OBJECTIVE To analyze the incidence of infectious diseases in a certain department from 2015 to 2022,to provide scientific basis for formulating health and epidemic prevention measures.METHODS Descriptive epide-miological methods were used to analyze the incidence of infectious diseases.RESULTS Over the eight-year period,a total of 3,635 cases of 35 types of infectious diseases were reported.The top five diseases in terms of incidence were chickenpox,influenza,tuberculosis,viral hepatitis and acute febrile respiratory diseases.From the perspec-tive of transmission routes,respiratory infectious diseases were the most common,followed by blood-borne and sexually transmitted diseases,and gastrointestinal infectious diseases.Regarding time distribution,the high-est number of cases occurred in the first quarter,with the least in the third quarter.From the perspective of popu-lation distribution,soldiers have the highest incidence of the disease,followed by military officers,with age of on-set ranging from 17 to 25 years old.CONCLUSIONS Respiratory infectious diseases are the primary cause of the epidemic,with an obvious seasonal distribution characteristics,and sexually transmitted diseases show a slow growth trend.It is imperative to strengthen monitoring,early warning and timely disposal of respiratory infectious diseases;meanwhile health education and intervention for risk factors related to sexually transmitted diseases should be strengthened.

OBJECTIVE To analyze the incidence of infectious diseases in a certain department from 2015 to 2022,to provide scientific basis for formulating health and epidemic prevention measures.METHODS Descriptive epide-miological methods were used to analyze the incidence of infectious diseases.RESULTS Over the eight-year period,a total of 3,635 cases of 35 types of infectious diseases were reported.The top five diseases in terms of incidence were chickenpox,influenza,tuberculosis,viral hepatitis and acute febrile respiratory diseases.From the perspec-tive of transmission routes,respiratory infectious diseases were the most common,followed by blood-borne and sexually transmitted diseases,and gastrointestinal infectious diseases.Regarding time distribution,the high-est number of cases occurred in the first quarter,with the least in the third quarter.From the perspective of popu-lation distribution,soldiers have the highest incidence of the disease,followed by military officers,with age of on-set ranging from 17 to 25 years old.CONCLUSIONS Respiratory infectious diseases are the primary cause of the epidemic,with an obvious seasonal distribution characteristics,and sexually transmitted diseases show a slow growth trend.It is imperative to strengthen monitoring,early warning and timely disposal of respiratory infectious diseases;meanwhile health education and intervention for risk factors related to sexually transmitted diseases should be strengthened.

Objective:To explore the potential molecular mechanism of umbilical cord-derived mesenchymal stem cells (UCMSCs) to improve ovarian function in premature ovarian failure (POF) rabbits.Methods:Ten rabbits were randomly divided into 2 groups (five rabbits in each), treatment group and model group. A rabbit model of POF was established using an intraperitoneal injection of cyclophosphamide for 2 d (50 mg·kg -1·d -1). After a week, the treatment group received UCMSCs intravenously via a marginal ear vein and model group received equal amounts of sterile water. Ovarian samples were collected on day 28 and ovaries morphology was observed by hematoxylin-eosin staining. Tissue inhibitor of metalloproteinases 1 (TIMP1), fibronectin (FN) and matrix metalloproteinases 9 (MMP9) protein and mRNA expression were detected by Western blotting, PCR, and immunohistochemistry. Results:1) In model group, ovaries of the ovarian tissue displayed as reduced number of primordial follicles, along with increased number of atretic follicles. The granular layer cells of every follicle grade were arranged in disorder. The oocyte nucleus appeared pyknotic and apoptotic changed. Fibrosis was performed in the ovarian stroma. While in treatment group, the number of primordial and primary follicles in the ovarian tissue was large, and there were few atretic follicles. The granule cells of every follicle grade were arranged regularly. 2) Compared with model group, the expressions of TIMP1 protein (0.546±0.021 vs. 0.820±0.085) and FN protein (0.221±0.065 vs. 0.516±0.064) in treatment group were significantly increased (all P<0.001), while the expression of MMP9 protein (0.504±0.049 vs. 0.204±0.066) in treatment group was significantly decreased ( P<0.001). 3) Compared with model group, the expressions of TIMP1 mRNA (0.217±0.024 vs. 0.470±0.039) and FN mRNA (0.039±0.006 vs. 0.125±0.012) in treatment group were significantly increased (all P<0.001), while the expression of MMP9 mRNA (0.009±0.000 vs. 0.002±0.000) in treatment group was significantly decreased ( P<0.001). 4) TIMP1 and FN were mainly expressed in the granule cells and egg cell membranes. TIMP1 was also expressed in ovarian interstitial tissue, and the expression levels were significantly increased in treatment group compared with model group ( P<0.001, P=0.005). MMP9 was mainly expressed in granule cells and ovarian interstitial tissue, and the expression levels were significantly decreased in treatment group compared with model group ( P<0.001). Conclusion:UCMSCs were able to improve ovarian function by regulating the levels of TIMP1, FN and MMP9 in ovarian tissue.

