This study investigates the expression pattern and functional significance of EF-hand domain-containing protein 2 (EFHD2) in hepatocellular carcinoma (HCC), with particular focus on its regulatory effects on tumor proliferation, migration, and invasion. Cellular experimental study was completed from June 2024 to January 2025 in the Basic Laboratory of the General Hospital of Southern Theater Command. TCGA database to determine EFHD2 expression and its clinicopathological correlations. GSCA database to assess methylation patterns and immune infiltration. Model of transient overexpression and knockdown of EFHD2 was constructed in hepatocellular carcinoma cells Hep3B, then RT-qPCR and Western blot were applied to verify the transfection efficiency. CCK-8 and colony formation assays for proliferation assessment, Transwell chambers for migration/invasion quantification. Protein-protein interaction networks were constructed via STRING, followed by GO/KEGG enrichment analysis. Statistical analysis was performed using the two independent samples t-test. The results showed that EFHD2 demonstrated significant upregulation in HCC tissues versus normal controls ( P<0.05). Elevated EFHD2 expression correlated with advanced clinical stage ( P<0.05) and poor differentiation ( P<0.05). In the CCK-8 assay, the EFHD2 overexpression group demonstrated significantly higher cell viability than the control group, as evidenced by 450 nm relative absorbance values on Day 1 (0.529±0.019 vs. 0.515±0.016, F=0.041, P=0.320), Day 2 (1.356±0.019 vs. 1.094±0.042, F=3.833, P<0.001), Day 3 (2.817±0.049 vs. 2.143±0.124, F=3.833, P<0.001), and Day 4 (3.848±0.015 vs. 3.430±0.021, F=0.469, P<0.001). The EFHD2 knockdown group showed reduced cell viability compared to controls: Day 1 (0.541±0.020 vs. 0.552±0.015, F=0.098, P=0.423), Day 2 (1.154±0.009 vs. 1.326±0.029, F=2.485, P<0.001), Day 3 (2.453±0.041 vs. 2.653±0.031, F=0.479, P<0.001), and Day 4 (3.685±0.038 vs. 3.836±0.021, F=6.804, P<0.001). In colony formation assays, the overexpression group displayed a significant increase in colony numbers (254.667±23.861 vs. 186.000±16.703, F=0.865, P=0.015), whereas the knockdown group exhibited decreased colony formation (229.000±24.637 vs. 306.667±36.501, F=0.988, P=0.038). In Transwell assays, the EFHD2 overexpression group revealed enhanced migratory capacity [ (605.000±72.670) cells vs. (472.667±28.095) cells, F=2.462, P=0.042] and invasive potential [(767.333±21.221) cells vs. (414.333±16.623) cells, F=0.331, P<0.001]. The knockdown group showed attenuated migration [(311.000±71.084) cells vs. (479.667±50.846) cells, F=0.718, P=0.029] and invasion [(247.667±48.263) cells vs. (345.667±32.130) cells, F=0.727, P=0.043] compared to controls. The network of EFHD2-interacting proteins was further constructed by the STRING database, and the GO and KEGG analysis were used to perform bioinformatics analysis reveal that EFHD2 is mainly involved in actin cytoskeleton regulation. In conclusion, EFHD2 is highly expressed in HCC and is involved in the process of proliferation, migration and invasion of HCC.

Changes in hair follicle stem cells (HFSCs) can affect scalp aging and hair growth. With increasing age, HFSCs exhibit a decrease in quiescence maintenance and self-renewal capacity, as well as differentiation potential, leading to shortened hair growth cycles and even hair loss. This review summarizes recent research advances in the multifactorial interactions underlying hair loss, including the regulatory mechanisms of HFSC quiescence, the impact of aging on HFSC function, and aging of the stem cell microenvironment. Additionally, this review discusses the relationship between stem cells and hair shafts, and the mechanisms of action of stem cells in scalp aging, including alterations in signaling pathways, chromatin remodeling, and epigenetic regulation, etc. Furthermore, stem cell-based therapeutic strategies are summarized, such as the use of stem cells or their secreting exosomes, modulation of the stem cell microenvironment, and pharmacological interventions.

