This study aims to understand the epidemic distribution characteristics,antimicrobial resistance,virulence genes,and biofilm adhesion ability of Enterococcus in yaks on the Tibetan plateau.Three hundred and forty-six fresh yak fecal samples and 311 milk samples were collected from four provinces on the Tibetan plateau(Xizang,Sichuan,Gansu,Qinghai),totaling 657 sam-ples.Bacterial isolation and identification were conducted,followed by 16S rDNA gene detection and the construction of a systematic evolutionary tree.The isolated strains were tested for antimi-crobial resistance and virulence genes using PCR,and sensitivity tests were performed using 18 types of antibiotics.The biofilm adhesion ability of the isolated bacteria was determined using an improved semi-quantitative crystal violet staining method.The results showed that the total isola-tion rate of Enterococcus was 32.27％,with Sichuan having the highest at 60.23％,followed by Gansu,Qinghai,and Tibet autonomous region at 42.70％,23.47％,and 18.31％respectively.In terms of sample types,the isolation rate in fecal samples was 36.71％,and in milk samples,it was 27.33％.Through PCR amplification,bands of approximately 1 400 bp were obtained,and 5 strains were selected for evolutionary analysis,forming a separate cluster.Among the 212 isolated strains,a high resistance to clindamycin,quinupristin-dalfopristin,linezolid,levofloxacin,and erythromycin was observed,with various resistance phenomena,accounting for 60.85％.Only 5 out of 12 resist-ant genes were detected,namely erm(B),tet(L),tet(O),tet(M),and ant(6)-Ia.All 13 virulence genes were detected in Enterococcus,with detection rates in the range of 5.19％to 95.76％,where cpd was 95.75％,gelE was 91.98％,efaA was 86.79％,asal was 86.32％,and the rest ranged from 5.19％to 55.66％.The fsr virulence gene was not detected in Enterococcus from milk sources.Among the isolated strains,3.30％showed medium adhesive ability,48.58％showed weak adhesive ability,and 48.11％showed no adhesive ability.The above research revealed the preva-lence of yak derived Enterococcus,the carrying status of resistance and virulence genes,and the correlation between biofilm phenotypes,laying the foundation for mastering research data on yak-derived Enterococcus in the Tibetan plateau.

This study aims to understand the epidemic distribution characteristics,antimicrobial resistance,virulence genes,and biofilm adhesion ability of Enterococcus in yaks on the Tibetan plateau.Three hundred and forty-six fresh yak fecal samples and 311 milk samples were collected from four provinces on the Tibetan plateau(Xizang,Sichuan,Gansu,Qinghai),totaling 657 sam-ples.Bacterial isolation and identification were conducted,followed by 16S rDNA gene detection and the construction of a systematic evolutionary tree.The isolated strains were tested for antimi-crobial resistance and virulence genes using PCR,and sensitivity tests were performed using 18 types of antibiotics.The biofilm adhesion ability of the isolated bacteria was determined using an improved semi-quantitative crystal violet staining method.The results showed that the total isola-tion rate of Enterococcus was 32.27％,with Sichuan having the highest at 60.23％,followed by Gansu,Qinghai,and Tibet autonomous region at 42.70％,23.47％,and 18.31％respectively.In terms of sample types,the isolation rate in fecal samples was 36.71％,and in milk samples,it was 27.33％.Through PCR amplification,bands of approximately 1 400 bp were obtained,and 5 strains were selected for evolutionary analysis,forming a separate cluster.Among the 212 isolated strains,a high resistance to clindamycin,quinupristin-dalfopristin,linezolid,levofloxacin,and erythromycin was observed,with various resistance phenomena,accounting for 60.85％.Only 5 out of 12 resist-ant genes were detected,namely erm(B),tet(L),tet(O),tet(M),and ant(6)-Ia.All 13 virulence genes were detected in Enterococcus,with detection rates in the range of 5.19％to 95.76％,where cpd was 95.75％,gelE was 91.98％,efaA was 86.79％,asal was 86.32％,and the rest ranged from 5.19％to 55.66％.The fsr virulence gene was not detected in Enterococcus from milk sources.Among the isolated strains,3.30％showed medium adhesive ability,48.58％showed weak adhesive ability,and 48.11％showed no adhesive ability.The above research revealed the preva-lence of yak derived Enterococcus,the carrying status of resistance and virulence genes,and the correlation between biofilm phenotypes,laying the foundation for mastering research data on yak-derived Enterococcus in the Tibetan plateau.

