Objective To explore the mediating role of activities of daily living (ADL) in pain and depressive symptoms in the elderly in China. Methods Utilizing the data from 2020 China Health and Retirement Longitudinal Study, 4403 Chinese elderly individuals aged ≥ 60 years old were selected as the research subjects. Depression Scale (CES-D 10) of the Center for Epidemiological Survey and ADL scale were used in the study. The PROCESS4.1 macro was used to test the mediating effect of daily living activities between pain and depressive symptoms, and the Bootstrap method was applied for verification of the mediating variables. Results A total of 2368 cases of depressive symptoms were detected in the elderly in China, with a detection rate of 53.78%. Pain was positively correlated with depressive symptoms (r=0.27, P<0.01), and activities of daily living were negatively correlated with pain and depressive symptoms (r=-0.27, -0.337, P<0.01). The results showed that the total effect value of pain on depressive symptoms was 0.33, the direct effect value was 0.24, and the mediating effect value of daily living activities was 0.09, accounting for 27.27%. Conclusion Pain and activities of daily living are important factors influencing depressive symptoms in the elderly, and activities of daily living play a partial mediating role in the relationship between pain and depressive symptoms in the elderly.

Objective:To investigate the stiffness of the gastrocnemius muscle-tendon complex at different ankle joint an-gles and its correlation with foot arch morphology in healthy subjects using shear wave elastography(SWE).Method:Forty healthy subjects were recruited and divided into standard,low-arch,and high-arch groups based on arch height index under load(AHIload).The arch stiffness index was calculated from both loaded and non-loaded arch height.The stiffness of the medial gastrocnemius,lateral gastrocnemius,and achilles tendon were measured by SWE at natural and neutral ankle positions.Result:The stiffness of medial gastrocnemius increased in the low-arch group at the neutral position compared with the high-arch group(P=0.021).The correlation coefficients between the stiffness of medial and lateral gas-trocnemius and the arch stiffness index were 0.355 and 0.484,respectively.The correlation coefficients be-tween passive ankle dorsiflexion range of motion were ﹣0.331 for the medial gastrocnemius and ﹣0.276 for the lateral gastrocnemius.The medial and lateral gastrocnemius stiffness at the natural position did not change with arch height(P>0.05).The stiffness of the achilles tendon at any ankle position showed no significant cor-relation with arch height,arch stiffness index,or passive ankle dorsiflexion range of motion(P>0.05).Conclusion:Individuals with low arches exhibited higher medial gastrocnemius stiffness.Increased arch stiff-ness and decreased passive ankle dorsiflexion were associated with higher medial gastrocnemius stiffness.

Objective:To investigate the stiffness of the gastrocnemius muscle-tendon complex at different ankle joint an-gles and its correlation with foot arch morphology in healthy subjects using shear wave elastography(SWE).Method:Forty healthy subjects were recruited and divided into standard,low-arch,and high-arch groups based on arch height index under load(AHIload).The arch stiffness index was calculated from both loaded and non-loaded arch height.The stiffness of the medial gastrocnemius,lateral gastrocnemius,and achilles tendon were measured by SWE at natural and neutral ankle positions.Result:The stiffness of medial gastrocnemius increased in the low-arch group at the neutral position compared with the high-arch group(P=0.021).The correlation coefficients between the stiffness of medial and lateral gas-trocnemius and the arch stiffness index were 0.355 and 0.484,respectively.The correlation coefficients be-tween passive ankle dorsiflexion range of motion were ﹣0.331 for the medial gastrocnemius and ﹣0.276 for the lateral gastrocnemius.The medial and lateral gastrocnemius stiffness at the natural position did not change with arch height(P>0.05).The stiffness of the achilles tendon at any ankle position showed no significant cor-relation with arch height,arch stiffness index,or passive ankle dorsiflexion range of motion(P>0.05).Conclusion:Individuals with low arches exhibited higher medial gastrocnemius stiffness.Increased arch stiff-ness and decreased passive ankle dorsiflexion were associated with higher medial gastrocnemius stiffness.

