Objective To investigate the effects of microRNA-602(miR-602)on the growth and invasion of SH-SY5Y cell lines in neuroblastoma(NB)and its possible mechanism.Methods Real-time quantitative polymerase chain reaction(qPCR)was used to detect the expression of miR-602 in SH-SY5Y cell lines of NB and HEK-293 cells of normal human embryonic kidney.MTT asssy was used to detect the effect of miR-602 on the growth of SH-SY5Y cell lines.Transwell invasion assay was used to detect the effect of miR-602 on the invasion of SH-SY5Y cell lines of NB.The possible target genes of miR-602 were screened,and the regulatory effects of miR-602 on target genes were verified by the luciferase reporter gene system combined with qPCR technology.Results The expression level of HEK-293 cells in healthy people embryonic kidney was normal-ized as 1,the relative expression level of miR-602 in SH-SY5Y cell lines of NB was 3.83±0.85 and the differ-ence was statistically significant(P＜0.01).The results of MTT assay showed that the cell relative activity of miR-602 mimics group(1.20±0.05)was higher than that of NC mimics group(1.00±0.01),while the cell relative activity of miR-602 inhibitor group(0.76±0.04)was lower than that of NC inhibitor group(1.00±0.01),and the differences were statistically significant(P＜0.05).The results of Transwell invasion experi-ment showed that the number of transmembrane cells in miR-602 mimics group(193.33±8.02)was higher than that in NC mimics group(97.33±20.03).The number of transmembrane cells of miR-602 inhibitor group(62.01±11.79)was lower than that of NC inhibitor group(132.33±11.24),and the differences were statistically significant(P＜0.05).qPCR results showed that the expression level of recombinant human Sprouty-related EVH1 domain(SPRED1)mRNA in miR-602 mimics group was 0.56±0.08 compared with the control NC mimics group.The relative change factor of SPRED1 mRNA expression level in miR-602 in-hibitor group was 4.16±0.91 compared with NC inhibitor group,and the differences were statistically signif-icant(P＜0.01).Conclusion miR-602 is highly expressed in SH-SY5Y cell lines,and it may promote the growth and invasion in SH-SY5Y cells by targeting the regulation of expression of SPRED1.

Diabetic nephropathy(DN)is the leading cause of end-stage renal disease.Renal tubulointerstitial injury is an important pathophysiological basis that contributes to the progression of DN to end-stage renal disease.Stress-induced senescence of renal tubular epithelial cells(RTECs)forms a key link that causes tubulointerstitial injury.In recent years,it has been reported that organelles,such as endoplasmic reticulum,mitochondria,and lysosomes,in RTECs are damaged to varying degrees in DN,and that their functional imbalance may lead to stress-induced senescence of RTECs,thereby causing sustained cellular and tissue-organ damage,which in turn promotes the progression of the disease.However,the core mechanism underlying changes in the senescence microenvironment caused by stress-induced senescence of RTECs in DN is still not understood.In addition,the mechanism by which organelles lose homeostasis also needs to be further investigated.Herein,we described the specific pathophysiological mechanisms of renal tubular injury,stress-induced senescence of RTECs,and their association with organelles in the context of DN in order to provide reference for the next-step research,as well as the development of new therapeutic strategies.

Objective:To evaluate the clinical efficacy of multi-disciplinary single center's CCCG-HB-2016 regimen in the treatment of hepatoblastoma (HB) in children.Methods:Clinical data of 36 HB patients treated with CCCG-HB-2016 program from Aug 2016 to March 2020 were analyzed.Results:These 36 patients included 20 boys and 16 girls. The serum AFP was all higher than 2 792 ng/ml,there was a correlation between AFP and tumor risk stratification ( H=14.973, P<0.05). Twenty eight cases (77.78%) were epithelial type and 8 cases (22.22%) were mixed epithelial mesenchymal type.All children were treated by tumor resection combined with chemotherapy, and there was a correlation between tumor risk stratification and surgical resection of liver lobe ( H=8.847, P<0.05). The probability of bone marrow suppression in the low-risk group was 58.33% (35/60),that in the intermediate-risk group was 73.49% (61/83) and in the high-risk group was 80.23% (69/86).All 36 cases were followed up to March 31, 2020,with an average follow-up of 21.9 months and the median survival was 22.5 months.The overall survival rate (OS) and event-free survival rate (EFS) were 97.2% and 83.3% respectively. Conclusions:The multidisciplinary CCCG-HB-2016 regimen was with a high success rate and along with a high incidence of bone marrow suppression.

Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) has become one major threat to human population health. The RNA-dependent RNA polymerase (RdRp) presents an ideal target of antivirals, whereas nucleoside analogs inhibitor is hindered by the proofreading activity of coronavirus. Herein, we report that corilagin (RAI-S-37) as a non-nucleoside inhibitor of SARS-CoV-2 RdRp, binds directly to RdRp, effectively inhibits the polymerase activity in both cell-free and cell-based assays, fully resists the proofreading activity and potently inhibits SARS-CoV-2 infection with a low 50% effective concentration (EC