In order to investigate the clinical manifestations and laboratory findings associated with brucellosis for the purpose of facilitating prompt clinical diagnosis and effective treatment, as well as to offer a laboratory reference for the prevention of brucellosis outbreaks. In this study, a retrospective cohort design was employed to gather epidemiological characteristics, clinical symptoms, and associated laboratory data from 391 patients diagnosed with bacterial culture-positive brucellosis at the People′s Hospital of Ningxia Hui Autonomous Region between 2019 and 2023. The patients were categorized into four age groups, with each group representing a 20 years age interval. Statistical analyses were conducted to examine the differences between these age groups using R version 4.3.2 and SPSS version 24.0 software. The results showed that among the 391 cases of brucellosis patients, fever (241 cases, 61.64%), lumbar and leg joint pain (225 cases, 57.54%), abnormal liver function (124 cases, 31.71%), spleen enlargement (78 cases, 19.95%), and cholecystitis (75 cases, 19.18%) were identified as the most prevalent clinical symptoms. Furthermore, the presence of clinical symptoms such as fever, lumbar and leg joint pain, abdominal pain and distension, headache, dizziness, rash, and complications including abnormal liver function, cholecystitis, spleen enlargement, exhibited statistical significance across four distinct age groups ( P<0.05). The initial presentation of acute fever and rash was more pronounced in the pediatric cohort. In contrast, the youth group exhibited a higher prevalence of acute fever, liver function abnormalities, dizziness, headache, and splenomegaly. Furthermore, the strong-age group and the older age group more frequently experienced lumbar and leg joint pain, and symptoms related to the liver, gallbladder, spleen, and digestive system. In terms of laboratory examination, the rates of decreased white blood cell count (WBC), red blood cell count (RBC) and platelet count (PLT) were 18.91% (73 cases), 28.94% (112 cases) and 22.34% (86 cases), respectively. C-reactive protein (CRP), alanine aminotransferase (ALT), aspartate aminotransferase (AST), total biluric acid (TBA), adenosine deaminase (ADA), alkaline phosphatase (ALP), glutamyl transpeptidase (GGT), procalcitonin (PCT), and D-Dimer (D-Dimer). The proportion of patients with elevated quantitative test results was 88.56% (271 cases), 50.90% (197 cases), 52.20% (202 cases), 23.82% (91 cases), 75.72% (290 cases), 23.06% (89 cases), 40.89% (157 cases), 68.34% (218 cases) and 73.33% (209 cases), respectively. Among them, the quantitative detection results of CPR, ALP and D-Dimer had statistical significance among the 4 age groups ( χ2=16.366,28.089,7.880, P<0.05). Abnormally elevated laboratory parameters, including C-reactive protein (CRP), alkaline phosphatase (ALP), and D-dimer, were more pronounced in middle-aged and older cohorts, with a positive correlation to advancing age. Among the 391 brucella culture positive specimens, the top three departments with the highest number of isolations were infectious disease (52.4%, 204 cases), emergency (9.5%, 37 cases) and orthopedics (8.4%, 33 cases). In conclusion, the clinical manifestations of brucellosis are multifaceted and heterogeneous, frequently involving multiple organ systems, and the laboratory test results exhibit considerable variability. These findings indicate that clinicians should integrate clinical features of brucellosis with laboratory test results, considering the patient′s age group, to enhance diagnostic accuracy and triage efficiency in suspected cases. Improving relevant diagnostic examinations can thereby aid in the effective control and prevention of the disease.