OBJECTIVE To observe the distribution and drug resistance of pathogens isolated from the neonates with purulent meningitis and analyze the risk factors for prognosis.METHODS A total of 80 neonates with puru-lent meningitis who were treated in Maternity and Child Care Center of Xinyu and Xinyu People's Hospital from Jan.2020 to Dec.2022 were recruited as the research subjects.The distribution and drug resistance of the patho-gens isolated from the patients were observed.The neonates were divided into the poor prognosis group with 31 cases and the favorable prognosis group with 49 cases according to the treatment outcomes at the discharge from the hospital.The clinical data were compared between the two groups.The risk factors for the prognosis were ana-lyzed.RESULTS Totally 84 strains of pathogens were isolated from 80 children,the gram-positive bacteria accoun-ted for 36.90％,mainly including Staphylococcus epidermidis,Staphylococcus haemolyticus,Staphylococcus saprophyticus and Staphylococcus hominis;The gram-negative bacteria accounted for 63.10％,mainly including Escherichia coli and Klebsiella pneumoniae.The drug resistance rates of the major species of gram-positive bacte-ria to oxacillin,penicillin,cefoxitin and clindamycin were high.The drug resistance rates of the major species of gram-negative bacteria to levofloxacin,gentamycin and ceftriaxone were high.The drug resistance rates of the K.pneumoniae strains to gentamycin,levofloxacin and tobramycin were high.The proportion of neonates with coma and the total protein level of cerebrospinal fluid were higher in the poor prognosis group than in the favorable prog-nosis group(P＜0.05);the cerebrospinal fluid glucose level of the poor prognosis group was lower than that of the favorable prognosis group(P＜0.05).Coma,high level of total protein in cerebrospinal fluid and low level of cere-brospinal fluid glucose were the risk factors for the poor prognosis(P＜0.05).CONCLUSIONS The gram-negative bacteria are dominant among the pathogens isolated from the neonates with purulent meningitis.The coma,total protein of cerebrospinal fluid and cerebrospinal fluid glucose are the risk factors for the poor prognosis.It is neces-sary for the hospital to rational use of antibiotics based on the results of culture of pathogens and drug susceptibili-ty testing and take intervention measures to the risk factors for the prognosis.

This study investigates the expression pattern and functional significance of EF-hand domain-containing protein 2 (EFHD2) in hepatocellular carcinoma (HCC), with particular focus on its regulatory effects on tumor proliferation, migration, and invasion. Cellular experimental study was completed from June 2024 to January 2025 in the Basic Laboratory of the General Hospital of Southern Theater Command. TCGA database to determine EFHD2 expression and its clinicopathological correlations. GSCA database to assess methylation patterns and immune infiltration. Model of transient overexpression and knockdown of EFHD2 was constructed in hepatocellular carcinoma cells Hep3B, then RT-qPCR and Western blot were applied to verify the transfection efficiency. CCK-8 and colony formation assays for proliferation assessment, Transwell chambers for migration/invasion quantification. Protein-protein interaction networks were constructed via STRING, followed by GO/KEGG enrichment analysis. Statistical analysis was performed using the two independent samples t-test. The results showed that EFHD2 demonstrated significant upregulation in HCC tissues versus normal controls ( P<0.05). Elevated EFHD2 expression correlated with advanced clinical stage ( P<0.05) and poor differentiation ( P<0.05). In the CCK-8 assay, the EFHD2 overexpression group demonstrated significantly higher cell viability than the control group, as evidenced by 450 nm relative absorbance values on Day 1 (0.529±0.019 vs. 0.515±0.016, F=0.041, P=0.320), Day 2 (1.356±0.019 vs. 1.094±0.042, F=3.833, P<0.001), Day 3 (2.817±0.049 vs. 2.143±0.124, F=3.833, P<0.001), and Day 4 (3.848±0.015 vs. 3.430±0.021, F=0.469, P<0.001). The EFHD2 knockdown group showed reduced cell viability compared to controls: Day 1 (0.541±0.020 vs. 0.552±0.015, F=0.098, P=0.423), Day 2 (1.154±0.009 vs. 1.326±0.029, F=2.485, P<0.001), Day 3 (2.453±0.041 vs. 2.653±0.031, F=0.479, P<0.001), and Day 4 (3.685±0.038 vs. 3.836±0.021, F=6.804, P<0.001). In colony formation assays, the overexpression group displayed a significant increase in colony numbers (254.667±23.861 vs. 186.000±16.703, F=0.865, P=0.015), whereas the knockdown group exhibited decreased colony formation (229.000±24.637 vs. 306.667±36.501, F=0.988, P=0.038). In Transwell assays, the EFHD2 overexpression group revealed enhanced migratory capacity [ (605.000±72.670) cells vs. (472.667±28.095) cells, F=2.462, P=0.042] and invasive potential [(767.333±21.221) cells vs. (414.333±16.623) cells, F=0.331, P<0.001]. The knockdown group showed attenuated migration [(311.000±71.084) cells vs. (479.667±50.846) cells, F=0.718, P=0.029] and invasion [(247.667±48.263) cells vs. (345.667±32.130) cells, F=0.727, P=0.043] compared to controls. The network of EFHD2-interacting proteins was further constructed by the STRING database, and the GO and KEGG analysis were used to perform bioinformatics analysis reveal that EFHD2 is mainly involved in actin cytoskeleton regulation. In conclusion, EFHD2 is highly expressed in HCC and is involved in the process of proliferation, migration and invasion of HCC.

