Objective:To investigate the association between cardiometabolic multimorbidity(CMM)and cardiometabolic diseases(CMDs)with frailty in elderly patients suffering from chronic diseases in rural Shandong.Additionally, it seeks to analyze the role of self-efficacy in chronic disease management in mitigating the risk of frailty associated with CMM and CMDs.Methods:A multi-stage cluster random sampling method was employed to select 2, 778 elderly patients aged 60 and above with chronic diseases in rural Shandong Province.Frailty was assessed using the Frailty Phenotype(FP), while self-efficacy in chronic disease management was measured using the Self-Efficacy to Manage Chronic Disease Scale(SEMCD).For the analysis, binary logistic regression and marginal effects were utilized.Results:The prevalence of frailty among elderly patients with chronic diseases in rural Shandong was found to be 12.28%, while the prevalence of CMM was reported at 53.20%.Binary logistic regression analysis revealed that frailty was significantly associated with several factors, including age, education level, annual per capita income, employment status, and self-efficacy in managing chronic disease(all P<0.05).Notably, increases in CMM( P=0.001)and the number of CMDs( P<0.001)were linked to a heightened risk of frailty.Furthermore, marginal effects analysis indicated that individuals in the high self-efficacy group exhibited significantly lower risks of frailty associated with CMM and CMDs compared to those in the low self-efficacy group, with risk reductions of 96.9%(95% CI: -1.258--0.681, P<0.001)and 85.7%(95% CI: -1.175--0.539, P<0.001), respectively. Conclusions:Both the presence of CMM and a higher number of CMDs are linked to an increased risk of frailty.However, elevated levels of self-efficacy in chronic disease management can significantly mitigate the frailty risk associated with CMM and CMDs.It is advisable to enhance health education and self-management training, as well as to establish a comprehensive, multidisciplinary, and multidimensional health management system for the elderly.This approach aims to improve self-efficacy in chronic disease management among rural elderly populations, thereby preventing and addressing the onset of frailty.

Objective:To investigate the association between cardiometabolic multimorbidity(CMM)and cardiometabolic diseases(CMDs)with frailty in elderly patients suffering from chronic diseases in rural Shandong.Additionally, it seeks to analyze the role of self-efficacy in chronic disease management in mitigating the risk of frailty associated with CMM and CMDs.Methods:A multi-stage cluster random sampling method was employed to select 2, 778 elderly patients aged 60 and above with chronic diseases in rural Shandong Province.Frailty was assessed using the Frailty Phenotype(FP), while self-efficacy in chronic disease management was measured using the Self-Efficacy to Manage Chronic Disease Scale(SEMCD).For the analysis, binary logistic regression and marginal effects were utilized.Results:The prevalence of frailty among elderly patients with chronic diseases in rural Shandong was found to be 12.28%, while the prevalence of CMM was reported at 53.20%.Binary logistic regression analysis revealed that frailty was significantly associated with several factors, including age, education level, annual per capita income, employment status, and self-efficacy in managing chronic disease(all P<0.05).Notably, increases in CMM( P=0.001)and the number of CMDs( P<0.001)were linked to a heightened risk of frailty.Furthermore, marginal effects analysis indicated that individuals in the high self-efficacy group exhibited significantly lower risks of frailty associated with CMM and CMDs compared to those in the low self-efficacy group, with risk reductions of 96.9%(95% CI: -1.258--0.681, P<0.001)and 85.7%(95% CI: -1.175--0.539, P<0.001), respectively. Conclusions:Both the presence of CMM and a higher number of CMDs are linked to an increased risk of frailty.However, elevated levels of self-efficacy in chronic disease management can significantly mitigate the frailty risk associated with CMM and CMDs.It is advisable to enhance health education and self-management training, as well as to establish a comprehensive, multidisciplinary, and multidimensional health management system for the elderly.This approach aims to improve self-efficacy in chronic disease management among rural elderly populations, thereby preventing and addressing the onset of frailty.

Metabolic reprogramming, a newly recognized trait of tumor biology, is an intensively studied prospect for oncology medicines. For numerous tumors and cancer cell subpopulations, oxidative phosphorylation (OXPHOS) is essential for their biosynthetic and bioenergetic functions. Cancer cells with mutations in isocitrate dehydrogenase 1 (IDH1) exhibit differentiation arrest, epigenetic and transcriptional reprogramming, and sensitivity to mitochondrial OXPHOS inhibitors. In this study, we report that berberine, which is widely used in China to treat intestinal infections, acted solely at the mitochondrial electron transport chain (ETC) complex I, and that its association with IDH1 mutant inhibitor (IDH1^mi) AG-120 decreased mitochondrial activity and enhanced antileukemic effect in vitro andin vivo. Our study gives a scientific rationale for the therapy of IDH1 mutant acute myeloid leukemia (AML) patients using combinatory mitochondrial targeted medicines, particularly those who are resistant to or relapsing from IDH1^mi.
Humans ; Oxidative Phosphorylation ; Berberine ; Electron Transport ; Mitochondria ; Leukemia, Myeloid, Acute ; Isocitrate Dehydrogenase

