Objective To detect the expression of N-acetyltransferase 10(NAT10)and polyadenylate bind-ing protein cytoplasmic 1(PABPC1)in non muscle invasive bladder cancer(NMIBC),and analyze the correla-tion between them and epithelial mesenchymal transition(EMT)and prognosis.Methods A total of 122 pa-tients with NMIBC treated in the hospital from May 2019 to May 2021 were selected.Immunohistochemistry was used to detect the expression of NAT10 and PABPC1 proteins in NMIBC tissues.Real time fluorescence quantitative polymerase chain reaction(qPCR)was used to detect the expression of NAT10,PABPC1 mRNA,and EMT markers in NMIBC tissues.Pearson correlation analysis was conducted on the correlation between EMT indicators[Snail,N-cadherin(N-cad),vimentin(Vim)mRNA].Cox regression analysis was conducted on the relationship between NAT10,PABPC1 and prognosis of NMIBC.Results Compared with adjacent tis-sues,the expression of NAT10 mRNA,PABPC1 mRNA,Snail mRNA,N-cad mRNA,and Vim mRNA in NMIBC cancer tissues was higher,and the difference was statistically significant(P＜0.001).The expression of NAT10 mRNA,PABPC1 mRNA in NMIBC cancer tissues was positively correlated with Snail mRNA,N-cad mRNA,and Vim mRNA(r=0.678,0.702,0.711,0.754,0.788,0.663,P＜0.001).The positive rates of NAT10 and PABPC1 in NMIBC cancer tissues were 59.02％(72/122)and 60.66％(74/122),respectively,while those in adjacent tissues were 6.56％(8/122)and 4.92％(6/122),respectively(x2=76.176,85.995,P＜0.001).The positive rates of NAT10 and PABPC1 in NMIBC cancer tissues were higher than those in ad-jacent tissues,and the difference was statistically significant(x2=76.176,85.995,P＜0.001).The positivity rates of NAT10 and PABPC1 in cancer tissues of stage T1,high-grade NMIBC patients were higher than those in cancer tissues of Ta/Ti,low-grade patients,and the differences were statistically significant(P＜0.05).The 3-year overall progression free survival rates of NMIBC patients in the NAT10 positive and negative groups were 48.61％(35/72)and 80.00％(40/50),respectively,with a statistically significant difference(Log rank x2=13.780,P=0.000).The 3-year overall progression free survival rates of PABPC1 positive and negative patients were 47.30％(35/74)and 83.33％(40/48),respectively,with a statistically significant difference(Log rank x2=11.830,P=0.001).T1 stage,high-grade,NAT10 positive,and PABPC1 positive were risk fac-tors affecting the prognosis of NMIBC.Conclusion The expression of NAT10 and PABPC1 in NMIBC cancer tissue is significantly upregulated and positively correlated with EMT markers,which is correlated with poor prognosis of NMIBC.

Alcohol use disorder(AUD)is a common mental disorder and physiological disease impac-ting millions globally.Although multiple studies have explored the causes and treatments of AUD,its exact mechanisms remain poorly understood.The development of lipidomics technology provides a new perspective for studying AUD and can be used to investigate its biological mechanisms.This review summarizes recent ap-plications and progress of lipidomics in AUD research,as well as its potential value in prevention and treat-ment strategies.

