Objective The study aimed to explore the clinical diagnostic value of D-Dimer(D-D)com-bined with N-terminal pro-B-type natriuretic peptide(NT-proBNP)in the occurrence of renal dysfunction in patients with acute coronary syndrome(ACS).Methods As a retrospective cohort study,we selected 682 patients as the research subjects.The patients were with acute coronary syndrome who visited the Chest Pain Center of Tianjin First Central Hospital during January 2021 and December 2023.Based on the eGFR values calculated from the creatinine levels before coronary angiography,patients were divided into the renal insufficiency group and the normal renal function group,comprising 102 cases and 580 cases,respectively.Binary logistic regression was adopted to analyze the influence factors of renal dysfunction in ACS patients,and the receiver operating characteristic(ROC)curve was utilized to evaluate the diagnostic value of D-D and NT-proBNP levels for renal dysfunction in ACS patients.Results The levels of D-D and NT-proBNP in the renal insufficiency group were significantly higher than those in the normal renal function group(P<0.05).Univariate logistic regression analysis showed that the rise of D-D and NT-proBNP levels were both independent risks for renal dysfunction in ACS patients.Multivariate logistic regression analysis indicated that the combined detection of D-D and NT-proBNP was an independent risk factor for renal dysfunction in ACS patients.The ROC results showed that the AUC,sensitivity,and specificity of the D-D level in diagnosing renal dysfunction in ACS patients were 0.805,58.82%,and 100%,respectively.The AUC,sensitivity,and specificity of NT-proBNP level in diagnosing renal dysfunction after myocardial infarction in ACS patients were 0.737,67.65%,and 73.62%,respectively.The AUC,sensitivity,and specificity of D-D combined with NT-proBNP in diagnosing renal dysfunction in ACS patients were 0.838,68.63%,and 93.28%,respectively.The diagnostic value of combined detection was superior to that of individual testing.Conclusions The levels of D-D and NT-proBNP were significantly elevated in ACS patients with renal dysfunction,which were independent risk factors for the occurrence of renal dysfunction,and the combined detection could help diagnose renal dysfunction.

Objective To investigate the neuroprotective effect of Dendrobium nobile Lindl(DNL)extract in a Caenorhabditis elegans(C.elegans)model of Parkinson's disease(PD).Methods C.elegans NL5901 and 6-hydroxydopamine(6-OHDA)induced N2,BZ555,PD4521,and CB7272 C.elegans strains were treated with DNL 7.5,15,and 30 mg/L.The survival rate,basal slowing response rate,α-synuclein(α-syn)aggregation,dopaminergic neurons(DNs),mitochondrial distribution density of body wall muscle cells,and protein levels in the membrane were observed.In addition,reactive oxygen species(ROS),superoxide dismutase(SOD)and glutathione(GSH)in 6-OHDA induced N2 was detected to explore the effect of DNL on the antioxidative stress ability of PD C.elegans models.Results Compared with that in the model group,the DN fluorescence intensity was significantly increased in nematodes treated with DNL and levodopa(L-DOPA)(P＜0.05,P＜0.0001),α-syn aggregation was significantly decreased(P＜0.05,P＜0.001,P＜0.0001),the basal slowing rate(P＜0.05,P＜0.01,P＜0.001),mitochondrial density(P＜0.05,P＜0.01,P＜0.001),mitochondrial intima protein content(P＜0.05,P＜0.001,P＜0.0001),SOD content(P＜0.05),and GSH content were all increased.The ROS content was reduced in nematodes(P＜0.01).The lifespans of N2 wild-type and PD C.elegans models were prolonged after DNL treatment(P＜0.05,P＜0.01,P＜0.001).Conclusions DNL can effectively improve motor paralysis in a C.elegans PD model,improve DN degradation,inhibit α-syn aggregation and neuronal damage,increase the antioxidative stress ability,and slow the aging process in C.elegans.

