Objective　To detect clustered regularly interspaced short palindromic repeats (CRISPR) in Listeria monocytogenes, and analyze the structure and homology of CRISPR loci. Methods Totally 34 strains of Listeria monocytogenes isolated in our laboratory were identified, PCR amplified and sequenced. The repeat sequence structure and spacer sequence homology in CRISPR loci were analyzed by bioinformatics software. Results A total of 7 CRISPR loci were detected in 34 strains. The mutation rate of the first 2 and last 2 bases of the Repeat sequence of CRISPR loci was higher, while the mutation rate of the middle part was lower. Seven CRISPR sites form eight CRISPR structural types, among which the Repeat sequences of CRISPR1 and CRISPR2 are relatively conserved, while the Repeat sequences of CRISPR1 and CRISPR5 can form dumbbell shaped secondary structures. The number of Spacer sequences contained in each CRISPR site ranges from 2 to 15, with an average of 2.43. The 136 Spacer sequences detected were not only homologous to Listeria plasmids and bacteriophages, but also homologous to uncultured virus sequences, staphylococcal bacteriophages, and Listeria innocua. The same CRISPR genotype did not show large-scale clustering, but some strains in the same year were in the same evolutionary cluster with close genetic relationships. Conclusion The CRISPR structure of Listeria monocytogenes in this study exhibits high specificity, and its homology with bacteriophages provides a theoretical basis for the application of bacteriophages in the control and prevention of Listeria monocytogenes.

Focusing on the task requirements of high-quality development of public hospitals,based on literature research and comprehensive budget management practice of the sample hospital,it analyzes the current situation of comprehensive budget management of public hospitals,explores possible problems and solutions in the process of comprehensive budget management system construc-tion,and puts forward the general idea of comprehensive budget management system construction,specific steps and processes for implementation.In the process of the construction and innovation of the comprehensive budget management system,public hospitals in prefecture-level cities should adapt to local conditions,be goal-oriented,scientifically use various management tools,deeply deepen the concept of budget,promote the upgrading of information system,and finally form a four-part integrated system of bud-get,finance,cost and performance.

Focusing on the task requirements of high-quality development of public hospitals,based on literature research and comprehensive budget management practice of the sample hospital,it analyzes the current situation of comprehensive budget management of public hospitals,explores possible problems and solutions in the process of comprehensive budget management system construc-tion,and puts forward the general idea of comprehensive budget management system construction,specific steps and processes for implementation.In the process of the construction and innovation of the comprehensive budget management system,public hospitals in prefecture-level cities should adapt to local conditions,be goal-oriented,scientifically use various management tools,deeply deepen the concept of budget,promote the upgrading of information system,and finally form a four-part integrated system of bud-get,finance,cost and performance.

Focusing on the task requirements of high-quality development of public hospitals,based on literature research and comprehensive budget management practice of the sample hospital,it analyzes the current situation of comprehensive budget management of public hospitals,explores possible problems and solutions in the process of comprehensive budget management system construc-tion,and puts forward the general idea of comprehensive budget management system construction,specific steps and processes for implementation.In the process of the construction and innovation of the comprehensive budget management system,public hospitals in prefecture-level cities should adapt to local conditions,be goal-oriented,scientifically use various management tools,deeply deepen the concept of budget,promote the upgrading of information system,and finally form a four-part integrated system of bud-get,finance,cost and performance.

Focusing on the task requirements of high-quality development of public hospitals,based on literature research and comprehensive budget management practice of the sample hospital,it analyzes the current situation of comprehensive budget management of public hospitals,explores possible problems and solutions in the process of comprehensive budget management system construc-tion,and puts forward the general idea of comprehensive budget management system construction,specific steps and processes for implementation.In the process of the construction and innovation of the comprehensive budget management system,public hospitals in prefecture-level cities should adapt to local conditions,be goal-oriented,scientifically use various management tools,deeply deepen the concept of budget,promote the upgrading of information system,and finally form a four-part integrated system of bud-get,finance,cost and performance.