[Objective] To explore the correlation between the 5-factor modified frailty index (mFI-5) and acute kidney injury (AKI) after laparoscopic radical nephrectomy in elderly patients with renal cancer, so as to provide reference for the prevention and treatment of postoperative AKI. [Methods] A retrospective analysis was conducted on the clinical data of 214 elderly patients (≥60 years) who underwent laparoscopic radical nephrectomy at our hospital during Dec.2018 and Dec.2021.Patients were divided into frail group (n=75, mFI-5≥2) and non-frail group (n=139, mFI-5<2). The incidence of AKI and sub items of mFI-5 were compared between the two groups.According to the occurrence of AKI, patients were divided into AKI group (n=77) and non-AKI group (n=137). Univariate and multivariate logistic analyses were conducted to identify risk factors of AKI.Receiver operating characteristic (ROC) curves were plotted to test the effectiveness of mFI-5 in predicting AKI. [Results] The incidence of AKI was significantly higher in the frail group than in the non-frail group (64.00% vs. 20.86%, P<0.05). Univariate analysis showed that the incidence of AKI was related to gender, diabetes, hypertension, nonfunctional independent status, weakness and split kidney glomerular filtration rate (GFR). Multivariate logistic regression analysis showed that male (OR=2.454, 95%CI: 1.193—5.047), complicated weakness (OR=6.580, 95%CI: 3.380—12.811), and low split kidney GFR (OR=0.945, 95%CI: 0.911—0.980) were independent risk factors of AKI (P<0.05). The area under the ROC curve of AKI predicted by mFI-5 was 0.711. [Conclusion] Preoperative mFI-5 score is an effective predictor of AKI in elderly patients undergoing laparoscopic radical nephrectomy.As patients with frailty have a higher risk of AKI, preoperative evaluation and monitoring should be strengthened and timely intervention should be taken to improve the prognosis.

Objective:To investigate the correlation between three-dimensional (3D) true acetabular cup coverage and two-dimensional (2D) X-ray acetabular cup coverage in total hip arthroplasty (THA) and identify effective parameters for evaluating acetabular cup coverage.Methods:Sixteen pelvic models were 3D-printed from CT reconstruction data of healthy adult pelvises. Postoperative acetabular models were prepared using standard THA procedures. The postoperative models were centered on the acetabulum and positioned under a C-arm fluoroscope. Fluoroscopy was conducted by rotating the C-arm at different angles (15°, 30°, and 45°) to obtain anteroposterior (AP) and iliac oblique X-ray images of the hip joint. The 2D acetabular coverage at different fluoroscopic angles (AP, 15° iliac oblique, 30° iliac oblique, and 45° iliac oblique) was recorded and compared with the 3D acetabular coverage measured from the pelvic models. Two independent observers assessed related parameters (coverage, abduction angle, anteversion angle) from the 2D X-ray images, and inter-observer reliability was statistically analyzed. Differences between 2D and 3D acetabular coverage were compared, and multiple linear regression analysis was used to assess correlations between acetabular anteversion angle, abduction angle, and both 2D and 3D acetabular coverage.Results:The acetabular coverage for the 2D X-ray groups (AP, 15° iliac oblique, 30° iliac oblique, and 45° iliac oblique) was 87.37%±2.59%, 87.01%±2.53%, 85.39%±2.57%, and 83.20%±2.51%, respectively, with statistically significant differences ( F=6.700, P=0.001). The 45° iliac oblique group had significantly lower coverage than both the 30° iliac oblique group and the AP group, while the 30° iliac oblique group showed significantly lower coverage than the 15° iliac oblique group (all differences, P<0.05). The mean abduction and anteversion angles were 45.33°±2.22° and 14.61°±2.53°, respectively. The 3D acetabular coverage was 85.66%±2.51%. The 45° iliac oblique group had significantly lower coverage than the 3D coverage group ( P<0.05), whereas no significant difference was observed between the AP group and the 3D coverage group ( t=1.893, P=0.062). Multiple linear regression analysis revealed significant negative correlations between the acetabular abduction angle, anteversion angle, and both 2D and 3D acetabular coverage, with the following regression equations: 2D coverage=124.627-0.700× (abduction angle) -0.379× (anteversion angle) ( R2=0.814, P=0.001); 3D coverage=120.291-0.603× (abduction angle) -0.499× (anteversion angle) ( R2=0.917, P<0.001). Conclusion:Acetabular cup coverage rates vary with different X-ray projection angles, with the anteroposterior X-ray view potentially overestimating coverage. Changes in the abduction angle appear to significantly impact the differences between 2D and 3D coverage, thereby increasing errors in assessing true acetabular cup coverage. These discrepancies between 2D and 3D coverage should be carefully considered when evaluating minimal acetabular cup coverage.

