Objective: To investigate the correlation between color parameters (L^*, a^*, b^*, Eab^*) and the contents of reducing sugars, total polysaccharides, total oligosaccharides, as well as four saccharides (fructose, glucose, sucrose, and kestose) during the nine cycles of steaming and sun-drying processing of Polygonatum kingianum, and to preliminarily explore the optimal processing duration.
Methods: The color changes were objectively evaluated using a colorimeter. The anthrone-sulfuric acid method was employed to determine total polysaccharides and oligosaccharides. The 3,5-dinitrosalicylic acid (DNS) colorimetric method was used to measure total reducing sugar content. High-performance liquid chromatography coupled with charged aerosol detection (HPLC-CAD) was applied for quantitative analysis of fructose, glucose, sucrose, and kestose. Multivariate statistical analysis was conducted to assess samples from different processing stages.
Results: Significant variations in color and component contents were observed across processing stages. The herbal pieces progressively darkened with increased processing cycles: brightness (L^*) and total color difference (Eab^*) initially decreased then stabilized, while a^* (red-green) and b^* (yellow-blue) values first increased then declined. Total polysaccharides and oligosaccharides showed overall decreasing trends, whereas reducing sugars initially increased before stabilizing. Fructose and glucose levels rose continuously, while sucrose and kestose decreased progressively, becoming undetectable after five cycles.
Conclusion: The chromatic alteration and saccharide composition of P. kingianum showed significant correlation with processing duration. Both total color difference (Eab^*) and reducing sugar content stabilized after four processing cycles (12 hours), suggesting that four cycles of steaming and sun-drying may represent the optimal processing duration.

Objective: To investigate the correlation between the color parameters (L^*, a^*, b^*, Eab^* values) of Polygoni Multiflori Radix Praeparata powder prepared by different processing techniques and the contents of 2,3,5,4’-tetrahydroxystilbene-2-O-β-D-glucopyranoside, emodin, physcion, emodin-8-O-β-D-glucopyranoside, physcion-8-O-β-D-glucopyranoside.
Methods: The L^*, a^*, b^* and Eab^* values of Polygoni Multiflori Radix Praeparata powder prepared by different processing techniques were determined by spectrophotometer, and the contents of the five components were determined by high performance liquid chromatography. Secondly, SPSS 26.0 software and Simca 14.1 software were used to analyze the correlation.
Results: Through the hierarchical cluster analysis (HCA), it was found that the steamed samples and black bean steamed samples could be obviously divided into two categories: raw products and processed products. The processed products could be further divided into 2-8 h and 12-48 h. Pearson correlation analysis showed that the content of stilbene glycoside was significantly positively correlated with L^*, a^* and b^* values (P<0.01). The a^* value was significantly positively correlated with the content of emodin and physcion (P<0.01). Emodin-8-O-β-D-glucoside was positively correlated with L^* value and b^* value, while physcion-8-O-β-D-glucoside was negatively correlated with a^* value. Partial least squares discriminant analysis (PLS) showed that 2,3,5,4’-tetrahydroxystilbene-2-O-β-D-glucoside (VIP=1.69) and emodin-8-O-β-D-glucoside (VIP=1.06) were the key variables affecting chromaticity characteristics (P<0.01).
Conclusion: The three processes of steaming, black bean steaming and black bean stewing are consistent in composition transformation and chromaticity variation, and stilbene glycoside can be used as a specific index component to characterize the processed color. Chromatic parameters can effectively reflect the processing progression and serve as quality monitoring indicators during production.

