OBJECTIVE To investigate the effects and mechanisms of Buyang huanwu decoction （BYHWD） and its active fractions in ameliorating non-alcoholic fatty liver disease. METHODS BYHWD and its effective fractions obtained through ethanol precipitation， as well as 30% ethanol， 50% ethanol， and 75% ethanol fractions （namely， the CC effective fraction， 30YC effective fraction， 50YC effective fraction， and 75YC effective fraction）， were prepared. These preparations were administered to rats via intragastric administration to prepare corresponding drug-containing serum （blank serum and simvastatin-containing serum were prepared using the same protocol）. Human L02 hepatocytes were divided into control group， model group， simvastatin-containing serum group， BYHWD-containing serum group， CC-containing serum group， 30YC-containing serum group， 50YC-containing serum group， and 75YC-containing serum group. Except for the control group， other groups were given 0.2 mol/L oleic acid for 24 h to induce a lipid accumulation model， and then intervened with 20% drug-containing serum/blank serum for 24 h. The lipid deposition in cells was observed， and the proportion of lipid droplet area was calculated； the levels of triglycerides （TG） and indicators of oxidative stress [malondialdehyde （MDA）， superoxide dismutase （SOD）] as well as liver function [alanine amino- transferase （ALT）， aspartate amino-transferase （AST）] in cells were detected； protein and mRNA expressions of AMP-activated protein kinase （AMPK）/sterol regulatory element-binding protein-1 （SREBP-1）/glycerol-3-phosphate acyltransferase （GPAT） signaling pathway were also measured. RESULTS Compared with the control group， cells in the model group exhibited severe cellular steatosis， with a significantly increased proportion of lipid droplet area， as well as the elevated levels of TG， ALT， AST， and MDA in cells， along with significantly up-regulated mRNA and protein expression levels of SREBP-1 and GPAT （P＜0.05）. The level of SOD， mRNA expression of AMPK， as well as the protein phosphorylation level of AMPK were decreased significantly （P＜0.05）. Compared with the model group， cellular steatosis was alleviated in all drug-containing serum groups， and the levels of most of the aforementioned quantitative indicators were significantly reversed （P＜0.05）. CONCLUSIONS BYHWD and its active fractions can exert a therapeutic effect on improving non-alcoholic fatty liver disease by regulating the AMPK/SREBP-1/GPAT signaling pathway， inhibiting oxidative stress responses， and reducing lipid deposition.

