Objective To explore the mechanism of hepatic oval cells(HOCs)regulating liver regener-ation through Wnt/β-catenin pathway in human liver cirrhosis.Methods Forty cases of Child-Pugh class A cirrhosis tissues and 10 cases of normal liver tissues were collected and divided into the normal liver tissue group,the liver fibrosis group,the mild cirrhosis group and the moderate cirrhosis group based on HE staining and the Laennec classification system.The expression of Wnt/β-catenin pathway,CyclinD1 and Jagged-1 in each group were detected and compared,and the mechanism of Wnt/β-catenin pathway in the regulation of liver regeneration by HOCs was explored.Results The expression of Wnt and β-catenin increased with the aggravation of liver cirrhosis(P＜0.05).In contrast,the expression of CyclinD1 and Jagged-1 decreased with the aggravation of liver cirrhosis(P＜0.05).The expression levels of Wnt and β-catenin were positively corre-lated with the number of HOCs per unit area(r=0.806,0.634,P＜0.01).Compared with the degree of liver cirrhosis,the expression of Wnt/β-catenin had greater influence on the number of HOCs per unit area,the ex-pression of CyclinD1 and Jagged-1,and the differences were statistically significant(P＜0.05).Conclusion Wnt/β-catenin pathway can promote the generation of HOCs in human liver cirrhosis and mediate liver regenera-tion.

Objectives:Quantitative flow ratio(QFR)is a coronary angiography-derived functional test without the need of guidewire use.Fractional flow reserve(FFR)is used as the reference standard to verify the diagnostic value of QFR in patients with non-ST-segment elevation acute coronary syndrome(NSTE-ACS)with coronary critical lesion(40%-70%stenosis)and functional stenosis. Methods:This retrospective analysis included patients with NSTE-ACS who were admitted to Fuwai Central China Cardiovascular Hospital from June 1,2018 to February 1,2023 and underwent coronary FFR examination.QFR values of target vessels were analyzed offline by AngioPlus(Shanghai Pulsation Medical Imaging Technology Co.,LTD.),the second-generation QFR detector,and anatomical parameters of the diseased vessels were recorded as follows:minimal luminal diameter(MLD),percent diameter stenosis(DS%),minimal luminal area(MLA),percent area stenosis(AS%).Functional coronary artery stenosis is defined as FFR≤0.80. Results:Using FFR as the gold standard,the AUC values of contrast-flow QFR(cQFR)and fixed-flow QFR(fQFR)for identifying functional coronary artery stenosis in NSTE-ACS patients were 0.829(95%CI:0.773-0.885,P<0.001)and 0.821(95%CI:0.766-0.875,P<0.001),respectively.The diagnostic accuracy,sensitivity and specificity of cQFR and fQFR were 81.30%,56.00%,98.63%and 76.83%,59.00%,99.04%,respectively.DeLong test showed that diagnostic performance of cQFR was significantly better than fQFR in diagnosing functional stenosis of coronary critical lesions in patients with NSTE-ACS. Conclusions:With FFR as the gold standard,QFR(especially cQFR)has certain diagnostic value in patients with NSTE-ACS with functional stenosis of coronary critical lesions.

Objective With the popularization of low dose chest CT examination,a large number of patients with pulmonary nodules(10 mm ＜ D≤20 mm) or small nodules(4 mm ＜ D≤10 mm) emerged.How to accurately position pulmonary nodules especially small nodules in video-assisted thoracoscopic surgery(VATS) is the key.This study explores the positioning method for small pulmonary nodules in VATS.Methods From January 2012 to June 2014,we used a method named clock dial integrated positioning in 316 patients with pulmonary nodules who underwent limited resection or lobectomy in VATS,obtained satisfactory results.Results The accuracy of clock dial integrated positioning method in judgments was 99％ (313/316).296 cases underwent thoracoscopic sublobectomy,including thoracoscopic pulmonary segmental resection in 141 cases and thoracoscopic wedge resection in 155 cases.20 cases underwent thoracoscopic lobectomy.Among them 17 cases had multinodules which were in the different pulmonary segments of the same lobe.Underwent thoracoscopic lobectomy.The positions of 3 cases were error during early cases,underwent VATS lobectomy.The postoperative pathology:194 cases were lung cancer.37 cases were metastatic tumor.85 cases were benign lesions.Conclusion Combined with the clinical treatment experience,we believe that the clock dial integrated positioning method is effective for positioning in VATS.Clinical VATS experiences accumulations are also needed.

Objective To analyze the space-time characteristic of traumatic brain injury (TBI) caused by Chinese Wenchuan earthquake and discuss opportunity and mode of medical intervention. Methods A retrospective study was done on 92 patients with TBI admitted into our department during Chinese Wanchuan earthquake.Results All 92 patients came from champaign located at northwest of Chengdu. Of all, 76 patients were admitted within 12 hours after earthquake. Surgical operation Wag done on 10 patients, of whom 3 died.Conclusions In the early period after earthquake, TBI patients make a large proportion and have fast change of injury severity. All patients come from champaign with conven-ient transportation. For TBI patients, early emergent operation after admission can significantly elevate success rate of mcdical intervention.

Objective To evaluate the hemocompatibility of domestic silicone-covered stent in the iliac arteries of canine model. Methods Eighteen domestic stents were placed in ililac arteries of 9 adult dogs after larger ballon PTA, which included 10 silicone-covered stents and 8 bare stents for control. DSA was performed at 1,4,12 weeks after stent implantation in the iliac arteries of two groups to observe the outcomes of patency or restenosis. Animals were then euthanized isolating and stainning the stented arteries with hematoxylin and eosin for histological examination. Finally, the acute thrombosis, reendothelialization and the neointimal proliferation of both covered and bare stents were quantified on histological cross-section. Results All bare stents were patent in 12 weeks, but two silicone-covered stents were occluded at 4,12 week respectively (patent rate was 80%). Stented vascular stenosis rate was averaging 72.3% at 12 week in covered stents and 36.7% in bare stents. Conclusions The hemocompatibility of silicone-covered stents is not better than that of bare stents. Silicone appear to be inert in this experimental application.