Objective:To explore the potential molecular mechanism of umbilical cord-derived mesenchymal stem cells (UCMSCs) to improve ovarian function in premature ovarian failure (POF) rabbits.Methods:Ten rabbits were randomly divided into 2 groups (five rabbits in each), treatment group and model group. A rabbit model of POF was established using an intraperitoneal injection of cyclophosphamide for 2 d (50 mg·kg -1·d -1). After a week, the treatment group received UCMSCs intravenously via a marginal ear vein and model group received equal amounts of sterile water. Ovarian samples were collected on day 28 and ovaries morphology was observed by hematoxylin-eosin staining. Tissue inhibitor of metalloproteinases 1 (TIMP1), fibronectin (FN) and matrix metalloproteinases 9 (MMP9) protein and mRNA expression were detected by Western blotting, PCR, and immunohistochemistry. Results:1) In model group, ovaries of the ovarian tissue displayed as reduced number of primordial follicles, along with increased number of atretic follicles. The granular layer cells of every follicle grade were arranged in disorder. The oocyte nucleus appeared pyknotic and apoptotic changed. Fibrosis was performed in the ovarian stroma. While in treatment group, the number of primordial and primary follicles in the ovarian tissue was large, and there were few atretic follicles. The granule cells of every follicle grade were arranged regularly. 2) Compared with model group, the expressions of TIMP1 protein (0.546±0.021 vs. 0.820±0.085) and FN protein (0.221±0.065 vs. 0.516±0.064) in treatment group were significantly increased (all P<0.001), while the expression of MMP9 protein (0.504±0.049 vs. 0.204±0.066) in treatment group was significantly decreased ( P<0.001). 3) Compared with model group, the expressions of TIMP1 mRNA (0.217±0.024 vs. 0.470±0.039) and FN mRNA (0.039±0.006 vs. 0.125±0.012) in treatment group were significantly increased (all P<0.001), while the expression of MMP9 mRNA (0.009±0.000 vs. 0.002±0.000) in treatment group was significantly decreased ( P<0.001). 4) TIMP1 and FN were mainly expressed in the granule cells and egg cell membranes. TIMP1 was also expressed in ovarian interstitial tissue, and the expression levels were significantly increased in treatment group compared with model group ( P<0.001, P=0.005). MMP9 was mainly expressed in granule cells and ovarian interstitial tissue, and the expression levels were significantly decreased in treatment group compared with model group ( P<0.001). Conclusion:UCMSCs were able to improve ovarian function by regulating the levels of TIMP1, FN and MMP9 in ovarian tissue.

Objective To investigate the metabolism and function of the intestinal microbiota from liver cirrhosis patients.Methods Sixteen cases of liver cirrhosis and twenty normal individuals were selected , whose intestinal microbiota metagenomic DNA was extracted , followed by high-throughput Solexa sequencing and the bioinformatics analysis of metabo-lism and function annotation to compare the differences between the patients and normal subjects and find out about the cir -rhosis-related functions .Results The functional diversity was significantly reduced in the intestinal microbiota of cirrhotic patients.At the module or pathway level , the intestinal microbiota of patients showed an enrichment in metabolisms of drugs, essential amino acid , propanoate metabolism and inflammatory reaction , whereas an opposite tendency was observed in the metabolic ability of butyrate , bile acid and cell cycle .Conclusion Under the influence of liver cirrhosis , the growth environment in the intestine is destroyed , causing, the intestinal microbiota the exhibit some compensation to adapt to the changed intestinal micro-environment .