OBJECTIVES: Tiletamine, a veterinary anesthetic, has emerged as a novel psychoactive substance and has been abused in many parts of the world, causing great harm to public health. However, the sensitivity of existing detection methods cannot meet the needs of forensic practice. This study aims to establish an ultra-high-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method for the determination of tiletamine and its metabolite desethyltiletamine in human biological samples, and to verify its applicability in forensic practice. METHODS: SKF_525A was used as the internal standard. Biological samples were extracted with acetonitrile containing 1 ng/mL SKF_525A, vortexed for 10 min, ultrasonicated for 20 min, centrifuged at 10 000 r/min for 10 min, and 500 μL of the supernatant was filtered through a 0.22 μm membrane. Analyses were performed using an ACQUITY UPLC H-Class PLUS system and an XEVO TQ-S Micro triple quadrupole mass spectrometer. An ACQUITY UPLC^® BEH C18 (1.7 µm, 2.1 mm×100 mm) column at a flow rate of 0.3 mL/min was used, and four mobile phase systems were tested to optimize separation. Detection used positive electrospray ionization (ESI+) in multiple reaction monitoring (MRM) mode, with quantifier ion transitions of mass to charge 224.043→179.016 for tiletamine and mass to charge 196.08→151.06 for desethyltiletamine. Calibration curves were established over 0.1-200 ng/mL in spiked blood samples. The linear range, limit of detection (LOD), and limit of quantification (LOQ) were determined. Low (5 ng/mL), medium (20 ng/mL), and high (100 ng/mL) concentrations of tiletamine were spiked into blood, liver, and kidney to evaluate precision, accuracy, matrix effect, recovery, and stability. Finally, actual forensic case samples were tested to validate applicability. RESULTS: The established UPLC-MS/MS method achieved simultaneous detection of tiletamine and desethyltiletamine in human biological samples, with retention times of 3.42 min and 2.82 min, respectively. Using mobile phase A (20 mmol/L ammonium acetate and 0.1% formic acid in water) and mobile phase B (acetonitrile) produced the best separation. In blood, tiletamine showed good linearity from 0.1-200 ng/mL (r=0.992, R²=0.983), LOD 0.03 ng/mL, LOQ 0.1 ng/mL, recovery 92%-107%, and matrix effect 71%-99%. In liver and kidney, recoveries were 91%-98% and 93%-104%, and matrix effects were 69%-96% and 72%-100%, respectively. Intra- and inter-day precision [expressed as relative standard deviation (RSD)] and accuracy [expressed as relative error (RE)] were within 15%, and samples were stable at -20 ℃. Tiletamine was detected in actual case samples at 0.37 μg/mL (blood), 0.15 μg/g (liver), 0.11 μg/g (kidney) in case 1, and 8.75 ng/mL (blood) in case 2; desethyltiletamine was also detected in blood. CONCLUSIONS The UPLC-MS/MS method is efficient, accurate, and sensitive, and is suitable for detecting tiletamine and desethyltiletamine in human biological samples.
Tandem Mass Spectrometry/methods* ; Humans ; Chromatography, High Pressure Liquid/methods* ; Substance Abuse Detection/methods* ; Liquid Chromatography-Mass Spectrometry

OBJECTIVES: Near vision loss (NVL) is one of the leading causes of visual impairment worldwide, exerting a profound impact on individual quality of life and socio-economic development. This study aims to analyze the burden of NVL in China by sex and age groups from 1990 to 2021 and to project trends over the next 15 years. METHODS: Using data from the Global Burden of Disease (GBD) 2021 database, we conducted descriptive analyses of NVL prevalence in China, calculated age-standardized prevalence rates (ASPR) and age-standardized disability-adjusted life years rates (ASDR) to compare burden differences between sexes and age groups, and applied an autoregressive integrated moving average (ARIMA) model to predict NVL trends for the next 15 years. The model selection was based on best-fit criteria to ensure reliable projections. RESULTS: From 1990 to 2021, China's ASPR of NVL rose from 10 096.24/100 000 to 15 624.54/100 000, and ASDR increased from 101.75/100 000 to 158.75/100 000. In 2021, ASPR (16 551.70/100 000) and ASDR (167.69/100 000) were higher among females than males (14 686.21/100 000 and 149.76/100 000, respectively). China ranked highest globally in both NVL cases and disability-adjusted life years (DALYs), with female burden significantly exceeding male burden. Projections indicated this trend and sex gap will persist until 2036. Compared with 1990, the prevalence cases and DALYs increased by 239.20% and 238.82%, respectively in 2021, with the highest burden among females and the 55-59 age group. The ARIMA model predicted continued increases in prevalence and DALYs by 2036, with females maintaining a higher burden than males. CONCLUSIONS This study reveals a marked increase in the NVL burden in China and predicts continued growth in the coming years. Public health policies should prioritize NVL prevention and control, with special attention to women and middle-aged populations to mitigate long-term societal and health impacts.