In order to investigate the clinical manifestations and laboratory findings associated with brucellosis for the purpose of facilitating prompt clinical diagnosis and effective treatment, as well as to offer a laboratory reference for the prevention of brucellosis outbreaks. In this study, a retrospective cohort design was employed to gather epidemiological characteristics, clinical symptoms, and associated laboratory data from 391 patients diagnosed with bacterial culture-positive brucellosis at the People′s Hospital of Ningxia Hui Autonomous Region between 2019 and 2023. The patients were categorized into four age groups, with each group representing a 20 years age interval. Statistical analyses were conducted to examine the differences between these age groups using R version 4.3.2 and SPSS version 24.0 software. The results showed that among the 391 cases of brucellosis patients, fever (241 cases, 61.64%), lumbar and leg joint pain (225 cases, 57.54%), abnormal liver function (124 cases, 31.71%), spleen enlargement (78 cases, 19.95%), and cholecystitis (75 cases, 19.18%) were identified as the most prevalent clinical symptoms. Furthermore, the presence of clinical symptoms such as fever, lumbar and leg joint pain, abdominal pain and distension, headache, dizziness, rash, and complications including abnormal liver function, cholecystitis, spleen enlargement, exhibited statistical significance across four distinct age groups ( P<0.05). The initial presentation of acute fever and rash was more pronounced in the pediatric cohort. In contrast, the youth group exhibited a higher prevalence of acute fever, liver function abnormalities, dizziness, headache, and splenomegaly. Furthermore, the strong-age group and the older age group more frequently experienced lumbar and leg joint pain, and symptoms related to the liver, gallbladder, spleen, and digestive system. In terms of laboratory examination, the rates of decreased white blood cell count (WBC), red blood cell count (RBC) and platelet count (PLT) were 18.91% (73 cases), 28.94% (112 cases) and 22.34% (86 cases), respectively. C-reactive protein (CRP), alanine aminotransferase (ALT), aspartate aminotransferase (AST), total biluric acid (TBA), adenosine deaminase (ADA), alkaline phosphatase (ALP), glutamyl transpeptidase (GGT), procalcitonin (PCT), and D-Dimer (D-Dimer). The proportion of patients with elevated quantitative test results was 88.56% (271 cases), 50.90% (197 cases), 52.20% (202 cases), 23.82% (91 cases), 75.72% (290 cases), 23.06% (89 cases), 40.89% (157 cases), 68.34% (218 cases) and 73.33% (209 cases), respectively. Among them, the quantitative detection results of CPR, ALP and D-Dimer had statistical significance among the 4 age groups ( χ2=16.366,28.089,7.880, P<0.05). Abnormally elevated laboratory parameters, including C-reactive protein (CRP), alkaline phosphatase (ALP), and D-dimer, were more pronounced in middle-aged and older cohorts, with a positive correlation to advancing age. Among the 391 brucella culture positive specimens, the top three departments with the highest number of isolations were infectious disease (52.4%, 204 cases), emergency (9.5%, 37 cases) and orthopedics (8.4%, 33 cases). In conclusion, the clinical manifestations of brucellosis are multifaceted and heterogeneous, frequently involving multiple organ systems, and the laboratory test results exhibit considerable variability. These findings indicate that clinicians should integrate clinical features of brucellosis with laboratory test results, considering the patient′s age group, to enhance diagnostic accuracy and triage efficiency in suspected cases. Improving relevant diagnostic examinations can thereby aid in the effective control and prevention of the disease.

Objective To compare and analyse the detection performance of different 2019-new coronavirus (2019-nCoV) nucleic acid detection kits, in order to provide references for laboratory. Methods Six kinds of domestic reagents (A—F reagent) were selected for parallel detection of a series of samples from one patient in this hospital whose 2019-nCoV nucleic acid result was confirmed weakly positive. The samples were taken at three different times, the RNAs were extracted and amplified, and two parallel tests were performed each time by use of these six kits. The detection performance was compared according to the results of each kit. Results The three parallel test results (ORF1ab and N gene) of C and F reagents were positive, the results of D reagent showed the N gene was not detected, and the results of A, B, E reagents showed the ORF1ab gene was not detected sometimes. The reproducibility of in-batch detections by C reagent was the best, and the CT values of F reagents (N and ORF1ab), E reagents (ORF1ab) and A reagents (ORF1ab) showed changes in trend. Conclusion There are differences in the detection ability of six 2019-nCoV nucleic acid detection reagents for weakly positive samples, and the accuracy, sensitivity and reproducibility of some reagents are not good. There is an urgent need to further optimize and improve their performance in order to better meet the needs of large-scale screening.

ObjectiveToinvestigatetheeffectandmechanismofaChinesetraditionalprescription,YuebiBanxia decoction, on allergic asthma in mice by observing the changes of serum immunoglobulin E ( IgE ) and lung tissue superoxide dismutase ( SOD) activity in the mouse model of allergic asthma .Methods Forty healthy Kunming mice were randomly divided into normal control group , asthma model group , dexamethasone intervention group and Chinese medicine preparation group .The mouse model of asthma was generated by peritoneal injection of ovalbumin ( OVA) and suspension of aluminium hydroxide .The mice of traditional Chinese medicine group received Yuebi Banxia decoction in a dose of 1 mL/100 g, daily for 30 days.The mice of dexamethasone group were given intraperitoneal injection of dexamethasone 1 mg/kg.The normal control group and asthma model group were given gastric gavage of physiological saline.After 30 days administration, the serum levels of IgE and SOD activity of lung tissue were determined , and the pathological changes of lung tissue were observed using HE staining .Results The mice of traditional Chinese medicine preparation group showed significantly decreased serum IgE level , and significantly increased SOD activity than those of the asthma model group mice (P<0.05 for both).Conclusions Reduced serum IgE and increased SOD activity in the lung tissue of mice with allergic asthma , improving the antioxidant ability of lung tissue , may be involved in the anti-allergic asthma mechanism of the Chinese traditional prescription Yuebi Banxia decoction .