Background:Ulcerative colitis(UC),a chronic non-specific inflammatory bowel disease,is characterized by a prolonged course and a high tendency to relapse.Currently,there is no specific drug for its treatment.An increasing number of studies have revealed that washed microbiota transplantation(WMT)exhibits certain therapeutic effects on UC.Aims:To investigate the clinical efficacy and safety of WMT in treating UC,and to provide a reference basis for the selection of transplantation routes in the future.Methods:The clinical data of 49 UC patients who underwent WMT at the First Affiliated Hospital of Guangdong Pharmaceutical University from May 2017 to December 2020 were retrospectively analyzed.Among them,19 patients were in the middle digestive tract route group and 30 patients were in the lower digestive tract route group.The modified Mayo score,intestinal barrier function(diamine oxidase,D-lactic acid,and bacterial endotoxin),blood parameters,and adverse reactions were compared between the two groups.Results:The modified Mayo score of both groups were significantly lower after treatment compared with those before treatment(P＜0.05).After treatment,in the middle digestive tract route group,the scores for defecation frequency,endoscopic findings,and physician assessment were all significantly decreased(P＜0.05);in the lower digestive tract route group,the scores for defecation frequency,hematochezia,and physician assessment were significantly decreased(P＜0.05).After treatment,the level of bacterial endotoxin in the middle digestive tract route group was significantly reduced(P＜0.05),while there were no significant differences in the levels of diamine oxidase and D-lactic acid(P＞0.05);in the lower digestive tract route group,there were no significant differences in the levels of diamine oxidase,D-lactic acid,and bacterial endotoxin compared with those before treatment(P＞0.05).After treatment,there were no significant changes in hemoglobin,white blood cell count,platelet count,AST,ALT,albumin,creatinine,and blood urea nitrogen in both groups compared with those before treatment,and no severe adverse reactions occurred.Conclusions:WMT demonstrates good clinical efficacy and safety in the treatment of UC.The middle digestive tract transplantation route is more effective in improving intestinal mucosal inflammation and reducing bacterial endotoxin,while the lower digestive tract transplantation route is more effective in reducing patients'hematochezia.This indicates that hematochezia,endoscopic findings,and bacterial endotoxin can be used as references for the selection of transplantation routes.

Background:Ulcerative colitis(UC),a chronic non-specific inflammatory bowel disease,is characterized by a prolonged course and a high tendency to relapse.Currently,there is no specific drug for its treatment.An increasing number of studies have revealed that washed microbiota transplantation(WMT)exhibits certain therapeutic effects on UC.Aims:To investigate the clinical efficacy and safety of WMT in treating UC,and to provide a reference basis for the selection of transplantation routes in the future.Methods:The clinical data of 49 UC patients who underwent WMT at the First Affiliated Hospital of Guangdong Pharmaceutical University from May 2017 to December 2020 were retrospectively analyzed.Among them,19 patients were in the middle digestive tract route group and 30 patients were in the lower digestive tract route group.The modified Mayo score,intestinal barrier function(diamine oxidase,D-lactic acid,and bacterial endotoxin),blood parameters,and adverse reactions were compared between the two groups.Results:The modified Mayo score of both groups were significantly lower after treatment compared with those before treatment(P＜0.05).After treatment,in the middle digestive tract route group,the scores for defecation frequency,endoscopic findings,and physician assessment were all significantly decreased(P＜0.05);in the lower digestive tract route group,the scores for defecation frequency,hematochezia,and physician assessment were significantly decreased(P＜0.05).After treatment,the level of bacterial endotoxin in the middle digestive tract route group was significantly reduced(P＜0.05),while there were no significant differences in the levels of diamine oxidase and D-lactic acid(P＞0.05);in the lower digestive tract route group,there were no significant differences in the levels of diamine oxidase,D-lactic acid,and bacterial endotoxin compared with those before treatment(P＞0.05).After treatment,there were no significant changes in hemoglobin,white blood cell count,platelet count,AST,ALT,albumin,creatinine,and blood urea nitrogen in both groups compared with those before treatment,and no severe adverse reactions occurred.Conclusions:WMT demonstrates good clinical efficacy and safety in the treatment of UC.The middle digestive tract transplantation route is more effective in improving intestinal mucosal inflammation and reducing bacterial endotoxin,while the lower digestive tract transplantation route is more effective in reducing patients'hematochezia.This indicates that hematochezia,endoscopic findings,and bacterial endotoxin can be used as references for the selection of transplantation routes.