This study was aimed at exploring the prevalence and drug sensitivity of Gordonia strains isolated from sputum samples in Henan Province,to provide data to aid in the prevention and treatment of Gordonia infection.A combination of 16S rDNA and sec A1 gene sequencing was used to identify the isolated strains,and susceptibility to16 drugs was determined with the broth microdilution method.A total of 21 strains were identified through 16S rDNA gene and sec A1 gene sequencing,including five strains of Gordonia broncians,eight strains of Gordonia paraphernivans,seven strains of Gordonia sputi,and one strain of Gordonia aichiensis.Drug sensi-tivity testing showed high Gordonia sensitivity to drugs such as ceftriaxone,linezolid,doxycycline,amoxicillin/clavulanic acid,mino-cycline,cefotaxime,trimethoprim/sulfamethoxazole,imipenem,tobramycin,and clarithromycin.The sensitivity rates of the isolated strains were 90.48%(19/21),100%(21/21),90.48%(19/21),90.48%(19/21),95.24%(20/21),90.48%(19/21),90.48%(19/21),90.48%(19/21),and 95.24%(20/21),respectively.Gordonia showed high resistance to rifampicin and cefepime,with rates of 28.57%(6/21)and 19.05%(4/21),respectively.Meanwhile,the resistance varied among bacterial strains.The resistance rate of G.sputi to rifampicin reached 71.43%(5/7),whereas that of G.parapffinivoras to cefepime was 37.5%(3/8).The main species of Gordo-nia isolated from sputum samples of patients in Henan Province were G.bronchialis,G.paraffinivoras,G.sputi,and G.aichiensis.Drug sensitivity tests indicated that drugs including amoxicillin/clavulanic acid,ceftriaxone,cefotaxime,tobramycin,clarithromycin,mi-nocycline,trimethoprim/sulfamethoxazole,linezolid,and doxycycline had good antibacterial effects against Gordonia.

Aim To investigate the correlation between serum solube growth stimulation expressed gene 2 protein(sST2)and early reperfusion arrhythmia(ERA)after emergency percutaneous coronary intervention(PCI)in patients with acute ST-segment elevation myocardial infarction(STEMI).Methods A total of 202 STEMI patients who under-went emergency PCI from November 2020 to August 2022 in the Cardiac Center of Liaoning Provincial People's Hospital were divided into two groups based on the occurrence of ERA within 48 hours after PCI:ERA group and non-ERA group.Serum sST2 level and clinical data were compared between the groups.Univariable and multivariable Logistic regression analysis were used to explore the association between serum sST2 level and ERA occurrence,and restricted cubic spline model was applied to identify independent risk factors for ERA.Results There were 83(41.1％)patients experi-enced ERA within 48 hours after PCI.Compared with the non-ERA group,the patients in ERA group had shorter time from chest pain to reperfusion and higher serum sST2 level(P＜0.001).Multivariate Logistic regression analysis showed that for STEMI patients,elevated serum sST2 level(sST2≥45.03 μg/L),early reperfusion time(chest pain to successful reperfusion time≤5.23 h),high thrombosis burden,and right coronary artery as the infarct related artery(IRA)were in-dependent risk factors for ERA after emergency PCI.The restricted cubic spline model suggested that the serum sST2 lev-el of STEMI patients was nonlinearly correlated with the risk of ERA after PCI(P＜0.01),and the cutoff point was 45.12 μg/L.ROC curve analysis showed that the area under the ROC curve of serum sST2 level in predicting ERA oc-currence after PCI was 0.827(95％CI:0.771～0.883).Conclusion The high serum sST2 level before PCI is an independent risk factor for ERA occurrence after PCI in patients with STEMI.When serum sST2＞45.12 μg/L,its level is positively correlated with the risk of ERA.