Changes in hair follicle stem cells (HFSCs) can affect scalp aging and hair growth. With increasing age, HFSCs exhibit a decrease in quiescence maintenance and self-renewal capacity, as well as differentiation potential, leading to shortened hair growth cycles and even hair loss. This review summarizes recent research advances in the multifactorial interactions underlying hair loss, including the regulatory mechanisms of HFSC quiescence, the impact of aging on HFSC function, and aging of the stem cell microenvironment. Additionally, this review discusses the relationship between stem cells and hair shafts, and the mechanisms of action of stem cells in scalp aging, including alterations in signaling pathways, chromatin remodeling, and epigenetic regulation, etc. Furthermore, stem cell-based therapeutic strategies are summarized, such as the use of stem cells or their secreting exosomes, modulation of the stem cell microenvironment, and pharmacological interventions.

Objective:To explore the application effect of EBN-5A framework nursing intervention in elderly patients with recurrent ischemic stroke after revascularization.Methods:Using the convenience sampling method, 127 elderly patients with recurrent ischemic stroke after revascularization admitted to Zhumadian Central Hospital from April 2021 to April 2023 were selected as the research objects. According to the random number table method, the patients were divided into the experimental group ( n=64) and the control group ( n=63). The control group was given the traditional nursing mode of Neurology, and the experimental group was given the EBN-5A framework nursing intervention plan on the basis of the control group. The improvement rate of clinical symptoms, intracranial blood circulation, and Stroke Impact Scale (SIS) score were compared between the two groups. Results:The clinical improvement rate of the experimental group was higher than that of the control group, the cerebral blood flow and cerebral blood volume were higher than those of the control group, while the mean transit time and time to peak were lower than those of the control group, the SIS score was higher than that of the control group, and the differences were statistically significant ( P<0.05) . Conclusions:The EBN-5A framework nursing intervention plan can improve the clinical symptoms of elderly patients with recurrent ischemic stroke after revascularization, enhance the function of cranial blood vessels, improve the quality of life.