Humans ; China/epidemiology* ; Male ; Female ; Prevalence ; Middle Aged ; Disability-Adjusted Life Years ; Adult ; Global Burden of Disease ; Adolescent ; Quality-Adjusted Life Years ; Aged ; Sex Factors ; Child ; Young Adult ; Child, Preschool ; Cost of Illness ; Infant ; Aged, 80 and over ; Vision Disorders/epidemiology*

OBJECTIVES: Stress urinary incontinence (SUI) is a common condition among women that severely impairs quality of life. Pelvic floor proprioceptive training (PFPT) has attracted increasing attention for its potential to enhance pelvic floor muscle function and alleviate SUI symptoms. This study aims to observe and compare the clinical efficacy of PFPT combined with electroacupuncture, electrical stimulation, and biofeedback therapy versus conventional therapy consisting of electroacupuncture, electrical stimulation, and biofeedback alone in women with SUI, and to explore the role of PFPT in improving symptom and functional outcomes. METHODS: In this randomized controlled trial, 72 women with mild to moderate SUI were recruited from the Department of Rehabilitation Medicine at Third Xiangya Hospital, Central South University, between December 2021 and October 2023. Participants were randomly assigned to an experimental group (n=36) or a control group (n=36). Both groups received health education. The control group underwent electroacupuncture combined with electrical stimulation and biofeedback therapy, while the experimental group additionally received PFPT 3 times per week for 4 weeks. The primary outcome was assessed using the International Consultation on Incontinence Questionnaire-Short Form (ICIQ-SF). Secondary outcomes included pelvic floor muscle strength, bladder neck mobility, and balance ability. The ICIQ-SF was reassessed at 1, 3, 6, and 12 months post-treatment. RESULTS: Both groups showed statistically significant improvements in all parameters after treatment (all P<0.05). However, there were no statistically significant differences between groups in most measures (all P>0.05). The experimental group demonstrated longer single-leg stance duration with eyes closed than the control group (left leg: P=0.026; right leg: P=0.006), with a significant increase from baseline (P<0.001). At 6 months post-treatment, the cure rate in the experimental group was significantly higher than that in the control group (P=0.037). CONCLUSIONS Conventional therapy effectively improves SUI symptoms, but adding PFPT provides notable additional benefits, including enhanced balance ability and sustained mid-term cure rates. These findings suggest that PFPT is a valuable adjunct to standard SUI management strategies.
Humans ; Female ; Urinary Incontinence, Stress/physiopathology* ; Pelvic Floor/physiopathology* ; Middle Aged ; Biofeedback, Psychology ; Adult ; Exercise Therapy/methods* ; Proprioception ; Electroacupuncture/methods* ; Quality of Life ; Electric Stimulation Therapy/methods* ; Treatment Outcome ; Combined Modality Therapy

Objective:To explore the effect of bone morphogenetic with protein 2 (BMP2) intervention in iron metabolism related protein expression on rats osteoporosis (OP) by regulating hepcidin level.Methods:Thirty-six 8-week-old SPF grade female SD rats with a body weight of 200-220 g were divided into sham group (Removal of adipose tissue around ovaries), model group (ovariectomy was used to prepare postmenopausal OP model rats), adenovirus group (OP model rats injected with adenovirus into the femoral cavity), and overexpression BMP2 adenovirus group (OP model rats injected with BMP2 overexpressing adenovirus into the femoral cavity) using a random number table method, with 9 rats in each group. Rats were euthanized after 2 months of injection, and femoral tissue was taken for micro computed tomography (micro-CT) three-dimensional reconstruction to observe the microstructural characteristics of bone trabeculae, and calculate bone mineral density (BMD), trabecular number (Tb.N), trabecular thickness (Tb.Th), bone volume/total volume (BV/TV), and trabecular separation (Tb.Sp) values. Hematoxylin eosin staining, Masson staining, and tartrate-resistant acid phosphatase (TRAP) staining were used to observe the pathological damage, collagen content, and osteoclast count of femoral tissues in four group rats. Malondialdehyde (MDA), glutathione (GSH), superoxide dismutase (SOD), ferritin, hepcidin levels in serum were detected. Real-time quantitative PCR was used to detect BMP2 mRNA expression in femoral tissue. Western blotting were used to detect BMP2, ALP, OCN, OPN, RANKL, TRAP, CTX-I, hepcidin, FPN1, TfR1, DMT1 protein expression in femoral tissue.Results:The differences of BMD, Tb.N, Tb.Th, BV/TV, and Tb.Sp in sham group, model group, adenovirus group, and BMP2 overexpressing adenovirus group were statistically