Objective:To establish an element framework and template of broad informed consent applicable to clinical research,and to standardize the collection,storage,and reuse of medical data and biological samples,making them comply with ethical and legal requirements.Methods:A literature review and group discussion were employed to construct the draft of the element framework and template of broad informed consent form.The Delphi expert consultation method was used to conduct two rounds of correspondence with 13 experts in relevant fields to determine the two-level element framework and template of broad informed consent form.Results:The response rates for the two rounds of expert consultation questionnaires were above 90%,the experts'positive coefficients were good,and the coefficients of authority(Cr)were higher than 0.85.In the second round of consultation,the average importance value was≥4.4,the coefficient of variation(CV)was<0.17,and Kendall's W was 0.184(P<0.001),indicating that the expert opinions tended to be consistent.Ultimately,an element framework and template of broad informed consent form was established,consisting of 4 first-level items and 21 second-level items.Conclusion:The constructed element framework and template of broad informed consent form is highly scientific and applicable,providing references for clinical research.

Aim To investigate the correlation between serum solube growth stimulation expressed gene 2 protein(sST2)and early reperfusion arrhythmia(ERA)after emergency percutaneous coronary intervention(PCI)in patients with acute ST-segment elevation myocardial infarction(STEMI).Methods A total of 202 STEMI patients who under-went emergency PCI from November 2020 to August 2022 in the Cardiac Center of Liaoning Provincial People's Hospital were divided into two groups based on the occurrence of ERA within 48 hours after PCI:ERA group and non-ERA group.Serum sST2 level and clinical data were compared between the groups.Univariable and multivariable Logistic regression analysis were used to explore the association between serum sST2 level and ERA occurrence,and restricted cubic spline model was applied to identify independent risk factors for ERA.Results There were 83(41.1％)patients experi-enced ERA within 48 hours after PCI.Compared with the non-ERA group,the patients in ERA group had shorter time from chest pain to reperfusion and higher serum sST2 level(P＜0.001).Multivariate Logistic regression analysis showed that for STEMI patients,elevated serum sST2 level(sST2≥45.03 μg/L),early reperfusion time(chest pain to successful reperfusion time≤5.23 h),high thrombosis burden,and right coronary artery as the infarct related artery(IRA)were in-dependent risk factors for ERA after emergency PCI.The restricted cubic spline model suggested that the serum sST2 lev-el of STEMI patients was nonlinearly correlated with the risk of ERA after PCI(P＜0.01),and the cutoff point was 45.12 μg/L.ROC curve analysis showed that the area under the ROC curve of serum sST2 level in predicting ERA oc-currence after PCI was 0.827(95％CI:0.771～0.883).Conclusion The high serum sST2 level before PCI is an independent risk factor for ERA occurrence after PCI in patients with STEMI.When serum sST2＞45.12 μg/L,its level is positively correlated with the risk of ERA.

This study was aimed at exploring the prevalence and drug sensitivity of Gordonia strains isolated from sputum samples in Henan Province,to provide data to aid in the prevention and treatment of Gordonia infection.A combination of 16S rDNA and sec A1 gene sequencing was used to identify the isolated strains,and susceptibility to16 drugs was determined with the broth microdilution method.A total of 21 strains were identified through 16S rDNA gene and sec A1 gene sequencing,including five strains of Gordonia broncians,eight strains of Gordonia paraphernivans,seven strains of Gordonia sputi,and one strain of Gordonia aichiensis.Drug sensi-tivity testing showed high Gordonia sensitivity to drugs such as ceftriaxone,linezolid,doxycycline,amoxicillin/clavulanic acid,mino-cycline,cefotaxime,trimethoprim/sulfamethoxazole,imipenem,tobramycin,and clarithromycin.The sensitivity rates of the isolated strains were 90.48%(19/21),100%(21/21),90.48%(19/21),90.48%(19/21),95.24%(20/21),90.48%(19/21),90.48%(19/21),90.48%(19/21),and 95.24%(20/21),respectively.Gordonia showed high resistance to rifampicin and cefepime,with rates of 28.57%(6/21)and 19.05%(4/21),respectively.Meanwhile,the resistance varied among bacterial strains.The resistance rate of G.sputi to rifampicin reached 71.43%(5/7),whereas that of G.parapffinivoras to cefepime was 37.5%(3/8).The main species of Gordo-nia isolated from sputum samples of patients in Henan Province were G.bronchialis,G.paraffinivoras,G.sputi,and G.aichiensis.Drug sensitivity tests indicated that drugs including amoxicillin/clavulanic acid,ceftriaxone,cefotaxime,tobramycin,clarithromycin,mi-nocycline,trimethoprim/sulfamethoxazole,linezolid,and doxycycline had good antibacterial effects against Gordonia.