OBJECTIVE To observe the distribution and drug resistance of pathogens isolated from the neonates with purulent meningitis and analyze the risk factors for prognosis.METHODS A total of 80 neonates with puru-lent meningitis who were treated in Maternity and Child Care Center of Xinyu and Xinyu People's Hospital from Jan.2020 to Dec.2022 were recruited as the research subjects.The distribution and drug resistance of the patho-gens isolated from the patients were observed.The neonates were divided into the poor prognosis group with 31 cases and the favorable prognosis group with 49 cases according to the treatment outcomes at the discharge from the hospital.The clinical data were compared between the two groups.The risk factors for the prognosis were ana-lyzed.RESULTS Totally 84 strains of pathogens were isolated from 80 children,the gram-positive bacteria accoun-ted for 36.90％,mainly including Staphylococcus epidermidis,Staphylococcus haemolyticus,Staphylococcus saprophyticus and Staphylococcus hominis;The gram-negative bacteria accounted for 63.10％,mainly including Escherichia coli and Klebsiella pneumoniae.The drug resistance rates of the major species of gram-positive bacte-ria to oxacillin,penicillin,cefoxitin and clindamycin were high.The drug resistance rates of the major species of gram-negative bacteria to levofloxacin,gentamycin and ceftriaxone were high.The drug resistance rates of the K.pneumoniae strains to gentamycin,levofloxacin and tobramycin were high.The proportion of neonates with coma and the total protein level of cerebrospinal fluid were higher in the poor prognosis group than in the favorable prog-nosis group(P＜0.05);the cerebrospinal fluid glucose level of the poor prognosis group was lower than that of the favorable prognosis group(P＜0.05).Coma,high level of total protein in cerebrospinal fluid and low level of cere-brospinal fluid glucose were the risk factors for the poor prognosis(P＜0.05).CONCLUSIONS The gram-negative bacteria are dominant among the pathogens isolated from the neonates with purulent meningitis.The coma,total protein of cerebrospinal fluid and cerebrospinal fluid glucose are the risk factors for the poor prognosis.It is neces-sary for the hospital to rational use of antibiotics based on the results of culture of pathogens and drug susceptibili-ty testing and take intervention measures to the risk factors for the prognosis.

Objective:To explore the application effect of EBN-5A framework nursing intervention in elderly patients with recurrent ischemic stroke after revascularization.Methods:Using the convenience sampling method, 127 elderly patients with recurrent ischemic stroke after revascularization admitted to Zhumadian Central Hospital from April 2021 to April 2023 were selected as the research objects. According to the random number table method, the patients were divided into the experimental group ( n=64) and the control group ( n=63). The control group was given the traditional nursing mode of Neurology, and the experimental group was given the EBN-5A framework nursing intervention plan on the basis of the control group. The improvement rate of clinical symptoms, intracranial blood circulation, and Stroke Impact Scale (SIS) score were compared between the two groups. Results:The clinical improvement rate of the experimental group was higher than that of the control group, the cerebral blood flow and cerebral blood volume were higher than those of the control group, while the mean transit time and time to peak were lower than those of the control group, the SIS score was higher than that of the control group, and the differences were statistically significant ( P<0.05) . Conclusions:The EBN-5A framework nursing intervention plan can improve the clinical symptoms of elderly patients with recurrent ischemic stroke after revascularization, enhance the function of cranial blood vessels, improve the quality of life.

Objective To explore the expression level of serum long non-coding RNA(lncRNA)T342620 in patients with hepatocellular carcinoma(HCC)and the clinical value of single or combined detection with al-pha-fetoprotein(AFP)for HCC.Methods Case-control studies were conducted.A total of 69 patients with primary hepatocellular carcinoma(HCC group),32 patients with hepatitis B(hepatitis B group),20 patients with liver cirrhosis(liver cirrhosis group),30 patients after transcatheter hepatic arterial chemoembolization(TACE)for primary hepatocellular carcinoma(HCC postoperative group)and 50 healthy patients(health ex-amination group)treated in the General Hospital of Southern Theatre Command of PLA from April 2021 to May 2023 were selected as the study objects.The serum total RNA was extracted and the relative expression level of lncRNA T342620 in serum was detected by real-time quantitative PCR.Combined with the clinical di-agnosis and treatment data of patients,the correlation between its expression and pathological characteristics and serological indexes was analyzed,and the specificity and sensitivity of lncRNA T342620 alone and in com-bination with AFP in the diagnosis of HCC were analyzed by receiver operating characteristic(ROC)curve.The diagnostic efficacy was judged according to the area under the curve,and its application value in the diag-nosis of HCC was evaluated.The chi-square test was used for comparison between groups,and the Spearman method was used for correlation analysis.Results The serum expression levels of lncRNA T342620 in liver cancer group and postoperative liver cancer group were higher than those in healthy physical examination group,hepatitis B group and liver cirrhosis group,and the differences were statistically significant(P＜0.001).Clinical pathological and serological index analysis revealed that as the tumor size increased,the serum lncRNA-T342620 expression level also increased.In the HCC group,the serum lncRNA T342620 expression level was negatively correlated with albumin(ALB)and the A/G ratio(P＜0.05),while it was positively cor-related with α-L-fucosidase(AFU)and HBV-DNA(P＜0.05).In patients from the HCC postoperative group,the serum lncRNA T342620 expression level was positively correlated with total bile acid(TBA)(P＜0.05).ROC curve analysis demonstrated that when using serum lncRNA T342620 to distinguish,the sensitivi-ty and the specificity were 55.1％and 94.1％,respectively,indicating good diagnostic value.When combined with AFP detection,the sensitivity and the specificity improved to 91.3％and 91.2％,respectively,which were higher than those of individual indicators and had a superior diagnostic efficiency with area under the cuve(AUC)of 0.954 compared to AUC of AFP or lncRNA-T342620 alone(0.906,0.758),and the differ-ences were statistically significant(P＜0.05).Conclusion Serum lncRNA T342620 may be a new serological index for the auxiliary diagnosis of HCC.

