Objective To investigate the clinical efficacy of the median and paramedian approaches in ultrasound-guided combined spinal-epidural anesthesia for patients with hemorrhoidectomy and to evaluate the differences between the two techniques.Methods A retrospective analysis was performed on 90 AIDS patients who underwent hemorrhoidectomy under combined spinal-epidural anesthesia from February 2024 to March 2025.The patients were categorized into two groups based on the ultrasound-guided puncture approach:a midline approach group and a paramedian approach group.In the midline approach group,anatomical landmarks such as the spinous process space,facet joint,and transverse process were identified in the transverse axial plane for accurate localiza-tion.The puncture site was determined as the midpoint of the interspinous space along the spinal midline,and an out-of-plane puncture technique was utilized.In the paramedian approach group,positioning was performed by scanning the lamina in the sagittal oblique plane.The dorsal complex structure space with the clearest visibility was centered under the probe,and an in-plane puncture technique was applied,with the needle advanced from the caudal to the cephalad direction to complete the combined spinal-epidural procedure.Data regarding puncture performance,complications,and anesthetic efficacy were systematically recorded and assessed for both groups.Results Intergroup analysis demonstrated that the paramedian approach group exhibited significantly fewer total puncture attempts and a higher success rate on the first attempt(P<0.05).No significant differences were observed between the two groups regarding the need to change puncture levels or modify the puncture approach(P>0.05).The midline approach group showed a significantly higher incidence of paresthesia and a greater frequency of post-operative puncture site tenderness at two days post-surgery compared to the paramedian approach group(P<0.05).Anesthetic efficacy was comparable between the two groups,with no statistically significant difference detected(P>0.05).Conclusions In hemorrhoidectomy procedures for AIDS patients,the paramedian approach demon-strates advantages over the median approach in terms of puncture success and reduced complications associated with ultrasound-guided combined spinal-epidural anesthesia.However,no significant difference in anesthetic efficacy is observed between the two approaches.

Objective To investigate the clinical efficacy of the median and paramedian approaches in ultrasound-guided combined spinal-epidural anesthesia for patients with hemorrhoidectomy and to evaluate the differences between the two techniques.Methods A retrospective analysis was performed on 90 AIDS patients who underwent hemorrhoidectomy under combined spinal-epidural anesthesia from February 2024 to March 2025.The patients were categorized into two groups based on the ultrasound-guided puncture approach:a midline approach group and a paramedian approach group.In the midline approach group,anatomical landmarks such as the spinous process space,facet joint,and transverse process were identified in the transverse axial plane for accurate localiza-tion.The puncture site was determined as the midpoint of the interspinous space along the spinal midline,and an out-of-plane puncture technique was utilized.In the paramedian approach group,positioning was performed by scanning the lamina in the sagittal oblique plane.The dorsal complex structure space with the clearest visibility was centered under the probe,and an in-plane puncture technique was applied,with the needle advanced from the caudal to the cephalad direction to complete the combined spinal-epidural procedure.Data regarding puncture performance,complications,and anesthetic efficacy were systematically recorded and assessed for both groups.Results Intergroup analysis demonstrated that the paramedian approach group exhibited significantly fewer total puncture attempts and a higher success rate on the first attempt(P<0.05).No significant differences were observed between the two groups regarding the need to change puncture levels or modify the puncture approach(P>0.05).The midline approach group showed a significantly higher incidence of paresthesia and a greater frequency of post-operative puncture site tenderness at two days post-surgery compared to the paramedian approach group(P<0.05).Anesthetic efficacy was comparable between the two groups,with no statistically significant difference detected(P>0.05).Conclusions In hemorrhoidectomy procedures for AIDS patients,the paramedian approach demon-strates advantages over the median approach in terms of puncture success and reduced complications associated with ultrasound-guided combined spinal-epidural anesthesia.However,no significant difference in anesthetic efficacy is observed between the two approaches.

Acute respiratory tract infection is the most common infectious disease in children, which seriously threatens children′s health.Rapid and accurate etiological diagnosis is of great significance for the clinical treatment and control of these diseases.Pathogen nucleic acid test was applied and became the main method of respiratory tract infection diagnosis for its high sensitivity and specificity.To regulate the application of pathogen nucleic acid amplification test in respiratory tract infection in children, improve the diagnosis level, expert consensus on nucleic acid amplification test of respiratory pathogens in children was prepared to guide the application and promote pathogens diagnosis ability.

Wnt signaling has emerged as a major regulator of tissue development by governing the self-renewal and maintenance of stem cells in most tissue types. As a key upstream regulator of the Wnt pathway, the transmembrane E3 ligase ZNRF3 has recently been established to play a role in negative regulation of Wnt signaling by targeting Frizzled (FZD) receptor for ubiquitination and degradation. However, the upstream regulation of ZNRF3, in particular the turnover of ZNRF3, is still unclear. Here we report that ZNRF3 is accumulated in the presence of proteasome inhibitor treatment independent of its E3-ubiquitin ligase activity. Furthermore, the Cullin 1-specific SCF complex containing β-TRCP has been identified to directly interact with and ubiquitinate ZNRF3 thereby regulating its protein stability. Similar with the degradation of β-catenin by β-TRCP, ZNRF3 is ubiquitinated by β-TRCP in both CKI-phosphorylation- and degron-dependent manners. Thus, our findings not only identify a novel substrate for β-TRCP oncogenic regulation, but also highlight the dual regulation of Wnt signaling by β-TRCP in a context-dependent manner where β-TRCP negatively regulates Wnt signaling by targeting β-catenin, and positively regulates Wnt signaling by targeting ZNRF3.
Cells, Cultured ; Humans ; Proteolysis ; Ubiquitin-Protein Ligases ; metabolism ; Ubiquitination ; beta-Transducin Repeat-Containing Proteins ; metabolism