Objective:To explore the application value of a novel bag respirator assisted ventilation device in postoperative transport under general anesthesia with laryngeal mask.Methods:A total of 133 patients in postoperative transport who underwent elective bron-choscopy or treatment under general anesthesia with laryngeal mask in the First Affiliated Hospital of Chongqing Medical University,from June to August 2023 were selected.The patients were randomly divided into control group(n=65)and experimental group(n=68),and received manual bag respirator assisted ventilation and the novel bag respirator assisted ventilation device during their postop-erative transport,respectively.The pulse oxygen saturation(SpO2),heart rate(HR),and ventilation frequency during transport,trans-port duration,and transport-related adverse events were compared between the two groups.Results:The difference in SpO2 was signifi-cant when comparing the two groups(Fbetween groups=18.588,P<0.001),and the SpO2 of patients in the experimental group was signifi-cantly higher than that of patients in the control group during and after transport(P<0.001).The difference in HR was not significant when comparing the two groups(Fbetween groups=0.089,P=0.766),but it was significant between the control and experimental groups before and after transport(Ftime point=12.430,P<0.001);the HR in the con-trol and experimental groups before and during transport was signifi-cantly lower than that after transport(all P<0.001).The ventilation frequency of the experimental group was significantly lower than that of the control group(P<0.001).The transport duration in the ex-perimental group was longer than that in the control group,but the difference was not significant(P=0.987).Both groups successfully completed the trial without transport-related adverse events and achieved safe transport.Conclusion:Compared with the manual bag respirator assisted ventilation technology,the novel bag respirator assisted ventilation device for respiratory support during postopera-tive transport in patients under general anesthesia with laryngeal mask is more able to reduce the impact on the patient's hemodynam-ics and conducive to the maintenance of the patient's stable vital signs,showing a good clinical application value.It is expected to be a safe and effective ventilation method during intrahospital transport in some patients under general anesthesia.

The cytotoxic effect targeting hepatitis B virus (HBV) infected hepatocytes from virus-specific cytotoxic T cells and the neutralizing antibodies secreted by virus-specific B cells play an important role in the immune control and elimination of HBV. In patients with chronic hepatitis B, the liver immune microenvironment usually presents a suppression state, and virus-specific immune cells are mostly exhausted. Studies on the interaction between HBV and host immunity during infection, especially the influence of various viral proteins on immune cell function, will contribute to understanding the mechanism of the chronicity of HBV infection, disease progression, and optimization of immunotherapy against HBV. The review summarized the suppressive effects of HBV viral proteins on the host innate immunity and adaptive immune system, to help us understanding the mechanism(s) relevant to the observation that a CHB patient with HBeAg loss and lower HBsAg level is more likley achieving functionall cure. and expect to provide new sights for accelerate virus clearance and achieve functional cure of chronic hepatitis B, by removing the HBV viral proteins and consequently, liberting host immune from suppression state.

Objective To investigate the ability and underlying mechanism of hepatitis B virus X protein (HBx)regulationofPolo-likekinase 1 (Plk1)expression.Methods The human HCC cell line HepG2 was transfected (transiently and stably) with an HBx plasmid expression vector (pCMV-HA-HBx) or empty plasmid vector (control),with and without expression plasmids with the Plk1 promoter.Effects on Plk1 expression were assessed by western blotting.Functional effects on the Plk1 promoter were assessed by luciferase reporter assay.Effects on the mRNA level of Plk1 in S phase HepG2 cells were assessed by quantitative real-time reverse transcriptase polymerase chain reaction.After blocking protein synthesis by treatment with cycloheximide (CHX),the turnover rate of Plk1 was assessed by western blotting.Lastly,the effect of HBx on cell cycle was assessed by flow cytometry.Results HBx did not increase the protein expression of Plk1 in non-synchronized HepG2 cells,but did significantly up-regulate the Plkt protein level in the synchronized S phase cells (P =0.026 and P =0.003,respectively).Ectopic expression of HBx did not increase the mRNA level of Plk1 in HepG2 cells,but did inhibit the degradation of Plk1,as evidenced by an increased half-life of Plk1 protein (from 30 to 90 minutes).The HBx-expressing HepG2 cells showed more trequent entry into the S or G2/M phase than the control cells (31.65％ vs.24.56％ or 9.43％ vs.4.47％,respectively) and less in the G0/G1 phase (decrease from 70.97％ to 58.92％ for the HBx-expressing HepG2 cells).Conclusion HBx is able to up-regulate the expression of Plk1 in HepG2 cells by a mechanism involving stabilization of the Plkl protein primarily in the S phase of the cell cycl.

Elucidation of molecular mechanisms underlying host-pathogen interactions is important for control and treatment of infectious diseases worldwide. Within the last decade, mass spectrometry (MS)-based proteomics has become a powerful and effective approach to better understand complex and dynamic host-pathogen interactions at the protein level. Herein we will review the recent progress in proteomic analyses towards bacterial infection of their mammalian host with a particular focus on enteric pathogens. Large-scale studies of dynamic proteomic alterations during infection will be discussed from the perspective of both pathogenic bacteria and host cells.
Animals ; Bacteria ; chemistry ; pathogenicity ; Bacterial Infections ; microbiology ; pathology ; Bacterial Proteins ; isolation & purification ; metabolism ; Host-Pathogen Interactions ; Humans ; Mass Spectrometry ; Protein Processing, Post-Translational ; Proteomics