Objective:To study the relationship between intraoperative portal venous pressure gradient (PVPG) changes and clinical outcomes of meso-Rex bypass in pediatric patients with extrahepatic portal vein obstruction (EHPVO).Methods:Clinical data of 76 children with EHPVO undergoing Rex shunt at Guangzhou Women and Children’s Medical Center, Affiliated to Guangzhou Medical University from April 2018 to October 2021 were retrospectively analyzed, including 49 males and 27 females, aged 62.0 (42.0, 102.5) months. Logistic regression was used to identify prognostic factors. Receiver operating characteristic (ROC) curves were used to evaluate the diagnostic efficacy of PVPG variations. The association between pre-/postoperative PVPG differences and clinical outcomes (resolution of hypersplenism/splenomegaly, improvement of gastroesophageal varices, and vascular complications) were systematically assessed.Results:All 76 pediatric patients underwent the Rex shunt successfully. Postoperative vascular complications occurred in 14 cases (18.4%), including six cases of bypass graft thrombosis (7.9%) and eight cases of anastomotic stenosis (10.5%). Logistic regression analyses revealed that graft type ( OR=0.03, 95% CI: 0-0.30, P=0.003) and PVPG gradient ( OR=1.65, 95% CI: 1.07-2.53, P=0.022) were associated with esophagogastric varices resolution after Rex shunt. Graft type also correlated with splenomegaly/hypersplenism improvement ( OR=0.08, 95% CI: 0.01-0.80, P=0.032). PVPG gradient showed association with anastomotic stenosis ( OR=0.69, 95% CI: 0.51-0.93, P=0.014). The area under ROC curves of PVPG gradient are 0.786 and 0.815 for predicting varices resolution and anastomotic stenosis, respectively. Conclusion:An increased intraoperative PVPG gradient could serve as a protective factor for an improved outcome of Rex shunt in EHPVO children and reduced risk of anastomotic stenosis.

Objective:To study the relationship between intraoperative portal venous pressure gradient (PVPG) changes and clinical outcomes of meso-Rex bypass in pediatric patients with extrahepatic portal vein obstruction (EHPVO).Methods:Clinical data of 76 children with EHPVO undergoing Rex shunt at Guangzhou Women and Children’s Medical Center, Affiliated to Guangzhou Medical University from April 2018 to October 2021 were retrospectively analyzed, including 49 males and 27 females, aged 62.0 (42.0, 102.5) months. Logistic regression was used to identify prognostic factors. Receiver operating characteristic (ROC) curves were used to evaluate the diagnostic efficacy of PVPG variations. The association between pre-/postoperative PVPG differences and clinical outcomes (resolution of hypersplenism/splenomegaly, improvement of gastroesophageal varices, and vascular complications) were systematically assessed.Results:All 76 pediatric patients underwent the Rex shunt successfully. Postoperative vascular complications occurred in 14 cases (18.4%), including six cases of bypass graft thrombosis (7.9%) and eight cases of anastomotic stenosis (10.5%). Logistic regression analyses revealed that graft type ( OR=0.03, 95% CI: 0-0.30, P=0.003) and PVPG gradient ( OR=1.65, 95% CI: 1.07-2.53, P=0.022) were associated with esophagogastric varices resolution after Rex shunt. Graft type also correlated with splenomegaly/hypersplenism improvement ( OR=0.08, 95% CI: 0.01-0.80, P=0.032). PVPG gradient showed association with anastomotic stenosis ( OR=0.69, 95% CI: 0.51-0.93, P=0.014). The area under ROC curves of PVPG gradient are 0.786 and 0.815 for predicting varices resolution and anastomotic stenosis, respectively. Conclusion:An increased intraoperative PVPG gradient could serve as a protective factor for an improved outcome of Rex shunt in EHPVO children and reduced risk of anastomotic stenosis.

