Objective:To investigate the changes and clinical significance of serum matrix metalloproteinase-9 (MMP-9), squamous cell carcinoma antigen (SCC), cytokeratin 19 fragment (CYFRA21-1), carcinoembryonic antigen (CEA) and neuron-specific enolase (NSE) in peripheral lung cancer.Methods:Sixty-eight patients with peripheral lung cancer who received treatment in Luqiao Hospital of Taizhou Enze Medical Center (Group) between January 2017 and January 2020 were included in the observation group. Sixty-five patients with benign lung diseases who concurrently received treatment in the same hospital were included in the observation group 1, and another 65 healthy participants who concurrently received physical examination were included in the control group. Serum levels of MMP-9, CYFRA21-1, SCC, NSE and CEA were compared among the three groups. The sensitivity and specificity of using these indicators alone and in combination in the diagnosis of peripheral lung cancer were compared.Results:Serum levels of MMP-9, CYFRA21-1, SCC, NSE and CEA in the observation group (14.98 ± 2.10) ng/mL, (17.13 ± 2.71) ng/mL, (1.98 ± 0.41) μg/mL, (24.13 ± 2.10) ng/mL and (17.10 ± 2.10) ng/mL, respectively, which were significantly higher than those in the observation group 1 [(9.12 ± 1.41) ng/mL, (10.12 ± 1.58) ng/mL, (1.37 ± 0.31) μg/mL, (16.31 ± 1.78) ng/mL, (12.13 ± 1.79) ng/mL] and control group [(5.10 1 ± 0.68) ng/mL, (6.02 ± 0.94) ng/mL, (0.71 ± 0.11) μg/mL, (11.10 ± 1.02) ng/mL, (8.13 ± 1.02) ng/mL] ( F1 = 932.781, F2 = 737.100, F3 = 368.591, F4 = 989.851, F5 = 462.291, all P < 0.05). Serum levels of MMP-9, CYFRA21-1, SCC, NSE and CEA in patients with stage I-II peripheral lung cancer were (11.12 ± 2.10) ng/mL, (9.12 ± 1.85) ng/mL, (1.52 ± 0.21) μg/mL, (18.12 ± 3.02) ng/mL, (7.52 ± 1.02) ng/mL, respectively, which were significantly lower than those in patients with stage III-IV peripheral lung cancer [(15. 89 ± 2.18) ng/mL, (21.56 ± 2.11) ng/mL, (2.04 ± 0.31) μg/mL, (28.15 ± 2.62) ng/mL, (15.12 ± 1.55) ng/mL, t1 = 9.013, t2 = 25.146, t3 = 7.714, t4 = 14.586, t5 = 22.705, all P < 0.05]. The sensitivity (83.33%) and specificity (86.67%) of combined detection of all indicators were significantly higher than those of single detection of MMP-9 (50.00%, 59.68%), CEA (50.00%, 61.29%), CYFRA21-1 (66.67%, 58.06%), SCC (50.00%, 54.84%) or NSE (66.67%, 58.06%) (all P < 0.05). Conclusion:Serum levels of MMP-9, CYFRA21-1, SCC, NSE and CEA in patients with peripheral lung cancer are significantly increased, which has an important value in the diagnosis of peripheral lung cancer. The combined detection of the above indicators can increase the diagnostic accuracy of peripheral lung cancer in the clinic.

Objective:To explore the cumulative delivery rate and related factors of infertile women with anti-Müllerian hormone (AMH) no more than 1 μg/L during in vitro fertilization and embryo transfer (IVF-ET) treatment. Methods:In this retrospective cohort study, we analyzed 793 infertile women with AMH no more than 1 μg/L and underwent their first IVF-ET treatment in Henan Provincial People's Hospital between July 2016 and February 2018. All the patients were categorized into two groups according to cumulative delivery with at least one live baby or not. Baseline data and outcomes were compared between the two groups. The regression analysis was conducted to identify the independent factors of cumulative delivery rate and clinical outcomes of different age groups were compared.Results:1) The total cumulative delivery rate per start cycle was 23.83% (189/793). There were significant differences in age [33(30,37) years vs. 39(34,43) years], AMH [0.67(0.46,0.87) μg/L vs. 0.51(0.22,0.74) μg/L], antral follicle count (AFC) [6(4,8) vs. 4(2,6)], basal follicle-stimulating hormone (bFSH) [9.11(6.98,11.03) IU/L vs. 11.06(7.75,13.13) IU/L], starting dosage of gonadotropin (Gn) used [225.0(187.5,225.0) IU vs. 225.0(225.0,300.0) IU], total dosage of Gn used [2 475.0(1 800.0,3 137.5) IU vs. 2 100.0(1 575.0,2 850.0) IU], duration of Gn stimulation [10(8,12) d vs. 9(6,11) d], number of oocytes retrieved [5.0(3.0,7.5) vs. 2.5(1.0,4.0)], number of M II oocytes [4(3,6) vs. 2(1,3)] and number of available cleavage embryos [3.0(2.0,4.5) vs.1.0(0,2.0)] between the two groups (all P<0.001). 2) Multivariate logistic regression analysis suggested that age ( OR=0.878, 95% CI=0.846-0.911, P<0.001) was the vital factor of cumulative delivery rate. The cumulative pregnancy rate and the cumulative delivery rate were significantly decreased with age increasing (all P<0.001). And the cancelation rate of group with age more than 40 years was much higher ( P<0.001). Conclusion:Age was the independent influencing factor of cumulative delivery rate. The cumulative delivery rate decreased significantly with age increasing.

