Objective:To explore the intervention effects of the "four-steps tendon resetting and collaterals dredging manipulation" on the symptoms and muscle status of calf muscle group of patients with chronic ankle sprains.Methods:This study was a prospective self-controlled clinical trial. A total of 39 patients with chronic ankle sprains who sought treatment at the basic units from April to September 2023 and Rehabilitation Medicine Center of Characteristic Medical Center of PLA Rocket Force from February to October 2023 were recruited. The "four-steps tendon resetting and collaterals dredging manipulation" was employed for treatment, with two sessions conducted per patient and one session per week. Pain levels were assessed using the Visual Analog Scale (VAS), ankle joint function was evaluated using the Ankle Osteoarthritis Scale and Function Assessment (AOFAS) score, and MyotonPRO digital palpation instrument was used to measure bilateral triceps and peroneal longus muscles and evaluate muscle status.Results:Compared with before treatment, VAS scores decreased ( t values were 5.85, 5.97, respecively, P<0.001) and AOFAS scores increased ( Z values were -4.59, -4.68, respecively, P<0.001). Before the first treatment and after the second treatment, the damping vibration frequency (Freq) of the affected and healthy triceps and peroneal muscles increased ( t values were -3.09,-2.92,-2.97,-2.28, respecively, P<0.05), the muscle stiffness (Stiff) increased ( t values were -3.12, -2.99, -2.88, -2.15, respecively, P<0.05), and the logarithmic attenuation value (Decr) of the damping vibration of the healthy calf triceps muscle decreased ( t=-2.31, P<0.05); Compared before and after the first treatment, the Decr value ( t=-2.51, P<0.05) and Stiff value ( t=-2.05, P<0.05) of the affected fibular longus muscle increased, while the Ferq, Decr, and Stiff values of the healthy calf triceps muscle increased before and after treatment ( t values were -2.92, -2.13, -2.64, respecively, P<0.05); before and after the second treatment, the Freq values of the triceps and peroneal longus muscles on the affected and healthy sides increased ( t values were -4.28, -2.67, -2.69, -2.38, respecively, P<0.05) and Stiff values increased ( t values were -4.24, -3.43, -3.87, -2.33, respecively, P<0.05); there was no statistical significance in Ferq, Decr, and Stiff values between the affected and healthy sides before and after the first and second treatments ( P>0.05). Conclusion:The "four-steps tendon resetting and collaterals dredging manipulation" can improve the symptoms of chronic ankle sprains and significantly change the muscle condition of the affected and healthy sides of the calf. The mechanism may be related to the neuromuscular control mechanism.

Objective:To summarize the risk prediction models for postoperative hypoxemia and provide a reference for clinical nursing practice and future research on hypoxemia risk prediction models for postoperative patients.Methods:A systematic literature search was conducted in CNKI, CBM, Wanfang, PubMed, Web of Science Core Collection, Cochrane Library, Embase, and CINAHL databases, covering publications up to January 31, 2024. Two researchers independently screened the literature, extracted data, performed integrative analysis, and evaluated the risk of bias in the included studies.Results:Seventeen studies were included, involving 17 different prediction models. The study populations were primarily adult patients, with hypoxemia incidence rates ranging from 2.40% to 49.30%. Modeling methods included Logistic regression and decision tree algorithms. The presentation formats of the models included risk scoring formulas, nomograms, decision tree diagrams, and web calculators. The five most frequently identified predictive factors were body mass index, age, comorbidities, duration of intraoperative cardiopulmonary bypass, and preoperative white blood cell count. Sixteen models reported the area under the receiver operating characteristic curve ranging from 0.667 to 0.916. All 17 studies exhibited varying degrees of bias risk.Conclusions:Existing risk prediction models for postoperative hypoxemia demonstrate good performance; however, the bias risk level of all studies was high. Future research should standardize the model development process according to bias risk assessment checklists to establish models with low bias risk and strong clinical applicability.

This article provides an overview of ecological momentary assessment based on mobile information devices. It reviews the design frameworks, domestic and international research progress, and the challenges associated with its application. The study aims to offer insights and reference methods for developing electronic ecological momentary assessment platforms in China.