ObjectiveTo analyze the induction effect of Fusobacterium nucleatum （Fn） on endoplasmic reticulum stress-related proteins Glucose-regulating protein 78（GRP78） and X-box binding protein 1（XBP1） in esophageal squamous cell carcinoma （ESCC）， and to explore its potential mechanism and clinical significance. MethodsESCC cells KYSE150 and KYSE140 were infected with Fn for 12 h， 24 h and 48 h. The oxidative stress indexes （ROS， MDA and SOD） and the expression of GRP78 and XBP1 in each group were detected by oxidative stress index kit and Western blot. The experiment was divided into Fn groups， Fn+siNC1 groups， Fn+siGRP78 groups， Fn+siNC2 groups and Fn+siXBP1 groups； the oxidative stress indexes， paclitaxel （PTX） response efficacy， abilities of proliferation， invasion and metastasis in each group were compared. The infection of Fn and the expression of GRP78 and XBP1 in 234 ESCC and paracancerous tissues were detected by RNA scope and immunohistochemistry. The correlation between each factor and clinicopathological characteristics of patients was analyzed by Chi-square test. The influence of each factor on the survival of patients was compared by Kaplan-meier survival estimate. ResultsCompared with Fn uninfected KYSE150 and KYSE140 cells， the content of ROS and MDA was gradually increased， the activity of SOD was gradually decreased， and the expression of GRP78 and XBP1 was gradually increased in Fn infected groups （12 h， 24 h and 48 h） （P < 0.05）. Compared with Fn groups， Fn+siNC1 groups， and Fn+siNC2 groups， ROS and MDA contents were decreased， SOD activity was increased， PTX response efficacy was enhanced， and abilities of proliferation， invasion and metastasis were decreased in Fn+siGRP78 and Fn+siXBP1 groups （P < 0.05）. The rates of Fn， GRP78 and XBP1 in ESCC tissues were 43.16%， 69.66% and 60.68%， respectively. And the three indexes were significantly consistent （P < 0.05）. The patients with positive Fn infection and high expression of GRP78 and XBP1 were mostly males with a history of smoking and drinking， and the tumor differentiation degree was low， the invasion degree was deep， the lymph node metastasis rate was high， and the clinical stage was mostly stage Ⅲ/Ⅳ. The 5-year survival time of patients with above positive indexes was shortened （P < 0.05）. ConclusionsFn could induce endoplasmic reticulum stress by inducing the high expression of GRP78 and XBP1， and promote the malignant evolution of ESCC.

Clear cell renal cell carcinoma (ccRCC) has been proved to be a metabolic disease with high

Humans ; Joint Dislocations ; Lunate Bone ; Wrist Injuries

OBJECTIVE: To evaluate the outcomes and to find out the influence factors of indirect pulp treatment in primary teeth. METHODS: Children who received indirect pulp treatment in primary teeth in the Department of Pediatric Dentistry in Peking University School and Hospital of Stomatology from August, 2014 to September, 2016 were collected in the Electronic Medical Record Database of Peking University School and Hospital of Stomatology, via the database for scientific research provided by the corporation of Kaientai. The children selected as the subjects of this study were followed up over 1.5 years, and they were under 9 years old if in the group of primary molars while the children in the group of primary anterior teeth were under 4 and a half years old. Those children who were not reviewed regularly or didn't have complete medical records were removed. Basic information, the relevant medical records and radiographic records of those children were collected. All teeth were examined clinically and classified into 2 outcomes, teeth in group H were regarded as succeeded, and teeth in group P were regarded as failed. Survival analysis was applied. The survival rate and survival time of the deciduous teeth calculated. Multivariate analysis was performed by using Cox proportional hazard model. RESULTS: One hundred and six children were finally included, aged from 1.6 to 8.8 years, with the mean age of (5.0±1.7) years. 168 primary teeth (122 primary molars, 46 primary anterior teeth) were included, and the average follow up time was (729±244) days. Thirty-five primary teeth (23 primary molars, 12 primary anterior teeth) failed upon clinical or radiographic examinations by September, 2018. The cumulative survival probability of half a year, one year, one year and a half, two years, two and a half years for the indirect pulp treatment was 93.5%, 92.9%, 87.5%, 82.7%, and 75.5% through the KaplanMeier method, respectively. Through the analysis of Cox proportional hazard model, in primary molars, the survival probability tended to be lower when the number of tooth surface affected by caries was greater (OR=1.709, P<0.05). Compared with primary molars, the survival probability of primary anterior teeth was lower, but the difference was not significant. CONCLUSION Complying the current instructions in our department, the cumulative survival probability of two and a half years after the indirect pulp treatment in primary teeth was 75.5%. In primary molars, the survival probability tended to be lower when the number of tooth surfaces affected by caries increased.
Child ; Child, Preschool ; Dental Caries ; Dental Pulp ; Humans ; Infant ; Molar ; Retrospective Studies ; Tooth, Deciduous

