Objective To study the effect and possible mechanism of angiotensin Ⅱ receptor blocker(ARB)on energy metabolism in mice.Methods ① Male 6-week-old C57BL/6N mice were fed with normal diet(ND)and high-fat diet(HFD)for 6 weeks,then detect their body weight and fasting blood glucose.② The diabetic mice induced by high-fat diet were randomly divided into control group(Con)and drug treatment group(ARB).They were administered with saline and valsartan at a dose of 3 mg/kg per day via gastric lavage for 6 weeks.After that,the body weight,body temperature,intraperitoneal glucose tolerance test,respiratory metabolism rate,hematoxylin-eosin staining of adipose tissue,and the expression of thermogenesis-related genes in brown adipose tissue were examined.③By using male 6-week-old db/db mice as the experimental subjects,they were also administered with saline and valsartan at a dose of 3 mg/kg per day via gastric lavage for 5 weeks.After that,the body weight,fasting blood glucose,and body temperature were measured.Results High-fat diet successfully induced the diabetic mouse model.Valsartan treatment significantly reduced the weight gain rate of HFD mice and the body weight of db/db mice,increased the body temperature of HFD mice in a 4℃cold environment,and improved the energy expenditure of HFD mice,but had no significant improvement on glucose metabolism in HFD mice.Compared with the control group,the fasting blood glucose level of db/db mice was significantly reduced after valsartan treatment,and the body temperature showed an upward trend after 2 h and 3 h of cold treatment at 4 ℃,but the difference was not statistically significant.In addition,for HFD mice,valsartan treatment significantly reduced the cell volume of brown adipocyte,subcutaneous adipocyte,and epididymal adipocyte.At the mRNA level,valsartan promoted the expression of UCP1 in brown adipose tissue,while the expression of Prdm16,PGC1α and AP2 expression did not change significantly.Conclusions Valsartan can improve energy metabolism in obese mice,and its mechanism may be related to the positive effects of ARB on the thermogenesis of brown adipose tissue.

Objective To study the effect and possible mechanism of angiotensin Ⅱ receptor blocker(ARB)on energy metabolism in mice.Methods ① Male 6-week-old C57BL/6N mice were fed with normal diet(ND)and high-fat diet(HFD)for 6 weeks,then detect their body weight and fasting blood glucose.② The diabetic mice induced by high-fat diet were randomly divided into control group(Con)and drug treatment group(ARB).They were administered with saline and valsartan at a dose of 3 mg/kg per day via gastric lavage for 6 weeks.After that,the body weight,body temperature,intraperitoneal glucose tolerance test,respiratory metabolism rate,hematoxylin-eosin staining of adipose tissue,and the expression of thermogenesis-related genes in brown adipose tissue were examined.③By using male 6-week-old db/db mice as the experimental subjects,they were also administered with saline and valsartan at a dose of 3 mg/kg per day via gastric lavage for 5 weeks.After that,the body weight,fasting blood glucose,and body temperature were measured.Results High-fat diet successfully induced the diabetic mouse model.Valsartan treatment significantly reduced the weight gain rate of HFD mice and the body weight of db/db mice,increased the body temperature of HFD mice in a 4℃cold environment,and improved the energy expenditure of HFD mice,but had no significant improvement on glucose metabolism in HFD mice.Compared with the control group,the fasting blood glucose level of db/db mice was significantly reduced after valsartan treatment,and the body temperature showed an upward trend after 2 h and 3 h of cold treatment at 4 ℃,but the difference was not statistically significant.In addition,for HFD mice,valsartan treatment significantly reduced the cell volume of brown adipocyte,subcutaneous adipocyte,and epididymal adipocyte.At the mRNA level,valsartan promoted the expression of UCP1 in brown adipose tissue,while the expression of Prdm16,PGC1α and AP2 expression did not change significantly.Conclusions Valsartan can improve energy metabolism in obese mice,and its mechanism may be related to the positive effects of ARB on the thermogenesis of brown adipose tissue.

Objective To explore the clinicopathological and immunohistochemical features of nephrogenic adenoma(NA).Methods Clinical data of NA patients diagnosed in the Department of Pathology,Zhongshan Hospital,Fudan University from July 2016 to October 2022 were collected and analyzed to explore their clinicopathological features.Results A total of 13 NA cases were enrolled.There were 11 males and 2 females.Organs involved:ureter(n=7),bladder(n=5),bladder and ureter(n=1),renal pelvis(n=2).NA patients performed as ureteral stenosis(6/7),rough bladder wall(3/5),and renal pelvis polyp(2/2).The typical microscopical features of NA were tubular(13/13)and papillary(4/13)structures,covered with cuboidal or columnar epithelium(13/13),or a mixed hobnail-spike eosinophilic epithelium(12/13);the interstitium was loose,containing varied amounts of vasculature and inflammatory cells(13/13).Immunohistochemistry revealed specific expressions of CK7,PAX-8,CK19 and CK8.Conclusions NA is a rare neoplasm of the urinary system with unique histological features.NA has the risk of misdiagnosis and over-treatment,and the potential of recurrence and malignant conversion.The diagnosis of NA depends on pathology,and the immunohistochemistry can be helpful for its pathological diagnosis.