Objective To analyze the correlation between cumulative fluid balance volume and increasing plasma volume and aggravated clinical congestion symptoms in patients with heart failure on admission for 1～7 d,and to explore the application value of cumulative fluid balance volume in predicting volume overload.Methods Using the convenience sampling method,235 heart failure patients hospitalized from October 2022 to February 2023 in a total of 3 tertiary hospitals in Nanjing,Lianyungang,Jiangsu Province,and Zhumadian,Henan Province,were selected and classified into an increasing/decreasing plasma volume group and an aggravated/alleviated clinical congestion symptoms group.General information,clinical characteristics,and 1～7 d cumulative fluid balance volume of the study subjects were collected to compare the differences in cumulative fluid balance volume between the 2 groups.Logistic regression was used to analyze the effect of cumulative fluid balance volume on plasma volume and clinical congestion symptoms.Receiver operating characteristic curves were used to analyze the optimal cutoff value of cumulative fluid balance volume for predicting increasing plasma volume and aggravated clinical congestion symptoms.Results Excluding 15 pat ients who were discharged early and 3 patients with inaccurate records of fluid intake and excretion,a total of 217 patients were included.The incidence of positive fluid balance was lowest on day 1,at 65.90%,with the smallest cumulative volume of(0.235±0.983)L;the highest incidence of positive balance occurred on day 6,at 75.58%,with the largest cumulative volume of(2.444±5.445)L.The cumulative fluid balance at 4～7 d in the increasing plasma volume group was higher than that in the decreasing plasma volume group,and the difference was statistically significant(P<0.05);the cumulative fluid balance at 4～7 d was an independent risk factor for plasma volume elevation,and a cumulative fluid balance of 2.308,3.361,3.518,and 3.702 L at 4～7 d was predictive of a plasma volume elevation,and areas under receiver operating characteristic curve were 0.686,0.721,0.647,and 0.766,respectively.The cumulative fluid balance for 4～7 d in the aggravated clinical congestion symptoms group was higher than that in the alleviated clinical congestion symptoms group,and the difference was statistically significant(P<0.05);the cumulative fluid balance for 4～7 d was an independent risk factor for the aggravated clinical congestion symptoms,and a cumulative fluid balance for 4～7 d of 2.574,3.383,4.995,and 4.235 L predicted aggravated clinical congestion symptoms,and area under receiver operating characteristic curve was 0.640,0.693,0.654,and 0.720,respectively.Conclusion The incidence of cumulative positive fluid balance in heart failure patients is high,and the amount of cumulative fluid balance can better predict the occurrence of volume overload,and the increase of plasma volume precedes the appearance of clinical congestion symptoms.It is suggested that heart failure patients with more than 3 d of cumulative positive balance should be closely monitored,and when the cumulative positive balance exceeds 2.308 L,measures should be taken in time to drain excessive fluid,so as to avoid the incidence of volume overload.