correlation between peripheral blood T lymphocyte level and nerve function in patients with acute cerebral infarction.Methods:A total of 100 patients with acute cerebral infarction admitted to the First People′s Hospital of Xianyang from May 2020 to May 2023 were selected as the observation group. According to the degree of neurological impairment, they were divided into mild injury (33 cases), moderate injury (39 cases) and severe injury (28 cases). Another 60 healthy subjects in hospital during the same period were included as the control group. The levels of peripheral blood T lymphocyte subsets, including CD3 +, CD4 +, CD8 +, CD4 + /CD8 +, were detected. Spearman correlation coefficient was used to analyze the correlation between the level of peripheral blood T lymphocyte subsets and the degree of neurological impairment. Receiver operating characteristic (ROC) curve was drawn to analyze the value of T lymphocyte subsets in peripheral blood in the assessment of moderate and severe neurological impairment in acute cerebral infarction. Multivariate logistic stepwise regression was used to analyze the influencing factors of severe neurological impairment in acute cerebral infarction. Results:The levels of CD3 +, CD4 +, CD4 + /CD8 + in observation group were lower than those in control group, and CD8 + levels were higher than those in control group (all P<0.05). The levels of CD3 +, CD4 +, CD4 + /CD8 + were gradually decreased and CD8 + levels were gradually increased in patients with mild, moderate and severe neurological impairment (all P<0.05). Spearman correlation analysis showed that the levels of CD3 +, CD4 +, CD4 + /CD8 + were negatively correlated with the degree of neurological impairment in patients with acute cerebral infarction, and CD8 + was positively correlated with the degree of neurological impairment (all P<0.05). According to ROC curve analysis, area under the curve (AUC) of CD3 +, CD4 +, CD8 + and CD4 + /CD8 + in evaluating severe neurological impairment in acute cerebral infarction was 0.808, 0.874, 0.833 and 0.782, respectively, and the sensitivity was 0.791, 0.821, 0.806 and 0.716, respectively. The specificity was 0.758, 0.818, 0.788, 0.727 (all P<0.05), respectively. Compared with mild defect patients, the proportion of patients with moderate and severe defect complicated with diabetes mellitus, cardioembolism in Toast classification and infarct size >3 cm 2 was higher (all P<0.05). Multivariate logistic regression analysis showed that combined diabetes, Toast classification, infarct size, CD3 +, CD4 +, CD8 +, CD4 + /CD8 + levels were the influential factors for moderate and severe neurological impairment in acute cerebral infarction (all P<0.05). Conclusions:Compared with the normal population, the levels of CD3 +, CD4 +, CD4 + /CD8 + in patients with acute cerebral infarction are lower, and the level of CD8 + is higher, and the level of T lymphocytes is correlated with the degree of neurological impairment in patients, which has certain reference value for evaluating the severity of the disease.

MicroRNAs(miRNAs)are small non-coding RNAs that are closely associated with the occur-rence and progression of tumors and other diseases.However,miRNAs require high-sensitivity and high-specificity detection due to their low abundance,high sequence homology,and rapid degradation.The RNA-cleaving deoxyribozyme(RCD)is a functional single-stranded DNA molecule that enables specific cleavage of substrates to release miRNAs that can be recycled with the assistance of metal ions,prompting cyclic signal amplification.Recently,developing new methods based on RCD catalytic amplification for miRNA high-sensitivity detection has become the focus of researchers.Based on combing with the various new technologies and materials in miRNA biosensors,this study classifies and reviews the new methods for detecting miRNAs based on deoxyribozyme catalytic amplification developed in recent years.We sepa-rate these miRNA detection strategies into three categories:RCDs combined with DNA self-assembly,i-sothermal amplification,and nanomaterials.We explore the basic principles of each approach,the latest research advancements,and application scenarios in biomedical sensors and medical detection.This re-view provides a foundation and reference for further research of highly sensitive and accurate miRNA de-tection strategies.

