Objective To explore the clinical effect of cold treatment of replacement fluid in continuous blood purification(CBP)of patients with heat stroke.Methods Clinical data of 46 patients with heat stroke who were treated with CBP in Hainan Hospital of PLA General Hospital from July 2018 to September 2022 were retrospectively analyzed.The patients were assigned to control group(23 cases,conventional treatment for heat stroke and CBP with room temperature replacement fluid)and observation group(23 cases,conventional treatment for heat stroke and CBP with cooling replacement fluid).The body temperatures were compared between the two groups before treatment and 30 min,2 h,6 h,12 h and 24 h after treatment.The prothrombin activity(PTA),activated partial thromboplastin time(APTT),D-dimer(D-D),fibrinogen(FIB)and platelet count(PLT)before treatment and at 24 h(T1),3 d(T2)and 7 d(T3)after treatment,as well as the Acute Physiology and Chronic Health Evaluation Ⅱ(APACHEⅡ)score at 7 d after treatment were also compared between the two groups.Results There was no significant difference in temperature or coagulation function between the two groups before treatment(P>0.05).The temperature of the observation group was significantly different from that of the control group at 2 h,6h and 12h after treatment(P<0.05).After treatment,the levels of PTA,FIB and PLT in the observation group at T1,T2 and T3 were higher than those in the control group,while the level of D-D in the observation group was lower than that in the control group,and the APPT at T1 and T2 was significantly shorter than that in the control group(P<0.05).PTA,APPT,D-D,FIB and PLT of the two groups were improved after treatment(P<0.05).Conclusion The cold treatment of replacement fluid can quickly shorten the cooling time of patients with heat stroke during CBP,and significantly improve coagulation function.It is worthy of clinical promotion so as to improve the progrosis of patients with heat strok.

Crown pulp regeneration is a method to replace the pulp-capping agent with stem cells and scaffold structures, which are placed on the section of healthy root pulp tissue after pulpotomy to regenerate the pulp-dentin complex in the crown. This paper reviews the significance, physiological basis, application and challenges of crown pulp regeneration to provide new ideas for the study of pulp regeneration. The results of a literature review show that the combination of traditional pulpotomy, stem cell biology and tissue engineering, as well as the regeneration of crowns and pulp by using the tissue repair potential of healthy root pulp can effectively promote the regeneration of dentin and parts of pulp. In recent years, with the development of research on pulp regeneration, many challenges have been gradually revealed. It is necessary not only to select the treatment methods that can promote the proliferation and differentiation of dental pulp stem cells safely and effectively but also to continue to explore the scaffold materials suitable for the structural and functional diversity of the pulp chamber.