significant ( P<0.05), among them, BMD, Tb.N, Tb.Th, and BV/TV in model group were lower than those in sham group, Tb.Sp was higher than that in sham group, BMD, Tb.N, Tb.Th, and BV/TV in BMP2 overexpressing adenovirus group were higher than those in model group and adenovirus group, Tb.Sp was lower than that in model group and adenovirus group ( P<0.05). Sham group had intact bone trabeculae, abundant collagen fibers, and fewer osteoclasts in femoral tissues; Model group and adenovirus group had sparse bone trabeculae, severe loss of collagen fibers, and more osteoclasts; Bone trabeculae and collagen fibers in overexpression BMP2 adenovirus group were greater than those in model group and adenovirus group, osteoclasts were fewer than those in model group and adenovirus group. The differences of MDA, GSH, SOD, ferritin, and hepcidin in serum in four groups were statistically significant ( P<0.05), among them, MDA and ferritin in model group were higher than those in sham group, GSH, SOD, hepcidin were lower those in sham group, MDA and ferritin levels in overexpression BMP2 adenovirus group were lower those in model group and adenovirus group, GSH, SOD, hepcidin were higher than those in model group and adenovirus group ( P<0.05). BMP2 mRNA expression of femoral tissues in four groups were 1.00±0.10, 0.39±0.12, 0.39±0.08, and 3.46±0.41, respectively, BMP2 protein expression were 0.61±0.06, 0.17±0.06, 0.20±0.05, and 1.03±0.07, respectively, the differences were statistically significant ( P<0.05), among them, model group were lower those in sham group, overexpression BMP2 adenovirus group were higher than those in model group and adenovirus group ( P<0.05). The differences of ALP, OCN, OPN, RANKL, TRAP, CTX-I, hepcidin, FPN1, TfR1, and DMT1 expression of femoral tissues in sham group, model group, adenovirus group, and BMP2 overexpressing adenovirus group were statistically significant ( P<0.05), among them, ALP, OCN, OPN, and hepcidin in model group were lower than those in sham group, RANKL, TRAP, CTX-I, FPN1, TfR1, and DMT1 were higher than those in sham group, ALP, OCN, OPN, and hepcidin in BMP2 overexpressing adenovirus group were higher than those in model group and adenovirus group, RANKL, TRAP, CTX-I, FPN1, TfR1, and DMT1 were lower than those in model group and adenovirus group ( P<0.05). Conclusion:BMP2 intervention iron metabolism related protein expression promotes bone formation in OP rats by increasing hepcidin level.

Objective To investigate the proportion of Th17 cells,Th1-like Th17 cells and FoxP3+Th17 cells in bone marrow of patients with myelodysplastic syndrome(MDS),the expression of interleukin-17A(IL-17A)in bone marrow supernatant and its clinical significance.Methods Forty MDS patients(MDS group)and 18 patients with nutritional anemia(control group)were selected.MDS patients were classified into the low blast(MDS-LB)group(19 cases)and the increased blast(MDS-IB)group(21 cases,including 11 cases of type IB1 and 10 cases of type IB2)based on morphological definition.The MDS patients were scored according to the revised International Prognostic Scoring System(IPSS-R),with 18 cases in the low-risk group(≤4.5)and 22 cases in the high-risk group(>4.5).Flow cytometry was used to detect the proportion of Th17 cells,Th1-like Th17 cells and FoxP3+Th17 cells in bone marrow of the MDS group and the control group.Enzyme-linked immunosorbent assay(ELISA)was used to detect the level of IL-17A in bone marrow supernatant of the above samples.Results The proportion of Th17 cells and the level of IL-17A were higher in patients of the MDS group than those in the control group(P＜0.05).According to the median expression level of IL-17A,the MDS group was divided into the low-expression group(＜13.71 ng/L,20 cases)and the high-expression group(≥13.71 ng/L,20 cases).Compared with the low-expression group,there were higher proportion of patients with blast cells＜5%and low-risk patients(P＜0.05)in the high-expression group.Compared with the IL-17A low-expression group,the IL-17A high-expression group had a higher proportion of patients with blast cells＜5%and relatively low-risk patients(P＜0.05).Compared with the low-risk patients,high-risk patients had a lower proportion of Th17 cells,IL-17A levels and Th1-like Th17 cells,and a higher proportion of FoxP3+Th17 cells(P＜0.05).Compared with the MDS-LB group,the MDS-IB group had a lower proportion of Th17 cells,IL-17A levels and Th1-like Th17 cells,and a higher proportion of FoxP3+Th17 cells(P＜0.05).Conclusion The proportion of Th17 cells and the level of IL-17A are significantly increased in MDS patients.The decreased proportion of Th1-like Th17 cells and the increased proportion of FoxP3+Th17 cells may be related to the increased proportion of blast cells and higher risk stratification in patients.