This review summarised and compared the contents and methods of America,s Health Rankings (AHR)and the County Health Rankings(CHR)in the United States by using literature review.AHR and CHR are the currently widely used population health assessment index ranking systems in the United States,respectively,which provided an analysis of population health on a state-by-state basis and a county-by-county basis by evaluating a histori-cal and comprehensive set of health outcomes and health determinants data to determine the health benchmarks and state/county rankings.The selection criteria of each indicator take into account of reliability, availability, stability, and intervention.The determination of weights of each indicator took into considerations the literature review,a histori-cal perspective,weights used by other rankings,internal analyses of the variation in outcomes explained by each factor, and pragmatic issues involving communications and stakeholder engagement.By comparison, it was found that AHR and CHR have a higher level of accuracy in the classification as they are well -defined by population demographics and geography respectively.The evaluation of health cities and health villages and towns in China is more complicated. There are few studies on village and town health rankings systems.This article reviewed the evaluation methods of AHR and CHR with a view to providing a reference for research on the evaluation system of City Health Rankings and the Village and Town Health Rankings in China.

This study was aimed to qualitatively analyze the chemical components in Congrong Zonggan capsule by using ultra-performance liquid chromatography tandem quadrupole time-of-flight mass spectrometry method (UPLC-Q-TOF-MS/MS). An Agilent SB-C₁₈ Rapid Resolution HD (3.0 mm×100 mm,1.8 μm) was used with acetonitrile (A) - 0.1% formic acid solution (B) as the mobile phase for gradient elution. The flow rate was 0.2 mL•min⁻¹; the detection wavelength was set at 330 nm and the column temperature was maintained at 30 ℃. Electrospray ion (ESI) source was applied for the qualitative analysis under the negative ion mode. Finally, based on comparison with standard samples, database matching analysis and reviewing relevant literature, 41 compounds were identified from Congrong Zonggan capsule. This method could be used to rapidly detect the chemical components in Congrong Zonggan capsule, providing reference for the quality control of Congrong Zonggan capsule and laying a foundation for the further study on active components mechanism.

OBJECTIVETo study a family with Bw subtype of ABO blood group system, and to review safety issues in relation with clinical transfusion.

METHODSThe molecular basis for the blood type was studied with serological assay, polymerase chain reaction-sequence specific primer (PCR-SSP) and DNA sequencing, TA clone and haplotype analysis in one blood donor whose ABO blood group were difficulty typed and her family. The bioinformatics analysis was carried out by biological analysis software to investigate the change of structure and function of enzymes influenced by the change amino acid. A retrospective survey was carried out to investigate what is the actual position that the donor blood was used in the clinical transfusion.

RESULTSThree members from the family were found to have a Bw subtype. A substitution of nucleotide C by T at position 721 in exon 7 was discovered, which resulted in replacement of amino acid Arg to Trp. Review of clinical record suggested that there has been no significant abnormality association with past three blood transfusions.

CONCLUSIONA 721C>T mutation of the ABO gene probably underlies the Bw subtype. Further research is needed for understanding the clinical significance of this subtype in the blood transfusion.

ABO Blood-Group System ; classification ; genetics ; Adult ; Amino Acid Sequence ; Base Sequence ; Blood Transfusion ; Exons ; Female ; Humans ; Male ; Molecular Sequence Data ; Pedigree ; Polymerase Chain Reaction ; Retrospective Studies