ObjectiveTo investigate the features of liver histopathological damage in patients with indeterminate phase of chronic HBV infection， as well as the timing for initiating antiviral therapy in such patients. MethodsA retrospective screening was performed for the patients with chronic HBV infection who were hospitalized in The Fifth Medical Center of Chinese PLA General Hospital and underwent liver biopsy from March 2018 to April 2022， among whom the patients who met the criteria for indeterminate phase defined in Chinese guidelines for chronic hepatitis B prevention and treatment （2022 edition） were enrolled， and their clinical data were collected. Liver histopathological stage was determined using the Scheuer scoring system， with stages 0 — 4 for inflammation grade （G） and stages 0 — 4 for fibrosis degree （S）， and the patients were divided into groups based on the presence of significant necroinflammation （≥G2） and significant liver fibrosis （≥S2）. The independent samples t-test was used for comparison of normally distributed continuous data between two groups， and the Mann-Whitney U test was used for comparison of non-normally distributed continuous data between two groups； the chi-square test or the Fisher’s exact test was used for comparison of categorical data between two groups. A Spearman’s rank correlation analysis was used to investigate the correlation between liver histopathology and clinical factors， and the Logistic regression model was used to identify the independent influencing factors for significant necroinflammation and liver fibrosis. ResultsA total of 271 patients with indeterminate phase of chronic HBV infection were enrolled， among whom 61 （22.5%） had significant necroinflammation （≥G2） and 124 （45.8%） had significant liver fibrosis （≥S2）. The Logistic regression analysis showed that alanine aminotransferase ≥30 U/L （for male patients） or ≥19 U/L （for female patients） （odds ratio ［OR］=2.69， 95% confidence interval ［CI］： 1.39 — 5.21， P=0.003）， HBV DNA ≥2 000 IU/mL （OR=2.75， 95%CI： 1.38 — 5.48， P=0.004）， and liver stiffness measurement （LSM） ≥6.0 kPa （OR=4.57， 95%CI： 2.17 — 9.62， P<0.001） were independent risk factors for significant inflammation. HBV DNA ≥2 000 IU/mL （OR=1.82， 95%CI： 1.01 — 3.32， P=0.049） and LSM ≥6.0 kPa （OR=2.06， 95%CI： 1.23 — 3.43， P=0.006） were independent influencing factors for significant liver fibrosis. ConclusionAmong the patients with indeterminate phase of chronic HBV infection， a substantial proportion of patients have significant liver histopathological damage. Antiviral therapy should be initiated in a timely manner for patients with high-risk factors.

ObjectiveTo understand the epidemiological and etiological characteristics of food-borne diseases in Wenchang， Hainan Province， and to analyze the suspected food exposure sites and sources so as to provide scientific data for early prevention. MethodsThe data of food-borne disease cases， including basic information， time， population， region， suspected exposed food， etiology， etc.， from the sentinel hospitals of the surveillance systems in Wenchang from 2017 to 2021 were statistically analyzed. ResultsA total of 2 219 food-borne disease cases were collected from 2017 to 2021. The male-to-female sex ratio was 1.2：1， and there was no significant statistical difference （χ²=5.732， P>0.05）. Cases were mainly concentrated in children aged <7 years （43.26%）， followed by 7 to 18 years （15.41%）， 19 to 30 years （11.72%） and >60 years （11.36%）， and there was a significant statistical difference （χ²=168.125， P<0.001）. The highest number of cases were found in preschool children （36.05%）， followed by students （17.49%） and farmers （16.18%）. The peak incidence occurred from July to November （54.44%）. The suspected exposure foods reported in the monitored cases were mainly vegetable foods （17.49%） and mixed foods （17.08%）. The exposure sites were primarily households （76.30%） and catering services （13.56%）. The positive rate of pathogens was 12.26% in 1085 tested samples， and there was no statistically significant difference in the annual detection rate （χ²=1.086， P>0.05）. The main pathogens of food-borne pathogens were Salmonella （9.95%）， followed by norovirus （2.03%） and rotavirus （0.28%）. ConclusionThe surveillance of foodborne disease cases in Wenchang shows seasonal peak patterns according to population and pathogen epidemiological features. The main affected groups are preschool children， students， farmers， and the elderly. The exposures are mainly related to vegetable foods and mixed foods in households and catering service establishments. Efforts should be made to strengthen food safety awareness， health education， and supervision targeting residents and food operators in order to reduce the incidence of foodborne diseases in the Wenchang area.

HBeAg seroconversion refers to the disappearance of HBeAg and the appearance of anti-HBe in chronic hepatitis B (CHB) patients with positive HBeAg in the past. HBeAg seroconversion marks the reductions in viral replication, immune tolerance, and liver inflammation and is an important monitoring indicator for evaluating disease conditions and the effect of CHB antiviral therapy, and it also indicates the endpoint of satisfactory treatment. Exploring the influencing factors for HBeAg seroconversion is of great significance to the selection of treatment regimens and the prognostic evaluation of CHB patients. This article mainly elaborates on the association of HBV with HBeAg seroconversion in CHB patients from the aspects of virological factors, host genetic factors, drug factors, and immunological factors.

Objective:To explore the teaching effect of magnetic on-demand teaching mode in nursing interns of neurology department.Methods:Forty-two nursing students who practiced in Neurology Department of Haikou People's Hospital from July 2019 to December 2019 were selected as the control group, and 44 nursing students who practiced from January 2020 to June 2020 were selected as the study group. The traditional teaching mode was adopted in the control group, while the magnetic on-demand teaching mode was adopted in the study group. The scores of theory and skill examination after teaching, nurses' core competence (evaluated by nurse core competence scale) before and after teaching, active learning rate and incidence of nursing errors during teaching and satisfaction with teaching were compared between the two groups. SPSS 25.0 was used for t test and chi-square test. Results:After teaching, the scores of theory and skills in the study group were significantly higher than those in the control group ( P<0.05). After teaching, the scores of core competence of nurses in the two groups were higher than before, and the scores of core competence of nurses in the study group were higher ( P<0.05). During the teaching period, the active learning rate of the study group was higher than that of the control group ( P<0.05), and the incidence rate of nursing errors was lower than that of the control group ( P<0.05). The satisfaction of all kinds of teaching in the study group was higher than that in the control group, with statistically significant differences. Conclusion:In the clinical teaching of nursing interns in neurology department, the magnetic on-demand teaching mode can significantly enhance the mastery of nursing theory and skills, improve the core competence of nurses, improve the active learning rate and teaching satisfaction, and reduce the occurrence of nursing errors.

Objective:To investigate the expression and clinical significance of metastasis suppressor gene Kiss-1 and KAI-1 in colorectal cancer. Methods: In situ hybridization was used to detect and analyze the gene expression of Kiss-1 and KAI-1 in 61 cases of colorectal cancer and 20 cases of normal para cancer tissue. Results: In situ hybridization revealed that the positive expression rates of Kiss-1 and KAI-1 in colorectal cancer were significantly lower than those in normal para cancer tissue (P

AIM: To measure microcirculation function in type 2 diabetic patients and non-diabetic subjects with a new measurement method called capillary recruitment. METHODS: 276 type 2 diabetic patients in Shanghai downtown were enrolled and categorized into several groups, those with diabetes duration