Objective To investigate the neuroprotective effect of Dendrobium nobile Lindl(DNL)extract in a Caenorhabditis elegans(C.elegans)model of Parkinson's disease(PD).Methods C.elegans NL5901 and 6-hydroxydopamine(6-OHDA)induced N2,BZ555,PD4521,and CB7272 C.elegans strains were treated with DNL 7.5,15,and 30 mg/L.The survival rate,basal slowing response rate,α-synuclein(α-syn)aggregation,dopaminergic neurons(DNs),mitochondrial distribution density of body wall muscle cells,and protein levels in the membrane were observed.In addition,reactive oxygen species(ROS),superoxide dismutase(SOD)and glutathione(GSH)in 6-OHDA induced N2 was detected to explore the effect of DNL on the antioxidative stress ability of PD C.elegans models.Results Compared with that in the model group,the DN fluorescence intensity was significantly increased in nematodes treated with DNL and levodopa(L-DOPA)(P＜0.05,P＜0.0001),α-syn aggregation was significantly decreased(P＜0.05,P＜0.001,P＜0.0001),the basal slowing rate(P＜0.05,P＜0.01,P＜0.001),mitochondrial density(P＜0.05,P＜0.01,P＜0.001),mitochondrial intima protein content(P＜0.05,P＜0.001,P＜0.0001),SOD content(P＜0.05),and GSH content were all increased.The ROS content was reduced in nematodes(P＜0.01).The lifespans of N2 wild-type and PD C.elegans models were prolonged after DNL treatment(P＜0.05,P＜0.01,P＜0.001).Conclusions DNL can effectively improve motor paralysis in a C.elegans PD model,improve DN degradation,inhibit α-syn aggregation and neuronal damage,increase the antioxidative stress ability,and slow the aging process in C.elegans.

Objective The study aimed to explore the clinical diagnostic value of D-Dimer(D-D)com-bined with N-terminal pro-B-type natriuretic peptide(NT-proBNP)in the occurrence of renal dysfunction in patients with acute coronary syndrome(ACS).Methods As a retrospective cohort study,we selected 682 patients as the research subjects.The patients were with acute coronary syndrome who visited the Chest Pain Center of Tianjin First Central Hospital during January 2021 and December 2023.Based on the eGFR values calculated from the creatinine levels before coronary angiography,patients were divided into the renal insufficiency group and the normal renal function group,comprising 102 cases and 580 cases,respectively.Binary logistic regression was adopted to analyze the influence factors of renal dysfunction in ACS patients,and the receiver operating characteristic(ROC)curve was utilized to evaluate the diagnostic value of D-D and NT-proBNP levels for renal dysfunction in ACS patients.Results The levels of D-D and NT-proBNP in the renal insufficiency group were significantly higher than those in the normal renal function group(P<0.05).Univariate logistic regression analysis showed that the rise of D-D and NT-proBNP levels were both independent risks for renal dysfunction in ACS patients.Multivariate logistic regression analysis indicated that the combined detection of D-D and NT-proBNP was an independent risk factor for renal dysfunction in ACS patients.The ROC results showed that the AUC,sensitivity,and specificity of the D-D level in diagnosing renal dysfunction in ACS patients were 0.805,58.82%,and 100%,respectively.The AUC,sensitivity,and specificity of NT-proBNP level in diagnosing renal dysfunction after myocardial infarction in ACS patients were 0.737,67.65%,and 73.62%,respectively.The AUC,sensitivity,and specificity of D-D combined with NT-proBNP in diagnosing renal dysfunction in ACS patients were 0.838,68.63%,and 93.28%,respectively.The diagnostic value of combined detection was superior to that of individual testing.Conclusions The levels of D-D and NT-proBNP were significantly elevated in ACS patients with renal dysfunction,which were independent risk factors for the occurrence of renal dysfunction,and the combined detection could help diagnose renal dysfunction.

Mitochondrial tRNAgene mutation is closely related to acoustic nerve deafness. Some mutations can affect the structure and transcriptional processing of tRNA, for instance m.7444G>A mutation in tRNAprecursor 3' side, m.7472 insC as well as m.7511T>C mutations in the stem and ring of tRNA, may influence tRNAstability, thus affect the synthesis of mitochondrial peptides, reduce the production of ATP and cause deafness. This article focuses on mitochondrial tRNAgene mutations as well as the mechanism underlying hearing loss.
Amino Acid Sequence ; Base Sequence ; Genetic Predisposition to Disease ; genetics ; Hearing Loss ; genetics ; Humans ; Mitochondrial Proteins ; biosynthesis ; genetics ; Mutation ; Nucleic Acid Conformation ; RNA ; chemistry ; genetics ; RNA, Transfer, Ser ; chemistry ; genetics