Objective To investigate the expression of insulin-like growth factor 2 mRNA binding protein 1(IGF2BP1)mRNA and patrilineal expression of genetic imprinting gene 10(PEG10)mRNA in endometrial carcinoma(EC)tissues and their correlation with proliferation gene expression and prognosis.Methods A total of 100 EC patients diagnosed and treated in Hubei Institute of Technology Affiliated Maternal and Child Health Hospital from January 2017 to January 2019 were selected.The expression of IGF2BP1 mRNA,PEG 10 mRNA,proliferating cell nuclear antigen(PCNA),cyclin D1,and cyclin dependent kinase 4(CDK4)mRNA were examined by real-time fluorescence quantitative PCR.The expressions of IGF2BP1 and PEG 10 in tissues were detected by immunochemistry,and their correlation were analyzed by Pearson correlation analysis.Kaplan-Meier curve analysis were used to analyze the prognostic differences in EC patients with different IGF2BP1 and PEG 10 expression groups.COX regression was used to analyze the prognostic factors of EC patients.Results The expression levels of IGF2BP1 mRNA(1.84±0.33),PEG10 mRNA(2.12±0.40),PCNA mRNA(3.14±0.42),cyclinD1 mRNA(2.81±0.36)and CDK4 mRNA(2.37±0.34)in EC cancer tissues were higher than those in adjacent tissues(0.78±0.21,0.91±0.25,0.74±0.13,0.67±0.21,0.59±0.18),and the differences were significant(t=25.652～54.588,all P＜0.05).The positive rates of IGF2BP1(70.00％)and PEG 10(72.00％)in cancer tissues were higher than those in adjacent tissues(10.00％,9.00％),and the differences were statistically significant(x2=75.000,82.363,all P＜0.05).The expressions of IGF2BP1 mRNA and PEG10 mRNA in EC tissues were positively correlated with PCNA mRNA,cyclinD1 mRNA,and CDK4 mRNA(r=0.562～0.625,all P＜0.05).There was a significant positive correlation between IGF2BP1 mRNA and PEG10 mRNA expression in EC tissues(r=0.663,P＜0.05).The positive rates of IGF2BP1(86.49％,87.50％)and PEG10(89.19％,90.63％)protein expression in EC cancer tissues with FIGO stage Ⅲ and combined lymph node metastasis were higher than those in FIGO stage Ⅰ-Ⅱ(60.321％,61.90％)and without lymph node metastasis(61.77％,63.24％),and the differences were sttistically significant(x2=6.863～8.608,all P＜0.05).The overall 3-year survival rate of IGF2BP1 positive patients[70.00％(49/70)]was lower than that of IGF2BP1 negative patients[90.00％(27/30)],the overall 3-year survival rate of patients in PEG10 positive patients[69.40(50/72)]was lower than that in PEG10 negative patients[92.86％(26/28)],and the differences were statistically significant(Log rankx2=4.133,5.491,P=0.042,0.019).FIGO stage Ⅲ(OR=1.449,95％CI:1.148～1.830),combined lymph node metastasis(OR=1.442,95％CI:1.124～1.850),IGF2BP1 positive(OR=1.637,95％CI:1.239～2.163),and PEG10 positive(OR=1.576,95％CI:1.136～1.187)were independent risk factors affecting the survival and prognosis of EC patients(all P＜0.05).Conclusion The expressions of IGF2BP1 and PEG 10 in EC were increased,and they were positively correlated with the proliferation gene expression.They may be potential tumor markers for prognostic evaluation of EC.

Objective:Assessing the prognosis of patients with bladder urothelial carcinoma by using multiple molecular markers [epithelial-cadherin (E-cadherin), fibroblast growth factor receptor 3 (FGFR3), Jagged2, Survivin and stromal antigen 2 (STAG2)] in combination method, and compared it with the traditional method of evaluating prognosis by clinical pathological parameters.Methods:Retrospective analysis of 128 cases of bladder urothelial carcinoma patients admitted to Beijing Friendship Hospital, Capital Medical University from January 2010 to December 2016, including 102 males and 26 females; the median age was 70.5 years, ranged from 41 to 93 years. E-cadherin, FGFR3, Jagged2, Survivin and STAG2 alterations by immunohistochemistry during the first surgical treatment. The Kaplan-Meier survival curve was used to evaluate the relationship between the above markers and overall survival (OS), recurrence-free survival (RFS), progression-free survival (PFS), and clinicopathological indicators of tumors. Use Cox regression model to find the most suitable molecular markers for judging the prognosis of bladder urothelial carcinoma, and compare it with the traditional clinical staging + pathological grading method to evaluate OS to detect its sensitivity and specificity.Results:After 36.4 months of follow-up, it was found that the expressions of E-cadherin, FGFR3, Jagged2 and Survivin were all related to the OS, RFS and PFS of bladder urothelial carcinoma (all P<0.05). The expression of STAG2 was related to the TMN stage of bladder urothelial carcinoma ( P=0.047) and pathological grade ( P=0.015). Cox regression analysis showed that Survivin ( P=0.001) and Jagged2 ( P=0.037) were independent risk factors for evaluating the OS of bladder urothelial carcinoma, and Survivin ( P<0.001) and Jagged2 ( P=0.006) were independent risk factors for RFS, Survivin ( P=0.001) was also an independent risk factor for PFS. Multivariate analysis of the above molecular markers showed that the prognosis of patients with more than 3 molecular markers was better than that of independent application or the use of two of them to evaluate the prognosis ( P<0.001). The combined application of Survivin and Jagged2 to evaluate the 5-year survival rate was not less sensitive and specific than the clinical and pathological indicators (93.5% vs 77.2%, 84.7% vs 81.3%). Conclusions:Five molecular markers of E-cadherin, FGFR3, Jagged2, Survivin and STAG2 have an evaluation effect on the prognosis of bladder urothelial carcinoma, and some can independently predict the OS and RFS of patients with bladder urothelial carcinoma, however, the combined application is better than the single molecular marker to evaluate the prognosis. Compared with the traditional method of evaluating the prognosis by clinical pathological parameters, the combined application of Jagged2 and Survivin may be a better choice for evaluating the prognosis of patients with bladder urothelial carcinoma.