Objective To evaluate the application of rabbit liver cancer model in teaching interventional medicine for graduate students.Methods A total of 10 first-year master graduate students majoring in Radiological Imaging(Interventional Medicine).who were studying at Zhengzhou University of China,were enrolled in this study.The rabbit liver cancer model was used as the experimental teaching materials.The teaching contents included the establishment of rabbit liver cancer model,the interventional operation of rabbit liver cancer,the method of scientific research and teaching,the evaluation of the teaching effect,and the survey of student satisfaction.Results Under the guidance of teaching tutor,the success rate of VX2 rabbit liver cancer modeling performed by the 10 master graduate students majoring in interventional medicine was 100％,and the mean operational quality assessment score was(11.5±2.0)points.During the operation of interventional surgery,the success rate of femoral artery puncture was also 100％,and the mean score for each interventional operation was(11.8±2.3)points.The students'experimental designs were evaluated by the expert group,the results were as follows:2 cases were rated as excellent,7 cases were rated as good,and one case was rated as moderate.The degree of students'satisfaction with experimental teaching method was high,the specific scores of each item are as follows:the understanding of the rabbit liver cancer model was(4.80±0.40)points,the command of interventional technology was(4.60±0.49)points,and the quality and practicability of teaching materials was(4.90±0.30)points.Conclusion This teaching method of using rabbit liver cancer model experiment can improve the animal experiment ability,interventional operation ability and scientific research innovation ability of graduate students.Animal model teaching method is an innovation of teaching mode for graduate students majoring in interventional medicine.

Objective:To explore the effect of bone morphogenetic with protein 2 (BMP2) intervention in iron metabolism related protein expression on rats osteoporosis (OP) by regulating hepcidin level.Methods:Thirty-six 8-week-old SPF grade female SD rats with a body weight of 200-220 g were divided into sham group (Removal of adipose tissue around ovaries), model group (ovariectomy was used to prepare postmenopausal OP model rats), adenovirus group (OP model rats injected with adenovirus into the femoral cavity), and overexpression BMP2 adenovirus group (OP model rats injected with BMP2 overexpressing adenovirus into the femoral cavity) using a random number table method, with 9 rats in each group. Rats were euthanized after 2 months of injection, and femoral tissue was taken for micro computed tomography (micro-CT) three-dimensional reconstruction to observe the microstructural characteristics of bone trabeculae, and calculate bone mineral density (BMD), trabecular number (Tb.N), trabecular thickness (Tb.Th), bone volume/total volume (BV/TV), and trabecular separation (Tb.Sp) values. Hematoxylin eosin staining, Masson staining, and tartrate-resistant acid phosphatase (TRAP) staining were used to observe the pathological damage, collagen content, and osteoclast count of femoral tissues in four group rats. Malondialdehyde (MDA), glutathione (GSH), superoxide dismutase (SOD), ferritin, hepcidin levels in serum were detected. Real-time quantitative PCR was used to detect BMP2 mRNA expression in femoral tissue. Western blotting were used to detect BMP2, ALP, OCN, OPN, RANKL, TRAP, CTX-I, hepcidin, FPN1, TfR1, DMT1 protein expression in femoral tissue.Results:The differences of BMD, Tb.N, Tb.Th, BV/TV, and Tb.Sp in sham group, model group, adenovirus group, and BMP2 overexpressing adenovirus group were statistically significant ( P<0.05), among them, BMD, Tb.N, Tb.Th, and BV/TV in model group were lower than those in sham group, Tb.Sp was higher than that in sham group, BMD, Tb.N, Tb.Th, and BV/TV in BMP2 overexpressing adenovirus group were higher than those in model group and adenovirus group, Tb.Sp was lower than that in model group and adenovirus group ( P<0.05). Sham group had intact bone trabeculae, abundant collagen fibers, and fewer osteoclasts in femoral tissues; Model group and adenovirus group had sparse bone trabeculae, severe loss of collagen fibers, and more osteoclasts; Bone