Objective:This study aims to study the mental health status of people with different identities during the fight against COVID-19. Further, we propose psychological intervention strategies for people with different identities.Methods:857 participants from Guangdong province (215 identified as first-level population referring to the impact severity of the outbreak, 91 as second-level population, 120 as third-level population, and 431 as fourth level population) were cross-sectionally surveyed online from January 30 to February 28, 2020. The Generalized Anxiety Disorder Scale (GAD-7), the Patient Health Questionnaire-9 (PHQ-9), and the Perceived Stress Scale (PSS-10) were used to evaluate the mental health status of different populations. The Kruskal-Wallis H test was used to test the differences of the total scores of these three scales across populations at different levels, and the groups were compared pairwise with the Bonferroni method. Finally, the Wilcoxon rank sum test was used to further analyze the dimensions between the scales. Results:In all 857 subjects, the total scores of GAD-7, PHQ-9, and PSS-10 were 4(1,8), 4(1,9), and 16(11,19), respectively. There were statistically significant differences in the scores of GAD-7 ( H=15.235, P<0.01), PHQ-9 ( H=9.265, P=0.026), and PSS-10 ( H=8.435, P=0.049) among different levels of the population. For the score of GAD-7, the anxiety severity of the first-level crowd was higher than that of the second-level crowd and the fourth-level crowd. The pairwise comparisons were statistically significant ( Z=-2.932, -4.012, -2.949, P<0.005); there were significant between-group differences in domains of "becoming easily annoyed or irritable" and "seemingly terrible things will happen". For the score of PHQ-9, the depression severity of the first-level crowd and the second-level crowd was higher than that of the fourth-level crowd, and the pairwise comparisons were statistically significant ( Z=-3.387, -2.682, P<0.005). There were significant between-group differences in domains of "difficult to fall asleep or not awake", "feeling depressed" and other related domains. For the score of PSS-10, the stress severity of the third-level crowd was higher than that of the first-level crowd and the fourth-level crowd ( Z=-2.702, -3.693, -2.735, P<0.005). There were significant between-group differences in domains of "feel confident", "life is as expected", "the ability to control anger", "the things are all under control", and other related domains. Conclusion:The impact of the COVID-19 on mental health in people with different identities is different, and appropriate psychological interventions should be provided for different populations according to their mental health status.

Objective:This study aims to study the mental health status of people with different identities during the fight against COVID-19. Further, we propose psychological intervention strategies for people with different identities.Methods:857 participants from Guangdong province (215 identified as first-level population referring to the impact severity of the outbreak, 91 as second-level population, 120 as third-level population, and 431 as fourth level population) were cross-sectionally surveyed online from January 30 to February 28, 2020. The Generalized Anxiety Disorder Scale (GAD-7), the Patient Health Questionnaire-9 (PHQ-9), and the Perceived Stress Scale (PSS-10) were used to evaluate the mental health status of different populations. The Kruskal-Wallis H test was used to test the differences of the total scores of these three scales across populations at different levels, and the groups were compared pairwise with the Bonferroni method. Finally, the Wilcoxon rank sum test was used to further analyze the dimensions between the scales. Results:In all 857 subjects, the total scores of GAD-7, PHQ-9, and PSS-10 were 4(1,8), 4(1,9), and 16(11,19), respectively. There were statistically significant differences in the scores of GAD-7 ( H=15.235, P<0.01), PHQ-9 ( H=9.265, P=0.026), and PSS-10 ( H=8.435, P=0.049) among different levels of the population. For the score of GAD-7, the anxiety severity of the first-level crowd was higher than that of the second-level crowd and the fourth-level crowd. The pairwise comparisons were statistically significant ( Z=-2.932, -4.012, -2.949, P<0.005); there were significant between-group differences in domains of "becoming easily annoyed or irritable" and "seemingly terrible things will happen". For the score of PHQ-9, the depression severity of the first-level crowd and the second-level crowd was higher than that of the fourth-level crowd, and the pairwise comparisons were statistically significant ( Z=-3.387, -2.682, P<0.005). There were significant between-group differences in domains of "difficult to fall asleep or not awake", "feeling depressed" and other related domains. For the score of PSS-10, the stress severity of the third-level crowd was higher than that of the first-level crowd and the fourth-level crowd ( Z=-2.702, -3.693, -2.735, P<0.005). There were significant between-group differences in domains of "feel confident", "life is as expected", "the ability to control anger", "the things are all under control", and other related domains. Conclusion:The impact of the COVID-19 on mental health in people with different identities is different, and appropriate psychological interventions should be provided for different populations according to their mental health status.