Objective:To establish an element framework and template of broad informed consent applicable to clinical research,and to standardize the collection,storage,and reuse of medical data and biological samples,making them comply with ethical and legal requirements.Methods:A literature review and group discussion were employed to construct the draft of the element framework and template of broad informed consent form.The Delphi expert consultation method was used to conduct two rounds of correspondence with 13 experts in relevant fields to determine the two-level element framework and template of broad informed consent form.Results:The response rates for the two rounds of expert consultation questionnaires were above 90%,the experts'positive coefficients were good,and the coefficients of authority(Cr)were higher than 0.85.In the second round of consultation,the average importance value was≥4.4,the coefficient of variation(CV)was<0.17,and Kendall's W was 0.184(P<0.001),indicating that the expert opinions tended to be consistent.Ultimately,an element framework and template of broad informed consent form was established,consisting of 4 first-level items and 21 second-level items.Conclusion:The constructed element framework and template of broad informed consent form is highly scientific and applicable,providing references for clinical research.

Objective　To understand the typing and drug sensitivity results of M. kansasii strains in He'nan Province, and to provide basic data for the prevention and treatment of M. kansasii infections. Methods　Positive cultures preliminarily identified as nontuberculous mycobacteria from designated tuberculosis medical institutions across He'nan Province from 2019 to 2022 were collected for strain identification. The minimum inhibitory concentration (MIC) of 11 drugs of 34 clinical isolates of M. kansasii was determined by the microdilution method. PCR-restriction enzyme pattern analysis was used for typing. Results　As a result, 34 clinical isolates of M. kansasii were totally sensitive to three drugs: amikacin, linezolid, and rifampicin. The MIC50 and MIC90 of amikacin, linezolid, and rifampicin were 2 mg/L and 4 mg/L, 1 mg/L and 1 mg/L, and 0.25 mg/L and 0.25 mg/L, respectively. The resistance rates to clarithromycin, rifampicin, minocycline, moxifloxacin, and trimethoprim/sulfamethoxazole were 2.94%, 8.82%, 2.94%, 5.88%, and 11.76%, respectively. The MIC50 and MIC90 of clarithromycin, rifampicin, minocycline, moxifloxacin, and trimethoprim/sulfamethoxazole were 1 mg/L and 1 mg/L, 0.5 mg/L and 1 mg/L, 1 mg/L and 2 mg/L, 0.25 mg/L and 0.25 mg/L, as well as 0.25/4.75 mg/L and 8/152 mg/L, respectively. The clinical isolates of M. kansasii showed relatively high resistance rates to doxycycline, ethambutol, and ciprofloxacin, with resistance rates of 94.12%, 50.00%, and 44.12%, respectively. Thirty-four strains of M. kansasii showed varying degrees of resistance to 11 antibiotics, while there were a total of 25 strains (73.53%) resistant to more than two drugs and a total of 4 strains (11.76%) resistant to more than four drugs. According to PCR-restriction enzyme pattern analysis, 34 strains of M. kansasii belong to subtype I. Conclusions　Clarithromycin, rifampicin, amikacin, linezolid, minocycline, moxifloxacin, rifampicin, and trimethoprim/sulfamethoxazole have good in vitro antibacterial activity against clinical strains of M. kansasii isolated from He'nan Province mainly consisted of subtype I.