trabeculae and collagen fibers in overexpression BMP2 adenovirus group were greater than those in model group and adenovirus group, osteoclasts were fewer than those in model group and adenovirus group. The differences of MDA, GSH, SOD, ferritin, and hepcidin in serum in four groups were statistically significant ( P<0.05), among them, MDA and ferritin in model group were higher than those in sham group, GSH, SOD, hepcidin were lower those in sham group, MDA and ferritin levels in overexpression BMP2 adenovirus group were lower those in model group and adenovirus group, GSH, SOD, hepcidin were higher than those in model group and adenovirus group ( P<0.05). BMP2 mRNA expression of femoral tissues in four groups were 1.00±0.10, 0.39±0.12, 0.39±0.08, and 3.46±0.41, respectively, BMP2 protein expression were 0.61±0.06, 0.17±0.06, 0.20±0.05, and 1.03±0.07, respectively, the differences were statistically significant ( P<0.05), among them, model group were lower those in sham group, overexpression BMP2 adenovirus group were higher than those in model group and adenovirus group ( P<0.05). The differences of ALP, OCN, OPN, RANKL, TRAP, CTX-I, hepcidin, FPN1, TfR1, and DMT1 expression of femoral tissues in sham group, model group, adenovirus group, and BMP2 overexpressing adenovirus group were statistically significant ( P<0.05), among them, ALP, OCN, OPN, and hepcidin in model group were lower than those in sham group, RANKL, TRAP, CTX-I, FPN1, TfR1, and DMT1 were higher than those in sham group, ALP, OCN, OPN, and hepcidin in BMP2 overexpressing adenovirus group were higher than those in model group and adenovirus group, RANKL, TRAP, CTX-I, FPN1, TfR1, and DMT1 were lower than those in model group and adenovirus group ( P<0.05). Conclusion:BMP2 intervention iron metabolism related protein expression promotes bone formation in OP rats by increasing hepcidin level.

Objective:To evaluate the effectiveness and safety of allogeneic hematopoietic stem cell transplantation (allo-HSCT) in children with stage 4/M neuroblastoma (NB).Methods:This study was a prospective, single-arm, multicenter clinical trial conducted by Sun Yat-sen Memorial Hospital, Children′s Hospital of Nanjing Medical University, Children′s Hospital, Zhejiang University School of Medicine, the First Affiliated Hospital of Guangxi Medical University, Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine. From March, 2019 to August, 2023, 25 children with confirmed with stage 4/M NB and received allo-HSCT were enrolled. The patients received either unrelated cord blood transplantation (UCBT) or peripheral blood stem cell transplantation (PBSCT). Conditioning regimens for UCBT was fludarabine+busulfan+cyclophosphamide+topotecan, and for PBSCT was fludarabine+busulfan+melphalan+thiotepa+antithymocyte globulin, respectively. Until the last follow-up date of September, 2023, the overall survival (OS) rate and event free survival (EFS) rate were analyzed to evaluate efficacy. The engraftment rate and transplant-related complications were statistically assessed to evaluate safety. Survival analysis was performed using the Kaplan-Meier method.Results:Of the 25 patients, there were 15 males and 10 females. The age at transplantation was 5.7 (3.8, 7.3) years. The engraft rate was 100%, with recovery time of neutrophil as 15.7 (12.5, 17.0) d, and the recovery time of platelets as 33.5 (18.0, 48.0) d. Seventeen of the 25 children (68%) developed acute graft versus host disease (aGVHD), occurred at 18.0 (13.0, 22.5) d after transplantation, including 13 of grade Ⅲ-Ⅳ cases. The main sites of aGVHD were skin and intestinal tract. After treatment, 13 cases improved, 4 patients developed chronic graft-versus-host disease (cGVHD). After allo-HSCT, 14 children received maintenance therapy. Twenty of the 25 patients survived, the 2-year cumulative OS rate was (80±9)%, and 2-year EFS rate was (56±11)%. Nine cases (36%) relapsed, the time from allo-HSCT to disease relapse was 10.9 (5.5, 16.0) months. Five cases (20%) died. The hematopoietic stem cell transplantation associated mortality rate was 4% (1/25).The 2-year OS rate of patients who had partial remission prior to allo-HSCT was significant lower than those who had complete remission prior to allo-HSCT ((33±25)% vs. 100%, P=0.037). Conclusion:allo-HSCT is an effective treatment for patients with stage 4/M NB.