Objective:To explore the cumulative delivery rate and related factors of infertile women with anti-Müllerian hormone (AMH) no more than 1 μg/L during in vitro fertilization and embryo transfer (IVF-ET) treatment. Methods:In this retrospective cohort study, we analyzed 793 infertile women with AMH no more than 1 μg/L and underwent their first IVF-ET treatment in Henan Provincial People's Hospital between July 2016 and February 2018. All the patients were categorized into two groups according to cumulative delivery with at least one live baby or not. Baseline data and outcomes were compared between the two groups. The regression analysis was conducted to identify the independent factors of cumulative delivery rate and clinical outcomes of different age groups were compared.Results:1) The total cumulative delivery rate per start cycle was 23.83% (189/793). There were significant differences in age [33(30,37) years vs. 39(34,43) years], AMH [0.67(0.46,0.87) μg/L vs. 0.51(0.22,0.74) μg/L], antral follicle count (AFC) [6(4,8) vs. 4(2,6)], basal follicle-stimulating hormone (bFSH) [9.11(6.98,11.03) IU/L vs. 11.06(7.75,13.13) IU/L], starting dosage of gonadotropin (Gn) used [225.0(187.5,225.0) IU vs. 225.0(225.0,300.0) IU], total dosage of Gn used [2 475.0(1 800.0,3 137.5) IU vs. 2 100.0(1 575.0,2 850.0) IU], duration of Gn stimulation [10(8,12) d vs. 9(6,11) d], number of oocytes retrieved [5.0(3.0,7.5) vs. 2.5(1.0,4.0)], number of M II oocytes [4(3,6) vs. 2(1,3)] and number of available cleavage embryos [3.0(2.0,4.5) vs.1.0(0,2.0)] between the two groups (all P<0.001). 2) Multivariate logistic regression analysis suggested that age ( OR=0.878, 95% CI=0.846-0.911, P<0.001) was the vital factor of cumulative delivery rate. The cumulative pregnancy rate and the cumulative delivery rate were significantly decreased with age increasing (all P<0.001). And the cancelation rate of group with age more than 40 years was much higher ( P<0.001). Conclusion:Age was the independent influencing factor of cumulative delivery rate. The cumulative delivery rate decreased significantly with age increasing.

AIM: To study the expression and distribution of osteopontin (OPN) in lungs and pulmonary arteries in pulmonary hypertensive rats induced by hypoxia-hypercapnia, and to explore the role of OPN in pathogenesis of pulmonary hypertension. METHODS: Forty-eight male Sprague-Dawley rats (Weight 180 g-220 g) were randomly divided into four groups: normal control group (NC), hypoxic hypercapnia 1-week,2-week and 4-week group (1HH, 2HH and 4HH). The expressions of OPN mRNA and protein in lungs and pulmonary arteries were detected by RT-PCR and immunohistochemistry. ELISA was used to detect the concentration of OPN in lung homogenates. The content of OPN in pulmonary arteries was detected by Western blotting. RESULTS: ① The mean pulmonary artery pressure (mPAP) and weight ratio of right ventricle to left ventricle and septum [RV/(LV+S)] in all hypoxic hypercapniac groups were higher than those in normal control group (P0.05). ② The expression of OPN mRNA was significantly increased in pulmonary arteries and lung tissues in hypoxic hypercapnic groups compared with normal control group (P