Objective To investigate the interaction between myocardial norepinephrine (NET) and protein interacting with kinase Cα (PICK1),and examine the myocardial expression pattern of NET and PICK1 in mice with adriamycin-induced congestive heart failure.Methods (1) Cellular experiments: 293T cells were transfected with NET,GFP-PICK1,NET + GFP-PICK1 or NET + GFP-PICK1 (KD-AA),respectively.Immunofluorescence staining was performed 48 h after the transfection.(2) Animal experiments: 40 male C57BL/6J mice were divided into control group and adriamycin group (intraperitoneal injection of 2 mg/kg adriamycin with a cumulative amount of 22 mg/kg).The myocardial mRNA and protein expression level of NET,PICK1 and adrenergic receptor (β1-AR) were detected by real-time PCR and Western blot after 10 weeks.Results (1) PICK1 mediates the intracellular trafficking of NET.(2) Compared to controls,cardiac mRNA expression of NET remained unchanged,but PICK1 and β1-AR mRNA level were significantly reduced in the heart failure mice.(3) Myocardial NET protein expression level was significantly reduced,whereas tyrosine hydroxylase (TH) protein expression was significantly upregulated in heart failure mice.(4) The myocardial density of sympathetic nerve fibers remained unchanged in heart failure mice.Conclusions Cardiac expression of NET and PICK1 are down-regulated in heart failure mice.Reduced PICK1-mediated intracellular trafficking of NET may be involved in the impairment of NET function in this congestive heart failure mice model.

Objective To evaluate the effects of iron dextran or iron sucrose on the stability of fat emulsion in total nutrient admixture (TNA) in pediatric settings.Methods TNA with different intravenous doses of iron sucrose or iron dextran (0.25,0.5,0.75,or 1.00 mg) were prepared,and each dose was prepared 10 bags.The TNAs were stored at 25 ℃ for 3 days,and the stability of fat emulsion was observed by electron scanning microscopy.Meanwhile,the pH and osmolality were also measured.Results The particle sizes of fat emulsions in TNA with different concentrations of iron sucrose or iron dextran at different time points were not significantly different (F =0.32,P =0.7836 ; F =1.73,P =0.1321,respectively).The mean particle size of the fat emulsion in each group was ＜ 0.5 μm within 72 hours.For TNA containing different concentrations of iron,the percentage of particles ＞ 0.5 μm,pH,and osmotic pressure showed no significant difference at different time points (percentage:F =1.47,P =0.3467 ; F =1.04,P =0.4758.pH:F =0.63,P =0.5942 ; F =0.46,P =0.6825.osmotic pressure:F =1.37,P =0.3648 ; F =0.65,P =0.6023).Conclusion The TNA addeded with iron sucrose or iron dextran with an concentrations of ＜ 1％ is stable.

Forty-one type 2 diabetic patients with fasting plasma glucose≥15 mmol/L and postprandial plasma glucose≥16.8 mmol/L underwent short-term intensive insulin treatment (IIT). Glucose load tests were performed before and after treatment in these patients and proinsulin and C peptide were assayed by RIA. The results showed that IIT decreased the levels of fasting and postprandial proinsulin and improved the ? cell function.