Objective:To investigate the correlation between three-dimensional (3D) true acetabular cup coverage and two-dimensional (2D) X-ray acetabular cup coverage in total hip arthroplasty (THA) and identify effective parameters for evaluating acetabular cup coverage.Methods:Sixteen pelvic models were 3D-printed from CT reconstruction data of healthy adult pelvises. Postoperative acetabular models were prepared using standard THA procedures. The postoperative models were centered on the acetabulum and positioned under a C-arm fluoroscope. Fluoroscopy was conducted by rotating the C-arm at different angles (15°, 30°, and 45°) to obtain anteroposterior (AP) and iliac oblique X-ray images of the hip joint. The 2D acetabular coverage at different fluoroscopic angles (AP, 15° iliac oblique, 30° iliac oblique, and 45° iliac oblique) was recorded and compared with the 3D acetabular coverage measured from the pelvic models. Two independent observers assessed related parameters (coverage, abduction angle, anteversion angle) from the 2D X-ray images, and inter-observer reliability was statistically analyzed. Differences between 2D and 3D acetabular coverage were compared, and multiple linear regression analysis was used to assess correlations between acetabular anteversion angle, abduction angle, and both 2D and 3D acetabular coverage.Results:The acetabular coverage for the 2D X-ray groups (AP, 15° iliac oblique, 30° iliac oblique, and 45° iliac oblique) was 87.37%±2.59%, 87.01%±2.53%, 85.39%±2.57%, and 83.20%±2.51%, respectively, with statistically significant differences ( F=6.700, P=0.001). The 45° iliac oblique group had significantly lower coverage than both the 30° iliac oblique group and the AP group, while the 30° iliac oblique group showed significantly lower coverage than the 15° iliac oblique group (all differences, P<0.05). The mean abduction and anteversion angles were 45.33°±2.22° and 14.61°±2.53°, respectively. The 3D acetabular coverage was 85.66%±2.51%. The 45° iliac oblique group had significantly lower coverage than the 3D coverage group ( P<0.05), whereas no significant difference was observed between the AP group and the 3D coverage group ( t=1.893, P=0.062). Multiple linear regression analysis revealed significant negative correlations between the acetabular abduction angle, anteversion angle, and both 2D and 3D acetabular coverage, with the following regression equations: 2D coverage=124.627-0.700× (abduction angle) -0.379× (anteversion angle) ( R2=0.814, P=0.001); 3D coverage=120.291-0.603× (abduction angle) -0.499× (anteversion angle) ( R2=0.917, P<0.001). Conclusion:Acetabular cup coverage rates vary with different X-ray projection angles, with the anteroposterior X-ray view potentially overestimating coverage. Changes in the abduction angle appear to significantly impact the differences between 2D and 3D coverage, thereby increasing errors in assessing true acetabular cup coverage. These discrepancies between 2D and 3D coverage should be carefully considered when evaluating minimal acetabular cup coverage.

In addition to respiratory symptoms, COVID-19 can also cause acute and long-term symptoms of the central nervous system, peripheral nervous system and muscular system. This article reviews the epidemiological characteristics, clinical manifestations and treatment methods of acute COVID-19 related cerebrovascular diseases, nervous system inflammatory diseases and encephalopathy, in order to provide references for clinical management of COVID-19 patients.