Objective:To investigate the correlation between peripheral blood T lymphocyte level and nerve function in patients with acute cerebral infarction.Methods:A total of 100 patients with acute cerebral infarction admitted to the First People′s Hospital of Xianyang from May 2020 to May 2023 were selected as the observation group. According to the degree of neurological impairment, they were divided into mild injury (33 cases), moderate injury (39 cases) and severe injury (28 cases). Another 60 healthy subjects in hospital during the same period were included as the control group. The levels of peripheral blood T lymphocyte subsets, including CD3 +, CD4 +, CD8 +, CD4 + /CD8 +, were detected. Spearman correlation coefficient was used to analyze the correlation between the level of peripheral blood T lymphocyte subsets and the degree of neurological impairment. Receiver operating characteristic (ROC) curve was drawn to analyze the value of T lymphocyte subsets in peripheral blood in the assessment of moderate and severe neurological impairment in acute cerebral infarction. Multivariate logistic stepwise regression was used to analyze the influencing factors of severe neurological impairment in acute cerebral infarction. Results:The levels of CD3 +, CD4 +, CD4 + /CD8 + in observation group were lower than those in control group, and CD8 + levels were higher than those in control group (all P<0.05). The levels of CD3 +, CD4 +, CD4 + /CD8 + were gradually decreased and CD8 + levels were gradually increased in patients with mild, moderate and severe neurological impairment (all P<0.05). Spearman correlation analysis showed that the levels of CD3 +, CD4 +, CD4 + /CD8 + were negatively correlated with the degree of neurological impairment in patients with acute cerebral infarction, and CD8 + was positively correlated with the degree of neurological impairment (all P<0.05). According to ROC curve analysis, area under the curve (AUC) of CD3 +, CD4 +, CD8 + and CD4 + /CD8 + in evaluating severe neurological impairment in acute cerebral infarction was 0.808, 0.874, 0.833 and 0.782, respectively, and the sensitivity was 0.791, 0.821, 0.806 and 0.716, respectively. The specificity was 0.758, 0.818, 0.788, 0.727 (all P<0.05), respectively. Compared with mild defect patients, the proportion of patients with moderate and severe defect complicated with diabetes mellitus, cardioembolism in Toast classification and infarct size >3 cm 2 was higher (all P<0.05). Multivariate logistic regression analysis showed that combined diabetes, Toast classification, infarct size, CD3 +, CD4 +, CD8 +, CD4 + /CD8 + levels were the influential factors for moderate and severe neurological impairment in acute cerebral infarction (all P<0.05). Conclusions:Compared with the normal population, the levels of CD3 +, CD4 +, CD4 + /CD8 + in patients with acute cerebral infarction are lower, and the level of CD8 + is higher, and the level of T lymphocytes is correlated with the degree of neurological impairment in patients, which has certain reference value for evaluating the severity of the disease.

This study collected epidemic data of COVID-19 in Zhengzhou from January 1 to January 20 in 2022. The epidemiological characteristics of the local epidemic in Zhengzhou High-tech Zone caused by the SARS-CoV-2 Delta variant were analyzed through epidemiological survey and big data analysis, which could provide a scientific basis for the prevention and control of the Delta variant. In detail, a total of 276 close contacts and 599 secondary close contacts were found in this study. The attack rate of close contacts and secondary close contacts was 5.43% (15/276) and 0.17% (1/599), respectively. There were 10 confirmed cases associated with the chain of transmission. Among them, the attack rates in close contacts of the first, second, third, fourth and fifth generation cases were 20.00% (5/25), 17.86% (5/28), 0.72% (1/139) and 14.81% (4/27), 0 (0/57), respectively. The attack rates in close contacts after sharing rooms/beds, having meals, having neighbor contacts, sharing vehicles with the patients, having same space contacts, and having work contacts were 26.67%, 9.10%, 8.33%, 4.55%, 1.43%, and 0 respectively. Collectively, the local epidemic situation in Zhengzhou High-tech Zone has an obvious family cluster. Prevention and control work should focus on decreasing family clusters of cases and community transmission.
Humans ; SARS-CoV-2 ; COVID-19 ; Epidemics ; Incidence

Objective:To explore the changes of hip joint function in patients with total hip arthroplasty (THA) within three months after operation, and to explore the impact of fear of falling on hip joint function recovery.Methods:From December 2020 to August 2021, 205 patients with THA after operation in the Xinjiang Uygur Municipal People's Hospital were selected as the study subject by objective sampling. The patients were investigated with the General Information Questionnaire, the Chinese version of Falls Efficacy Scale-International (FES-I) , and the Harris Hip Score after the first activity out of bed, one month and three months after operation. Pearson correlation was used to analyze the correlation between the scores of FES-I in Chinese version and the scores of Harris Hip Score in different periods after operation, and multiple linear regression were used to analyze the influencing factors of hip joint function recovery in different periods after operation.Results:After the first activity out of bed, one month and three months after operation, the FES-I scores of 205 patients after THA were (32.24±6.05) , (30.94±6.04) and (28.48±3.52) respectively, and the scores of Harris Hip Score were (61.49±8.92) , (72.58±5.52) and (89.15±5.70) respectively, and the correlation analysis showed that they were negatively correlated ( r=-0.512, -0.523, -0.549; P<0.05) . The results of multiple linear regression analysis showed that age and fear of falling were the influencing factors for the hip joint function recovery when THA patients got out of bed for the first time after operation ( P<0.05) , which could explain 31.2% of the total variation. Sex, age, number of concomitant diseases and fear of falling were the influencing factors of hip joint function recovery in THA patients one month after operation ( P<0.05) , which could explain 43.7% of the total variation. Sex, age, number of concomitant diseases and fear of falling were the influencing factors of hip joint function recovery in THA patients three months after operation ( P<0.05) , which could explain 36.9% of the total variation. Conclusions:The recovery of hip joint function in THA patients shows an overall upward trend three months after operation. The impact of fear of falling on the recovery of hip joint function in different periods persists. The higher the level of fear of falling, the worse the recovery of hip joint function. Nurses should evaluate the patients' fear of falling at different periods after operation, and strengthen the attention and guidance to patients with high fear of falling, elderly patients, and patients with other diseases, so as to promote the recovery of patients' joint function.