Objective To investigate the effect of recombinant human growth hormone (rhGH) on rat liver injury with severe acute pancreatitis.Methods Severe acute pancreatitis model was established by injection of sodium taurocholate into the pancreatobiliary duct in 40 male Sprague-Dawley rats.Rats were randomly divided into experiment group (n =20) and control group (n =20).Another 20 male SD rats injected saline served as negative control group.The experiment group were treated with subcutaneously injected rhGH for 3 days,1 U·kg-1 ·d-1.12 h and 24 h after operation,the level of ALT,AST,TNF-α,IL-1 β,SOD,MDA,endotoxin and D-lactate was detected respectively;the degree of live cell apoptosis and pathological score of pancreatic tissue were compared among these groups.Results In comparison with negative control group,the level of ALT,AST,TNF-α,IL-1β,MDA,endotoxin and D-lactate,the liver cell apoptosis index and pathological score of pancreatic tissuc were significantly higher in the control group and experiment group at 12 h and 24 h after operation (P ＜ 0.05).ALT,AST,TNF-α,endotoxin and D-lactate at 12 h and 24 h were significantly lower in the experiment group (P ＜ 0.05).MDA level significantly declined at 12 h and 24 h after operation in experiment group(P ＜ 0.05).Liver cell apoptosis index of the control group was higher than experiment group (P =0.003).Conclusion rhGH pretreatment relieves liver injury in rat with severe acute pancreatitis.

Objective To observe the effect of cabin pressure change on gallstone formation by using hypobaric chamber to simulate hypobaric circumstance of civil aircraft in flight.Methods Forty-eight rabbits were randomly divided into control group (n＝ 12) that was with fodder,test group A (n ＝ 12) that was with 0.8％ cholesterol fodder,test group B (n ＝ 12) that was with standard fodder and hypobaric exposures 2 h a day for 28 days,and test group C (n＝12) that was with 0.8% cholesterol fodder and hypobaric exposures once a day for 28 days.The rate of gallstone formation was calculated for each group respectively.Quantitative analyses of 99 mTc-EHIDA hepatobiiiary dynamic image,the bile viscosity and bile ingredient were made for each group.Results ①The rate of gallstone formation was 0.0% in control group,50.0% in test group A (6/12),8.3％ in test group B (1/12) and 66.7％ in test group C (8/12).(②)Compared with control group,test group A,B and C showed significant delay in duodenal appearance time (DAT) (t＝4.779-17.093,P＜0.01).DAT of test group C was longer than that of test group A and test group B respectively (t＝2.693,5.654,P＜0.05 or P＜0.01).③Compared with control group,test group A and C showed higher high shear rate and low shear rate (t＝2.063 2.343,P＜0.05),and test group C had higher medium shear rate (t＝2.111,P＜0.05).(④) Compared with control group and test group B,test group A and test group C had higher level of bile acid (t 2.001 2.017,P＜0.05) and higher level of cholesterol (t＝ 2.445 2.762,P＜0.05).The level of phospholipid of test group C was lower than that of control group and test group B (t＝2.447,2.320,P＜0.05).Conclusions High cholesterol intake and barometric pressure change in flight would facilitate the gallstone formation in rabbits to some extent.

Objective To observe the effect of cabin pressure change on gallstone formation by using hypobaric chamber to simulate hypobaric circumstance of civil aircraft in flight.Methods Forty-eight rabbits were randomly divided into control group (n＝ 12) that was with fodder,test group A (n ＝ 12) that was with 0.8％ cholesterol fodder,test group B (n ＝ 12) that was with standard fodder and hypobaric exposures 2 h a day for 28 days,and test group C (n＝12) that was with 0.8% cholesterol fodder and hypobaric exposures once a day for 28 days.The rate of gallstone formation was calculated for each group respectively.Quantitative analyses of 99 mTc-EHIDA hepatobiiiary dynamic image,the bile viscosity and bile ingredient were made for each group.Results ①The rate of gallstone formation was 0.0% in control group,50.0% in test group A (6/12),8.3％ in test group B (1/12) and 66.7％ in test group C (8/12).(②)Compared with control group,test group A,B and C showed significant delay in duodenal appearance time (DAT) (t＝4.779-17.093,P＜0.01).DAT of test group C was longer than that of test group A and test group B respectively (t＝2.693,5.654,P＜0.05 or P＜0.01).③Compared with control group,test group A and C showed higher high shear rate and low shear rate (t＝2.063 2.343,P＜0.05),and test group C had higher medium shear rate (t＝2.111,P＜0.05).(④) Compared with control group and test group B,test group A and test group C had higher level of bile acid (t 2.001 2.017,P＜0.05) and higher level of cholesterol (t＝ 2.445 2.762,P＜0.05).The level of phospholipid of test group C was lower than that of control group and test group B (t＝2.447,2.320,P＜0.05).Conclusions High cholesterol intake and barometric pressure change in flight would facilitate the gallstone formation in rabbits to some extent.