Objective:To explore effects of intervention based on story theory on self-management behavior and emotional state of patients with rectal cancer undergoing anus-preserving surgery.Methods:Using the convenient sampling method, a total of 80 patients with rectal cancer who underwent anus preserving surgery in Henan Provincial People's Hospital from January to September 2019 were selected as the research objects. The patients were randomly divided into 42 cases in the control group and 38 cases in the observation group. The patients in the control group adopted perioperative routine nursing methods for patients with rectal cancer, while patients in the observation group adopted an intervention program based on story theory at basis of the control group. Profile of Mood States-Short Form (POMS-SF) and Bowel Symptoms Self-Management Behaviors Questionnaire For Rectal Cancer Patients After Anus Preservation were used for evaluation.Results:In the end, the control group recovered 40 valid questionnaires and the observation group recovered 38 valid questionnaires. After intervention, scores of all dimensions of POMS-SF of the two groups were compared and the differences were statistically significant ( P<0.05) . In the observation group, scores of dimensions of stress-anxiety, depression-depression, anger-hostility, fatigue-dullness and bewilderment-disorder were lower than those of the control group, and the score of energy-vitality was higher than that of the control group. After intervention, there was no statistically significant difference in the score of treatment management dimension in Bowel Symptoms Self-Management Behaviors Questionnaire For Rectal Cancer Patients After Anus Preservation between the two groups ( P>0.05) . The scores of diet management, perianal skin management, seeking help from others and self-emotional adjustment in the observation group were higher than those in the control group, and the differences were statistically significant ( P<0.05) . Conclusions:Intervention based on story theory can effectively improve the emotional state of patients with rectal cancer undergoing anus-preserving surgery and improve dietary management, perianal skin management, seeking others' help and self-emotional regulation behaviors in patients' intestinal self-management behaviors, which has good clinical application values.

Background: In suckling piglets, transmissible gastroenteritis virus (TGEV) causes lethal diarrhea accompanied by high infection and mortality rates, leading to considerable economic losses. This study explored methods of preventing or inhibiting their production.Bovine antimicrobial peptide-13 (APB-13) has antibacterial, antiviral, and immune functions. Objectives: This study analyzed the efficacy of APB-13 against TGEV through in vivo and in vitro experiments. Methods: The effects of APB-13 toxicity and virus inhibition rate on swine testicular (ST) cells were detected using 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT). The impact of APB-13 on virus replication was examined through the 50% tissue culture infective dose (TCID50 ). The mRNA and protein levels were investigated by real-time quantitative polymerase chain reaction and western blot (WB). Tissue sections were used to detect intestinal morphological development. Results: The safe and effective concentration range of APB-13 on ST cells ranged from 0 to 62.5 µg/mL, and the highest viral inhibitory rate of APB-13 was 74.1%. The log10 TCID50 of 62.5 µg/mL APB-13 was 3.63 lower than that of the virus control. The mRNA and protein expression at 62.5 µg/mL APB-13 was significantly lower than that of the virus control at 24 hpi. Piglets in the APB-13 group showed significantly lower viral shedding than that in the virus control group, and the pathological tissue sections of the jejunum morphology revealed significant differences between the groups. Conclusions APB-13 exhibited good antiviral effects on TGEV invivo and in vitro.

Objective:To investigate the risk factors associated with anastomotic leakage after robotic surgery in mid-low rectal cancer.Methods:A retrospective case-control study method was conducted. Inclusion criteria: (1) 18 to 80 years old; (2) pathologically confirmed rectal cancer; (3) distance <10 cm from tumor to anal margin; (4) robotic anterior rectal resection. Patients with previous history of colorectal cancer surgery, distant metastases or other malignant tumors, undergoing emergency surgery, with severe abdominal adhesions or those receiving combined organ resection were excluded. Based on the above criteria, 636 patients undergoing robotic radical sphincter-preserving surgery for mid-low rectal cancer in Zhongshan Hospital from January 2015 to December 2018 were included in this study, including 398 males (62.6%) and 238 females (37.4%) with a mean age of (61.9±11.3) years. Sixty-eight cases (10.7%) received neoadjuvant chemoradiotherapy. Amony the 636 included patients, 123(19.3%) underwent natural orifice specimen extraction surgery (NOSES) and 15 (2.3%) underwent preventive stoma. According to the cirteria developed by the International Rectal Cancer Research Group in 2010, the anastomotic leakage was classified as grade A (no requirement of intervention), B (requirement of intervention), and C (requirement of operation). Logistic regression was used to analyze the relationship between anastomotic leakage and clinicopathological factors. Factors in univariate analysis with P<0.05 were included in the multivariate analysis. Results:Anastomotic leakage occurred in 38 cases (6.0%). The grading of anastomotic leakage was grade A in 13 cases (2.0%), grade B in 19 cases (3.0%), and grade C in 6 cases (0.9%). The 3-year disease-free survival rate of patients with anastomotic leakage and without anastomotic leakage was 83.5% and 83.6% respectively ( P=0.862); the 3-year overall survival rate of the two group was 85.1% and 87.5% respectively ( P=0.296). The results of univariate logistic regression analysis showed that male ( P=0.011), longer operation time ( P=0.042), distance ≤5 cm from tumor to anal margin ( P=0.012), more intraoperative blood loss ( P=0.048) were associated with anastomotic leakage (all P<0.05). NOSES was not associated with anastomotic leakage ( P=0.704). Multivariate analysis confirmed that male (OR=3.03, 95%CI: 1.37 to 7.14, P=0.010), operation time ≥180 minutes (OR=2.04, 95%CI: 1.03 to 3.99, P=0.040), distance ≤5 cm from tumor to anal margin (OR=2.56, 95%CI:1.28 to 5.26, P=0.008) were independent risk factors for anastomotic leakage. Conclusion:Male, short distance from tumor to anal margin, and long operation time are independent risk factors for anastomotic leakage in patients undergoing robotic mid-low rectal cancer radical surgeries. These patients need to be cautiously treated during surgery.