Objective:To understand the epidemiological characteristics of mpox epidemic in Guangzhou and provide scientific evidence for the prevention and control of the disease.Methods:Based on the mpox surveillance system in Guangzhou, suspected mpox cases with fever and rash were reported by local hospitals at all levels to centers for disease control and prevention in Guangzhou for sampling, investigation and diagnosis. Descriptive epidemiological analysis was conducted on the clinical characteristics and treatment of the mpox cases and positive detection rate reported in Guangzhou as of 24:00 on June 23. Whole genome sequencing of the virus isolates was performed using Illumina Miniseq high-throughput sequencing platform.Results:The first mpox case in Guangzhou was reported on June 10 in 2023. As of 24:00 on June 23, a total of 25 confirmed mpox cases were reported. All the mpox cases were men with a M( Q1, Q3) of 32 (26, 36) years, the majority of the cases were MSM (96.0%). The main clinical features were rash (100.0%, 25/25), lymphadenectasis (100.0%, 25/25) and fever (52.0%, 13/25). Rash usually occurred near the genitals (88.0%, 22/25). The close contacts, mainly family members (40.4%, 23/57), showed no similar symptoms, such as fever or rash. The positive rate of mpox virus in household environment samples was 30.5%. The analyses on 3 complete gene sequences of mpox virus indicated that the strains belonged to West African type Ⅱb clade, B.1.3 lineage. Conclusions:Hidden transmission of mpox virus had occurred in MSM in Guangzhou. However, the size of affected population is relatively limited, and the possibility of wide spread of the virus is low.

Objective To investigate the current situation of contact lens cases′contamination and contact lens hygiene compliance in Wenzhou, so as to provide a basis for further improvement of hygiene for wearers. Methods The general data of the wearer and the present condition of the lens cases hygiene compliance were investigated by the questionnaire, and the contamination was judged by themicrobiological culture. Results The positive rate of bacterial culture in the contact lens cases was 43.96％(40/91). Compared with the positive culture group, the negative group performed better in hand hygiene (χ2=17.624, P<0.01), and the frequency of the case sterilization in the negative group (χ2=43.754, P<0.01), cleaning frequency (χ2=69.737, P<0.01) were higher than the positive group. In the choice of cleaning methods, the proportion of using brushing (χ2=65.441, P<0.01) and re-rinsing (χ2=59.663, P<0.01) in the negative group was higher than that of the positive group. The results were all statistically different.Conclusion The present condition of hygiene compliance of the orthokeratology lens wearers in Wenzhou is not so well, and it needs to be further improved. And agents, scrubbing, rinsing and drying are effective for reducing microbes.

Objective To investigate the regular spot check implementation of central sterile supply department(CSSD) at hospitals of all grades across China and analyze its influencing factors to provide the basis for further improvement of regular cleaning quality inspection. Methods Cross-sectional study was conducted form February to July of 2017,and 132 hospitals all over the country were selected to investigate with the questionnaire the current status of CSSD management model, staffing and regular cleaning quality inspection. Results The ratio of the nursing staff and the number of beds was 1.15:100,the ratio of staff and the number of beds was 2. 00:100. 81. 82% (n =108) of the hospitals adopted the centralized management model. The qualification rate for regular spot check execution was 87.12% (n=115). The results of multiple logistic regression showed that the centralized management model was more conducive to the regular spot check than the non-centralized management model (OR=4.71,95% CI:1.05-21.08). Conclusions The centralized management model proves positive for the implementation of regular spot checks;and CSSDs should pay more attention to the regular spot check of the cleaning quality.

Objective    To explore the diagnostic value of circulating tumor cells (CTC) measured by magnetic nanoparticle method in lung cancer. Methods    (1) We measured binding capability of A549 or NCI-H1965 cell lines with recognition peptide and capture efficiency by adding tumor cells into the whole blood of healthy human. (2) We measured CTC of 34 patients suspected with lung cancer, and the counting results of CTC were compared with the following pathological results. Results    (1) The binding capability was 80.0%±6.0% for A549 and 70.1%±4.8% for H1957, while the capture efficiency was 57.3%±7.0% for A549 and 37.3%±6.1% for H1975. (2) CTCs were identified in 71.9% of patients with lung cancer. The specificity was 83.3%, and area under receiver operating characteristic (ROC) curve was 0.792 (P=0.003). Conclusion    CTC measured by magnetic nanoparticle method has promising application in the diagnosis of lung cancer.

To develop a method for the determination of 18 elements such as Pb, Cu, As, Hg, Mn, Ni, & Tl in Panax notoginseng,Bulbus fritillariae thunbergii,Coix seed, Resina draconis, and to control the contents of heavy metal elements in Sanjie Zhentong Capsule, the samples were digested by microwaves and then analyzed by appropriate determination parameters through ICP-MS, with the internal standard method to improve the matrix effect and interference. The correlation coefficientR2≥ 0.999 2. The lowest limits of quantification were from 0.002 8 to 0.54 μg·L-1. The experiments had better repeatability, while the recovery values ranged from 73.01% to 109.13%. The method is simple, accurate and high sensitive, and it can be used for the determination of rapid monitoring the multi-elements inPanax notoginseng, Bulbus fritillariae thunbergii, Coix seed,and Resina draconis.