Objective:To purify H5N1 influenza virus concentrate prepared by MDCK cells with a new mixed-mode chromatography medium Capto Core700 and the traditional medium Sepharose 4FF, and to compare the separation and purification efficacy of the two media.Methods:Capto Core700 and Sepharose 4FF were used to purify inactivated H5N1 influenza virus concentrate. The morphology of virus particles in different samples was then observed under a transmission electron microscope. Single radial immunodiffusion (SRID), Folin-Phenol (Lowry) method, double-antibody sandwich ELISA and qPCR were used to detect hemagglutinin, total protein, host cell protein (HCP) and host cell DNA (HCD) before and after purification. The recovery rate of virus antigen and the removal rate of impurities were calculated. The immunogenicity of the viruses purified with different media was analyzed using animal experiments. Difference in the purification efficacy of the two chromatography media was analyzed by t-test. Results:H5N1 influenza viruses purified by Capto Core700 or Sepharose 4FF showed the typical influenza virus morphology under transmission electron microscope. There was no significant difference in the recovery rate of hemagglutinin between the two chromatography media ( P>0.05), but compared with Sepharose 4FF, Capto Core700 had a higher removal rate of impurities (total protein, HCP, HCD) and the difference was statistically significant ( P<0.05). Animal experiments showed that the viruses purified by the two chromatography media had good immunogenicity. Conclusions:Compared with Sepharose 4FF chromatography medium, Capto Core700 could more effectively remove process-related impurities such as HCP, HCD and total protein without affecting the recovery rate of viral antigen. This study provided reference for the development of purification technology in the production of H5N1 influenza virus vaccine in MDCK cells.

OBJECTIVETo investigated the curative effect of autologous hematopoietic stem cell transplantation (ASCT) for malignant lymphoma.

METHODSThe clinical data of 81 patients with malignant lymphoma received ASCT from April 1999 to October 2013 were retrospectively analyzed. Of 81 patients, 70 were non-Hodgkin's lymphoma (NHL) and 11 Hodgkin's lymphoma (HL). High dose of etoposide combined with G-CSF was used to mobilize peripheral hematopoietic stem cell. Preconditioning regimen was BEAM (carmustine + cytarabine + etoposide + melphalan).

RESULTSEnough peripheral blood stem cells were collected from all patients. All of the patients after transplantation achieved hematopoietic reconstitution, the median time of the absolute neutrophil count (ANC) recovery to >0.5×10⁹/L time was 10(7-16) d, and the median time of platelet count recovery to >20×10⁹/L was 10(6-17) d. With the follow-up of 23(2-139) months, progression free survival (PFS) was 72.7%, and overall survival (OS) was 88.6%. The median PFS and OS were not reached. Complete remission (CR) before ASCT was an independent prognostic factor of PFS. No transplant related death happened.

CONCLUSIONASCT was a safe and effective method for treatment of malignant lymphoma.

Adolescent ; Adult ; Aged ; Female ; Hematopoietic Stem Cell Transplantation ; Humans ; Lymphoma ; therapy ; Male ; Middle Aged ; Retrospective Studies ; Transplantation, Autologous

Objective:To investigate the predictive value of serumal retinol-binding protein 4(RBP4) level fro fetal macrosomia of non-diabetic pregnant women .Methods :The serumal levels of RBP4 of 500 non-diabetic pregnant women at 12 week ,20 week and 24 week of pregnancy were measured by immune projection turbidimetric method .Fetal macrosomia was defined as birth weight≥4000 g .The cut-off value ,sensitivity and specificity were calculated with receiver operating characteristic (ROC) curve .Results:Of the 500 non-diabetic pregnant women ,30 cases(6% ) got fetal macrosomia .The ROC curve showed that the predictive cut-off values of RBP4 at 12 week ,20 week and 24 week of pregnancy were 61 .0 mg/L ,50 .5 mg/L and 52 .5 mg/L , respectively ;the predictive sensitivity and specificity at 12 week ,20 week and 24 week of pregnancy were 42 .9% and 94 .5% , 70 .0% and 69 .5% ,76 .9% and 73 .2% ,respectively .The predictive cut-off value of RBP4 no later than 24 week of pregnancy was 51 .5 mg/L ;the predictive sensitivity and specificity were 61 .8% and 69 .5% .There was significant difference(P<0 .05) between the serumal level of RBP4 at 24 week of pregnancy in group fetal macrosomia and that in group nonfetal macrosomia . Conclusions :The predictive sensitivity of RBP4 increases in accordance with the increase of serumal level of RBP 4 .The serumal level of RBP4 of non-diabetic pregnant women at 24 week of pregnancy may have higher sensitivity and specificity in the predic-tion of fetal macrosomia .If the serumal level of RBP4 no later than 24 week of pregnancy is beyond 50 mg/L ,then the risk of fetal macrosomia will be higher .