Objective:To evaluate the clinical value of tissue doppler imaging Tei index in evaluating the degree of myocardial injury, right cardiac function after neonatal asphyxia.Methods:From March 2018 to May 2019, 62 cases of neonatal asphyxia hospitalized in the undergraduate department of Yiwu Central Hospital were classified as asphyxia group.According to Apgar score of birth, they were further divided into 41 cases of mild asphyxia group, 21 cases of severe asphyxia group.And 30 healthy full-term neonates delivered in our Hospital during the same period were selected as normal control group.The Newborn's Tei index, as well as the different severity asphyxia neonatal serum myocardial injury indicators[amino terminal brain natriuretic peptide(NT-proBNP), troponin(cTn-Ⅰ), creatine kinase isoenzyme(CK-MB), lactate dehydrogenase(LDH)], ultrasonic right heart function parameters[right ventricular ejection fraction(RVEF), E, A value and E/A ratio] were compared.Pearson test was used to evaluate the correlation between Tei index, myocardial injury indicators and right cardiac function parameters in neonatal asphyxia cases.Results:The Tei index of the asphyxiation group was (0.38±0.05), which was higher than (0.27±0.04) of the control group ( t=10.521, P<0.05), and the Tei index of the severe asphyxiation group was (0.43±0.06), which was higher than (0.34±0.05) of the mild asphyxiation group ( t=6.264, P<0.05). In neonatal asphyxia cases, the NT-proBNP, cTnI, CK-MB, LDH levels in the severe asphyxia group were (1 164.27±231.64)ng/L, (0.33±0.05)μg/L, (27.11±3.65)U/L, (298.20±37.57)U/L, respectively, which were higher than those in the mild asphyxia group [(590.38±73.91)ng/L, (0.25±0.04)μg/L, (18.36±2.34)U/L, (200.71±24.39)U/L] ( t=14.576, 6.839, 11.463, 12.338, all P<0.05). The ultrasonic RVEF level, E/A ratio in the severe asphyxia group were (52.94±6.10)%, (0.94±0.11), respectively, which were lower than those in the mild asphyxia group [(56.83±5.97)%, (1.02±0.13)] ( t=2.411, 2.547, all P<0.05). Correlation analysis found that Tei index was positively correlated with NT-proBNP, cTn-Ⅰ, CK-MB, LDH levels, and negatively correlated with RVEF level, and positively correlated with E/A ratio in neonatal asphyxia cases( r=0.745, 0.598, 0.703, 0.665, -0.711, -0.692, all P<0.05). Conclusion:Abnormal increasing of Tei index in neonatal asphyxia cases can objectively reflect the extent of myocardial injury and right heart function decline in children.

Aged ; Benzylisoquinolines ; therapeutic use ; Bronchoalveolar Lavage ; Female ; Humans ; Male ; Middle Aged ; Oxidative Stress ; Phytotherapy ; Pneumoconiosis ; metabolism ; therapy ; Quality of Life ; Treatment Outcome

Objective To investigate the effect of non-accompany nursing on mood of caesarean section maternal. Methods A total of 320 caesarean section maternal were selected and divided into the observation group and the con-trol group with each group 160 cases. The both groups were given routine nursing. But the daily life nursing in the control group was performed by family members, the daily life nursing in the observation group was performed by hos-pital escort. The self-rating anxiety scale(SAS), salf-rating depression scale(SDS) were and satisfaction at 1 d before surgery and 6 d after surgery in two groups were recorded and compared. Results The SAS scores in two group at 1d before surgery were similiar. The difference was not statistically significant(P>0.05). But the SAS score of the observa-tion group(45.98±1.94) were lower than that of the control group(49.15±2.88), the difference was statistically signifi-cant(P<0.05). The SDS scores in two group at 1 d before surgery were similiar. The difference was not statistically significant(P>0.05). But the SDS score of the observation group(46.15±2.25)were lower than that of the control group(51.74±3.09), the difference was statistically significant(P<0.05). The satisfaction of the observation group was better than that of the control group. The difference was statistically significant(P<0.05). Conclusion Implementing the non-accompany nursing can effectively improve reduce the anxiety and depression of caesarean section maternal, contribute to improve the quality of rehabilitation.