Focusing on the task requirements of high-quality development of public hospitals,based on literature research and comprehensive budget management practice of the sample hospital,it analyzes the current situation of comprehensive budget management of public hospitals,explores possible problems and solutions in the process of comprehensive budget management system construc-tion,and puts forward the general idea of comprehensive budget management system construction,specific steps and processes for implementation.In the process of the construction and innovation of the comprehensive budget management system,public hospitals in prefecture-level cities should adapt to local conditions,be goal-oriented,scientifically use various management tools,deeply deepen the concept of budget,promote the upgrading of information system,and finally form a four-part integrated system of bud-get,finance,cost and performance.

Focusing on the task requirements of high-quality development of public hospitals,based on literature research and comprehensive budget management practice of the sample hospital,it analyzes the current situation of comprehensive budget management of public hospitals,explores possible problems and solutions in the process of comprehensive budget management system construc-tion,and puts forward the general idea of comprehensive budget management system construction,specific steps and processes for implementation.In the process of the construction and innovation of the comprehensive budget management system,public hospitals in prefecture-level cities should adapt to local conditions,be goal-oriented,scientifically use various management tools,deeply deepen the concept of budget,promote the upgrading of information system,and finally form a four-part integrated system of bud-get,finance,cost and performance.

Focusing on the task requirements of high-quality development of public hospitals,based on literature research and comprehensive budget management practice of the sample hospital,it analyzes the current situation of comprehensive budget management of public hospitals,explores possible problems and solutions in the process of comprehensive budget management system construc-tion,and puts forward the general idea of comprehensive budget management system construction,specific steps and processes for implementation.In the process of the construction and innovation of the comprehensive budget management system,public hospitals in prefecture-level cities should adapt to local conditions,be goal-oriented,scientifically use various management tools,deeply deepen the concept of budget,promote the upgrading of information system,and finally form a four-part integrated system of bud-get,finance,cost and performance.

Focusing on the task requirements of high-quality development of public hospitals,based on literature research and comprehensive budget management practice of the sample hospital,it analyzes the current situation of comprehensive budget management of public hospitals,explores possible problems and solutions in the process of comprehensive budget management system construc-tion,and puts forward the general idea of comprehensive budget management system construction,specific steps and processes for implementation.In the process of the construction and innovation of the comprehensive budget management system,public hospitals in prefecture-level cities should adapt to local conditions,be goal-oriented,scientifically use various management tools,deeply deepen the concept of budget,promote the upgrading of information system,and finally form a four-part integrated system of bud-get,finance,cost and performance.

Infrared thermography is an advanced technology that utilizes infrared detectors to sense the infrared thermal radiation emitted from the human body' s surface, converting it into electrical signals. These signals are then processed by computers to generate planar temperature color images, providing an intuitive display of the body surface temperature distribution. Rheumatic diseases, as prevalent conditions that significantly impact the quality of life of millions of people worldwide, pose significant challenges in diagnosis and assessment. Traditional diagnostic and evaluation methods, while possessing certain clinical value, exhibit non-negligible limitations. With the advancements and increasing popularity of infrared thermography technology, combined with the progress in medical image recognition and artificial intelligence algorithms, this technology has demonstrated increasingly prominent advantages in the field of rheumatology. By employing this non-invasive and highly efficient technique for analyzing superficial tissue thermal radiation distribution, early and precise diagnosis of rheumatic diseases, as well as dynamic monitoring of disease progression, becomes feasible. This technological advancement enhances the accuracy and efficiency of rheumatic disease diagnosis, thereby alleviating the burden on healthcare systems and optimizing the allocation of medical resources. Furthermore, infrared thermography introduces new vitality into the diagnosis and treatment monitoring of rheumatic diseases. It enables clinicians to detect subtle changes in body surface temperature that may indicate underlying inflammatory or metabolic processes associated with rheumatic conditions. This capability facilitates early intervention and personalized treatment strategies, ultimately contributing to improved patient outcomes and satisfaction. The integration of infrared thermography with advanced image processing algorithms and artificial intelligence further amplifies its potential, enabling automated analysis and interpretation of thermal images, thus reducing the dependence on manual interpretation and enhancing the reproducibility and reliability of diagnostic results. In conclusion, infrared thermography represents as a promising tool in the management of rheumatic diseases, offering a non-invasive, cost-effective, and efficient means for early diagnosis, monitoring, and therapeutic evaluation of diseases. As technology continues to evolve, the application of infrared thermography is expected to advance further in rheumatic diseases.
Thermography/methods* ; Humans ; Rheumatic Diseases/diagnosis* ; Infrared Rays ; Algorithms