Objective To establish a supported liquid-liquid extraction-ultra-performance liquid chromatography-tandem mass spectrometry(SLE-UPLC-MS/MS)method for detecting methoxetamine(MXE,2-(3-methoxyphenyl)-2-(ethylamino)cyclohexanone)and 2-oxo-PCE(2-(ethylamino)-2-phenylcyclohexan-1-one)in blood.Methods Blood samples were extracted and purified using supported liquid-liquid extraction columns.An electrospray ionization source and multiple reaction monitoring mass spectrometry were used for qualitative and quantitative analysis of MXE and 2-oxo-PCE in blood samples.Results The limits of detection for MXE and 2-oxo-PCE in blood were 0.15 ng/mL and 0.17 ng/mL,respectively.Both compounds showed good linearity in the range of 0.5-500 ng/mL(r2>0.999),with recovery rates ranging from 90.7%to 98.4%.Conclusion The established qualitative and quantitative analytical method is simple,rapid,reliable,and highly sensitive,making it suitable for the detection and identification of MXE and 2-oxo-PCE in blood.

Nonunion of osteoporotic vertebral fractures (OVF), predominantly affecting the elderly, can lead to intractable pain, vertebral collapse, progressive kyphotic deformity, and neurological impairment, significantly compromising patients′ quality of life. There exists considerable debate on diagnosis and management of OVF, encompassing key issues such as clinical diagnosis and staging criteria for nonunion, surgical indications and procedure selection, and postoperative rehabilitation planning. Currently, there lacks standardized clinical guideline and expert consensus on the diagnosis and management of OVF nonunion in China. To address this gap, Minimally Invasive Surgery Group of Chinese Orthopedic Association, Osteoporosis Committee of Chinese Association of Orthopedic Surgeons, Prevention and Rehabilitation Committee for Osteoporosis of Chinese Association of Rehabilitation Medicine and Minimally Invasive Orthopedic Surgery Branch of China Association for Geriatric Care jointly organized domestic experts in spinal surgery, endocrinology, and rehabilitation to formulate the Clinical guideline for the diagnosis and treatment for nonunion of osteoporotic vertebral fractures ( version 2025), based on existing literature and clinical experience and adhering to principles of scientific rigor and practicality. The guideline provided 13 evidence-based recommendations encompassing diagnosis and treatment of OVF nonunion, aiming to standardize its clinical management.

Objective To establish a supported liquid-liquid extraction-ultra-performance liquid chromatography-tandem mass spectrometry(SLE-UPLC-MS/MS)method for detecting methoxetamine(MXE,2-(3-methoxyphenyl)-2-(ethylamino)cyclohexanone)and 2-oxo-PCE(2-(ethylamino)-2-phenylcyclohexan-1-one)in blood.Methods Blood samples were extracted and purified using supported liquid-liquid extraction columns.An electrospray ionization source and multiple reaction monitoring mass spectrometry were used for qualitative and quantitative analysis of MXE and 2-oxo-PCE in blood samples.Results The limits of detection for MXE and 2-oxo-PCE in blood were 0.15 ng/mL and 0.17 ng/mL,respectively.Both compounds showed good linearity in the range of 0.5-500 ng/mL(r2>0.999),with recovery rates ranging from 90.7%to 98.4%.Conclusion The established qualitative and quantitative analytical method is simple,rapid,reliable,and highly sensitive,making it suitable for the detection and identification of MXE and 2-oxo-PCE in blood.

Nonunion of osteoporotic vertebral fractures (OVF), predominantly affecting the elderly, can lead to intractable pain, vertebral collapse, progressive kyphotic deformity, and neurological impairment, significantly compromising patients′ quality of life. There exists considerable debate on diagnosis and management of OVF, encompassing key issues such as clinical diagnosis and staging criteria for nonunion, surgical indications and procedure selection, and postoperative rehabilitation planning. Currently, there lacks standardized clinical guideline and expert consensus on the diagnosis and management of OVF nonunion in China. To address this gap, Minimally Invasive Surgery Group of Chinese Orthopedic Association, Osteoporosis Committee of Chinese Association of Orthopedic Surgeons, Prevention and Rehabilitation Committee for Osteoporosis of Chinese Association of Rehabilitation Medicine and Minimally Invasive Orthopedic Surgery Branch of China Association for Geriatric Care jointly organized domestic experts in spinal surgery, endocrinology, and rehabilitation to formulate the Clinical guideline for the diagnosis and treatment for nonunion of osteoporotic vertebral fractures ( version 2025), based on existing literature and clinical experience and adhering to principles of scientific rigor and practicality. The guideline provided 13 evidence-based recommendations encompassing diagnosis and treatment of OVF nonunion, aiming to standardize its clinical management.