Objective:To summarize the risk prediction models for postoperative hypoxemia and provide a reference for clinical nursing practice and future research on hypoxemia risk prediction models for postoperative patients.Methods:A systematic literature search was conducted in CNKI, CBM, Wanfang, PubMed, Web of Science Core Collection, Cochrane Library, Embase, and CINAHL databases, covering publications up to January 31, 2024. Two researchers independently screened the literature, extracted data, performed integrative analysis, and evaluated the risk of bias in the included studies.Results:Seventeen studies were included, involving 17 different prediction models. The study populations were primarily adult patients, with hypoxemia incidence rates ranging from 2.40% to 49.30%. Modeling methods included Logistic regression and decision tree algorithms. The presentation formats of the models included risk scoring formulas, nomograms, decision tree diagrams, and web calculators. The five most frequently identified predictive factors were body mass index, age, comorbidities, duration of intraoperative cardiopulmonary bypass, and preoperative white blood cell count. Sixteen models reported the area under the receiver operating characteristic curve ranging from 0.667 to 0.916. All 17 studies exhibited varying degrees of bias risk.Conclusions:Existing risk prediction models for postoperative hypoxemia demonstrate good performance; however, the bias risk level of all studies was high. Future research should standardize the model development process according to bias risk assessment checklists to establish models with low bias risk and strong clinical applicability.

This article provides an overview of ecological momentary assessment based on mobile information devices. It reviews the design frameworks, domestic and international research progress, and the challenges associated with its application. The study aims to offer insights and reference methods for developing electronic ecological momentary assessment platforms in China.

ObjectiveTo explore the effects and mechanisms of Naotai Formula （脑泰方） against white matter lesions associated with cerebrovascular disease via Wnt/β-catenin pathway. MethodsTen WKY rats were used as sham surgery group， and 30 spontaneously hypertensive rats （SHRs） were randomly divided into model group， low-and high-dose Naotai Formula group， with 10 rats in each group. In addition to the sham surgery group， rats in each group were modelled by dorsum subcutaneous injection of D-galactose for 56 days， by performing bilateral common carotid artery stenosis on the 29th day of modelling with low perfusion for 28 days to construct the rat models of cerebral small-vessel disease with composite risk factors. The rats in the low- and high-dose Naotai Formula groups were given 9 and 27 g/（kg·d） of Naotai Formula by gavage on the day after surgery， and 10 ml/（kg·d） of pure water by gavage in the sham surgery group and the model group， with all rats in each group gavaged for 4 weeks. Blood pressure of rats was monitored weekly before and after medication administration； water maze experiments were performed for 6 consecutive days from the 50th day of modelling； and changes in cerebral blood flow were detected by laser scattering preoperatively， postoperatively， and after medication administration. The samples were taken on the next day after the last gavage， and the extent of myelin damage in the rat corpus callosum was observed by Lucas fast blue （LFB） and transmission electron microscopy， and the levels of interleukin 1β （IL-1β）， tumour necrosis factor α （TNF-α）， interleukin 10 （IL-10）， and tumour necrosis factor β （TNF-β） were detected by ELISA， and the levels of expression of myelin basic protein （MBP） and ionized calcium binding adaptor molecule 1 （IBA1）， neuroglial antigen 2 （NG2）， cell proliferation marker Ki67 antigen （MBP）， and cell proliferation marker Ki67 antigen （Ki67A）/NG2， 2'，3'- cyclic-nucleotide 3'-phosphodiesterase （CNPase） expression were measured by immunofluorescence. MBP in the corpus callosum， wingless MMTV integration site family member 3a （Wnt3a）， phosphorylated glycogen synthase kinase 3β （p-GSK-3β）， glycogen synthase kinase 3β （GSK-3β）， phosphorylated β-catenin （p-β-catenin）， β-catenin expression were detected by western blotting， and GSK-3β， β-catenin mRNA expression in the corpus callosum were detected by RT-qPCR. ResultsCompared with the sham surgery group， rats in the model group had elevated systolic blood pressure at all time points， decreased the number of traversing platforms and the percentage of time spent in the target quadrant in the water maze experiments， significantly prolonged the evasion latency on days 4 and 5， and decreased cerebral blood flow in the postoperative period and after medication administration （P＜0.05 or P＜0.01）； significant myelin sheath damage in the corpus callosum was seen by LFB staining and transmission electron microscopy； the callus had significantly higher levels of TNF-α and IL-1β content increased， TNF-β content decreased， MBP fluorescence intensity and protein expression decreased， the number of IBA1-positive cells increased， Wnt3a and p-GSK-3β/GSK-3β values increased， p-β-catenin/β-catenin values decreased， GSK-3β mRNA expression decreased， and β-catenin mRNA expression increased （P＜0.05 or P＜0.01）. Compared with the model group， the myelin sheath damage of corpus callosum in rats in the high-dose Naotai Formula groups was significantly improved， and all of the above indexes were significantly improved （P＜0.05 or P＜0.01）. Compared with the low-dose Naotai Formula group， myelin damage in the corpus callosum of the high-dose Naotai Formula group was improved， systolic blood pressure reduced， cerebral blood flow and MBP fluorescence intensity and protein expression in the corpus callosum， the number of positive cells for NG2 and CNPase， the values of p-β-catenin/β-catenin and GSK-3β mRNA expression increased， and the expression of Wnt3a， p-GSK-3β/ GSK-3β values and β-catenin mRNA expression decreased （P＜0.05 or P＜0.01）. ConclusionsHigh-dose Naotai Formula can significantly improve white matter lesions in cerebral small vessel disease， and the mechanism may be related to the inhibition of the Wnt/β-catenin signalling pathway， reducing the inflammatory microenvironment in the brain， and promoting the differentiation of oligodendrocyte precursor cells into oligodendrocytes.