Objective:To investigate the risk factors associated with anastomotic leakage after robotic surgery in mid-low rectal cancer.Methods:A retrospective case-control study method was conducted. Inclusion criteria: (1) 18 to 80 years old; (2) pathologically confirmed rectal cancer; (3) distance <10 cm from tumor to anal margin; (4) robotic anterior rectal resection. Patients with previous history of colorectal cancer surgery, distant metastases or other malignant tumors, undergoing emergency surgery, with severe abdominal adhesions or those receiving combined organ resection were excluded. Based on the above criteria, 636 patients undergoing robotic radical sphincter-preserving surgery for mid-low rectal cancer in Zhongshan Hospital from January 2015 to December 2018 were included in this study, including 398 males (62.6%) and 238 females (37.4%) with a mean age of (61.9±11.3) years. Sixty-eight cases (10.7%) received neoadjuvant chemoradiotherapy. Amony the 636 included patients, 123(19.3%) underwent natural orifice specimen extraction surgery (NOSES) and 15 (2.3%) underwent preventive stoma. According to the cirteria developed by the International Rectal Cancer Research Group in 2010, the anastomotic leakage was classified as grade A (no requirement of intervention), B (requirement of intervention), and C (requirement of operation). Logistic regression was used to analyze the relationship between anastomotic leakage and clinicopathological factors. Factors in univariate analysis with P<0.05 were included in the multivariate analysis. Results:Anastomotic leakage occurred in 38 cases (6.0%). The grading of anastomotic leakage was grade A in 13 cases (2.0%), grade B in 19 cases (3.0%), and grade C in 6 cases (0.9%). The 3-year disease-free survival rate of patients with anastomotic leakage and without anastomotic leakage was 83.5% and 83.6% respectively ( P=0.862); the 3-year overall survival rate of the two group was 85.1% and 87.5% respectively ( P=0.296). The results of univariate logistic regression analysis showed that male ( P=0.011), longer operation time ( P=0.042), distance ≤5 cm from tumor to anal margin ( P=0.012), more intraoperative blood loss ( P=0.048) were associated with anastomotic leakage (all P<0.05). NOSES was not associated with anastomotic leakage ( P=0.704). Multivariate analysis confirmed that male (OR=3.03, 95%CI: 1.37 to 7.14, P=0.010), operation time ≥180 minutes (OR=2.04, 95%CI: 1.03 to 3.99, P=0.040), distance ≤5 cm from tumor to anal margin (OR=2.56, 95%CI:1.28 to 5.26, P=0.008) were independent risk factors for anastomotic leakage. Conclusion:Male, short distance from tumor to anal margin, and long operation time are independent risk factors for anastomotic leakage in patients undergoing robotic mid-low rectal cancer radical surgeries. These patients need to be cautiously treated during surgery.