Objective:To summarize the best evidence for preventing and managing radiotherapy-induced oral mucositis in patients with head and neck cancer.Methods:The clinical decisions, best practices, guidelines, expert consensus, systematic reviews, and evidence summaries regarding the prevention and management of radiotherapy-induced oral mucositis in patients with head and neck cancer were retrieved from UpToDate, British Medical Journal (BMJ) Best Practice, Agency for Healthcare Research and Quality, Medlive, National Comprehensive Cancer Network, European Society for Medical Oncology, Cochrane Library, Joanna Briggs Institute (JBI) Evidence-Based Health Care Center Database, PubMed, Embase, Web of Science, China National Knowledge Infrastructure, WanFang Data, China Biology Medicine disc and so on. The search period was from database establishment to November 30, 2023.Results:A total of 18 articles were included, involving six guidelines, two expert consensus, eight systematic reviews, and two evidence summaries. Thirty-four best pieces of evidence were summarized from six aspects of assessment: drug prevention, non-drug prevention, anti-infection and analgesic management, health education, and multidisciplinary team management.Conclusions:This study summarizes the best evidence for preventing and managing radiotherapy-induced oral mucositis in patients with head and neck cancer. Medical and nursing staff should consider the patient's characteristics, disease condition, and willingness when selecting and applying evidence.

Objective:To explore the regulatory mechanism of α-synuclein in the degradation of autophagy-lysosome pathway(ALP) in Parkinson disease(PD) model cells after interference or overexpression of dynein heavy chain(Dynhc) gene.Methods:SH-SY5Y cells were divided into control group, PD group, Dynhc interference group, Dynhc overexpression group, and Dynhc interference+ rapamycin group according to experimental requirements.Using Western blot to detect Dynhc, α-synuclein, microtubule-associated protein l light chain 3 (LC3), lysosome-associated membrane protein 2 (LAMP2), tubulin, dynein activator protein p150, and kinesin KIF5B.Flow cytometry was used to detect the level of cell apoptosis.Immunoconfocal microscopy was used to observe the structure of tubulin and the co-localization of LC3 and LAMP.SPSS 23.0 software was used for statistical analysis.One-way ANOVA was used for inter group comparisons, and further pairwise comparisons were conducted by LSD- t test. Results:There were statistically significant differences in the expression of α-synuclein, autophagy-related proteins, microtubules, and microtubule-related proteins among cells in the 5 groups(all P<0.001). The protein expression levels of Dynhc, α-synuclein, LC3, LAMP2, p150, and KIF5B in the PD group were higher than those in the control group (all P<0.05). The protein levels of Dynhc, LAMP2, tubulin and p150 in the Dynhc interference group were lower than those in the PD group (all P<0.05), while the protein levels of α-synuclein, LC3 and KIF5B were higher than those in the PD group (all P<0.05). The protein levels of α-synuclein, LC3, and KIF5B in the Dynhc overexpression group were lower than those in the PD group (all P<0.05), while the protein levels of Dynhc, LAMP2 and p150 were higher than those in the PD group (all P<0.05). The protein level of LC3 in the Dynhc interference+ rapamycin group was higher than that in the Dynhc interference group ( P<0.05). There were no statistically significant differences in the protein levels of Dynhc, α-synuclein, LAMP2, microtubule protein, p150 and KIF5B compared to the Dynhc interference group (all P>0.05). Compared with the control group, the cell apoptosis rate in PD group increased((12.77±1.66)%, (7.64±1.45)%), the microtubule morphology remained unchanged, and autophagosomes fused more with lysosomes. Compared with the PD group, the cell apoptosis rate of Dynhc overexpression group decreased, and there was no significant change in microtubule structure, and there was more fusion between autophagosomes and lysosomes.Compared with the PD group, the cell apoptosis rat of Dynhc interference group increased((18.45±1.91)%), and the microtubule morphology was sparse, and there was less fusion between autophagosomes and lysosomes. Compared with the PD group, the Dynhc overexpression group showed a decrease in cell apoptosis rate ((9.95±1.56)%), no significant changes in microtubule structure, and more fusion between autophagosomes and lysosomes.Compared with the Dynhc interference group, the Dynhc interference+ rapamycin group showed no significant changes in cell apoptosis rate ((19.05±2.46)%), microtubule morphology, and fusion of autophagosomes and lysosomes. Conclusion:Dynhc can reduce cell apoptosis by enhancing cell ALP function, increasing the degradation of α-synuclein and maintaining of microtubule structure integrity.

Objective:To investigate the safety and effectiveness of Neuroform Atlas stent-assisted coiling in the endovascular treatment of intracranial aneurysms.Methods:This was a retrospective, single-center observational study of 77 patients who underwent endovascular treatment of intracranial aneurysms using the Neuroform Atlas device at the Department of Neurology, People′s Liberation Army General Hospital from July 2020 to May 2022. There were 34 males and 43 females, with a median (range) age of 59 (23-81) years. The degree and effect of aneurysm embolization were evaluated by modified Raymond grading post procedure and after 6 months. Complications occurring during the perioperative period were recorded. Vaso computed tomography was performed immediately after the operation to assess stent opening and adherence. Digital subtraction angiography was performed 6 months after discharge and the aneurysm was classified as cured, stable, or recurrent.Results:A total of 87 Atlases were successfully released in 77 cases. Angiography performed immediately after the embolization revealed 19 (24.7%) modified Raymond grade Ⅰ, 10 (13.0%) grade Ⅱ, and 48 (62.3%) grade Ⅲa cases. Three perioperative complications were observed including thrombotic events in 2 cases and stent migration in 1 case. A follow-up angiogram was available for 47 aneurysms, and showed that modified Raymond grade Ⅰ occlusion was achieved in 38 (80.9%) cases, grade Ⅱ in 2 (4.3%) cases, and grade Ⅲa in 7 (14.9%) cases. At the 6-month follow-up, 38 patients were cured and 7 were stable, whereas 2 patients experienced a recurrence of aneurysm. Stenosis of the parent artery occurred in 3 (6.4%) cases, including 2 at the head and 1 inside the stent.Conclusions:The results of this preliminary study suggest that Neuroform Atlas stent-assisted coiling has a high occlusion rate and low incidence of complications in the endovascular treatment of aneurysms. However, the effectiveness of this procedure for large aneurysms and long-term outcomes require further investigation.

Objective:To understand the genome analysis and molecular typing of foodborne Yersinia enterocolitica ( Y.e) strains in Liaocheng City of Shandong Province from 2020 to 2021. Methods:The Y.e strains were isolated from raw meat and meat products. Then we made the strain identification, drug sensitivity test, virulence gene test, pulsed-field gel electrophoresis (PFGE), and whole genome sequencing (WGS). The genome sequencing data were assembled with the microbial genome annotation package. We performed the multilocus sequence typing (MLST) and core genome multilocus sequence typing (cgMLST) and used WGS-based single nucleotide polymorphism typing (wg-SNPs) method to carry out genetic evolution analysis with 14 domestic and Y.e genomes obtained from the NCBI. Results:A total of 21 strains of Y.e were detected from 165 samples, with a detection rate of 12.73%. The 20 strains of Y.e were sequenced successfully. The 20 strains of Y.e carries a variety of drug resistance genes and virulence genes, showing multiple drug resistance. The virulence gene PCR test showed that 21 strains of Y.e having two virulence genes. Cluster analysis of PFGE, MLST, and cgMLST showed that the genomics of 21 strains was highly diverse. The genetic evolution analysis of wg-SNPs showed that 20 Y.e strains could be divided into two main evolutionary branches. Conclusions:Y.e strains isolated from raw meat in Liaocheng City carry a variety of drug resistance genes and virulence genes, and the molecular typing is highly diverse, which may cause infection risk. The molecular biological monitoring of Y.e in raw meat should be strengthened, and genome sequencing and molecular typing detection be carried out to provide the theoretical basis for foodborne illness caused by Y.e.